LOCUS       sequence-375517-        4090 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pENTRY for Gateway LR reaction.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4090)
  AUTHORS   Landry D, Mila I, Sabbagh CRR, Zaffuto M, Pouzet C, Tremousaygue D, 
            Dabos P, Deslandes L, Peeters N
  TITLE     An NLR integrated domain toolkit to identify plant pathogen effector
            targets.
  JOURNAL   Plant J. 2023 Sep;115(5):1443-1457. doi: 10.1111/tpj.16331. Epub 
            2023 Jul 24.
  PUBMED    37248633
REFERENCE   2  (bases 1 to 4090)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/tpj.16331";
            journalName: "Plant J"; date: "2023-09"; volume: "115"; issue: "5"; 
            pages: "1443-1457"
COMMENT     Sequence Label: pDL65
FEATURES             Location/Qualifiers
     source          1..4090
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    28..127
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(848..947)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1059..1078)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(1137..1156)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     CDS             complement(1730..2263)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(2452..2457,2458..2474,2475..2480))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     rep_origin      3137..3725
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3626..3645
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(3860..3887)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3979..4065)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 gttaacgcta gcatggatct cgggccccaa ataatgattt tattttgact gatagtgacc
       61 tgttcgttgc aacaaattga tgagcaatgc ttttttataa tgccaacttt gtacaaaaaa
      121 gcaggcttcg aaggagatag aaccatgata attcctcaat tccagaacat acgcacaatg
      181 actattcatc ggtgcgaata tcttagagac ttgacatggt tgctattagc cccgtgtctt
      241 ggtgagctaa gtgtatctga atgtccgcaa atggaagaag taataagcaa agataaagct
      301 atggccaagc tgggtaatac gagtgagcag ccctttcaaa atctaactaa gctcgtctta
      361 gatggtttac ctaaactgga gagcatctac tggactcctc tacccttccc agttctggaa
      421 tatttagtga taaggcgttg tccagagctg agaagacttc cattcaactc tgagagcact
      481 ataggaaatc aagttgaaac gataattgag gagcaagtga taaaaatagt tgaatgggag
      541 gatgaagcta caaaacaacg tttctcccat ttcaataaca gagactttgt acagatggct
      601 gaagatccga agatggatgg tttgacatcg gagtcacatc caattcaaac catagacctg
      661 gtcgggacta caggaagtgg agaaactgcc actgcaaaca acatccaagg aaagaaggtg
      721 gtccaatcgg gaacacacgc aactgttgtt accatggaat gccagacata taaagttttc
      781 acaccagatt gccccatcaa caatatgatt gacactcctg gtacgaattt ccttttatgt
      841 tataccgacc cagctttctt gtacaaagtt ggcattataa gaaagcattg cttatcaatt
      901 tgttgcaacg aacaggtcac tatcagtcaa aataaaatca ttatttgcca tccagctgca
      961 gctctggccc gtgtctcaaa atctctgatg ttacattgca caagataaaa atatatcatc
     1021 atgaacaata aaactgtctg cttacataaa cagtaataca aggggtgtta tgagccatat
     1081 tcaacgggaa acgtcgaggc cgcgattaaa ttccaacatg gatgctgatt tatatgggta
     1141 taaatgggct cgcgataatg tcgggcaatc aggtgcgaca atctatcgct tgtatgggaa
     1201 gcccgatgcg ccagagttgt ttctgaaaca tggcaaaggt agcgttgcca atgatgttac
     1261 agatgagatg gtcagactaa actggctgac ggaatttatg cctcttccga ccatcaagca
     1321 ttttatccgt actcctgatg atgcatggtt actcaccact gcgatccccg gaaaaacagc
     1381 attccaggta ttagaagaat atcctgattc aggtgaaaat attgttgatg cgctggcagt
     1441 gttcctgcgc cggttgcatt cgattcctgt ttgtaattgt ccttttaaca gcgatcgcgt
     1501 atttcgtctc gctcaggcgc aatcacgaat gaataacggt ttggttgatg cgagtgattt
     1561 tgatgacgag cgtaatggct ggcctgttga acaagtctgg aaagaaatgc ataaactttt
     1621 gccattctca ccggattcag tcgtcactca tggtgatttc tcacttgata accttatttt
     1681 tgacgagggg aaattaggcc gggaagccga tctcggcttg aacgaattgt taggtggcgg
     1741 tacttgggtc gatatcaaag tgcatcactt cttcccgtat gcccaacttt gtatagagag
     1801 ccactgcggg atcgtcaccg taatctgctt gcacgtagat cacataagca ccaagcgcgt
     1861 tggcctcatg cttgaggaga ttgatgagcg cggtggcaat gccctgcctc cggtgctcgc
     1921 cggagactgc gagatcatag atatagatct cactacgcgg ctgctcaaac ttgggcagaa
     1981 cgtaagccgc gagagcgcca acaaccgctt cttggtcgaa ggcagcaagc gcgatgaatg
     2041 tcttactacg gagcaagttc ccgaggtaat cggagtccgg ctgatgttgg gagtaggtgg
     2101 ctacgtctcc gaactcacga ccgaaaagat caagagcagc ccgcatggat ttgacttggt
     2161 cagggccgag cctacatgtg cgaatgatgc ccatacttga gccacctaac tttgttttag
     2221 ggcgactgcc ctgctgcgta acatcgttgc tgctgcgtaa catcgttgct gctccataac
     2281 atcaaacatc gacccacggc gtaacgcgct tgctgcttgg atgcccgagg catagactgt
     2341 acaaaaaaac agtcataaca agccatgaaa accgccactg cgccgttacc accgctgcgt
     2401 tcggtcaagg ttctggacca gttgcgtgag cgcatacgct acttgcatta cagtttacga
     2461 accgaacagg cttatgtcaa ctgggttcgt gccttcatcc gtttccacgg tgtgcgtcac
     2521 ccggcaacct tgggcagcag cgaagtcgag gcatttctgt cctggctggc gaacgagcgc
     2581 aaggtttcgg tctccacgca tcgtcaggca ttggcggcct tgctgttctt ctacggcaag
     2641 gtgctgtgca cggatctgcc ctggcttcag gagatcggaa gacctcggcc gtcgcggcgc
     2701 ttgccggtgg tgctgacccc ggatgaagtg gttcgcatcc tcggttttct ggaaggcgag
     2761 catcgtttgt tcgcccagga ctctagctat agttctagtg gttggctact aataggttgt
     2821 attgatgttg gacgagtcgg aatcgcagac cgataccagg atcttgccat cctatggaac
     2881 tgcctcggtg agttttctcc ttcattacag aaacggcttt ttcaaaaata tggtattgat
     2941 aatcctgata tgaataaatt gcagtttcat ttgatgctcg atgagttttt ctaatcagaa
     3001 ttggttaatt ggttgtaaca ctggcagagc attacgctga cttgacggga cggcgcaagc
     3061 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     3121 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     3181 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     3241 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
     3301 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     3361 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     3421 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     3481 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     3541 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     3601 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     3661 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     3721 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     3781 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gctagccagg
     3841 aagagtttgt agaaacgcaa aaaggccatc cgtcaggatg gccttctgct tagtttgatg
     3901 cctggcagtt tatggcgggc gtcctgcccg ccaccctccg ggccgttgct tcacaacgtt
     3961 caaatccgct cccggcggat ttgtcctact caggagagcg ttcaccgaca aacaacagat
     4021 aaaacgaaag gcccagtctt ccgactgagc ctttcgtttt atttgatgcc tggcagttcc
     4081 ctactctcgc
//
