LOCUS       sequence-375582-       22894 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  An E. coli cloning vector for the integration of heterologous genes 
            into a linear plasmid pSA3239 in Streptomyces lavendulae subsp. 
            lavendulae CCM 3239 through homologous recombination.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 22894)
  AUTHORS   Novakova R, Homerova D, Csolleiova D, Rezuchova B, Sevcikova B, 
            Javorova R, Feckova L, Kormanec J
  TITLE     A stable vector for efficient production of heterologous proteins 
            and secondary metabolites in streptomycetes.
  JOURNAL   Appl Microbiol Biotechnol. 2022 Nov;106(21):7285-7299. doi: 
            10.1007/s00253-022-12187-4. Epub 2022 Sep 29.
  PUBMED    36173451
REFERENCE   2  (bases 1 to 22894)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1007/s00253-022-12187-4"; journalName: "Appl Microbiol 
            Biotechnol"; date: "2022-11"; volume: "106"; issue: "21"; pages: 
            "7285-7299"
COMMENT     Sequence Label: pCosSAD1AprR
FEATURES             Location/Qualifiers
     source          1..22894
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(4893..5693)
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRAALGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPHSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     oriT            6009..6118
                     /note="incP origin of transfer"
     protein_bind    complement(6125..6158)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     primer_bind     complement(11897..11917)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(11897..11915)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(11944..11962)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        12030..12134
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(12135..12203,12204..12995)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(12353..12372)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      13166..13754
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     13655..13674
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     13908..13925
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    14139..14273
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(14163..14182)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     14217..14236
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(14698..14718)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          14848..14913
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             complement(15333..16127)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(15444..15463)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     16054..16073
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(16484..16813)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      16498..16633
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     16798..16818
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     primer_bind     18723..18742
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        18723..18741
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 tgaccgggac gccgttctcc gggtgcgggc acaggatgat cgtgccggtc cactgcgcgc
       61 gcagggcggc gatgtggtcg cggttgccgc cctccaggat gtgcaggaag gccaccggcg
      121 ggagggccgc gacgagcgcc tcgtagaggg cgtgggtgtc gccctcgacg atgtcgttgt
      181 acgggttcga cggcgagatg cgcacgccga cgcggtcggc gccgatgcgc tcggagaccg
      241 cctcgatcac ctcgatcgcg aagcggacgc ggttctcgac cgaaccgccg tactggtcgg
      301 tgcgctggtt gctgttctcc gccaggaact ggtggagcag gaagccgttg cccgcgtgga
      361 tctcgacgcc gtcgaagccg gcctcgatgg cgagctcggc ggagtccgcg tgctccttga
      421 cgacctcggc gatctcctcg accgtcaggg cgcgcggctc ggggtagtcg agcgggccct
      481 cggggccgta ggcctggccg gccgcggcga cggcggacgg ggcgaccgac tggtggccgc
      541 tcgggtacag cgaggggtgg ccgatgcggc cggcgtgcat gagctgcacc acgatcttgc
      601 cgcccgcggc gtgcacggcc tcggtgacgg gcttccagga ctcggcctgc tcccgggtgt
      661 gcagacccgg gctgttgccg aagccctggc ccacggcgct gggctgcgcg ccctcggtga
      721 tgatcaggcc ggcgccggcc cgctggccgt agtaggtggc catcaggtcg gtggccagac
      781 cctcggcggt ggcccggttg cgggacatgg gcgccatgac cactcggttg ctcagggcca
      841 gcttccccag ggtgaccggc tcgaacagct tctcggacat gtgtctctcc agactgaggt
      901 caacgcgtgg tgcggtggtg gaacggaaaa cgcccgtggg cgggacggat cagcggcccg
      961 cgagttcggc gaggtgacgg gagtgggcga cggccagccg gccggcggcc tcggcgcagg
     1021 aggcgaggac ggccatgttg gccttggccg agcggccctc ggtcgccgcg tcgacggcgc
     1081 gcatcaggta cttggtgatg gcctggcccg acaccccgtc acgggtggcc tcggcgaccg
     1141 cggcgcggat ggccgtgtcc acctcggtgg agtcgatggc gtcctcctcc ctgaccgggg
     1201 tggtgatcag gaaggagctg ttgttgccga gggcccagtg ggcctcgacg gcgcgggcga
     1261 tgacctgctc gtcgtccagg cggtggggcg ccttgtggcc gctcgtacgg cagtagaagg
     1321 cggggaagtc gtccgagcgg taggcgacga cggggacgca ctgcgtctcc aggtactcca
     1381 gggtcagacc gaggtcgagg atcatcttcg cgcccgcgca gacgacggcg accttggagc
     1441 gggtgaactg gatgaggtcc gaggacacgt cccaggtctc ctgggcgccc cggtgcacgc
     1501 cgccgatgcc ggcggacgag aagaacggga tccccgcgag ctcggcggcg acgacggagg
     1561 tggccacggt ggtggcaccg aggccgccct gcgccagcac gaccggcagg tcccggctgg
     1621 acaccttggg gatgcccggc agggacgcga agcgctcgat gtcggagtcg ctcatgccga
     1681 cccggatctc accggcctcg atgcagatcg tcgcgggcac ggcgccaccg gcccgtacgg
     1741 cgtcctccac cttgcgggcg gtggccgcgt tgtccgggta gggcaggccg tgcgtgatga
     1801 cgttgctctc cagggcgacg acggccgcgc cctcgtggag ggcctcgcgg acctcctcgg
     1861 tgtagacgac ggggatgccg ttgcttgcgc tcatcggggc tctctcaact ctcgttccag
     1921 tagccgggcg ctttgatcag ctcgtcccgg cgggccgcta tctcggcgag gacctggctg
     1981 tcccagtcct ccggtgtgac cggctcaagg gagatcgaga cggtgtcctc cccggtggcg
     2041 aaggtgctcg tcacagcggc ggtgaccgct tcgtcgatgg ccctgagctg ctcttccgtg
     2101 aagttcctcg ggtagtgccg gacgtggatg tggggcacgg tctcggtcct ctcacggagt
     2161 ggtgcgcgtt ctggtggctc ggacgtgcgt gtctgtgggg gggtgggcgg cacaccgcgg
     2221 acgggggaga ccgcggtgtg ccaccaccgc tccccgcccg cgcccagggg ggaaggggcc
     2281 cggcggcggg gagtctcggg cgggggccgt cggggcgtta cgcctcgacg gattcccgct
     2341 gggcagggcg ggccggctcg ggccgcggct gcttgcggcc cggccgcggc tgggccagga
     2401 agaccgcgcc gacgacgatc agcgctccga cgatctggag cggcgagagc gactggtcga
     2461 ggacgaagta gccgagcagg gtggccgcca ggggggaggc gaagctcagg aacgagacgg
     2521 ccagggcggg caggcgctgc actccgcgga accagatcgc gtaggcgaac agggccccga
     2581 tgagaccgag gtagaggaag ccccagacgt tcttgccggt gatctcgtcg ggcaggccct
     2641 cgccgatcag cgcgatcggg agcaggacca gaccgccgac ggtcagctgc cagcccgtga
     2701 agggcagcag gccgacgccc tcggggcggc cccagcgctt ggtcagcacg atgccggagg
     2761 ccatgctgag cgcgcccagc aggccggcga tgacaccggt ggcgttcagc ccggcgttcg
     2821 gctggaggac gagcagcgcc acgccggccg cgccgagcgc gcaggcgccc aggtggatcc
     2881 gccggatctt ctccttcagc aggaccgctc cgagcagcag cacgatcatc ggctggatgg
     2941 acatcaccag cgcggcgaca ccgcccggca ggtggtaggc ggccacgaag agcaggtaga
     3001 agaaggcgcc gatgttcagg acgcccagga ccagggcccg ccaccaccag atccccttcg
     3061 gcagcgtacg gccgaagagc accaggatca gaccggcggg cagggcgcgg agcaccgccg
     3121 ccagcagggg gcgtccctcg ggcagcagtt ccgtggtgac caggtacgtc gaaccccaga
     3181 tcgccggggc caacgcggtg agcgcggagt ccgcgagggt gcggctgctc ttcatcgggt
     3241 ggctcccgag tcggtcgtgc cgaagaagcg gtcaccgaag tcgccgatgc ccgggatcat
     3301 gaacgcgtgc tcgttgaggc gctcctcgac cgaggaggtg acgatcttga tggccgggtg
     3361 ctcgcggtgg accgcggcga tgccctcggg tgcggcgagg aagttgacga agacgatgtt
     3421 ctcctcgcgg acgcccgcgt cgagcagcac gccgatggcg gcgttggcgc tgccgccggt
     3481 ggcgagcatc ggctccagga gcaggacgtg ccggtccgcg atgtcggccg gcaggttctg
     3541 gtagatcagg tgcggaagct tggtggtctt gttccgctgg atgaggacct tgccgacgcg
     3601 gatgttcggc acgacctcgc gcagctcgtc caccatggag tcaccggccc ggatcacggg
     3661 cacggcgcac agcttcggga cgaacttgag tccttcgtac gtctcgccga ccggcgtgac
     3721 gaccttctgc ttgtcgaacg gcagctggtc gagggccgat tcgagcagga ggcggatgat
     3781 gcgccgggag tagaacacga agtccgcacg cgacgcgtcg cggtcccgga tgatcgtgtg
     3841 catggcccgg agctggttgg tctgcggcag cgggtggacg ttgtggtggc cggtcgccat
     3901 gagaacctgc ttctcgtcgg tcggtggaga cgggctgcgg ccgggcccgg ggcccagggc
     3961 cccgggcggc cgcaaccgca tggtgaggta ccgaggtcag gagcccgcga tctcggcgaa
     4021 cgccttgcgc gactcggcca cgaacttcgc gaccttggcg cggtccttgc ggcggtcgtc
     4081 gccctccagg aggctgtggg cgtcgacggc ggcgggcttg acgaagcgga tcgcgtcggc
     4141 cacgttctcc gggctcaggc cgccggccat catcaggggc ttgggggaac ggcgcacgag
     4201 ctcggcgctg acgttccagt cgtgggtgag gccggtggcg cccttggcac cggtggccgg
     4261 gttgaaggtg tcggtgatga acatgtccac cacgtccgcg acctcgtcga cgagcttgag
     4321 cagctcctcg gcgttgtcct ccttcacgac caggctcttg agcacgtaga ggtcggggcg
     4381 gttggccttg agcttgcgca gttgctccgg ctcgacgtcg ccgtggagct gaacggcctt
     4441 gaccccgagc tgggtggtga acgcgctgat ctcgtcggca tccgtcatgt agctgatgag
     4501 gacgccctcc tgaggtgcct tcaggccggc gatgatctcc gtggccgcgg cctcggagat
     4561 gtcgtccttg cccgacggga ggcggagcgg gaagccgaac cagtcgacgc cctcctcggc
     4621 cagcagagcg gcctcctcgg cgtcgatgat gccggcgacc tggaccaggt ccttcacgtc
     4681 actcatgtgt gttccttttc gggatgaatc ttatgaatca aagcgagtta gggcagaacg
     4741 aggtgacgtg ccccggtatg acggattcgt tacacgtctc gggatgctca cgaaaagcct
     4801 cattgatacc gatccaaggg tcaacgggct ttatgtcaga tccgcgtcaa ccttcttcag
     4861 gtatcggtca ggaataggaa cttatgagct cagccaatcg actggcgagc ggcatcgcat
     4921 tcttcgcatc ccgcctctgg cggatgcagg aagatcaacg gatctcggcc cagttgaccc
     4981 agggctgtcg ccacaatgtc gcgggagcgg atcaaccgag caaaggcatg accgactgga
     5041 ccttccttct gaaggctctt ctccttgagc cacctgtccg ccaaggcaaa gcgctcacag
     5101 cagtggtcat tctcgagata atcgacgcgt accaacttgc catcctgaag aatggtgcag
     5161 tgtctcggca ccccataggg aacctttgcc atcaactcgg caagatgcag cgtcgtgttg
     5221 gcatcgtgtc ccacgccgag gagaagtacc tgcccatcga gttcatggac acgggcgacc
     5281 gggcttgcag gcgagtgagg tggcaggggc aatggatcag agatgatctg ctctgcctgt
     5341 ggccccgctg ccgcaaaggc aaatggatgg gcgctgcgct ttacatttgg caggcgccag
     5401 aatgtgtcag agacaactcc aaggtccggt gtaacgggcg acgtggcagg atcgaacggc
     5461 tcgtcgtcca gacctgacca cgagggcatg acgagcgtcc ctcccggacc cagcgcagca
     5521 cgcagggcct cgatcagtcc aagtggccca tcttcgaggg gccggacgct acggaaggag
     5581 ctgtggacca gcagcacacc gccgggggta accccaaggt tgagaagctg accgatgagc
     5641 tcggcttttc gccattcgta ttgcacgaca ttgcactcca ccgctgatga catcagtcga
     5701 tcatagcacg atcaacggca ctgttgcaaa tagtcggtgg tgataaactt atcatcccct
     5761 tttgcttatg gagctgcaca tgaacccatt caaaggccgg cattttcagc gtgacatcat
     5821 tctgtgggcc gtacgctggt actgcaaata cggcatcagt taccgtgagc tgcattttcc
     5881 gctgcataac cctgcttcgg ggtcattata gcgatttttt cggtatatcc atcctttttc
     5941 gcacgatata caggattttg ccaaagggtt cgtgtagact ttccttggtg tatccaacgg
     6001 cgtcagccgg gcaggatagg tgaagtaggc ccacccgcga gcgggtgttc cttcttcact
     6061 gtcccttatt cgcacctggc ggtgctcaac gggaatcctg ctctgcgagg ctggcgggaa
     6121 cttcgaagtt cctatacttt ctagagaata ggaacttcga actgcaggtc gacggatccc
     6181 cggaatctta agggttaatt aaggactagt catggcttgc ctctctcctc gtccgccggg
     6241 gcatcggcgc acaccgggtc gcgacgagcc tggtcccacc ggatggagag tcgctcacgc
     6301 cgtcggccag ccgcgctgga gccttggcgt caggccgcgt agaggtcgaa ggtcgagctg
     6361 cggcgcgggc ccgcgaccac gtcgagcgcc gggtccaccc ggagcatcgc ctggtgcagg
     6421 cgctggagct gctgcgaggg ttccacgccc agttcgccga tcagccgggc ccggagcctg
     6481 cggtacacgt ccagcgcggt ggcctgccgt cccgaccggt acagcgcgac catcgcctgc
     6541 gagtgcaggc cctcgtgctg cgggttcctg gccatcagct cggtgagctc ggcgaggagt
     6601 tcgctgtgcc gtcccaggcg caggtccacg tcgatgcgcc gctcggtggt gaccagccgg
     6661 ctctccgcca gccgcagcac ctcgatctcc aggatcggcc cgaccttgac gtcgacgagg
     6721 gcgggcccct gccacaggtc gagggccttg cgcagcatgc tcgccgcgcg ttcgtcgtcg
     6781 ccctcgtcga aggcgatctg gccgccggcc accagggcct cgtacgcgtg gacgtccacc
     6841 gactcctgcg ggatctggag caggtagcct ccgtgccggg tcgcgagcac gtccttggcc
     6901 gtgccggggg cgcccggccc cagcgcggtg cccagtctgc ggcgcagctg caggatgtac
     6961 gtctgcaggg tggtgagggc gctctccggg agcttggtcc cccagatctc ctccatcagg
     7021 gtcggtacgg gcatcacccg gcccgggtag agcgcgagga gcgccaggat ctgacgcggc
     7081 ttgcccgccg taggaacgat cgatgccccg ttgacctcgg cactcaacgg acccagaacc
     7141 tgaatctcca cggtctcccg tctcccctcc gttatttcgt cgagatactc acggttttca
     7201 gtattcgacc cgatgacggt aaggcagccc agggcggcat caccgcgttc tcaagccgac
     7261 gtcgagcaga cctccgaagg gggcgaacgg ccgcgggatt cgaaaccccg ccttccgatt
     7321 gactgccaga acccctgttc agcccgtaga tcagttcggt cgccgctgtt ccgcttgcct
     7381 gatgcgcaaa ttcggtccac ggatttccgg gagataccgg aaagcagtgg gcgaatgatc
     7441 ttttaatgat ccgtcagccc actcgggcga gccgcttgcg ggccgtctcc ggggattccg
     7501 ccgggttctc cgggccgctc atcaggatcg accgctggag actgcgcagg gccgaggacg
     7561 gctcgatgcc gagctcgcgg acgagggtgg cgcgcagccg ctggtagacg ctgagcgcct
     7621 cgccgcggcg tccggagcgg tggagggcga gcatgaactg cccgtgcagg ttctcgtgga
     7681 tgcggtagcg gctggccagg acggtcagtt ccgcgagcag ttcgcggtgc cggcccaggc
     7741 gcaggtcggc ctcgatgcgc tggtcgagcg cgcacagccg ggtctcctcc aggcgccgcg
     7801 tctccatgtc cagctggaca ccgccctcca cgtcggcgaa ggccggcccg gaccagagcg
     7861 ccagcgcctc gcgcagcagc cgcgcggcac cggggaagtc gcccgcgtcc atcgcccggt
     7921 agcccattcc ggccagccgg tcgaattcgc ggacgtcgct ggtcccgccg gcgctgctga
     7981 gcatgtagcc gcccggcagc gtgacgagca cgtccttggg ggtgcgcgtc ccgtccgtgc
     8041 cctccagtgc ggtggcgatc agcccgcgca gctggagcac gtaggtctgc agggtcgtac
     8101 gggcactgcg cgggggctgc tcgccccaca gttcctcgat cagcgcggac accgggacca
     8161 cctggtccgc gtggagcgcg agcagggcca gcacctgacg gggcttggcg gcggtcggcg
     8221 tgatggagac cccgttctcc cgcacggtca gtgcgcccag tacatcgatg tccacgccgt
     8281 actcccctgc ttcgtccatg gatgagccct tgactttcgc tggaacgagc aaaaaacagc
     8341 cgggccggtt tgtcaatacc aaaccggctg taccgttttt cgccccccag tgaccgtgtc
     8401 gactcctgaa tctccccctg tgaactggga gtttcctgac cagaaggccg ccggcggccc
     8461 cggacgggcc cctccagggc cgcccgaggg cgggaagaac cgctctggaa ggggcgtcct
     8521 gcggccacta aacggccgag aaccgaccat gtggttggct cgcccgcccc ggcagcccct
     8581 ccgtacggac cgccctgccg ccaggttcag accgcggaag gccatccccg caccctcgga
     8641 cgtgatcaat acaaaccgct ttagcggttt gctcaccggc attcgcgctc ggcagtcgct
     8701 ggaacgacgc cggagccccg ccggaacacc gctcgaacca cggggccgcc ccctccgccg
     8761 acgggccgcc gacgggcgcc ctccgggccc cggacgcgcc gccgccaggc cggtccggcg
     8821 gcggggccgg cccgcaccgg gacgcctccc ccctaccgcc gggtggccgg atcggcggcg
     8881 ggcgggctcc ggcgcgcgcc cccgcggcgc aacggcatat gcagaaatct tcacgtaggc
     8941 aaacggtgaa cgagactggc gttacggtgc gccccgaccg caccgctccc ggcggccgca
     9001 ccccgccgcc cgtaccggcg gcacgccggc cccggaaccg gcgcaccacc cgtggactgc
     9061 ggggctccat gcgcggcggg acccccgggg cacccgcccc gaccccctgc cggccccacg
     9121 gggggccacg gccccgccgg gtgcggggag cccgcccgcg atccgcgcgc aaacccgggc
     9181 gcccgatgcg aatcggccat tcttcgtccc cgggggttca ccggacgtcc gacaaccggt
     9241 tcacgcaccc cgaggggtcc ccttgagatg cttcagtgcg agtggcggag atccgatgcc
     9301 ggatgagggg tgggggcacg gacggtaggg ggggtcaccg attgccggaa ccccaccctc
     9361 cgcatttttc atggccaagg gtcgtgatcc ctctggataa aaaaccggtt ggtatatatt
     9421 tttctcgcag tcacacctgt aactggccag cgctgcctag ggggaacaat gagcgcgagc
     9481 acgtttcaag tcgagcgcac agcggtctac gaaccggcgt ggcggacggc cgcccctccg
     9541 ctcggacaac ggggtaaccg cttcacctcg ctgacctcca ccgtcccgaa ggaattagtg
     9601 caccgcgcct cggtcgcaga agtccttctc accgactggg aacgcgtcga ccacagccgt
     9661 ttcaccgtca gcgcgcagtg gccccgctcc cacagcttct tcacgcccgt cgcgggcacc
     9721 taccacgatc cgctgatggt cgtcgagacg atccggcaga tcggcagcct gctgggacac
     9781 gccgagttcg gggtcccttt cggccagcac ttcctgctgt gggacctcag cgtcacggtc
     9841 gtcccggaac acctccaggt cagcaggacc cccgcctccc tcgacatcga cgtcagctgc
     9901 accgaggtca gacgccgcag cggtgacctg ggcggcctga actacgaggc cgtggtccac
     9961 cgcgccggcc acgtcgtcgc gacggggcgc gtctcctaca cctgcaccac acccgccgtg
    10021 taccggcgca tacgcccgcc gcacaccctg ctgcccggcc acaagccgct gcccctgacc
    10081 gctccggtgg caccgcagag cgtgggacgg gtctcgccca ccgacgtggt gctgtccccc
    10141 accggcacgc aggaccgctg gcagctgcgg gtggactccc tccatcccat cctcttcgac
    10201 cacccggtgg accacgttcc gggcatggtg ctgctggagg ccgcccggca ggccgcctcg
    10261 gccgccctgg gccgctcctc cctgctcccg ctcaacatct ccggcgattt caagcgctac
    10321 gccgaactcg acctgccgtg cttcatcgag gccgagcgcc tgcacgacgg cgcccccggc
    10381 ggggaggaga cggtggccgt gacgggccac caggacggcc acctcgtctt caactccacg
    10441 gtgacggcgg ccccttacat cggctgaccg aatgcccctc agcgcggccc gaaggccgtc
    10501 aggtgttccc cggcccactc ccggaacgaa cgggcgggcc tccccacgac gtccgcaacc
    10561 gtgctcccca caccggcctt ggccccggca cgctgccgct cggcgctctg cagcagtgcc
    10621 tccgccacgg cctccgggta ccgcgcaccc cacaccgcgc gggcctgctc cggatcccac
    10681 tcctcgaaac gcagtggacg gcccaacagg acggagagct gggccgtctg ctccaccgcc
    10741 gagagcgcct cgggtccggt cagcgtgtgc acgcgcccgg tgtgtcccgg ctcgccgaac
    10801 gcgcgcacgg ccacctccgc gatgtcgccc ggatcgacgc aggcgttcgg cgacgtcccg
    10861 tacaggccac gcacgacgcc ctccgcacgc accgcaccgg tccaggagag ggcgttggac
    10921 atgaaggaac gcgggcgcag cagcgtccac tccatccccg aaccccgcag ggcctcctcg
    10981 ttcccccgtt gccagcgggt gatcaggtca tcggccccgc cgtcctcgac ggcggccgcg
    11041 gaaagcttca ccacgtgccg caccccggcc gaccgggcgg cggccaggaa ccgctcgtcg
    11101 tcgtcgcggc cgacgcggct ggtcaccagg aaggcggtgt ccaccccccg cacggcccgg
    11161 gcgagcgaac cggggtcgtc gtagtccccg tggacggcct cgaccgtgcc ccggccggcc
    11221 acgacacgtt ccggttgcct cgccatggcc ctgaccgcgg ggcccgggcc gatgcgcccc
    11281 agcacttccc gtcccaccgt cccggtggcc cccgtcacga gaatcatggt gcttccttcc
    11341 ggtcatgtac gcgccctccg gcgcgtacgg ggttggcgta agagttaccc gctgtaagat
    11401 acgtacggga cggacggttt gcatcagggc ttccgacgct ccgagaatcc tgtgaaggaa
    11461 gggagccaca gtggctgagc aaattcgagc catccgcaca cgccaggcga tcctcggcgc
    11521 ggcggccaag atcttcgagg aacgcggcta ccaggccgcc accatctccg agatcctctc
    11581 ggaggcgggc gtgaccaagg gcgccctgta cttccacttc cagtcgaagg aggacctggc
    11641 cttcggcgtg ctcaacgcgc agaaccagag cctcgtcgtc cccgaccgcg cgatcaagat
    11701 gcaggggatc gtcgacatcg tcatgctgca cacctaccgc ctgcagacgg acccgatggt
    11761 ccgcgcgggc gtacggctga cgatggacca gctggcacag ggcctggacc gcagcggccc
    11821 cttccgcgcc tgggccgtgc cgatgcgcga gggcctggag cgggcccaga gccagggcga
    11881 gctgctcccc cagatccctt tagtgagggt taattgcggc cgcgaattct tgaagacgaa
    11941 agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga
    12001 cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
    12061 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
    12121 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
    12181 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
    12241 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
    12301 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
    12361 gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt
    12421 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
    12481 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
    12541 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
    12601 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
    12661 gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac
    12721 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
    12781 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
    12841 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
    12901 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
    12961 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
    13021 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
    13081 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
    13141 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
    13201 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
    13261 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag
    13321 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
    13381 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
    13441 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
    13501 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
    13561 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
    13621 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
    13681 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
    13741 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
    13801 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
    13861 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
    13921 gcgaggaagc ggaagagcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg
    13981 aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt
    14041 cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg
    14101 gtcctcaacg acaggagcac gatcatgcgc acccgtcaga tccagacatg ataagataca
    14161 ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa
    14221 tttgtgatgc tattgcttta tttgtaacca ttataagctg caataaacaa gttaacaaca
    14281 acaattgcat tcattttatg tttcaggttc agggggaggt gtgggaggtt ttttaaagca
    14341 agtaaaacct ctacaaatgt ggtatggctg attatgatct ctagtcaagg cactatacat
    14401 caaatattcc ttattaaccc ctttacaaat taaaaagcta aaggtacaca atttttgagc
    14461 atagttatta atagcagaca ctctatgcct gtgtggagta agaaaaaaca gtatgttatg
    14521 attataactg ttatgcctac ttataaaggt tacagaatat ttttccataa ttttcttgta
    14581 tagcagtgca gctttttcct ttgtggtgta aatagcaaag caagcaagag ttctattact
    14641 aaacacagca tgactcaaaa aacttagcaa ttctgaagga aagtccttgg ggtcttctac
    14701 ctttctcttc ttttttggag gagtagaatg ttgagagtca gcagtagcct catcatcact
    14761 agatggcatt tcttctgagc aaaacaggtt ttcctcatta aaggcattcc accactgctc
    14821 ccattcatca gttccatagg ttggaatcta aaatacacaa acaattagaa tcagtagttt
    14881 aacacattat acacttaaaa attttatatt taccttagag ctttaaatct ctgtaggtag
    14941 tttgtccaat tatgtcacac cacagaagta aggttccttc acaaagatcc ggaccaaagc
    15001 ggccatcgtg cctccccact cctgcagttc gggggcatgg atgcgcggat agccgctgct
    15061 ggtttcctgg atgccgacgg atttgcactg ccggtagaac tccgcgaggt cgtccagcct
    15121 caggcagcag ctgaaccaac tcgcgagggg atcgagcccg gggtgggcga agaactccag
    15181 catgagatcc ccgcgctgga ggatcatcca gccggcgtcc cggaaaacga ttccgaagcc
    15241 caacctttca tagaaggcgg cggtggaatc gaaatctcgt gatggcaggt tgggcgtcgc
    15301 ttggtcggtc atttcgaacc ccagagtccc gctcagaaga actcgtcaag aaggcgatag
    15361 aaggcgatgc gctgcgaatc gggagcggcg ataccgtaaa gcacgaggaa gcggtcagcc
    15421 cattcgccgc caagctcttc agcaatatca cgggtagcca acgctatgtc ctgatagcgg
    15481 tccgccacac ccagccggcc acagtcgatg aatccagaaa agcggccatt ttccaccatg
    15541 atattcggca agcaggcatc gccatgggtc acgacgagat cctcgccgtc gggcatgcgc
    15601 gccttgagcc tggcgaacag ttcggctggc gcgagcccct gatgctcttc gtccagatca
    15661 tcctgatcga caagaccggc ttccatccga gtacgtgctc gctcgatgcg atgtttcgct
    15721 tggtggtcga atgggcaggt agccggatca agcgtatgca gccgccgcat tgcatcagcc
    15781 atgatggata ctttctcggc aggagcaagg tgagatgaca ggagatcctg ccccggcact
    15841 tcgcccaata gcagccagtc ccttcccgct tcagtgacaa cgtcgagcac agctgcgcaa
    15901 ggaacgcccg tcgtggccag ccacgatagc cgcgctgcct cgtcctgcag ttcattcagg
    15961 gcaccggaca ggtcggtctt gacaaaaaga accgggcgcc cctgcgctga cagccggaac
    16021 acggcggcat cagagcagcc gattgtctgt tgtgcccagt catagccgaa tagcctctcc
    16081 acccaagcgg ccggagaacc tgcgtgcaat ccatcttgtt caatcatgcg aaacgatcct
    16141 catcctgtct cttgatcaga tcttgatccc ctgcgccatc agatccttgg cggcaagaaa
    16201 gccatccagt ttactttgca gggcttccca accttaccag agggcgcccc agctggcaat
    16261 tccggttcgc ttgctgtcca taaaaccgcc cagtctagct atcgccatgt aagcccactg
    16321 caagctacct gctttctctt tgcgcttgcg ttttcccttg tccagatagc ccagtagctg
    16381 acattcatcc ggggtcagca ccgtttctgc ggactggctt tctacgtgtt ccgcttcctt
    16441 tagcagccct tgcgccctga gtgcttgcgg cagcgtgaag ctttttgcaa aagcctaggc
    16501 ctccaaaaaa gcctcctcac tacttctgga atagctcaga ggccgaggcg gcctcggcct
    16561 ctgcataaat aaaaaaaatt agtcagccat ggggcggaga atgggcggaa ctgggcggag
    16621 ttaggggcgg gatgggcgga gttaggggcg ggactatggt tgctgactaa ttgagatgca
    16681 tgctttgcat acttctgcct gctggggagc ctggggactt tccacacctg gttgctgact
    16741 aattgagatg catgctttgc atacttctgc ctgctgggga gcctggggac tttccacacc
    16801 ctaactgaca cacattccac agccggatct gcaggaccca acgctgcccg agatgcgccg
    16861 cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc
    16921 attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc
    16981 gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac
    17041 gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg
    17101 ctcgccgagg cggcataaat cgccgtgacg atcagcggtc caatgatcga agttaggctg
    17161 gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac
    17221 agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg
    17281 aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc
    17341 atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag
    17401 gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg
    17461 ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg
    17521 agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac
    17581 cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgaggaac tttcggcggc
    17641 tttgctgtgc gacaggctca cgtctaaaag gaaataaatc atgggtcata aaattatcac
    17701 gttgtccggc gcggcgacgg atgttctgta tgcgctgttt ttccgtggcg cgttgctgtc
    17761 tggtgatctg ccttctaaat ctggcacagc cgaattgcgc gagcttggtt ttgctgaaac
    17821 cagacacaca gcaactgaat accagaaaga aaatcacttt acctttctga catcagaagg
    17881 gcagaaattt gccgttgaac acctggtcaa tacgcgtttt ggtgagcagc aatattgcgc
    17941 ttcgatgacg cttggcgttg agattgatac ctctgctgca caaaaggcaa tcgacgagct
    18001 ggaccagcgc attcgtgaca ccgtctcctt cgaacttatt cgcaatggag tgtcattcat
    18061 caaggacgcc gctatcgcaa atggtgctat ccacgcagcg gcaatcgaaa cacctcagcc
    18121 ggtgaccaat atctacaaca tcagccttgg tatccagcgt gatgagccag cgcagaacaa
    18181 ggtaaccgtc agtgccgata agttcaaagt taaacctggt gttgatacca acattgaaac
    18241 gttgatcgaa aacgcgctga aaaacgctgc tgaatgtgcg gcgctggatg tcacaaagca
    18301 aatggcagca gacaagaaag cgatggatga actggcttcc tatgtccgca cggccatcat
    18361 gatggaatgt ttccccggtg gtgttatctg gcagcagtgc cgtcgatagt atgcaattga
    18421 taattattat catttgcggg tcctttccgg cgatccgcct tgttacgggg cggcgacctc
    18481 gcgggttttc gctatttatg aaaattttcc ggtttaaggc gtttccgttc ttcttcgtca
    18541 taacttaatg tttttattta aaataccctc tgaaaagaaa ggaaacgaca ggtgctgaaa
    18601 gcgagctttt tggcctctgt cgtttccttt ctctgttttt gtccgtggaa tgaacaatgg
    18661 aagtcaacaa aaagcagagc ttatcgatga taagcggtca aacatgagaa ttcgcggccg
    18721 cataatacga ctcactatag ggatcatgta gtccggcagg aagctgacga ggtcgtcacg
    18781 gatcagctcc gccggaccgc gcatgtggac gctgtcctcg cgcatgccct cgtggccgag
    18841 ctgctcgtcg ctgacgcggc cgccgacgaa gaagtacgac ggaccgctct cgacgccgtt
    18901 cgcctgcagg acggagtcga tccggcggga ggccggcagc gggttgccgg tcttcgagct
    18961 gtagcccgag gacatgaagc cgaggccgag gccgttcatc atcaggtgct ggagcttctt
    19021 gccgagggtg acgtagtgca tccactcctc gggggcacgg ctgatcaccg agatgccgaa
    19081 gctggcggcc tggtacaccg actggttgat cgcgatgacc tgcttcttga ggacgatgtc
    19141 gtccgagaag cgggtgagcg tgccgtccgc gtagcggtac tggccctcgg ggaggttcgc
    19201 gaccttgctg ccgtgggtct ggacgtagac ctccgcgtgg tcctcgcgcc ggcccgtgtg
    19261 cgggaccagc tcgaaggtgc cgaggtagaa gtcctcctcg ggcacgtcca gcaggtcgcg
    19321 gacggccggc tcgtaggcca ggtcccggat gtcgaggccg tacgccggca gcacctgctc
    19381 gaagaggccg acgatgtggc cggtctcgat ctccaggacc tcctggatgt tgttcttgta
    19441 gaccggctcg atgccgccgc gcttgccgat gaagtggatg tgggcggtgg gccggccggt
    19501 ggcggccttc tcgctgatca ggaccagctg gtggcgctgc ggctggtagt agtagtagcc
    19561 cggctgcagc ccgccgacgc cctccagctc gaagtacagc tgcgtcgcgt acagcgcgcc
    19621 cggggaggcg tagccgtact tgggcagcag ccgctcctcg ctgaggtact ggccgaacca
    19681 gcgcaggatc tggcccagtt cggccggcgc caggtcggcg gccttgcgcg agtaggccgc
    19741 ggggacctgg ccgccgagga gcgccagcag gtcggcctcg gtgaccgcgc cgccctcgta
    19801 gaagcggtac gtcttgcggg cgaagacccg gctgcgctgc tcgggggtcg gctcggcgcc
    19861 cggcaggtcg taggcgacgc gggcggcgag gtcggcgtcg tcgcgcaggc ccgggttgga
    19921 cagctgcgcc ttgacctgga gcttgctctt cttcgaggcg tggtgggagc cgtggttgcc
    19981 ctggtccatc agggcggctt ccttctcgct cagctcgacg caggcgatga ggttctggaa
    20041 gccggtgcgg ccgtcgttct tgacgatgac ggcggcgttg cggacccagt cgtggttctc
    20101 gatcgccagg gatatctcgt ccagctcgac gcggtagccg cgcagcttga cctggttgtc
    20161 ggcgcggccg gcgaactgca cggtgccgtc cgggttccac tggcccaggt cgcccgtctt
    20221 gtagaggcgg accggaccgg cgccctcggt gacctcgatc tccaggaagc gctcggcggt
    20281 caggtcgggg cggtgcaggt agccgcgcgc cagctgaccg ccgccgatgt acagctcgcc
    20341 gatctcgccg acgccgacgg gcttgaggtc ctccttgccg aggatgtggt actcggtgtc
    20401 ggcaaccggg gcgccgatgg agatggactg cggcgcctcg cccagctcgg cggggtcgac
    20461 cgcgtacgag gaggagttga tggtgcactc ggtcggcccg tagacgttga tcagggcgcg
    20521 gccgggcatc tcctgcgtgg tctggatcgc caggaggcgc gagagggcct cgccaccgct
    20581 gaagatctgc tgcagggagg tgcactcggg gaacttctcg gtgtcgagca ggccctggag
    20641 cagggtgggg acgcactgca gggtggtcac gccgtacttg acgatggtct cgatgaggcc
    20701 ctcggggtcg gcgtagacgc cgggggcgcc catgacgacg gtggcaccgt tggccgggga
    20761 gaggatctcc cactgggcgg cgtcgaagct catcggggtc ttctgcagga tggtcttgct
    20821 gcggtcgatg ccgtaggtct cgcgcagcca gccgagctgg ctgacgatgc tgtggtgctc
    20881 gatcatcacg cccttcggct tgcccgtgct gccggaggtg tagatgacgt aggcgaggct
    20941 gtcggggcgg gcgccctcga tggccggggc cacctggccc tcgggcagga cgaacgcctc
    21001 ggactcgcgc agggtgacga ccctgacgtc ctgcggggcc agctcgcgca ggcgggtgac
    21061 caggcgctgc tgcgccagga tgatcttcgc ctggctgttc tcgatcatgt agcggagccg
    21121 gtcctcgggg tactccgggg acagcggcag gtaggcggcg ccggcggaca ggatgcccca
    21181 ggcgccgacc atcaggtcga tggacggctc gacgaagagg ccgacgcagt cgtccgtcga
    21241 gaccccgagg tgtctgaggt actcggcgag ggcggagctt ctggacgcca gctcgcggta
    21301 ggtgagcttc tcgtcccggt aggcgacggc ggtcgcctct gggtgctccg cgacccgctt
    21361 cgccagcagg tcgggcaacg tggtggtgcc gcgcagggtg ggctcaagca cgctggtctc
    21421 ctgaagagtc atttcccgct tccttacgtt ccgttgtgga gcaacgggcg cgtgcgaaac
    21481 gcccccgcac cgggccccgg tgtgtcggtg agcccgcgat gcgacacctc gcccgcgcgg
    21541 ggggcgcgtc cgcccggagg cggacacccg gcacggtggg cgtcggcggc cggagctgcg
    21601 tccggccggg aaaaccgaag aacgagggcg caaggccgga gcctcaccac ccgggatgac
    21661 cggaacggcg cggtgcgtgc ggccgccctc ccccaatgga cggacgggcc cccgtcggcc
    21721 cgtccgtccg ggcgttcacc ggctctggag gagccgggtc actgatccgt tctcgaagac
    21781 ctcgcgagag gctccggcaa tgagcggggc gatggacagg acggtgacct tgtccagtcc
    21841 gagcgccccg ggcaccggca gggtgtccgt gaagacgaac tcgctgacgc gcgagttctt
    21901 cagccggtcc gcggcggggc cggagagcac cccgtgcgtg gccgccacga tgacgtcctt
    21961 cgccccctgg gcgaagaggg cgtcggccgc ggcgcagatg gtgccgccgg tgtcgatcat
    22021 gtcgtcgacc aggacgcaca cgcggtcgcg cacgtcgccg accacctcgt gggccgtgac
    22081 ctggtgggcc acgtccttgt cgcgccgctt gtggacgatc gcgagcgggg cgcccagccg
    22141 gtcgcaccag ccgtccgcca cgcgcacgcg gcccgcgtcg ggcgagacca cggtcaggtc
    22201 ctcgcggtcc accttggagc cgatgtagtc cgcgaggacc ggcatggcga agaggtggtc
    22261 cacggggccg tcgaagaacc cctggatctg gtcggcgtgc aggtcaaccg tgaggatccg
    22321 gtccgcgccg gccgtcttga gcaggtccgc caccaggcgg gccgagatgg gctcccggcc
    22381 gctgtgcttg cggtcctggc gggcgtaccc gtagaacggc aggatcacgg tgatgctgcg
    22441 ggccgaggcc cgcttcagcg cgtcgatcat gatcagctgt tccatgatcg acttgttgat
    22501 cggcgccgtg tggctctgga ccacgaagca gtccgcgccg cgcacggagg cgtggtagcg
    22561 gatgtagatc tcgccgttgg cgaagtcgat cgccttcgtc gggaccagcg tcgtgcccag
    22621 ctcggcggcg atcgcctcgg ccagctcggg gtgtgcgcgc cccgagaaca gcagcatctg
    22681 cccggtctcg ctcacgcggc gccggcctcg gcgagggtcg ggtagtcggt gtaaccggtg
    22741 tggtcgccgc cgtagaactt gctctggtcg gcctccgcca gggcggcgcc ggtccggatg
    22801 cgctcggtca ggtccgggtt ggcgaggaac gccgagccga aggcgaccgc gcaggcgaca
    22861 ccggtctcca gcgcctcggt ggcgatctcg gcgt
//
