LOCUS       sequence-375513-        3850 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pENTRY for Gateway LR reaction.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3850)
  AUTHORS   Landry D, Mila I, Sabbagh CRR, Zaffuto M, Pouzet C, Tremousaygue D, 
            Dabos P, Deslandes L, Peeters N
  TITLE     An NLR integrated domain toolkit to identify plant pathogen effector
            targets.
  JOURNAL   Plant J. 2023 Sep;115(5):1443-1457. doi: 10.1111/tpj.16331. Epub 
            2023 Jul 24.
  PUBMED    37248633
REFERENCE   2  (bases 1 to 3850)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/tpj.16331";
            journalName: "Plant J"; date: "2023-09"; volume: "115"; issue: "5"; 
            pages: "1443-1457"
COMMENT     Sequence Label: pDL73
FEATURES             Location/Qualifiers
     source          1..3850
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    28..127
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(608..707)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(819..838)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(897..916)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     CDS             complement(1490..2023)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(2212..2217,2218..2234,2235..2240))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     rep_origin      2897..3485
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3386..3405
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(3620..3647)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3739..3825)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 gttaacgcta gcatggatct cgggccccaa ataatgattt tattttgact gatagtgacc
       61 tgttcgttgc aacaaattga tgagcaatgc ttttttataa tgccaacttt gtacaaaaaa
      121 gcaggcttcg aaggagatag aaccatgggg actcttgtac accacatgga actgctatgt
      181 cctattacgg ataaccacaa agataaagtt gctttcaagg atcgaattat aatgctcaac
      241 ttcttgagag aaaatctcat caatctacca agagaggccc ttgaagattt tgataccgca
      301 atcattaatg tagggcttct agtttactcg atgtatgata gtgtggatgc atcagttagt
      361 atacaagcac tgatatattt cctcacttgg aagtcatttc actacaattt acctaagatt
      421 gatggactgg gatctattga taccattata gaccacctga aggagtttct gagccgctac
      481 gtagagtcac tttcttctat aaggagccaa cttcagaaaa ttaagcagca attggagcag
      541 catgatggat tcggatcttt ggcaatgcag attacaagca tgagcgaaga aatggtgggt
      601 tttgatgacc cagctttctt gtacaaagtt ggcattataa gaaagcattg cttatcaatt
      661 tgttgcaacg aacaggtcac tatcagtcaa aataaaatca ttatttgcca tccagctgca
      721 gctctggccc gtgtctcaaa atctctgatg ttacattgca caagataaaa atatatcatc
      781 atgaacaata aaactgtctg cttacataaa cagtaataca aggggtgtta tgagccatat
      841 tcaacgggaa acgtcgaggc cgcgattaaa ttccaacatg gatgctgatt tatatgggta
      901 taaatgggct cgcgataatg tcgggcaatc aggtgcgaca atctatcgct tgtatgggaa
      961 gcccgatgcg ccagagttgt ttctgaaaca tggcaaaggt agcgttgcca atgatgttac
     1021 agatgagatg gtcagactaa actggctgac ggaatttatg cctcttccga ccatcaagca
     1081 ttttatccgt actcctgatg atgcatggtt actcaccact gcgatccccg gaaaaacagc
     1141 attccaggta ttagaagaat atcctgattc aggtgaaaat attgttgatg cgctggcagt
     1201 gttcctgcgc cggttgcatt cgattcctgt ttgtaattgt ccttttaaca gcgatcgcgt
     1261 atttcgtctc gctcaggcgc aatcacgaat gaataacggt ttggttgatg cgagtgattt
     1321 tgatgacgag cgtaatggct ggcctgttga acaagtctgg aaagaaatgc ataaactttt
     1381 gccattctca ccggattcag tcgtcactca tggtgatttc tcacttgata accttatttt
     1441 tgacgagggg aaattaggcc gggaagccga tctcggcttg aacgaattgt taggtggcgg
     1501 tacttgggtc gatatcaaag tgcatcactt cttcccgtat gcccaacttt gtatagagag
     1561 ccactgcggg atcgtcaccg taatctgctt gcacgtagat cacataagca ccaagcgcgt
     1621 tggcctcatg cttgaggaga ttgatgagcg cggtggcaat gccctgcctc cggtgctcgc
     1681 cggagactgc gagatcatag atatagatct cactacgcgg ctgctcaaac ttgggcagaa
     1741 cgtaagccgc gagagcgcca acaaccgctt cttggtcgaa ggcagcaagc gcgatgaatg
     1801 tcttactacg gagcaagttc ccgaggtaat cggagtccgg ctgatgttgg gagtaggtgg
     1861 ctacgtctcc gaactcacga ccgaaaagat caagagcagc ccgcatggat ttgacttggt
     1921 cagggccgag cctacatgtg cgaatgatgc ccatacttga gccacctaac tttgttttag
     1981 ggcgactgcc ctgctgcgta acatcgttgc tgctgcgtaa catcgttgct gctccataac
     2041 atcaaacatc gacccacggc gtaacgcgct tgctgcttgg atgcccgagg catagactgt
     2101 acaaaaaaac agtcataaca agccatgaaa accgccactg cgccgttacc accgctgcgt
     2161 tcggtcaagg ttctggacca gttgcgtgag cgcatacgct acttgcatta cagtttacga
     2221 accgaacagg cttatgtcaa ctgggttcgt gccttcatcc gtttccacgg tgtgcgtcac
     2281 ccggcaacct tgggcagcag cgaagtcgag gcatttctgt cctggctggc gaacgagcgc
     2341 aaggtttcgg tctccacgca tcgtcaggca ttggcggcct tgctgttctt ctacggcaag
     2401 gtgctgtgca cggatctgcc ctggcttcag gagatcggaa gacctcggcc gtcgcggcgc
     2461 ttgccggtgg tgctgacccc ggatgaagtg gttcgcatcc tcggttttct ggaaggcgag
     2521 catcgtttgt tcgcccagga ctctagctat agttctagtg gttggctact aataggttgt
     2581 attgatgttg gacgagtcgg aatcgcagac cgataccagg atcttgccat cctatggaac
     2641 tgcctcggtg agttttctcc ttcattacag aaacggcttt ttcaaaaata tggtattgat
     2701 aatcctgata tgaataaatt gcagtttcat ttgatgctcg atgagttttt ctaatcagaa
     2761 ttggttaatt ggttgtaaca ctggcagagc attacgctga cttgacggga cggcgcaagc
     2821 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     2881 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     2941 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     3001 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
     3061 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     3121 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     3181 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     3241 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     3301 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     3361 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     3421 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     3481 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     3541 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gctagccagg
     3601 aagagtttgt agaaacgcaa aaaggccatc cgtcaggatg gccttctgct tagtttgatg
     3661 cctggcagtt tatggcgggc gtcctgcccg ccaccctccg ggccgttgct tcacaacgtt
     3721 caaatccgct cccggcggat ttgtcctact caggagagcg ttcaccgaca aacaacagat
     3781 aaaacgaaag gcccagtctt ccgactgagc ctttcgtttt atttgatgcc tggcagttcc
     3841 ctactctcgc
//
