LOCUS       sequence-37570-e         946 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 946)
  AUTHORS   Petersen LK, Stowers RS
  TITLE     A Gateway MultiSite Recombination Cloning Toolkit.
  JOURNAL   PLoS One. 2011;6(9):e24531. Epub 2011 Sep 9.
  PUBMED    21931740
REFERENCE   2  (bases 1 to 946)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2011"; volume: "6(9)"; pages: "e24531. Epub 2011 Sep 9"
COMMENT     Sequence Label: pENTR L5-GAL4-L2
FEATURES             Location/Qualifiers
     source          1..946
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    69..89
                     /gene="mutant version of attB"
                     /label=attB5
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     CDS             96..536
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
ORIGIN
        1 gattttattt tgactgatag tgacctgttc gttgcaacaa attgatgagc aatgcttttt
       61 tataatgcca actttgtata caaaagttgg ccaacatgaa gctactgtct tctatcgaac
      121 aagcatgcga tatttgccga cttaaaaagc tcaagtgctc caaagaaaaa ccgaagtgcg
      181 ccaagtgtct gaagaacaac tgggagtgtc gctactctcc caaaaccaaa aggtctccgc
      241 tgactagggc acatctgaca gaagtggaat caaggctaga aagactggaa cagctatttc
      301 tactgatttt tcctcgagaa gaccttgaca tgattttgaa aatggattct ttacaggata
      361 taaaagcatt gttaacagga ttatttgtac aagataatgt gaataaagat gccgtcacag
      421 atagattggc ttcagtggag actgatatgc ctctaacatt gagacagcat agaataagtg
      481 cgacatcatc atcggaagag agtagtaaca aaggtcaaag acagttgact gtatcgattg
      541 actcggcagc tcatcatgat aactccacaa ttccgttgga ttttatgccc agggatgctc
      601 ttcatggatt tgattggtct gaagaggatg acatgtcgga tggcttgccc ttcctgaaaa
      661 cggaccccaa caataatggg ttctttggcg acggttctct cttatgtatt cttcgatcta
      721 ttggctttaa accggaaaat tacacgaact ctaacgttaa caggctcccg accatgatta
      781 cggatagata cacgttggct tctagatcca caacatcccg tttacttcaa agttatctca
      841 ataattttca cccctactgc cctatcgtgc actcaccgac gctaatgatg ttgtataata
      901 accagattga aatcgcgtcg aaggatcaat ggcaaatcct ttttaa
//
