LOCUS       sequence-375518-        3925 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pENTRY for Gateway LR reaction.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3925)
  AUTHORS   Landry D, Mila I, Sabbagh CRR, Zaffuto M, Pouzet C, Tremousaygue D, 
            Dabos P, Deslandes L, Peeters N
  TITLE     An NLR integrated domain toolkit to identify plant pathogen effector
            targets.
  JOURNAL   Plant J. 2023 Sep;115(5):1443-1457. doi: 10.1111/tpj.16331. Epub 
            2023 Jul 24.
  PUBMED    37248633
REFERENCE   2  (bases 1 to 3925)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/tpj.16331";
            journalName: "Plant J"; date: "2023-09"; volume: "115"; issue: "5"; 
            pages: "1443-1457"
COMMENT     Sequence Label: pDL109
FEATURES             Location/Qualifiers
     source          1..3925
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    28..127
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(683..782)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(894..913)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(972..991)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     CDS             complement(1565..2098)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(2287..2292,2293..2309,2310..2315))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     rep_origin      2972..3560
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3461..3480
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(3695..3722)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3814..3900)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 gttaacgcta gcatggatct cgggccccaa ataatgattt tattttgact gatagtgacc
       61 tgttcgttgc aacaaattga tgagcaatgc ttttttataa tgccaacttt gtacaaaaaa
      121 gcaggcttcg aaggagatag aaccatgtcc atgagagagt tggttggtgg agcgtttctt
      181 tcatcattct ttcaagtggc tcttgagaaa ttgtcttcca atgatttcat tgactatttc
      241 cgtagaagca aacttgatgt taatctgctg gagaaacttc taataacact caattctatc
      301 aaccatgtgt tggaagaagc ggagatgaag caataccaaa gcatgtacgt gaagaagtgg
      361 cttgatgatc ttaaacatta tgcatatgag gttgaccagc tgttagatga gattgcaact
      421 gatgcaccac tgaagaagct gaaagctgaa tctcaacctt ctactagcaa ggtattcgac
      481 ttcttttcat ctttcactaa tccatttgaa tctaggatta aagaattgct agagaagcta
      541 gaatttcttg caaagcaaaa ggatatgctc ggattgaaac atgaggcttt tgctagtagt
      601 gaaggtggcg ttagttggaa acccttagat agatttccaa ctacagcttt ggtggatgaa
      661 tcgagcatat atggtagaga tgacccagct ttcttgtaca aagttggcat tataagaaag
      721 cattgcttat caatttgttg caacgaacag gtcactatca gtcaaaataa aatcattatt
      781 tgccatccag ctgcagctct ggcccgtgtc tcaaaatctc tgatgttaca ttgcacaaga
      841 taaaaatata tcatcatgaa caataaaact gtctgcttac ataaacagta atacaagggg
      901 tgttatgagc catattcaac gggaaacgtc gaggccgcga ttaaattcca acatggatgc
      961 tgatttatat gggtataaat gggctcgcga taatgtcggg caatcaggtg cgacaatcta
     1021 tcgcttgtat gggaagcccg atgcgccaga gttgtttctg aaacatggca aaggtagcgt
     1081 tgccaatgat gttacagatg agatggtcag actaaactgg ctgacggaat ttatgcctct
     1141 tccgaccatc aagcatttta tccgtactcc tgatgatgca tggttactca ccactgcgat
     1201 ccccggaaaa acagcattcc aggtattaga agaatatcct gattcaggtg aaaatattgt
     1261 tgatgcgctg gcagtgttcc tgcgccggtt gcattcgatt cctgtttgta attgtccttt
     1321 taacagcgat cgcgtatttc gtctcgctca ggcgcaatca cgaatgaata acggtttggt
     1381 tgatgcgagt gattttgatg acgagcgtaa tggctggcct gttgaacaag tctggaaaga
     1441 aatgcataaa cttttgccat tctcaccgga ttcagtcgtc actcatggtg atttctcact
     1501 tgataacctt atttttgacg aggggaaatt aggccgggaa gccgatctcg gcttgaacga
     1561 attgttaggt ggcggtactt gggtcgatat caaagtgcat cacttcttcc cgtatgccca
     1621 actttgtata gagagccact gcgggatcgt caccgtaatc tgcttgcacg tagatcacat
     1681 aagcaccaag cgcgttggcc tcatgcttga ggagattgat gagcgcggtg gcaatgccct
     1741 gcctccggtg ctcgccggag actgcgagat catagatata gatctcacta cgcggctgct
     1801 caaacttggg cagaacgtaa gccgcgagag cgccaacaac cgcttcttgg tcgaaggcag
     1861 caagcgcgat gaatgtctta ctacggagca agttcccgag gtaatcggag tccggctgat
     1921 gttgggagta ggtggctacg tctccgaact cacgaccgaa aagatcaaga gcagcccgca
     1981 tggatttgac ttggtcaggg ccgagcctac atgtgcgaat gatgcccata cttgagccac
     2041 ctaactttgt tttagggcga ctgccctgct gcgtaacatc gttgctgctg cgtaacatcg
     2101 ttgctgctcc ataacatcaa acatcgaccc acggcgtaac gcgcttgctg cttggatgcc
     2161 cgaggcatag actgtacaaa aaaacagtca taacaagcca tgaaaaccgc cactgcgccg
     2221 ttaccaccgc tgcgttcggt caaggttctg gaccagttgc gtgagcgcat acgctacttg
     2281 cattacagtt tacgaaccga acaggcttat gtcaactggg ttcgtgcctt catccgtttc
     2341 cacggtgtgc gtcacccggc aaccttgggc agcagcgaag tcgaggcatt tctgtcctgg
     2401 ctggcgaacg agcgcaaggt ttcggtctcc acgcatcgtc aggcattggc ggccttgctg
     2461 ttcttctacg gcaaggtgct gtgcacggat ctgccctggc ttcaggagat cggaagacct
     2521 cggccgtcgc ggcgcttgcc ggtggtgctg accccggatg aagtggttcg catcctcggt
     2581 tttctggaag gcgagcatcg tttgttcgcc caggactcta gctatagttc tagtggttgg
     2641 ctactaatag gttgtattga tgttggacga gtcggaatcg cagaccgata ccaggatctt
     2701 gccatcctat ggaactgcct cggtgagttt tctccttcat tacagaaacg gctttttcaa
     2761 aaatatggta ttgataatcc tgatatgaat aaattgcagt ttcatttgat gctcgatgag
     2821 tttttctaat cagaattggt taattggttg taacactggc agagcattac gctgacttga
     2881 cgggacggcg caagctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     2941 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     3001 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     3061 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     3121 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     3181 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     3241 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     3301 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     3361 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     3421 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     3481 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     3541 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     3601 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     3661 ttaccgctag ccaggaagag tttgtagaaa cgcaaaaagg ccatccgtca ggatggcctt
     3721 ctgcttagtt tgatgcctgg cagtttatgg cgggcgtcct gcccgccacc ctccgggccg
     3781 ttgcttcaca acgttcaaat ccgctcccgg cggatttgtc ctactcagga gagcgttcac
     3841 cgacaaacaa cagataaaac gaaaggccca gtcttccgac tgagcctttc gttttatttg
     3901 atgcctggca gttccctact ctcgc
//
