LOCUS       sequence-375521-        4456 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pENTRY for Gateway LR reaction.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4456)
  AUTHORS   Landry D, Mila I, Sabbagh CRR, Zaffuto M, Pouzet C, Tremousaygue D, 
            Dabos P, Deslandes L, Peeters N
  TITLE     An NLR integrated domain toolkit to identify plant pathogen effector
            targets.
  JOURNAL   Plant J. 2023 Sep;115(5):1443-1457. doi: 10.1111/tpj.16331. Epub 
            2023 Jul 24.
  PUBMED    37248633
REFERENCE   2  (bases 1 to 4456)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/tpj.16331";
            journalName: "Plant J"; date: "2023-09"; volume: "115"; issue: "5"; 
            pages: "1443-1457"
COMMENT     Sequence Label: pDL201
FEATURES             Location/Qualifiers
     source          1..4456
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    28..127
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1214..1313)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1425..1444)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(1503..1522)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     CDS             complement(2096..2629)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(2818..2823,2824..2840,2841..2846))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     rep_origin      3503..4091
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3992..4011
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(4226..4253)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4345..4431)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 gttaacgcta gcatggatct cgggccccaa ataatgattt tattttgact gatagtgacc
       61 tgttcgttgc aacaaattga tgagcaatgc ttttttataa tgccaacttt gtacaaaaaa
      121 gcaggcttcg aaggagatag aaccatggaa gcttgccatt cacttggtgt catgcataga
      181 gatctgaaac ccgaaaactt cttgttggtt aacaaggaca acgatttttc tctcaaggcc
      241 attgattttg gactctccgt tttcttcaaa ccaggtcaag ttttcactga tgtggttgga
      301 agcccatatt atgttgctcc ggaggtactc ctcaagcatt atggaccaga agcagatgtg
      361 tggactgctg gagttatact gtatattctg ctcagcggcg tgccaccatt ttgggcagaa
      421 acccagcagg ggatctttga tgcggtcttg aagggatata ttgactttga atcagagcca
      481 tggcccgtaa tatctgacag tgcaaaagac ctaatccgga atatgctatg ttctaggcct
      541 tcagaccgtt tgactgctca tgaagtgcta tgccaccctt ggatttgcga aaatggggtt
      601 gctcctgaca gagccctaga tccagccgta ctttctcgtc tcaaaacatt ttctgctatg
      661 aacaagttaa agaagatggc cttacgggtg atagctgaaa gcctatctga ggaggagatt
      721 gctgggctca gagagatgtt ccaggcaatg gatactgata atagtggtgc aatcacattt
      781 gacgaactta aagctggttt gcggagatac ggctctacca tgaaggatac agagatacgt
      841 gatcttatgg atgcggctga tgtggacaac agtggaacaa ttgattatgg tgaatttata
      901 gctgctacag ttcatcttaa caaactagaa cgtgaagaac atctgattgc agccttccaa
      961 tactttgata aggatggaag tggttatatt acagttgatg agctccagca agcttgtaca
     1021 gaacataaca tgactgacgt ccttcttgat gatattataa aagaagttga tcaggataat
     1081 gatggaagga ttgactacgg tgagtttgtc gccatgatgc aaaagggcaa tgtgaaagga
     1141 aacatgccgg ttggaagacg gactatgagg aatagtctaa atgtgagcat gagagatgca
     1201 ccaggagctc atgacccagc tttcttgtac aaagttggca ttataagaaa gcattgctta
     1261 tcaatttgtt gcaacgaaca ggtcactatc agtcaaaata aaatcattat ttgccatcca
     1321 gctgcagctc tggcccgtgt ctcaaaatct ctgatgttac attgcacaag ataaaaatat
     1381 atcatcatga acaataaaac tgtctgctta cataaacagt aatacaaggg gtgttatgag
     1441 ccatattcaa cgggaaacgt cgaggccgcg attaaattcc aacatggatg ctgatttata
     1501 tgggtataaa tgggctcgcg ataatgtcgg gcaatcaggt gcgacaatct atcgcttgta
     1561 tgggaagccc gatgcgccag agttgtttct gaaacatggc aaaggtagcg ttgccaatga
     1621 tgttacagat gagatggtca gactaaactg gctgacggaa tttatgcctc ttccgaccat
     1681 caagcatttt atccgtactc ctgatgatgc atggttactc accactgcga tccccggaaa
     1741 aacagcattc caggtattag aagaatatcc tgattcaggt gaaaatattg ttgatgcgct
     1801 ggcagtgttc ctgcgccggt tgcattcgat tcctgtttgt aattgtcctt ttaacagcga
     1861 tcgcgtattt cgtctcgctc aggcgcaatc acgaatgaat aacggtttgg ttgatgcgag
     1921 tgattttgat gacgagcgta atggctggcc tgttgaacaa gtctggaaag aaatgcataa
     1981 acttttgcca ttctcaccgg attcagtcgt cactcatggt gatttctcac ttgataacct
     2041 tatttttgac gaggggaaat taggccggga agccgatctc ggcttgaacg aattgttagg
     2101 tggcggtact tgggtcgata tcaaagtgca tcacttcttc ccgtatgccc aactttgtat
     2161 agagagccac tgcgggatcg tcaccgtaat ctgcttgcac gtagatcaca taagcaccaa
     2221 gcgcgttggc ctcatgcttg aggagattga tgagcgcggt ggcaatgccc tgcctccggt
     2281 gctcgccgga gactgcgaga tcatagatat agatctcact acgcggctgc tcaaacttgg
     2341 gcagaacgta agccgcgaga gcgccaacaa ccgcttcttg gtcgaaggca gcaagcgcga
     2401 tgaatgtctt actacggagc aagttcccga ggtaatcgga gtccggctga tgttgggagt
     2461 aggtggctac gtctccgaac tcacgaccga aaagatcaag agcagcccgc atggatttga
     2521 cttggtcagg gccgagccta catgtgcgaa tgatgcccat acttgagcca cctaactttg
     2581 ttttagggcg actgccctgc tgcgtaacat cgttgctgct gcgtaacatc gttgctgctc
     2641 cataacatca aacatcgacc cacggcgtaa cgcgcttgct gcttggatgc ccgaggcata
     2701 gactgtacaa aaaaacagtc ataacaagcc atgaaaaccg ccactgcgcc gttaccaccg
     2761 ctgcgttcgg tcaaggttct ggaccagttg cgtgagcgca tacgctactt gcattacagt
     2821 ttacgaaccg aacaggctta tgtcaactgg gttcgtgcct tcatccgttt ccacggtgtg
     2881 cgtcacccgg caaccttggg cagcagcgaa gtcgaggcat ttctgtcctg gctggcgaac
     2941 gagcgcaagg tttcggtctc cacgcatcgt caggcattgg cggccttgct gttcttctac
     3001 ggcaaggtgc tgtgcacgga tctgccctgg cttcaggaga tcggaagacc tcggccgtcg
     3061 cggcgcttgc cggtggtgct gaccccggat gaagtggttc gcatcctcgg ttttctggaa
     3121 ggcgagcatc gtttgttcgc ccaggactct agctatagtt ctagtggttg gctactaata
     3181 ggttgtattg atgttggacg agtcggaatc gcagaccgat accaggatct tgccatccta
     3241 tggaactgcc tcggtgagtt ttctccttca ttacagaaac ggctttttca aaaatatggt
     3301 attgataatc ctgatatgaa taaattgcag tttcatttga tgctcgatga gtttttctaa
     3361 tcagaattgg ttaattggtt gtaacactgg cagagcatta cgctgacttg acgggacggc
     3421 gcaagctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     3481 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     3541 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     3601 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
     3661 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     3721 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     3781 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     3841 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     3901 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     3961 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     4021 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     4081 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     4141 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcta
     4201 gccaggaaga gtttgtagaa acgcaaaaag gccatccgtc aggatggcct tctgcttagt
     4261 ttgatgcctg gcagtttatg gcgggcgtcc tgcccgccac cctccgggcc gttgcttcac
     4321 aacgttcaaa tccgctcccg gcggatttgt cctactcagg agagcgttca ccgacaaaca
     4381 acagataaaa cgaaaggccc agtcttccga ctgagccttt cgttttattt gatgcctggc
     4441 agttccctac tctcgc
//
