LOCUS       sequence-37572-e         951 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 951)
  AUTHORS   Petersen LK, Stowers RS
  TITLE     A Gateway MultiSite Recombination Cloning Toolkit.
  JOURNAL   PLoS One. 2011;6(9):e24531. Epub 2011 Sep 9.
  PUBMED    21931740
REFERENCE   2  (bases 1 to 951)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2011"; volume: "6(9)"; pages: "e24531. Epub 2011 Sep 9"
COMMENT     Sequence Label: pENTR L1-5XQUAS-L4
FEATURES             Location/Qualifiers
     source          1..951
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    73..97
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        283..521
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     468..489
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     protein_bind    complement(560..655)
                     /gene="mutant version of attL"
                     /label=attL4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(686..705)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(687..705)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(710..726)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(710..726)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(828..847)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(906..925)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 atatggattt tattttgact gatagtgacc tgttcgttgc aacaaattga tgagcaatgc
       61 ttttttataa tgccaacttt gtacaaaaaa gcaggctcta tccaacgcgt tgggagctct
      121 ccggatccaa gcttggatcc gggtaatcgc ttatcctcgg ataaacaatt atcctcacgg
      181 gtaatcgctt atccgctcgg gtaatcgctt atcctcgggt aatcgcttat ccttaagctt
      241 gatatcgaat tgctgcagcc cgagcggaga ctctagcgag cgccggagta taaatagagg
      301 cgcttcgtct acggagcgac aattcaattc aaacaagcaa agtgaacacg tcgctaagcg
      361 aaagctaagc aaataaacaa gcgcagctga acaagctaaa caatctgcag taaagtgcaa
      421 gttaaagtga atcaattaaa agtaaccagc aaccaagtaa atcaactgca actactgaaa
      481 tctgccaaga agtaattatt gaatacaaga agagaactct gaatagggaa ttgggaattc
      541 gttaacagat cggggcaccc aacttttcta tacaaagttg gcattataag aaagcattgc
      601 ttatcaattt gttgcaacga acaggtcact atcagtcaaa ataaaatcat tatttgccat
      661 ccagctgcag ggcggccgcg atatccccta tagtgagtcg tattacatgg tcatagctgt
      721 ttcctggcag ctctggcccg tgtctcaaaa tctctgatgt tacattgcac aagataaaaa
      781 tatatcatca tgaacaataa aactgtctgc ttacataaac agtaatacaa ggggtgttat
      841 gagccatatt caacgggaaa cgtcgaggcc gcgattaaat tccaacatgg atgctgattt
      901 atatgggtat aaatgggctc gcgataatgt cgggcaatca ggtgcgacaa t
//
