LOCUS       sequence-375732-       11021 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for tri-cistronic expression of EGFP, MDH1 and ME1
            (seperated by P2A and T2A).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11021)
  AUTHORS   Igelmann S, Lessard F, Uchenunu O, Bouchard J, Fernandez-Ruiz A, 
            Rowell MC, Lopes-Paciencia S, Papadopoli D, Fouillen A, Ponce KJ, 
            Huot G, Mignacca L, Benfdil M, Kalegari P, Wahba HM, Pencik J, Vuong
            N, Quenneville J, Guillon J, Bourdeau V, Hulea L, Gagnon E, Kenner 
            L, Moriggl R, Nanci A, Pollak MN, Omichinski JG, Topisirovic I, 
            Ferbeyre G
  TITLE     A hydride transfer complex reprograms NAD metabolism and bypasses 
            senescence.
  JOURNAL   Mol Cell. 2021 Sep 16;81(18):3848-3865.e19. doi: 
            10.1016/j.molcel.2021.08.028.
  PUBMED    34547241
REFERENCE   2  (bases 1 to 11021)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2021.08"; journalName: "Mol Cell"; date: 
            "2021-09-16- 16"; volume: "81"; issue: "18"; pages: "3848-3865.e19"
COMMENT     Sequence Label: pUltra-MDH1-ME1
FEATURES             Location/Qualifiers
     source          1..11021
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     94..111
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    228..249
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(264..269,270..287,288..294)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    302..318
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     307..329
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     326..342
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     326..342
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     361..381
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        363..381
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        409..635
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             636..816
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    863..988
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1481..1714
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1899..1943
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2241..2358
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2423..3634
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     3471..3490
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     regulatory      3667..3676
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3673..3675,3676..3678,3679..4389)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3673..3675,3676..3678,3679..4389)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3718..3739)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3979..3998)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4326..4347
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             4399..4455
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             5479..5532
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     misc_feature    7269..7857
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7322..7342)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7740..7751)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7948..8181
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8253..8374
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8290..8309)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8344..8363
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8414..8549
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8569..8588)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8570..8588)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8598..8615)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8598..8614)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8607..8629)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8756..9211
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8843..8862)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9053..9074
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9237..9341
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9342..9410,9411..10202)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9560..9579)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10373..10961
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10862..10881
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
       61 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
      121 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg
      181 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag
      241 ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga
      301 attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta cgccaagcgc
      361 gcaattaacc ctcactaaag ggaacaaaag ctggagctgc aagcttaatg tagtcttatg
      421 caatactctt gtagtcttgc aacatggtaa cgatgagtta gcaacatgcc ttacaaggag
      481 agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg gtacgatcgt gccttattag
      541 gaaggcaaca gacgggtctg acatggattg gacgaaccac tgaattgccg cattgcagag
      601 atattgtatt taagtgccta gctcgataca taaacgggtc tctctggtta gaccagatct
      661 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
      721 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
      781 tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa
      841 agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg aagcgcgcac
      901 ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta gcggaggcta
      961 gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta gatcgcgatg
     1021 ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac atatagtatg
     1081 ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa catcagaagg
     1141 ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag aagaacttag
     1201 atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag agataaaaga
     1261 caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga ccaccgcaca
     1321 gcaagcggcc gctgatcttc agacctggag gaggagatat gagggacaat tggagaagtg
     1381 aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc accaaggcaa
     1441 agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg ttccttgggt
     1501 tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg gtacaggcca
     1561 gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct attgaggcgc
     1621 aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca agaatcctgg
     1681 ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc tctggaaaac
     1741 tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct ctggaacaga
     1801 tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac acaagcttaa
     1861 tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg
     1921 aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg ctgtggtata
     1981 taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt tttgctgtac
     2041 tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag acccacctcc
     2101 caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga gagagagaca
     2161 gagacagatc cattcgatta gtgaacggat cggcactgcg tgcgccaatt ctgcagacaa
     2221 atggcagtat tcatccacaa ttttaaaaga aaagggggga ttggggggta cagtgcaggg
     2281 gaaagaatag tagacataat agcaacagac atacaaacta aagaattaca aaaacaaatt
     2341 acaaaaattc aaaattttcg ggtttattac agggacagca gagatccagt ttggttaatt
     2401 aacccgtgtc ggctccagat ctggcctccg cgccgggttt tggcgcctcc cgcgggcgcc
     2461 cccctcctca cggcgagcgc tgccacgtca gacgaagggc gcagcgagcg tcctgatcct
     2521 tccgcccgga cgctcaggac agcggcccgc tgctcataag actcggcctt agaaccccag
     2581 tatcagcaga aggacatttt aggacgggac ttgggtgact ctagggcact ggttttcttt
     2641 ccagagagcg gaacaggcga ggaaaagtag tcccttctcg gcgattctgc ggagggatct
     2701 ccgtggggcg gtgaacgccg atgattatat aaggacgcgc cgggtgtggc acagctagtt
     2761 ccgtcgcagc cgggatttgg gtcgcggttc ttgtttgtgg atcgctgtga tcgtcacttg
     2821 gtgagtagcg ggctgctggg ctggccgggg ctttcgtggc cgccgggccg ctcggtggga
     2881 cggaagcgtg tggagagacc gccaagggct gtagtctggg tccgcgagca aggttgccct
     2941 gaactggggg ttggggggag cgcagcaaaa tggcggctgt tcccgagtct tgaatggaag
     3001 acgcttgtga ggcgggctgt gaggtcgttg aaacaaggtg gggggcatgg tgggcggcaa
     3061 gaacccaagg tcttgaggcc ttcgctaatg cgggaaagct cttattcggg tgagatgggc
     3121 tggggcacca tctggggacc ctgacgtgaa gtttgtcact gactggagaa ctcggtttgt
     3181 cgtctgttgc gggggcggca gttatggcgg tgccgttggg cagtgcaccc gtacctttgg
     3241 gagcgcgcgc cctcgtcgtg tcgtgacgtc acccgttctg ttggcttata atgcagggtg
     3301 gggccacctg ccggtaggtg tgcggtaggc ttttctccgt cgcaggacgc agggttcggg
     3361 cctagggtag gctctcctga atcgacaggc gccggacctc tggtgagggg agggataagt
     3421 gaggcgtcag tttctttggt cggttttatg tacctatctt cttaagtagc tgaagctccg
     3481 gttttgaact atgcgctcgg ggttggcgag tgtgttttgt gaagtttttt aggcaccttt
     3541 tgaaatgtaa tcatttgggt caatatgtaa ttttcagtgt tagactagta aattgtccgc
     3601 taaattctgg ccgtttttgg cttttttgtt agacgaagct tgggctgcag gtccgatcca
     3661 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
     3721 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
     3781 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
     3841 ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc
     3901 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
     3961 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
     4021 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
     4081 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac
     4141 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc
     4201 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
     4261 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc
     4321 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
     4381 ctgtacaagg gatctggagc aacaaacttc tcactactca aacaagcagg tgacgtggag
     4441 gagaatcccg ggccttctag aatgtctgaa ccaatcagag tccttgtgac tggagcagct
     4501 ggtcaaattg catattcact gctgtacagt attggaaatg gatctgtctt tggtaaagat
     4561 cagcctataa ttcttgtgct gttggatatc acccccatga tgggtgtcct ggacggtgtc
     4621 ctaatggaac tgcaagactg tgcccttccc ctcctgaaag atgtcatcgc aacagataaa
     4681 gaagacgttg ccttcaaaga cctggatgtg gccattcttg tgggctccat gccaagaagg
     4741 gaaggcatgg agagaaaaga tttactgaaa gcaaatgtga aaatcttcaa atcccagggt
     4801 gcagccttag ataaatacgc caagaagtca gttaaggtta ttgttgtggg taatccagcc
     4861 aataccaact gcctgactgc ttccaagtca gctccatcca tccccaagga gaacttcagt
     4921 tgcttgactc gtttggatca caaccgagct aaagctcaaa ttgctcttaa acttggtgtg
     4981 actgctaatg atgtaaagaa tgtcattatc tggggaaacc attcctcgac tcagtatcca
     5041 gatgtcaacc atgccaaggt gaaattgcaa ggaaaggaag ttggtgttta tgaagctctg
     5101 aaagatgaca gctggctcaa gggagaattt gtcacgactg tgcagcagcg tggcgctgct
     5161 gtcatcaagg ctcgaaaact atccagtgcc atgtctgctg caaaagccat ctgtgaccac
     5221 gtcagggaca tctggtttgg aaccccagag ggagagtttg tgtccatggg tgttatctct
     5281 gatggcaact cctatggtgt tcctgatgat ctgctctact cattccctgt tgtaatcaag
     5341 aataagacct ggaagtttgt tgaaggtctc cctattaatg atttctcacg tgagaagatg
     5401 gatcttactg caaaggaact gacagaagaa aaagaaagtg cttttgaatt tctttcctct
     5461 gccggatccg gctccggcga gggcagggga agtctactaa catgcgggga cgtggaggaa
     5521 aatcccggcc cagctagcat ggagcccgaa gccccccgtc gccgccacac ccatcagcgc
     5581 ggctacctgc tgacacggaa ccctcacctc aacaaggact tggcctttac cctggaagag
     5641 agacagcaat tgaacattca tggattgttg ccaccttcct tcaacagtca ggagatccag
     5701 gttcttagag tagtaaaaaa tttcgagcat ctgaactctg actttgacag gtatcttctc
     5761 ttaatggatc tccaagatag aaatgaaaaa ctcttttata gagtgctgac atctgacatt
     5821 gagaaattca tgcctattgt ttatactccc actgtgggtc tggcttgcca acaatatagt
     5881 ttggtgtttc ggaagccaag aggtctcttt attactatcc acgatcgagg gcatattgct
     5941 tcagttctca atgcatggcc agaagatgtc atcaaggcca ttgtggtgac tgatggagag
     6001 cgtattcttg gcttgggaga ccttggctgt aatggaatgg gcatccctgt gggtaaattg
     6061 gctctatata cagcttgcgg agggatgaat cctcaagaat gtctgcctgt cattctggat
     6121 gtgggaaccg aaaatgagga gttacttaaa gatccactct acattggact acggcagaga
     6181 agagtaagag gttctgaata tgatgatttt ttggacgaat tcatggaggc agtttcttcc
     6241 aagtatggca tgaattgcct tattcagttt gaagattttg ccaatgtgaa tgcatttcgt
     6301 ctcctgaaca agtatcgaaa ccagtattgc acattcaatg atgatattca aggaacagca
     6361 tctgttgcag ttgcaggtct ccttgcagct cttcgaataa ccaagaacaa actgtctgat
     6421 caaacaatac tattccaagg agctggagag gctgccctag ggattgcaca cctgattgtg
     6481 atggccttgg aaaaagaagg tttaccaaaa gagaaagcca tcaaaaagat atggctggtt
     6541 gattcaaaag gattaatagt taagggacgt gcttccttaa cacaagagaa agagaagttt
     6601 gcccatgaac atgaagaaat gaagaaccta gaagccattg ttcaagaaat aaaaccaact
     6661 gccctcatag gagttgctgc aattggtggt gcattctcag aacaaattct caaagatatg
     6721 gctgccttca atgaacggcc tattattttt gctttgagta atccaactag caaagcagaa
     6781 tgttctgcag agcagtgcta caaaataacc aagggacgtg caatttttgc cagtggcagt
     6841 ccttttgatc cagtcactct tccaaatgga cagaccctat atcctggcca aggcaacaat
     6901 tcctatgtgt tccctggagt tgctcttggt gttgtggcgt gtggattgag gcagatcaca
     6961 gataatattt tcctcactac tgctgaggtt atagctcagc aagtgtcaga taaacacttg
     7021 gaagagggtc ggctttatcc tcctttgaat accattagag atgtttctct gaaaattgca
     7081 gaaaagattg tgaaagatgc ataccaagaa aagacagcca cagtttatcc tgaaccgcaa
     7141 aacaaagaag catttgtccg ctcccagatg tatagtactg attatgacca gattctacct
     7201 gattgttatt cttggcctga agaggtgcag aaaatacaga ccaaagttga ccagtaggtc
     7261 gactcgacaa tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact
     7321 atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg
     7381 cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg
     7441 aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa
     7501 cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc
     7561 ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg
     7621 ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaagctgacg tcctttccat
     7681 ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt
     7741 cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc
     7801 cgcatcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcctg
     7861 gaattaattc gagctcggta cctttaagac caatgactta caaggcagct gtagatctta
     7921 gccacttttt aaaagaaaag gggggactgg aagggctacg taactcccaa cgaagacaag
     7981 atctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct
     8041 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag
     8101 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt
     8161 cagtgtggaa aatctctagc agtagtagtt catgtcatct tattattcag tatttataac
     8221 ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt tattgcagct tataatggtt
     8281 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
     8341 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggctctag ctatcccgcc
     8401 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
     8461 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca
     8521 gaagtagtga ggaggctttt ttggaggcct agggacgtac ccaattcgcc ctatagtgag
     8581 tcgtattacg cgcgctcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc
     8641 gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa
     8701 gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atgggacgcg
     8761 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
     8821 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
     8881 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
     8941 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg
     9001 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
     9061 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
     9121 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
     9181 aattttaaca aaatattaac gcttacaatt taggtggcac ttttcgggga aatgtgcgcg
     9241 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
     9301 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
     9361 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
     9421 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
     9481 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
     9541 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag
     9601 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
     9661 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
     9721 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
     9781 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
     9841 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa
     9901 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
     9961 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
    10021 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
    10081 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
    10141 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
    10201 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
    10261 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
    10321 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
    10381 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
    10441 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
    10501 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact
    10561 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
    10621 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
    10681 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
    10741 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
    10801 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
    10861 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctca cttgagcgtc
    10921 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
    10981 ttttacggtt cctggccttt tgctggcctt ttgctcacat g
//
