LOCUS       sequence-37569-e         976 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 976)
  AUTHORS   Petersen LK, Stowers RS
  TITLE     A Gateway MultiSite Recombination Cloning Toolkit.
  JOURNAL   PLoS One. 2011;6(9):e24531. Epub 2011 Sep 9.
  PUBMED    21931740
REFERENCE   2  (bases 1 to 976)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2011"; volume: "6(9)"; pages: "e24531. Epub 2011 Sep 9"
COMMENT     Sequence Label: pENTR L1-5XQUAS-R5
FEATURES             Location/Qualifiers
     source          1..976
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    68..92
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        278..516
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     463..484
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     protein_bind    552..675
                     /gene="mutant version of attR"
                     /label=attR5
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(730..749)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(731..749)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(754..770)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(754..770)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(872..891)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(950..969)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 gattttattt tgactgatag tgacctgttc gttgcaacaa attgatgagc aatgcttttt
       61 tataatgcca actttgtaca aaaaagcagg ctctatccaa cgcgttggga gctctccgga
      121 tccaagcttg gatccgggta atcgcttatc ctcggataaa caattatcct cacgggtaat
      181 cgcttatccg ctcgggtaat cgcttatcct cgggtaatcg cttatcctta agcttgatat
      241 cgaattgctg cagcccgagc ggagactcta gcgagcgccg gagtataaat agaggcgctt
      301 cgtctacgga gcgacaattc aattcaaaca agcaaagtga acacgtcgct aagcgaaagc
      361 taagcaaata aacaagcgca gctgaacaag ctaaacaatc tgcagtaaag tgcaagttaa
      421 agtgaatcaa ttaaaagtaa ccagcaacca agtaaatcaa ctgcaactac tgaaatctgc
      481 caagaagtaa ttattgaata caagaagaga actctgaata gggaattggg aattcgttaa
      541 cagatcgggg acaactttgt atacaaaagt tgaacgagaa acgtaaaatg atataaatat
      601 caatatatta aattagattt tgcataaaaa acagactaca taatactgta aaacacaaca
      661 tatccagtca ctatgaatca actacttaga tggtattagt gacctgtact gcagggcggc
      721 cgcgatatcc cctatagtga gtcgtattac atggtcatag ctgtttcctg gcagctctgg
      781 cccgtgtctc aaaatctctg atgttacatt gcacaagata aaaatatatc atcatgaaca
      841 ataaaactgt ctgcttacat aaacagtaat acaaggggtg ttatgagcca tattcaacgg
      901 gaaacgtcga ggccgcgatt aaattccaac atggatgctg atttatatgg gtataaatgg
      961 gctcgcgata atgtcg
//
