LOCUS       sequence-375607-        8789 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of a synthetic construct with 3' UTR of murine Bdnf in 
            the 3' UTR of an inactive BFP in mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8789)
  AUTHORS   Kaseniit KE, Katz N, Kolber NS, Call CC, Wengier DL, Cody WB, 
            Sattely ES, Gao XJ
  TITLE     Modular, programmable RNA sensing using ADAR editing in living 
            cells.
  JOURNAL   Nat Biotechnol. 2022 Oct 5. pii: 10.1038/s41587-022-01493-x. doi: 
            10.1038/s41587-022-01493-x.
  PUBMED    36198772
REFERENCE   2  (bases 1 to 8789)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2022 Oct 5. pii: 10.1038/s41587-022-01493-x. doi: 
            10.1038/s41587-022-01493-x."
COMMENT     Sequence Label: EK0307 CMV-TO-BFPmut-stop-3UTR(Bdnf) (FLP-IN)
FEATURES             Location/Qualifiers
     source          1..8789
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        4..383
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        384..587
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     538..558
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    589..607
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    610..628
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     638..662
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      754..763
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             760..1470
                     /codon_start=1
                     /product="enhanced monomeric blue fluorescent protein 
                     (Subach et al., 2011)"
                     /label=mTagBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRND
                     MALKLVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHAVAV
                     ARYCDLPSKLGHKLN"
     CDS             1489..1515
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             4391..4420
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(4476..4493)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4482..4706
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(4878..4897)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    5029..5076
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             5084..6106
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             5084..6106
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    6236..6357
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6273..6292)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6327..6346
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6406..6422)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6406..6422)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6419..6441)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6430..6446
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6454..6460,6461..6478,6479..6484))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6499..6520
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6637..6654)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6808..7396)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6888..6907)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7567..8358,8359..8427))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8190..8209
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8428..8532)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8607..8626)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gttgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
       61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      181 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
      241 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
      301 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
      361 tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat
      421 agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt
      481 tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc
      541 aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctctc cctatcagtg
      601 atagagatct ccctatcagt gatagagatc gtcgacgagc tcgtttagtg aaccgtcaga
      661 tcgcctggag acgccatcca cgctgttttg acctccatag aagacaccgg gaccgatcca
      721 gcctccggac tctagcgttt aaacttaagc ttcgccacca tggtgtctaa gggcgaagag
      781 ctgattaagg agaacatgca catgaagctg tacatggagg gcaccgtgga caaccatcac
      841 ttcaagtgca catccgaggg cgaaggcaag ccctacgagg gcacccagac catgagaatc
      901 aaggtggtcg agggcggccc tctccccttc gccttcgaca tcctggctac tagcttcctc
      961 tacggcagca agaccttcat caaccacacc cagggcatcc ccgacttctt caagcagtcc
     1021 ttccctgagg gcttcacatg ggagagagtc accacatacg aggacggggg cgtgctgacc
     1081 gctacccagg acaccagcct ccaggacggc tgcctcatct acaacgtcaa gatcagaggg
     1141 gtgaacttca catccaacgg ccctgtgatg cagaagaaaa cactcggctg ggaggccttc
     1201 accgaaacgc tgtaccccgc tgacggcggc ctggaaggca gaaacgacat ggccctgaag
     1261 ctcgtgggcg ggagccatct gatcgcaaac gccaagacca catatagatc caagaaaccc
     1321 gctaagaacc tcaagatgcc tggcgtctac tatgtggact acagactgga aagaatcaag
     1381 gaggccaaca acgaaaccta cgtcgagcag cacgcggtgg cagtggccag atactgcgac
     1441 ctccctagca aactggggca caaacttaat tgaagctagc tggctggata cccatacgat
     1501 gttccagatt acgctctcga gaccggttgg atttatgttg tatagattat attgagacaa
     1561 aattatctat ttgtatatat acataacagg gtaaattatt cagttaagaa aaaataattt
     1621 tatgaactgc atgtataaat gaagtttata cagtacagtg gttctacaat ctatttattg
     1681 gacatatcca tgacctgaaa ggaaacagtc atttgcgcac aactttaaaa gtctgcatta
     1741 cattcctcga taatgttgtg gtttgttgcc gttgccaaga attgaaaaca aaaagtttaa
     1801 aaaaaataat aataaattgc atgctgcttt aattgtgaat tgataataaa ctgtccctct
     1861 ttcagaaaac agattaaaaa aacaaaaaac aaaaaaaaaa acaaaaaaca aaaacaaaaa
     1921 ttggaaccaa aacattccgt ttacatttta gacactaagt atcttcgttc ttgttagtac
     1981 tctgttttac tgctttcgac ttctgatagc gttggaatta aaacaatgtc aaggtgctgt
     2041 tgtcattgct ttactggcgt aagggacggg gaatgggagg ggtagatttc tgtttgtttt
     2101 gtgttttatt ttgtttgttt gtttgttttg ttttttagtt ccacccggag tagggatgga
     2161 gaaaatttct tcactatcca ttctggttga taaagcgtta catttgtatg ttgtaaagat
     2221 gtttgcaaaa tccaatcaga tgactggaaa acaaataaaa attaaggcaa ctgaataaaa
     2281 tgctcacact ccactgccca tgatgtatct ccctggtccc cctcagctca ctcttctggc
     2341 atgggtcagg gaaaattgct tttattggaa agaccagcat ttgttcaaag catactcttt
     2401 ccctccctcc tcccattttg gtcccttctt tttgttttgt tttaagaaag aaaattaagt
     2461 tgcgcgcttt aaaatatttt actactgcta caaacagatg aacaatgtgt gtcattttat
     2521 gacactcatg gaaaacagtg attttttttt accctaaaga aaaacaaata aaaataaccc
     2581 aaaatattct ttttttaaaa ggcataaata ttgggtaaat tgtaatatgg cctaacagtg
     2641 tttgcagata aaagttattg tatacaccca gatacttaga taagagcagg gatccacact
     2701 gccattgaaa taggactgaa tggccctgcg gaggctaagt ggagctgaca tactatttcc
     2761 tggcagtgca ggaggaattt ctgagtggcc atcctaaggt ctaggatgga ggtggggaat
     2821 ggtacttgag acattcctaa aggaaggctc ggaagcaccc ttcagagcag gctctggaat
     2881 gatgtgtcaa gttgcttagg ccttctgctt taagtgccta cattacctaa cagtgctcaa
     2941 gaggttctcg attggagaac cacactcaaa tccatttata gcctccatcc catttctaaa
     3001 taattgtgta taaagttgga ttaacctgga gcaactttgg atccaaatat ggcacagcaa
     3061 taatgatatt aatgcagcat gatgggaaat gtttgctgtg aagagaattg atttgctttg
     3121 agcttagact tcaggaagcc taggtttttt atttttttat ttttgagaca ttttggtaaa
     3181 aggaaaaaaa gaaaacaaac aaacaaacaa acaaaaccag aaaaagcatc aaaactcagg
     3241 cagaatgagc aatgtctgaa agggctagaa aaacaagaca tagcaaggtg ctttcactgt
     3301 gaaagagaca agaacacagg aggaaatatt gcttcagtga agagcacaga cggctcctgc
     3361 caatttatta caagagtccc gtctgtactt taccctttgg ggttagaagt caagttggaa
     3421 gcctgaatga atggacccaa tgagaactag tgttaagccc atttccctag tcaggttttt
     3481 ttcaagcgtg aatgtgttag tggttactct cctgggttcc tgagcatcag aaaaaaaaaa
     3541 aaagaggcaa acaatcgctt catcttagga gtggaaagga aacagaagtg gacgtccgct
     3601 gtgactcagg gagtgaagat accatcagca aatagtttct tttttgttca ttcgttcctt
     3661 tcgagttagc ctgtcttttg gaataccact gaatatgctg tttttgaaag acttcatgta
     3721 gcatagattg ttttgtgccg tttaccaaat taacctttgt catcgttttt taacctattc
     3781 aggaatgctt ggaatatctg ctctatgtta actttttgca gcttcattct gagagacatt
     3841 agtcaaacaa acaaaaggat ccccatcaca atcttacagt actgcaaggg ccaggtctgt
     3901 taagcggctt cacaggagac atcagcaatt gtgtggtcag tggctggctc tcttacccac
     3961 taagatacat catagctaca tgttggtggt ttatgttgac ctgagattta tttgttaaaa
     4021 tctcttcttc gtttctgttc gttctggttc tgttctgttc tgttctgttc tgttttggtt
     4081 ttaaagtctt gctgtggtct cttgttggca gaaatgtttt atgcatggca gcaggcctgt
     4141 tgctttttta tagtgattcc cattgaaact gtaagtaaat gtctgtggcc ttgttctctc
     4201 tatggtaaag atattattca ccatgtaaaa caagaaaaaa tatttattgt attttagtat
     4261 atttatataa ttatgttatt gaaaaaattg gcattaaaac ttaaccacat cagaagccta
     4321 ttgtaaatac aggttctatt taagtgtacc aattaacata taatatatgt tttaaatagg
     4381 atccgggccc gaacaaaaac tcatctcaga agaggatctg aatagcgccg tcgaccatca
     4441 tcataatcat cattgagttt aaacccgctg atcagcctcg actgtgcctt ctagttgcca
     4501 gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac
     4561 tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat
     4621 tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca
     4681 tgctggggat gcggtgggct ctatggagat ctatcgatgt tctggggatg cggtgggctc
     4741 tatggcttct gaggcggaaa gaaccagctg gggctctagg gggtatcccc acgcgccctg
     4801 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
     4861 cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
     4921 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg
     4981 gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtacctaga agttcctatt
     5041 ccgaagttcc tattctctag aaagtatagg aacttccttg gccaaaaagc ctgaactcac
     5101 cgcgacgtct gtcgagaagt ttctgatcga aaagttcgac agcgtctccg acctgatgca
     5161 gctctcggag ggcgaagaat ctcgtgcttt cagcttcgat gtaggagggc gtggatatgt
     5221 cctgcgggta aatagctgcg ccgatggttt ctacaaagat cgttatgttt atcggcactt
     5281 tgcatcggcc gcgctcccga ttccggaagt gcttgacatt ggggaattca gcgagagcct
     5341 gacctattgc atctcccgcc gtgcacaggg tgtcacgttg caagacctgc ctgaaaccga
     5401 actgcccgct gttctgcagc cggtcgcgga ggccatggat gcgatcgctg cggccgatct
     5461 tagccagacg agcgggttcg gcccattcgg accgcaagga atcggtcaat acactacatg
     5521 gcgtgatttc atatgcgcga ttgctgatcc ccatgtgtat cactggcaaa ctgtgatgga
     5581 cgacaccgtc agtgcgtccg tcgcgcaggc tctcgatgag ctgatgcttt gggccgagga
     5641 ctgccccgaa gtccggcacc tcgtgcacgc ggatttcggc tccaacaatg tcctgacgga
     5701 caatggccgc ataacagcgg tcattgactg gagcgaggcg atgttcgggg attcccaata
     5761 cgaggtcgcc aacatcttct tctggaggcc gtggttggct tgtatggagc agcagacgcg
     5821 ctacttcgag cggaggcatc cggagcttgc aggatcgccg cggctccggg cgtatatgct
     5881 ccgcattggt cttgaccaac tctatcagag cttggttgac ggcaatttcg atgatgcagc
     5941 ttgggcgcag ggtcgatgcg acgcaatcgt ccgatccgga gccgggactg tcgggcgtac
     6001 acaaatcgcc cgcagaagcg cggccgtctg gaccgatggc tgtgtagaag tactcgccga
     6061 tagtggaaac cgacgcccca gcactcgtcc gagggcaaag gaatagcacg tactacgaga
     6121 tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc
     6181 cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc accccaactt
     6241 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
     6301 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
     6361 tgtctgtata ccgtcgacct ctagctagag cttggcgtaa tcatggtcat agctgtttcc
     6421 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
     6481 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
     6541 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
     6601 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
     6661 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     6721 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     6781 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     6841 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     6901 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     6961 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     7021 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     7081 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     7141 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     7201 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
     7261 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     7321 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     7381 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     7441 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     7501 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     7561 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     7621 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     7681 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
     7741 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     7801 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     7861 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
     7921 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
     7981 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
     8041 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
     8101 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
     8161 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
     8221 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
     8281 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
     8341 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
     8401 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
     8461 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
     8521 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtcgacggat cgggagatct
     8581 cccgatcccc tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag
     8641 tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt gcgcgagcaa aatttaagct
     8701 acaacaaggc aaggcttgac cgacaattgc atgaagaatc tgcttagggt taggcgtttt
     8761 gcgctgcttc gtacgggcca gatatacgc
//
