LOCUS       sequence-375515-        4909 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pENTRY for Gateway LR reaction.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4909)
  AUTHORS   Landry D, Mila I, Sabbagh CRR, Zaffuto M, Pouzet C, Tremousaygue D, 
            Dabos P, Deslandes L, Peeters N
  TITLE     An NLR integrated domain toolkit to identify plant pathogen effector
            targets.
  JOURNAL   Plant J. 2023 Sep;115(5):1443-1457. doi: 10.1111/tpj.16331. Epub 
            2023 Jul 24.
  PUBMED    37248633
REFERENCE   2  (bases 1 to 4909)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/tpj.16331";
            journalName: "Plant J"; date: "2023-09"; volume: "115"; issue: "5"; 
            pages: "1443-1457"
COMMENT     Sequence Label: pDL69
FEATURES             Location/Qualifiers
     source          1..4909
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    28..127
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1667..1766)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1878..1897)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(1956..1975)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     CDS             complement(2549..3082)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(3271..3276,3277..3293,3294..3299))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     rep_origin      3956..4544
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4445..4464
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(4679..4706)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4798..4884)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 gttaacgcta gcatggatct cgggccccaa ataatgattt tattttgact gatagtgacc
       61 tgttcgttgc aacaaattga tgagcaatgc ttttttataa tgccaacttt gtacaaaaaa
      121 gcaggcttcg aaggagatag aaccatgtcg aatttgtgta gagggagata ttttgacatt
      181 tctcctttgg aaaagattgt ccatgaatgg gatttaggca gctcgatgga gcataaaatg
      241 gactgcctta atatcgattt tcaatttcta aatatgcttc tcagcttgca gagcttcaca
      301 agaagagact gtttggatac tacagtagag aaagtggaag ctctttggga tgcagctgaa
      361 tttgaacgaa tcaactgtat cgacgatgtt aaactcgtgt tggataagat ggaagaagag
      421 atatgggaaa ctaaaattca tatcgaagct aaatactcat ttcccatagc acttatttcc
      481 aaaattgcta ctctcgatcc cgaatttgtg aagagattca ttgatacagt tataatgaat
      541 atcacggatt taccaaagat aaatcgtctt tatacatggt ctagattaaa acaaatacaa
      601 gaggctgtaa aggagttgaa gttattgaga aattttgtct attttatttc agacagatgc
      661 acgaatcctc aaagccaata tactttcttc agtcacgttt tagttgtggc tggtcatgta
      721 gcaatgattt cctggtttag tatcaacgcc gatatgaact ttttctttga tccacttaaa
      781 ttggatgctt tattttctga ccttcaaatg aggattcagc ccattcaacc aggtatccac
      841 gagatctata tcgatgtcct gcaagctcta aaatcaggat ctcatccaaa tatcgatgtc
      901 catgctgctg attataaagc tggctttgtg gagactctcg tacaccatat ggaagtgcta
      961 cgtcccatta ttcataagca cgaaaaggga gttgctttca aggatcaaat tacaatgctc
     1021 aacttcttga gagacaatct catcaatcta ccaagagagg ccgttgaaga ttttgatact
     1081 ggaattatta atgtagggct tctagtttac tcggtgttta aaagtgtggc tcttgggaag
     1141 tcaaaacgat tagttccagt tcttgatttc tcaggtgata ttcagagtat acaagcacta
     1201 gtctacttcc tcactcggaa ttcattttgc tccaatttac ctaagattga cggactggga
     1261 tctattgata tcattttaga ccaactggag gagtttctaa gctgttactc agagttaact
     1321 tcttctataa ggagccaaat tcagaaaata cagcagcaac tggagcattt ccagaagcag
     1381 aatgatggat tcggatcttt tgggatgcag gtgattacta aagcatatga ggtggatcac
     1441 gtggttgttg gttatataaa caaagacatt cctgagtggt gtctttttct gtggactcgg
     1501 gacatcatag aggagattac acaactaatc gaggcagggg agattcatgg aaaggaagtc
     1561 tctgactcgg tattgcataa taccactgat gttgccaatg ctgatacttc acaacttgct
     1621 cagatgacaa gcatgagcga agagatggtg ggctttcagg acgtggaccc agctttcttg
     1681 tacaaagttg gcattataag aaagcattgc ttatcaattt gttgcaacga acaggtcact
     1741 atcagtcaaa ataaaatcat tatttgccat ccagctgcag ctctggcccg tgtctcaaaa
     1801 tctctgatgt tacattgcac aagataaaaa tatatcatca tgaacaataa aactgtctgc
     1861 ttacataaac agtaatacaa ggggtgttat gagccatatt caacgggaaa cgtcgaggcc
     1921 gcgattaaat tccaacatgg atgctgattt atatgggtat aaatgggctc gcgataatgt
     1981 cgggcaatca ggtgcgacaa tctatcgctt gtatgggaag cccgatgcgc cagagttgtt
     2041 tctgaaacat ggcaaaggta gcgttgccaa tgatgttaca gatgagatgg tcagactaaa
     2101 ctggctgacg gaatttatgc ctcttccgac catcaagcat tttatccgta ctcctgatga
     2161 tgcatggtta ctcaccactg cgatccccgg aaaaacagca ttccaggtat tagaagaata
     2221 tcctgattca ggtgaaaata ttgttgatgc gctggcagtg ttcctgcgcc ggttgcattc
     2281 gattcctgtt tgtaattgtc cttttaacag cgatcgcgta tttcgtctcg ctcaggcgca
     2341 atcacgaatg aataacggtt tggttgatgc gagtgatttt gatgacgagc gtaatggctg
     2401 gcctgttgaa caagtctgga aagaaatgca taaacttttg ccattctcac cggattcagt
     2461 cgtcactcat ggtgatttct cacttgataa ccttattttt gacgagggga aattaggccg
     2521 ggaagccgat ctcggcttga acgaattgtt aggtggcggt acttgggtcg atatcaaagt
     2581 gcatcacttc ttcccgtatg cccaactttg tatagagagc cactgcggga tcgtcaccgt
     2641 aatctgcttg cacgtagatc acataagcac caagcgcgtt ggcctcatgc ttgaggagat
     2701 tgatgagcgc ggtggcaatg ccctgcctcc ggtgctcgcc ggagactgcg agatcataga
     2761 tatagatctc actacgcggc tgctcaaact tgggcagaac gtaagccgcg agagcgccaa
     2821 caaccgcttc ttggtcgaag gcagcaagcg cgatgaatgt cttactacgg agcaagttcc
     2881 cgaggtaatc ggagtccggc tgatgttggg agtaggtggc tacgtctccg aactcacgac
     2941 cgaaaagatc aagagcagcc cgcatggatt tgacttggtc agggccgagc ctacatgtgc
     3001 gaatgatgcc catacttgag ccacctaact ttgttttagg gcgactgccc tgctgcgtaa
     3061 catcgttgct gctgcgtaac atcgttgctg ctccataaca tcaaacatcg acccacggcg
     3121 taacgcgctt gctgcttgga tgcccgaggc atagactgta caaaaaaaca gtcataacaa
     3181 gccatgaaaa ccgccactgc gccgttacca ccgctgcgtt cggtcaaggt tctggaccag
     3241 ttgcgtgagc gcatacgcta cttgcattac agtttacgaa ccgaacaggc ttatgtcaac
     3301 tgggttcgtg ccttcatccg tttccacggt gtgcgtcacc cggcaacctt gggcagcagc
     3361 gaagtcgagg catttctgtc ctggctggcg aacgagcgca aggtttcggt ctccacgcat
     3421 cgtcaggcat tggcggcctt gctgttcttc tacggcaagg tgctgtgcac ggatctgccc
     3481 tggcttcagg agatcggaag acctcggccg tcgcggcgct tgccggtggt gctgaccccg
     3541 gatgaagtgg ttcgcatcct cggttttctg gaaggcgagc atcgtttgtt cgcccaggac
     3601 tctagctata gttctagtgg ttggctacta ataggttgta ttgatgttgg acgagtcgga
     3661 atcgcagacc gataccagga tcttgccatc ctatggaact gcctcggtga gttttctcct
     3721 tcattacaga aacggctttt tcaaaaatat ggtattgata atcctgatat gaataaattg
     3781 cagtttcatt tgatgctcga tgagtttttc taatcagaat tggttaattg gttgtaacac
     3841 tggcagagca ttacgctgac ttgacgggac ggcgcaagct catgaccaaa atcccttaac
     3901 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     3961 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     4021 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     4081 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
     4141 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     4201 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     4261 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     4321 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     4381 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     4441 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     4501 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     4561 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     4621 cccctgattc tgtggataac cgtattaccg ctagccagga agagtttgta gaaacgcaaa
     4681 aaggccatcc gtcaggatgg ccttctgctt agtttgatgc ctggcagttt atggcgggcg
     4741 tcctgcccgc caccctccgg gccgttgctt cacaacgttc aaatccgctc ccggcggatt
     4801 tgtcctactc aggagagcgt tcaccgacaa acaacagata aaacgaaagg cccagtcttc
     4861 cgactgagcc tttcgtttta tttgatgcct ggcagttccc tactctcgc
//
