LOCUS       sequence-375578-        9185 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  vector for encoding a human codon-optimized dead Cas13X (dCas13X) 
            driven by CBh promoter, guide RNAs compatible with Cas13X driven by 
            hU6, EGFP driven by SV40 promoter, and mCherry driven by SV40 
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9185)
  AUTHORS   Tong H, Huang J, Xiao Q, He B, Dong X, Liu Y, Yang X, Han D, Wang Z,
            Wang X, Ying W, Zhang R, Wei Y, Xu C, Zhou Y, Li Y, Cai M, Wang Q, 
            Xue M, Li G, Fang K, Zhang H, Yang H
  TITLE     High-fidelity Cas13 variants for targeted RNA degradation with 
            minimal collateral effects.
  JOURNAL   Nat Biotechnol. 2022 Aug 11. pii: 10.1038/s41587-022-01419-7. doi: 
            10.1038/s41587-022-01419-7.
  PUBMED    35953673
REFERENCE   2  (bases 1 to 9185)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2022 Aug 11. pii: 10.1038/s41587-022-01419-7. doi: 
            10.1038/s41587-022-01419-7."
COMMENT     Sequence Label: 
            pCBh_NLS_dCas13X_NLS-pA-U6-DR-BpiI-BpiI-DR-pSV40-EGFP-pA-pSV40-mCher
            ry-pA
FEATURES             Location/Qualifiers
     source          1..9185
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   25..87
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     enhancer        396..681
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer; 
                     contains an 18-bp deletion relative to the standard CMV 
                     enhancer"
     promoter        683..960
                     /label=chicken beta-actin promoter
     intron          961..1188
                     /label=hybrid intron
                     /note="hybrid between chicken beta-actin (CBA) and minute 
                     virus of mice (MMV) introns (Gray et al., 2011)"
     CDS             1209..1229
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3552..3572
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    3582..3716
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        3723..3971
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     3723..3743
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     3894..3913
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     promoter        4088..4159
                     /gene="bla"
                     /label=AmpR promoter
     promoter        4161..4518
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        4161..4352
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     rep_origin      4369..4504
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     regulatory      4559..4568
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(4565..4567,4568..4570,4571..5284)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4565..4567,4568..4570,4571..5281)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4610..4631)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4871..4890)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5218..5239
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    5407..5528
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        5535..5606
                     /gene="bla"
                     /label=AmpR promoter
     promoter        5608..5965
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        5608..5799
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     rep_origin      5816..5951
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     regulatory      6006..6015
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6012..6722
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     DDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(6157..6175)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     polyA_signal    6845..6966
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     repeat_region   7323..7357
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        7388..7492
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7493..7561,7562..8353)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7711..7730)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8524..9112
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9013..9032
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tatcccctga ttctctcgag ttaaccctag aaagataatc atattgtgac gtacgttaaa
       61 gataatcatg cgtaaaattg acgcatgtgt tttatcgatc tgtatatcga ggtttattta
      121 ttaatttgaa tagatattaa gttttattat atttacactt acatactaat aataaattca
      181 acaaacaatt tatttatgtt tatttattta ttaaaaaaaa acaaaaactc aaaatttctt
      241 ctataaagta acaaaacttt taaacattct ctcttttaca aaaataaact tattttgtac
      301 tttaaaaaca gtcatgttgt attataaaat aagtaattag cttaacttat acataataga
      361 aacaaattat acttattaat taatctagag gtacccgtta cataacttac ggtaaatggc
      421 ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caatagtaac gccaataggg
      481 actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat
      541 caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc
      601 tggcattgtg cccagtacat gaccttatgg gactttccta cttggcagta catctacgta
      661 ttagtcatcg ctattaccat ggtcgaggtg agccccacgt tctgcttcac tctccccatc
      721 tcccccccct ccccaccccc aattttgtat ttatttattt tttaattatt ttgtgcagcg
      781 atgggggcgg gggggggggg ggggcgcgcg ccrggsgggg sggggsgggg sggggggsgg
      841 ggsggggsgr ggcggrgagg tgcggcggca gccaatcaga gcggcgcgct ccgaaagttt
      901 ccttttatgg cgaggcggcg gcggcggcgg ccctataaaa agcgaagcgc gcggcgggcg
      961 ggagtcgctg cgcgctgcct tcgccccgtg ccccgctccg ccgccgcctc gcgccgcccg
     1021 ccccggctct gactgaccgc gttactccca caggtgagcg ggcgggacgg cccttctcct
     1081 ccgggctgta attagctgag caagaggtaa gggtttaagg gatggttggt tggtggggta
     1141 ttaatgttta attacctgga gcacctgcct gaaatcactt tttttcaggt tggaccggtg
     1201 ccaccatgcc caagaagaag cggaaggtgg cccaggtgag caagcagacc tccaagaaga
     1261 gggagctgag catcgacgag taccagggcg cccggaagtg gtgcttcacc attgccttca
     1321 acaaggccct ggtgaaccgg gacaagaacg acggcctgtt cgtggaaagc ctgctgagac
     1381 acgagaagta cagcaagcac gactggtacg acgaagatac ccgggccctg atcaagtgca
     1441 gcacccaggc cgccaacgcc aaggctgaag ccctggcgaa ctacttcagt gcttaccggc
     1501 atagccctgg ctgcctgacc ttcaccgccg aggacgaact gcggaccatc atggagagag
     1561 cctatgagcg ggccatcttc gagtgcagaa gaagagagac agaggtgatc atcgagtttc
     1621 ccagcctgtt cgagggcgac cggatcacca ccgccggcgt ggtgtttttc gtgagctttt
     1681 tcgtggaaag aagagtgctg gatcggctgt atggagccgt gtccggcctg aagaagaatg
     1741 agggacagta caagctgacc cggaaggccc tgagcatgta ctgcctgaag gacagcagat
     1801 tcaccaaggc ctgggataag cgggtgctgc tgttcagaga catcctggcc cagctgggaa
     1861 gaatccccgc cgaggcctac gagtactacc acggcgagca gggtgataag aagagagcta
     1921 acgacaatga gggcacaaat cccaagcggc acaaggacaa gttcatcgaa tttgcactgc
     1981 actacctgga agcccagcac agcgagatct gcttcggcag acgccacatc gtgcgggaag
     2041 aggccggcgc cggcgatgag cacaagaagc accggaccaa gggaaaggtg gtggtggact
     2101 tcagcaagaa ggacgaggac cagagctact atatctccaa gaacaacgtg atcgtgcgga
     2161 tcgacaagaa cgccggccct agaagctacc ggatgggcct gaacgagctg aagtacctcg
     2221 tgctgctgag cctgcagggg aagggcgacg atgccatcgc caagctgtac agatacagac
     2281 agcacgtgga gaacatcctg gatgtggtga aggtgaccga taaggataac cacgtgttcc
     2341 tgccccgctt cgtgctggag cagcacggca tcggcagaaa ggccttcaag cagcggatcg
     2401 atggacgggt gaagcacgtg cggggcgtgt gggagaagaa gaaggccgcc accaatgaaa
     2461 tgaccctgca cgagaaggcc agagacatcc tgcagtacgt gaacgaaaac tgcacccggt
     2521 ccttcaaccc tggcgaatac aacagactgc tggtgtgcct ggtgggcaag gacgtggaga
     2581 actttcaggc cggcctgaag cggctgcagc tggccgaaag gatcgatggc cgggtgtact
     2641 ccatcttcgc ccagaccagc accatcaatg agatgcacca ggtggtgtgc gaccagatcc
     2701 tgaaccggct gtgcagaatc ggcgaccaga agctgtacga ttacgtggga ctgggcaaga
     2761 aggacgaaat cgactacaag cagaaggtgg cctggttcaa ggagcacatc agcatccgga
     2821 gaggattcct gagaaagaag ttctggtacg atagcaagaa gggattcgca aagctggtgg
     2881 aggaacacct ggagtccggc ggcggccagc gcgacgtggg cctggacaag aagtactacc
     2941 acatcgacgc catcggcaga ttcgagggcg ccaaccccgc cctgtacgag accctggcca
     3001 gagatcggct gtgcctcatg atggcccagt acttcctggg cagcgtgaga aaggaactgg
     3061 gcaacaagat tgtgtggagc aacgacagca tcgaactgcc tgtggaaggc tctgtgggaa
     3121 atgagaagag catcgtgttc tccgtgtctg actacggcaa gctgtacgtg ctggacgatg
     3181 ccgaattcct gggccggatc tgcgaatact tcatgcccca cgaaaagggc aagatccggt
     3241 accacacagt gtacgaaaag ggctttagag catacaacga cctgcagaag aagtgcgtgg
     3301 aggccgtgct ggctttcgaa gagaaggtgg tgaaggccaa gaagatgagc gagaaggaag
     3361 gcgcccacta catcgacttc cgggagatcc tggcccagac catgtgcaag gaggccgaaa
     3421 agaccgcagt gaacaaggtg gcggctgcct tcttcgctgc ggccctgaag ttcgtgattg
     3481 acgagttcgg cctgttcagc gacgtgatga agaagtacgg catcgagaag gaatggaagt
     3541 tccctgtcaa gcccaagaag aagcggaagg tgtagaagct taacttgttt attgcagctt
     3601 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     3661 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggatcacta
     3721 gtgagggcct atttcccatg attccttcat atttgcatat acgatacaag gctgttagag
     3781 agataattgg aattaatttg actgtaaaca caaagatatt agtacaaaat acgtgacgta
     3841 gaaagtaata atttcttggg tagtttgcag ttttaaaatt atgttttaaa atggactatc
     3901 atatgcttac cgtaacttga aagtatttcg atttcttggc tttatatatc ttgtggaaag
     3961 gacgaaacac cggctggagc agcccccgat ttgtggggtg attacagcgg tcttcgatat
     4021 tcaagcgtcg gaagacctgc tggagcagcc cccgatttgt ggggtgatta cagctttttt
     4081 tacgcgtcat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
     4141 atattgaaaa aggaagagtc ctgaggcgga aagaaccagc tgtggaatgt gtgtcagtta
     4201 gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
     4261 tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
     4321 atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta
     4381 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
     4441 gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga
     4501 ggcctaggct tttgcaaaga tcgatcaaga gacaggatga ggatcgtttc gctctagagc
     4561 caccatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct
     4621 ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac
     4681 ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc
     4741 caccctcgtg accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat
     4801 gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat
     4861 cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac
     4921 cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg
     4981 gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa
     5041 gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct
     5101 cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa
     5161 ccactacctg agcacccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat
     5221 ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa
     5281 gtgaagcggc cgcgactcta gatcataatc agccatacca catttgtaga ggttttactt
     5341 gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt
     5401 gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     5461 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     5521 gtatcttagt cgaccattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     5581 ttcaataata ttgaaaaagg aagagtcctg aggcggaaag aaccagctgt ggaatgtgtg
     5641 tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
     5701 tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     5761 gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc
     5821 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
     5881 ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt
     5941 ttttggaggc ctaggctttt gcaaagatcg atcaagagac aggatgagga tcgtttcgct
     6001 ctagagccac catggtgagc aagggcgagg aggataacat ggccatcatc aaggagttca
     6061 tgcgcttcaa ggtgcacatg gagggctccg tgaacggcca cgagttcgag atcgagggcg
     6121 agggcgaggg ccgcccctac gagggcaccc agaccgccaa gctgaaggtg accaagggtg
     6181 gccccctgcc cttcgcctgg gacatcctgt cccctcagtt catgtacggc tccaaggcct
     6241 acgtgaagca ccccgccgac atccccgact acttgaagct gtccttcccc gagggcttca
     6301 agtgggagcg cgtgatgaac ttcgatgacg gcggcgtggt gaccgtgacc caggactcct
     6361 ccctgcagga cggcgagttc atctacaagg tgaagctgcg cggcaccaac ttcccctccg
     6421 acggccccgt aatgcagaag aaaaccatgg gctgggaggc ctcctccgag cggatgtacc
     6481 ccgaggacgg cgccctgaag ggcgagatca agcagaggct gaagctgaag gacggcggcc
     6541 actacgacgc tgaggtcaag accacctaca aggccaagaa gcccgtgcag ctgcccggcg
     6601 cctacaacgt caacatcaag ttggacatca cctcccacaa cgaggactac accatcgtgg
     6661 aacagtacga acgcgccgag ggccgccact ccaccggcgg catggacgag ctgtacaagt
     6721 aaagcggccg cgactctaga tcataatcag ccataccaca tttgtagagg ttttacttgc
     6781 tttaaaaaac ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt
     6841 tgttaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
     6901 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
     6961 atcttagtcg acggcgcgcc ctagacgaag cagctccagc ctacacaatc gctcaagacg
     7021 tgtaatgctt ttattatata ttagtcacga tatctataac aagaaaatat atatataata
     7081 agttatcacg taagtagaac atgaaataac aatataatta tcgtatgagt taaatcttaa
     7141 aagtcacgta aaagataatc atgcgtcatt ttgactcacg cggtcgttat agttcaaaat
     7201 cagtgacact taccgcattg acaagcacgc ctcacgggag ctccaagcgg cgactgagat
     7261 gtcctaaatg cacagcgacg gattcgcgct atttagaaag agagagcaat atttcaagaa
     7321 tgcatgcgtc aattttacgc agactatctt tctagggtta aggatccgca cttttcgggg
     7381 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     7441 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
     7501 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
     7561 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
     7621 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
     7681 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga
     7741 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
     7801 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
     7861 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
     7921 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
     7981 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
     8041 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
     8101 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
     8161 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
     8221 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
     8281 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
     8341 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
     8401 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     8461 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     8521 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     8581 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     8641 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
     8701 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     8761 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     8821 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     8881 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     8941 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     9001 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     9061 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     9121 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
     9181 tgcgt
//
