LOCUS       sequence-375615-       10825 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  An orthogonal trigger sequence used in plant experiments, derived 
            from the 3' UTR of murine Bdnf.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10825)
  AUTHORS   Kaseniit KE, Katz N, Kolber NS, Call CC, Wengier DL, Cody WB, 
            Sattely ES, Gao XJ
  TITLE     Modular, programmable RNA sensing using ADAR editing in living 
            cells.
  JOURNAL   Nat Biotechnol. 2022 Oct 5. pii: 10.1038/s41587-022-01493-x. doi: 
            10.1038/s41587-022-01493-x.
  PUBMED    36198772
REFERENCE   2  (bases 1 to 10825)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2022 Oct 5. pii: 10.1038/s41587-022-01493-x. doi: 
            10.1038/s41587-022-01493-x."
COMMENT     Sequence Label: CC0101 BFPmut-stop-Bdnf.t1 (pEAQ)
FEATURES             Location/Qualifiers
     source          1..10825
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        270..315
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     CDS             833..1543
                     /codon_start=1
                     /product="enhanced monomeric blue fluorescent protein 
                     (Subach et al., 2011)"
                     /label=mTagBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRND
                     MALKLVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHAVAV
                     ARYCDLPSKLGHKLN"
     CDS             1562..1588
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     terminator      1952..2204
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     promoter        2336..2681
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     polyA_signal    3308..3484
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        4050..4229
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        4082..4265
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             4286..5080
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4340..4359)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4950..4969
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     terminator      5466..5718
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    5809..5833
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     rep_origin      6201..6832
                     /label=oriV
                     /note="incP origin of replication"
     CDS             complement(6922..8070)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(8369..9163)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     primer_bind     complement(9753..9770)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(10004..10023)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    10455..10479
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     10618..10637
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10762..10784
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     10776..10793
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     10777..10793
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 ggaaacctcc tcggattcca ttgcccagct atctgtcact ttattgagaa gatagtggaa
       61 aaggaaggtg gctcctacaa atgccatcat tgcgataaag gaaaggccat cgttgaagat
      121 gcctctgccg acagtggtcc caaagatgga cccccaccca cgaggagcat cgtggaaaaa
      181 gaagacgttc caaccacgtc ttcaaagcaa gtggattgat gtgatatctc cactgacgta
      241 agggatgacg cacaatccca ctatccttcg caagaccctt cctctatata aggaagttca
      301 tttcatttgg agaggtatta aaatcttaat aggttttgat aaaagcgaac gtggggaaac
      361 ccgaaccaaa ccttcttcta aactctctct catctctctt aaagcaaact tctctcttgt
      421 ctttcttgcg tgagcgatct tcaacgttgt cagatcgtgc ttcggcacca gtacaacgtt
      481 ttctttcact gaagcgaaat caaagatctc tttgtggaca cgtagtgcgg cgccattaaa
      541 taacgtgtac ttgtcctatt cttgtcggtg tggtcttggg aaaagaaagc ttgctggagg
      601 ctgctgttca gccccataca ttacttgtta cgattctgct gactttcggc gggtgcaata
      661 tctctacttc tgcttgacga ggtattgttg cctgtacttc tttcttcttc ttcttgctga
      721 ttggttctat aagaaatcta gtattttctt tgaaacagag ttttcccgtg gttttcgaac
      781 ttggagaaag attgttaagc ttctgtatat tctgcccaaa ttcgcgaccg gtatggtgtc
      841 taagggcgaa gagctgatta aggagaacat gcacatgaag ctgtacatgg agggcaccgt
      901 ggacaaccat cacttcaagt gcacatccga gggcgaaggc aagccctacg agggcaccca
      961 gaccatgaga atcaaggtgg tcgagggcgg ccctctcccc ttcgccttcg acatcctggc
     1021 tactagcttc ctctacggca gcaagacctt catcaaccac acccagggca tccccgactt
     1081 cttcaagcag tccttccctg agggcttcac atgggagaga gtcaccacat acgaggacgg
     1141 gggcgtgctg accgctaccc aggacaccag cctccaggac ggctgcctca tctacaacgt
     1201 caagatcaga ggggtgaact tcacatccaa cggccctgtg atgcagaaga aaacactcgg
     1261 ctgggaggcc ttcaccgaaa cgctgtaccc cgctgacggc ggcctggaag gcagaaacga
     1321 catggccctg aagctcgtgg gcgggagcca tctgatcgca aacgccaaga ccacatatag
     1381 atccaagaaa cccgctaaga acctcaagat gcctggcgtc tactatgtgg actacagact
     1441 ggaaagaatc aaggaggcca acaacgaaac ctacgtcgag cagcacgcgg tggcagtggc
     1501 cagatactgc gacctcccta gcaaactggg gcacaaactt aattgaagct agctggctgg
     1561 atacccatac gatgttccag attacgctct cgagaccggt tagttccacc cggagtaggg
     1621 atggagaaaa tttcttcact atccattctg gttgataaag cgttacattt gtatgttgta
     1681 aagatgtttg ggatccgggc ccgtcgaggc ctttaactct ggtttcatta aattttcttt
     1741 agtttgaatt tactgttatt cggtgtgcat ttctatgttt ggtgagcggt tttctgtgct
     1801 cagagtgtgt ttattttatg taatttaatt tctttgtgag ctcctgttta gcaggtcgtc
     1861 ccttcagcaa ggacacaaaa agattttaat tttattaaaa aaaaaaaaaa aaaaaagacc
     1921 gggaattcga tatcaagctt atcgacctgc agatcgttca aacatttggc aataaagttt
     1981 cttaagattg aatcctgttg ccggtcttgc gatgattatc atataatttc tgttgaatta
     2041 cgttaagcat gtaataatta acatgtaatg catgacgtta tttatgagat gggtttttat
     2101 gattagagtc ccgcaattat acatttaata cgcgatagaa aacaaaatat agcgcgcaaa
     2161 ctaggataaa ttatcgcgcg cggtgtcatc tatgttacta gatctctaga gtctcaagct
     2221 tggcgcgcca gcttggcgta atcatggtca tagctgttgc gattaagaat tcgagctcgg
     2281 taccccccta ctccaaaaat gtcaaagata cagtctcaga agaccaaagg gctattgaga
     2341 cttttcaaca aagggtaatt tcgggaaacc tcctcggatt ccattgccca gctatctgtc
     2401 acttcatcga aaggacagta gaaaaggaag gtggctccta caaatgccat cattgcgata
     2461 aaggaaaggc tatcattcaa gatgcctctg ccgacagtgg tcccaaagat ggacccccac
     2521 ccacgaggag catcgtggaa aaagaagacg ttccaaccac gtcttcaaag caagtggatt
     2581 gatgtgacat ctccactgac gtaagggatg acgcacaatc ccactatcct tcgcaagacc
     2641 cttcctctat ataaggaagt tcatttcatt tggagaggac agcccaagct tcgactctag
     2701 aggatcccct taaatcgata tggaacgagc tatacaagga aacgacgcta gggaacaagc
     2761 taacagtgaa cgttgggatg gaggatcagg aggtaccact tctcccttca aacttcctga
     2821 cgaaagtccg agttggactg agtggcggct acataacgat gagacgaatt cgaatcaaga
     2881 taatcccctt ggtttcaagg aaagctgggg tttcgggaaa gttgtattta agagatatct
     2941 cagatacgac aggacggaag cttcactgca cagagtcctt ggatcttgga cgggagattc
     3001 ggttaactat gcagcatctc gatttttcgg tttcgaccag atcggatgta cctatagtat
     3061 tcggtttcga ggagttagta tcaccgtttc tggagggtct cgaactcttc agcatctctg
     3121 tgagatggca attcggtcta agcaagaact gctacagctt gccccaatcg aagtggaaag
     3181 taatgtatca agaggatgcc ctgaaggtac tcaaaccttc gaaaaagaaa gcgagtaagt
     3241 cgagggcgag ctcgaattcg gtacgctgaa atcaccagtc tctctctaca aatctatctc
     3301 tctctatttt ctccataaat aatgtgtgag tagtttcccg ataagggaaa ttagggttct
     3361 tatagggttt cgctcatgtg ttgagcatat aagaaaccct tagtatgtat ttgtatttgt
     3421 aaaatacttc tatcaataaa atttctaatt cctaaaacca aaatccagta ctaaaatcca
     3481 gatctcctaa agtccctata gatctttgtc gtgaatataa accagacacg agacgactaa
     3541 acctggagcc cagacgccgt tcgaagctag aagtaccgct taggcaggag gccgttaggg
     3601 aaaagatgct aaggcagggt tggttacgtt gactcccccg taggtttggt ttaaatatga
     3661 tgaagtggac ggaaggaagg aggaagacaa ggaaggataa ggttgcaggc cctgtgcaag
     3721 gtaagaagat ggaaatttga tagaggtacg ctactatact tatactatac gctaagggaa
     3781 tgcttgtatt tataccctat accccctaat aaccccttat caatttaaga aataatccgc
     3841 ataagccccc gcttaaaaat tggtatcaga gccatgaata ggtctatgac caaaactcaa
     3901 gaggataaaa cctcaccaaa atacgaaaga gttcttaact ctaaagataa aagatggcgc
     3961 gtggccggcc atgagcggag aattaaggga gtcacgttat gacccccgcc gatgacgcgg
     4021 gacaagccgt tttacgtttg gaactgacag aaccgcaacg ttgaaggagc cactcagccg
     4081 cgggtttctg gagtttaatg agctaagcac atacgtcaga aaccattatt gcgcgttcaa
     4141 aagtcgccta aggtcactat cagctagcaa atatttcttg tcaaaaatgc tccactgacg
     4201 ttccataaat tcccctcggt atccaattag agtctcatat tcactctcaa tccaaataat
     4261 ctgcaccgga tctggatcgt ttcgcatgat tgaacaagat ggattgcacg caggttctcc
     4321 ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc
     4381 tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga
     4441 cctgtccggt gccctgaatg aactgcagga cgaggcagcg cggctatcgt ggctggccac
     4501 gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct
     4561 gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa
     4621 agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc
     4681 attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct
     4741 tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc
     4801 caggctcaag gcgcgcatgc ccgacggcga tgatctcgtc gtgacccatg gcgatgcctg
     4861 cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct
     4921 gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct
     4981 tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca
     5041 gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga gcgggactct ggggttcgaa
     5101 atgaccgacc aagcgacgcc caacctgcca tcacgagatt tcgattccac cgccgccttc
     5161 tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc
     5221 ggggatctca tgctggagtt cttcgcccac gggatctctg cggaacaggc ggtcgaaggt
     5281 gccgatatca ttacgacagc aacggccgac aagcacaacg ccacgatcct gagcgacaat
     5341 atgatcgcgg cgtccacatc aacggcgtcg gcggcgactg cccaggcaag accgagatgc
     5401 accgcgatat cttgctgcgt tcggatattt tcgtggagtt cccgccacag acccggatga
     5461 tccccgatcg ttcaaacatt tggcaataaa gtttcttaag attgaatcct gttgccggtc
     5521 ttgcgatgat tatcatataa tttctgttga attacgttaa gcatgtaata attaacatgt
     5581 aatgcatgac gttatttatg agatgggttt ttatgattag agtcccgcaa ttatacattt
     5641 aatacgcgat agaaaacaaa atatagcgcg caaactagga taaattatcg cgcgcggtgt
     5701 catctatgtt actagatcgg gggccggccc tcactggtga aaagaaaaac caccccagta
     5761 cattaaaaac gtccgcaatg tgttattaag ttgtctaagc gtcaatttgt ttacaccaca
     5821 atatatcctg ccaccagcca gccaacagct ccccgaccgg cagctcggca caaaatcacc
     5881 actcgataca ggcagcccat cagtccggga cggcgtcagc gggagagccg ttgtaaggcg
     5941 gcagactttg ctcatgttac cgatgctatt cggaagaacg gcaactaagc tgccgggttt
     6001 gaaacacgga tgatctcgcg gagggtagca tgttgattgt aacgatgaca gagcgttgct
     6061 gcctgtgatc aaatatcatc tccctcgcag agatccgaat tatcagcctt cttattcatt
     6121 tctcgcttaa ccgtgacaga gtagacaggc tgtctcgcgg ccgaggggcg cagcccctgg
     6181 ggggatggga ggcccgcgtt agcgggccgg gagggttcga gaaggggggg cacccccctt
     6241 cggcgtgcgc ggtcacgcgc acagggcgca gccctggtta aaaacaaggt ttataaatat
     6301 tggtttaaaa gcaggttaaa agacaggtta gcggtggccg aaaaacgggc ggaaaccctt
     6361 gcaaatgctg gattttctgc ctgtggacag cccctcaaat gtcaataggt gcgcccctca
     6421 tctgtcagca ctctgcccct caagtgtcaa ggatcgcgcc cctcatctgt cagtagtcgc
     6481 gcccctcaag tgtcaatacc gcagggcact tatccccagg cttgtccaca tcatctgtgg
     6541 gaaactcgcg taaaatcagg cgttttcgcc gatttgcgag gctggccagc tccacgtcgc
     6601 cggccgaaat cgagcctgcc cctcatctgt caacgccgcg ccgggtgagt cggcccctca
     6661 agtgtcaacg tccgcccctc atctgtcagt gagggccaag ttttccgcga ggtatccaca
     6721 acgccggcgg ccgcggtgtc tcgcacacgg cttcgacggc gtttctggcg cgtttgcagg
     6781 gccatagacg gccgccagcc cagcggcgag ggcaaccagc ccggtgagcg tcggaaaggc
     6841 gctcggtctt gccttgctcg tcggtgatgt acactagtcg ctggctgctg aacccccagc
     6901 cggaactgac cccacaaggc cctagcgttt gcaatgcacc aggtcatcat tgacccaggc
     6961 gtgttccacc aggccgctgc ctcgcaactc ttcgcaggct tcgccgacct gctcgcgcca
     7021 cttcttcacg cgggtggaat ccgatccgca catgaggcgg aaggtttcca gcttgagcgg
     7081 gtacggctcc cggtgcgagc tgaaatagtc gaacatccgt cgggccgtcg gcgacagctt
     7141 gcggtacttc tcccatatga atttcgtgta gtggtcgcca gcaaacagca cgacgatttc
     7201 ctcgtcgatc aggacctggc aacgggacgt tttcttgcca cggtccagga cgcggaagcg
     7261 gtgcagcagc gacaccgatt ccaggtgccc aacgcggtcg gacgtgaagc ccatcgccgt
     7321 cgcctgtagg cgcgacaggc attcctcggc cttcgtgtaa taccggccat tgatcgacca
     7381 gcccaggtcc tggcaaagct cgtagaacgt gaaggtgatc ggctcgccga taggggtgcg
     7441 cttcgcgtac tccaacacct gctgccacac cagttcgtca tcgtcggccc gcagctcgac
     7501 gccggtgtag gtgatcttca cgtccttgtt gacgtggaaa atgaccttgt tttgcagcgc
     7561 ctcgcgcggg attttcttgt tgcgcgtggt gaacagggca gagcgggccg tgtcgtttgg
     7621 catcgctcgc atcgtgtccg gccacggcgc aatatcgaac aaggaaagct gcatttcctt
     7681 gatctgctgc ttcgtgtgtt tcagcaacgc ggcctgcttg gcctcgctga cctgttttgc
     7741 caggtcctcg ccggcggttt ttcgcttctt ggtcgtcata gttcctcgcg tgtcgatggt
     7801 catcgacttc gccaaacctg ccgcctcctg ttcgagacga cgcgaacgct ccacggcggc
     7861 cgatggcgcg ggcagggcag ggggagccag ttgcacgctg tcgcgctcga tcttggccgt
     7921 agcttgctgg accatcgagc cgacggactg gaaggtttcg cggggcgcac gcatgacggt
     7981 gcggcttgcg atggtttcgg catcctcggc ggaaaacccc gcgtcgatca gttcttgcct
     8041 gtatgccttc cggtcaaacg tccgattcat tcaccctcct tgcgggattg ccccgactca
     8101 cgccggggca atgtgccctt attcctgatt tgacccgcct ggtgccttgg tgtccagata
     8161 atccacctta tcggcaatga agtcggtccc gtagaccgtc tggccgtcct tctcgtactt
     8221 ggtattccga atcttgccct gcacgaatac cagcgacccc ttgcccaaat acttgccgtg
     8281 ggcctcggcc tgagagccaa aacacttgat gcggaagaag tcggtgcgct cctgcttgtc
     8341 gccggcatcg ttgcgccaca tctaggtact aaaacaattc atccagtaaa atataatatt
     8401 ttattttctc ccaatcaggc ttgatcccca gtaagtcaaa aaatagctcg acatactgtt
     8461 cttccccgat atcctccctg atcgaccgga cgcagaaggc aatgtcatac cacttgtccg
     8521 ccctgccgct tctcccaaga tcaataaagc cacttacttt gccatctttc acaaagatgt
     8581 tgctgtctcc caggtcgccg tgggaaaaga caagttcctc ttcgggcttt tccgtcttta
     8641 aaaaatcata cagctcgcgc ggatctttaa atggagtgtc ttcttcccag ttttcgcaat
     8701 ccacatcggc cagatcgtta ttcagtaagt aatccaattc ggctaagcgg ctgtctaagc
     8761 tattcgtata gggacaatcc gatatgtcga tggagtgaaa gagcctgatg cactccgcat
     8821 acagctcgat aatcttttca gggctttgtt catcttcata ctcttccgag caaaggacgc
     8881 catcggcctc actcatgagc agattgctcc agccatcatg ccgttcaaag tgcaggacct
     8941 ttggaacagg cagctttcct tccagccata gcatcatgtc cttttcccgt tccacatcat
     9001 aggtggtccc tttataccgg ctgtccgtca tttttaaata taggttttca ttttctccca
     9061 ccagcttata taccttagca ggagacattc cttccgtatc ttttacgcag cggtattttt
     9121 cgatcagttt tttcaattcc ggtgatattc tcattttagc catttattat ttccttcctc
     9181 ttttctacag tatttaaaga taccccaaga agctaattat aacaagacga actccaattc
     9241 actgttcctt gcattctaaa accttaaata ccagaaaaca gctttttcaa agttgttttc
     9301 aaagttggcg tataacatag tatcgacgga gccgattttg aaaccacaat tatgggtgat
     9361 gctgccaact tactgattta gtgtatgatg gtgtttttga ggtgctccag tggcttctgt
     9421 ttctatcagc tgtccctcct gttcagctac tgacggggtg gtgcgtaacg gcaaaagcac
     9481 cgccggacat cagcgctatc tctgctctca ctgccgtaaa acatggcaac tgcagttcac
     9541 ttacaccgct tctcaacccg gtacgcacca gaaaatcatt gatatggcca tgaatggcgt
     9601 tggatgccgg gcaacagccc gcattatggg cgttggcctc aacacgattt tacgtcactt
     9661 aaaaaactca ggccgcagtc ggtaactatg cggtgtgaaa taccgcacag atgcgtaagg
     9721 agaaaatacc gcatcaggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     9781 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     9841 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     9901 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     9961 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
    10021 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
    10081 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
    10141 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
    10201 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
    10261 tcgccactgg cagcaggtaa cctcgcgcat acagccgggc agtgacgtca tcgtctgcgc
    10321 ggaaatggac gggcccccgg cgccagatct ggggaaccct gtggttggca tgcacataca
    10381 aatggacgaa cggataaacc ttttcacgcc cttttaaata tccgattatt ctaataaacg
    10441 ctcttttctc ttaggtttac ccgccaatat atcctgtcaa acactgatag tttgtgaacc
    10501 atcacccaaa tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa
    10561 agggagcccc cgatttagag cttgacgggg aaagccggcg aacgtggcga gaaaggaagg
    10621 gaagaaagcg aaaggagcgg gcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg
    10681 tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa
    10741 gttgggtaac gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtgaattgt
    10801 taattaagaa ttcgagctcc accgc
//
