LOCUS       sequence-37573-e         903 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 903)
  AUTHORS   Petersen LK, Stowers RS
  TITLE     A Gateway MultiSite Recombination Cloning Toolkit.
  JOURNAL   PLoS One. 2011;6(9):e24531. Epub 2011 Sep 9.
  PUBMED    21931740
REFERENCE   2  (bases 1 to 903)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2011"; volume: "6(9)"; pages: "e24531. Epub 2011 Sep 9"
COMMENT     Sequence Label: pENTR R4-GAL4-R3
FEATURES             Location/Qualifiers
     source          1..903
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(24..148)
                     /gene="mutant version of attR"
                     /label=attR4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             154..594
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
ORIGIN
        1 taataccatc taagtagttg attcatagtg actggatatg ttgtgtttta cagtattatg
       61 tagtctgttt tttatgcaaa atctaattta atatattgat atttatatca ttttacgttt
      121 ctcgttcaac ttttctatac aaagttggcc aacatgaagc tactgtcttc tatcgaacaa
      181 gcatgcgata tttgccgact taaaaagctc aagtgctcca aagaaaaacc gaagtgcgcc
      241 aagtgtctga agaacaactg ggagtgtcgc tactctccca aaaccaaaag gtctccgctg
      301 actagggcac atctgacaga agtggaatca aggctagaaa gactggaaca gctatttcta
      361 ctgatttttc ctcgagaaga ccttgacatg attttgaaaa tggattcttt acaggatata
      421 aaagcattgt taacaggatt atttgtacaa gataatgtga ataaagatgc cgtcacagat
      481 agattggctt cagtggagac tgatatgcct ctaacattga gacagcatag aataagtgcg
      541 acatcatcat cggaagagag tagtaacaaa ggtcaaagac agttgactgt atcgattgac
      601 tcggcagctc atcatgataa ctccacaatt ccgttggatt ttatgcccag ggatgctctt
      661 catggatttg attggtctga agaggatgac atgtcggatg gcttgccctt cctgaaaacg
      721 gaccccaaca ataatgggtt ctttggcgac ggttctctct tatgtattct tcgatctatt
      781 ggctttaaac cggaaaatta cacgaactct aacgttaaca ggctcccgac catgattacg
      841 gatagataca cgttggcttc tagatccaca acatcccgtt tacttcaaag ttatctcaat
      901 aat
//
