LOCUS       sequence-375514-        4216 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pENTRY for Gateway LR reaction.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4216)
  AUTHORS   Landry D, Mila I, Sabbagh CRR, Zaffuto M, Pouzet C, Tremousaygue D, 
            Dabos P, Deslandes L, Peeters N
  TITLE     An NLR integrated domain toolkit to identify plant pathogen effector
            targets.
  JOURNAL   Plant J. 2023 Sep;115(5):1443-1457. doi: 10.1111/tpj.16331. Epub 
            2023 Jul 24.
  PUBMED    37248633
REFERENCE   2  (bases 1 to 4216)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/tpj.16331";
            journalName: "Plant J"; date: "2023-09"; volume: "115"; issue: "5"; 
            pages: "1443-1457"
COMMENT     Sequence Label: pDL106
FEATURES             Location/Qualifiers
     source          1..4216
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    28..127
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(974..1073)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1185..1204)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(1263..1282)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     CDS             complement(1856..2389)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(2578..2583,2584..2600,2601..2606))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     rep_origin      3263..3851
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3752..3771
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(3986..4013)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4105..4191)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 gttaacgcta gcatggatct cgggccccaa ataatgattt tattttgact gatagtgacc
       61 tgttcgttgc aacaaattga tgagcaatgc ttttttataa tgccaacttt gtacaaaaaa
      121 gcaggcttcg aaggagatag aaccatggat gattggagaa gtctggattc catttatatt
      181 ttagaaaggt cttcacctcg gtcgtcaagc ctccaatcac ttcggatcaa atcccataat
      241 tcaattgaat tatttgaagt caagcttaag atggacatgc tcaccgctct tgaagatttg
      301 catatgaaat gccaaaagtt gtcattttct gaaggagttt gtctacctcc caaattaaga
      361 acaattgtga tttctaccaa aaaaacagca ccgccggtta cggaatgggg tctccaatat
      421 ttgacagctc tttcatcatt gtggattgta aagggtgatg atatatttaa caccttgatg
      481 aaggagtcat tgctacccat ctcccttgtg tctctaaata tcatggttct ctctgaaatg
      541 aagtcctttg atggaaatgg gcttcgacac ctcttctctc tccaatatct ctattttgcg
      601 ggttgtcaac agcttgggtc attgccagaa aactgcttcc cttcctcact gaaatcactt
      661 aaatttgttg attgtaagaa gcttgagtta attccagtaa actgcctccc atcatcgctg
      721 aaatcactta aatttgttga ttgtaagaag cttgagtcat taccagaaaa ctgcctccca
      781 tcatcgctga aatcacttga attgtggaaa tgtgaaaagc tagagtcatt accagaagac
      841 agcctccctg actcactgaa gagactggac atttatggat gtccattgtt agaagaaagg
      901 tataaaagga aggaacattg gtccaaaatt gctcacatcc ctgtcataga aataaatgac
      961 caggttacaa tagacccagc tttcttgtac aaagttggca ttataagaaa gcattgctta
     1021 tcaatttgtt gcaacgaaca ggtcactatc agtcaaaata aaatcattat ttgccatcca
     1081 gctgcagctc tggcccgtgt ctcaaaatct ctgatgttac attgcacaag ataaaaatat
     1141 atcatcatga acaataaaac tgtctgctta cataaacagt aatacaaggg gtgttatgag
     1201 ccatattcaa cgggaaacgt cgaggccgcg attaaattcc aacatggatg ctgatttata
     1261 tgggtataaa tgggctcgcg ataatgtcgg gcaatcaggt gcgacaatct atcgcttgta
     1321 tgggaagccc gatgcgccag agttgtttct gaaacatggc aaaggtagcg ttgccaatga
     1381 tgttacagat gagatggtca gactaaactg gctgacggaa tttatgcctc ttccgaccat
     1441 caagcatttt atccgtactc ctgatgatgc atggttactc accactgcga tccccggaaa
     1501 aacagcattc caggtattag aagaatatcc tgattcaggt gaaaatattg ttgatgcgct
     1561 ggcagtgttc ctgcgccggt tgcattcgat tcctgtttgt aattgtcctt ttaacagcga
     1621 tcgcgtattt cgtctcgctc aggcgcaatc acgaatgaat aacggtttgg ttgatgcgag
     1681 tgattttgat gacgagcgta atggctggcc tgttgaacaa gtctggaaag aaatgcataa
     1741 acttttgcca ttctcaccgg attcagtcgt cactcatggt gatttctcac ttgataacct
     1801 tatttttgac gaggggaaat taggccggga agccgatctc ggcttgaacg aattgttagg
     1861 tggcggtact tgggtcgata tcaaagtgca tcacttcttc ccgtatgccc aactttgtat
     1921 agagagccac tgcgggatcg tcaccgtaat ctgcttgcac gtagatcaca taagcaccaa
     1981 gcgcgttggc ctcatgcttg aggagattga tgagcgcggt ggcaatgccc tgcctccggt
     2041 gctcgccgga gactgcgaga tcatagatat agatctcact acgcggctgc tcaaacttgg
     2101 gcagaacgta agccgcgaga gcgccaacaa ccgcttcttg gtcgaaggca gcaagcgcga
     2161 tgaatgtctt actacggagc aagttcccga ggtaatcgga gtccggctga tgttgggagt
     2221 aggtggctac gtctccgaac tcacgaccga aaagatcaag agcagcccgc atggatttga
     2281 cttggtcagg gccgagccta catgtgcgaa tgatgcccat acttgagcca cctaactttg
     2341 ttttagggcg actgccctgc tgcgtaacat cgttgctgct gcgtaacatc gttgctgctc
     2401 cataacatca aacatcgacc cacggcgtaa cgcgcttgct gcttggatgc ccgaggcata
     2461 gactgtacaa aaaaacagtc ataacaagcc atgaaaaccg ccactgcgcc gttaccaccg
     2521 ctgcgttcgg tcaaggttct ggaccagttg cgtgagcgca tacgctactt gcattacagt
     2581 ttacgaaccg aacaggctta tgtcaactgg gttcgtgcct tcatccgttt ccacggtgtg
     2641 cgtcacccgg caaccttggg cagcagcgaa gtcgaggcat ttctgtcctg gctggcgaac
     2701 gagcgcaagg tttcggtctc cacgcatcgt caggcattgg cggccttgct gttcttctac
     2761 ggcaaggtgc tgtgcacgga tctgccctgg cttcaggaga tcggaagacc tcggccgtcg
     2821 cggcgcttgc cggtggtgct gaccccggat gaagtggttc gcatcctcgg ttttctggaa
     2881 ggcgagcatc gtttgttcgc ccaggactct agctatagtt ctagtggttg gctactaata
     2941 ggttgtattg atgttggacg agtcggaatc gcagaccgat accaggatct tgccatccta
     3001 tggaactgcc tcggtgagtt ttctccttca ttacagaaac ggctttttca aaaatatggt
     3061 attgataatc ctgatatgaa taaattgcag tttcatttga tgctcgatga gtttttctaa
     3121 tcagaattgg ttaattggtt gtaacactgg cagagcatta cgctgacttg acgggacggc
     3181 gcaagctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     3241 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     3301 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     3361 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
     3421 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     3481 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     3541 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     3601 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     3661 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     3721 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     3781 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     3841 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     3901 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcta
     3961 gccaggaaga gtttgtagaa acgcaaaaag gccatccgtc aggatggcct tctgcttagt
     4021 ttgatgcctg gcagtttatg gcgggcgtcc tgcccgccac cctccgggcc gttgcttcac
     4081 aacgttcaaa tccgctcccg gcggatttgt cctactcagg agagcgttca ccgacaaaca
     4141 acagataaaa cgaaaggccc agtcttccga ctgagccttt cgttttattt gatgcctggc
     4201 agttccctac tctcgc
//
