LOCUS       sequence-375743-        7044 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  E. coli - Eggerthella lenta shuttle plasmid with tetracycline 
            resistance in E. lenta.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7044)
  AUTHORS   Dong X, Guthrie BGH, Alexander M, Noecker C, Ramirez L, Glasser NR, 
            Turnbaugh PJ, Balskus EP
  TITLE     Genetic manipulation of the human gut bacterium Eggerthella lenta 
            reveals a widespread family of transcriptional regulators.
  JOURNAL   Nat Commun. 2022 Dec 9;13(1):7624. doi: 10.1038/s41467-022-33576-3.
  PUBMED    36494336
REFERENCE   2  (bases 1 to 7044)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-12-9"; volume: "13(1):7624. doi"; pages: " 
            10.1038/s41467-022-33576-3"
COMMENT     Sequence Label: pXD69m1-TetW
FEATURES             Location/Qualifiers
     source          1..7044
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(4944..4962)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5030..5134
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5135..5203,5204..5995)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5353..5372)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6166..6754
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6655..6674
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(6935..7006)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 cattcgagac aaggagatta tagcatggcg gcaaacgctg gcgagacgaa ggtgaagctc
       61 tgggagggca tctgctaccc agagaacatg cgcgatgact ggcaggacga gatagacgac
      121 attcttcaag tgcctttcgc ttatgcggtt cacgacatag accatgacca gaagagcaag
      181 cagcgcaaga cgcatgcgca cgtcattgtt gtctggggcg gcaacacgac acgcaaggca
      241 atcatcaacg tcttgaaccg attgagcgcg gacggcaaga agtgctgttc gtcagccgag
      301 ccaatcaaca acatcaggca tgcgtatgac tatctgattc acgacacggc ttcctgtcgc
      361 aagaagggca aggagctgta tccggtcgaa gcacgcatcg agggaaacaa cttcgacatt
      421 ggtcagctcg aacagctctc gactaaggac aagcaggaca tgctcttcga gttggtcggc
      481 ttcatcatgt gcgagaggtt cgagaccatc aacgatttca cggctgcggc gctgagggag
      541 tttcccgagc agtaccgcga aatcatcgtg gggtacaact cgattctcga gcgctattgt
      601 cgcgggaact atctgaacgc cgaacgtaag cgcaagtgcg tggaacagtc aggtgccaaa
      661 gagtagtcgg gtcaatgcag caatgcgtga ctacggggcg gcgtggtctg cgctcgcgca
      721 gatctaagcc gactcgttca cggattgcgg aattgacgcg acggccaacc gaatagacca
      781 gacttgatta agggtcgatg gacgctctgc tccatcgacc ctttccttgc ccgtgtctcg
      841 actggatgag acctgcagaa tcgggcggct cattattttt cgctaactac gtccgaaaaa
      901 taatgtgtcc gaaaaataat gtgacaaatg acaaatgaca aatgaccaca ggggggccac
      961 tacgacggcc cccctaatag tgccgtgtgc cgtgtgccga accgcaggta gattgagggg
     1021 tggttcggag gggcgaagtc cctccgttcg gcgctcgcgc cgatggcccg agggccgtga
     1081 gcgaagccgc gaacgggcaa gggctggggg gtctcggggg gaacgcccga gcgggtgcag
     1141 ggctggccct gccacgtcct tagcgtagct aaggtctaac gtgctagact ttagtcgatt
     1201 aagtctgtac ggttcggagg tttccatggc tcacatcgcg aaatacaagg ctccatcggt
     1261 cgggcacatg cttgcccact accgccgcga ccgcagcagc ctggagcgcg acaacatcga
     1321 ccccgagcgc atcaagaacg acttggtggt cggccactac acgaacaagg acggcaagct
     1381 cgttgtgggg cgcgtggagc cgcgcgatgg cgagccgaac tggggcacgg tggagagccg
     1441 catcgagcgc gtgaacgagg cccagaaggc cgcagggaag cgtgccacgc gcaaggatgc
     1501 ggtcgttatg gctgacgttg ttgtgacgct tcccgacaat gtgcgcaagg gcgatgagga
     1561 caggttcttc cgtctcacgt actggtacct gtcgaagaag ttcggcatcg agaacatgat
     1621 gggcgggtac gtccacaagg acgaggtgtt gaaggatggc acgcccgccc gcgaccacat
     1681 gcacgtgccg ttcacgccca tcctagatgg acggttcaac tacaagcaga tgtgcccccg
     1741 cagcttctac cggagcatgc acaaggagct tggggattac ctagaggggc gcatgggcta
     1801 ccgccctgca atcgagcttg acgaggagac gcgggcgcag cgtgtataca cggacaagtc
     1861 cgtggatatc gacaaggtac gctgtgcggt cgataaggcc gtggttgaac ctgcgaagga
     1921 cgaagcggcg cgaatcgtcg cggcggctag ggaagaagcc gccgctttac tgaatgatgc
     1981 cgaaacgcgt aaggcagagc ttgttaccga gattgccgac aaggagggtg accttgccga
     2041 gcttgacagc cagctcgagg acgtgaagct cgatatcgaa gatgagcaag accgattgga
     2101 gtgtcttcgg caacgagcgg acggagttgc gcgagacatt ggagagcttc aacccatcgc
     2161 taccgaggtt cggggctggg aagctgcggg aaaagctgag cgcggcgcaa tcctcgataa
     2221 catcgttgtt aagtgcgatg gactcgcgag ccgcatcaga gcaggagttg cgggaatgcg
     2281 aatcaaagtg gaagagctta gaagtcgaat atcgcgcccg ctcgaatatc tgatgcgcag
     2341 ggaacagcca aggcaacaga gccttaatga cgtgctgcgc gatgcgcagc gggcggcgga
     2401 tgcgtggaac cgcgaccacg ctgcaccgca gaggacgtat cgcgggcggg ctagatgatg
     2461 tgttatagta gtacctgtaa tacacgacaa ggaggtgcga cgcgaaaaaa ccccgctgaa
     2521 gcggggtttt ttgcgcacca cagcagacag cagaaaacat tctgaacgct aggaagccgg
     2581 tatgattgtt acatataagg ggaagaaaaa tttcttttag gtacttgctt tcctaaaact
     2641 gatgtgatac aatgatttaa tccagaaaag gagtaaaaaa tatgcggcaa ggtattctta
     2701 aataaaacta taatcaaata gtgggaacaa aggattatga tagctccttt tgtagggact
     2761 tagttttttg tacccaattt aagaatactt ttgccttatc aattttgaca tatccccaaa
     2821 aacagcaatc acaaacaggt gtatgctgta tatgtgtatg tccgcaactt ataatcccca
     2881 gtggtaaaag tattttactg ctggggattt ttatgccctt tggggctgta aagggaggac
     2941 aatcacatga aaataatcaa tattggaatt cttgcccatg tagacgctgg aaagacgacc
     3001 ttgacggaga gcctgctata tgccagcgga gccatttcag aaccggggag cgtcgaaaaa
     3061 gggacaacga ggacggacac catgtttttg gagcggcagc gtgggattac cattcaagcg
     3121 gcagtcactt ccttccagtg gcacagatgt aaagttaaca ttgtggatac gcccggccac
     3181 atggattttt tggcggaggt gtaccgctct ttggctgttt tagatggggc catcttggtg
     3241 atctccgcta aagatggcgt gcaggcccag acccgtattc tgttccatgc cctgcggaaa
     3301 atgaacattc ccaccgttat ctttatcaac aagatcgacc aggctggcgt tgatttgcag
     3361 agcgtggttc agtctgttcg ggataagctc tccgccgata ttatcatcaa gcagacggtg
     3421 tcgctgtccc cggaaatagt cctggaggaa aataccgaca tagaagcatg ggatgcggtc
     3481 atcgaaaata acgatgaatt attggaaaag tatatcgcag gagaaccaat cagccgggaa
     3541 aaacttgcgc gggaggaaca gcagcgggtt caagacgcct ccctgttccc agtctatcat
     3601 ggcagcgcca aaaatggcct tggcattcaa ccgttgatgg atgcggtgac agggctgttc
     3661 caaccgattg gggaacaggg gggcgccgcc ctatgcggca gcgttttcaa ggttgagtac
     3721 accgattgcg gccagcggcg tgtctatcta cggttataca gcggaacgct gcgcctgcgg
     3781 gatacggtgg ccctggccgg gagagaaaag ctgaaaatca cagagatgcg tattccatcc
     3841 aaaggggaaa ttgttcggac agacaccgct tatcagggtg aaattgttat ccttcccagc
     3901 gacagcgtga ggttaaacga tgtattaggg gaccaaaccc ggctccctcg taaaaggtgg
     3961 cgcgaggacc ccctccccat gctgcggacg gcgattgcgc cgaaaacggc agcgcaaaga
     4021 gaacggctgc tggacgctct tacgcaactt gcggatactg acccgctttt gcgttgcgaa
     4081 gtggattcca tcacccatga gatcattctt tcttttttgg gccgggtgca gttggaggtt
     4141 gtttccgctt tgctgtcgga aaaatacaag cttgaaacag tggtaaagga accctccgtc
     4201 atttatatgg agcggccgct caaagcagcc agccacacca tccatatcga ggtgccgccc
     4261 aacccgtttt gggcatccat aggactgtct gttacaccac tctcgcttgg ctccggtgta
     4321 caatacgaga gccgggtttc gctgggatac ttgaaccaga gttttcaaaa cgctgtcagg
     4381 gatggtatcc gttacgggct ggagcagggc ttgttcggct ggaacgtaac ggactgtaag
     4441 atttgctttg aatacgggct ttattacagt ccggtcagca cgccggcgga cttccgctca
     4501 ttggccccga ttgtattgga acaggcattg aaggaatcgg ggacgcagct gctggaacct
     4561 tatctctcct tcatcctcta tgcgccccag gaataccttt ccagggctta tcatgatgca
     4621 ccgaaatact gtgccaccat cgaaacggcc caggtaaaaa aggatgaagt tgtctttact
     4681 ggcgagattc ccgcccgctg tatacaggca taccgtactg atctggcctt ttacaccaac
     4741 gggcggagcg tatgccttac agagctgaaa ggatatcagg ccgctgtcgg tcagccggtc
     4801 atccagcccc gccgtccaaa cagccgcctg gacaaggtgc gccatatgtt tcagaaggta
     4861 atgtaaatcg ttaagggatc aactttggga gagagttcaa aattgatcct ttttttataa
     4921 caggaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat
     4981 gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
     5041 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
     5101 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
     5161 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
     5221 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
     5281 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
     5341 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact
     5401 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
     5461 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
     5521 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
     5581 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
     5641 agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg
     5701 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
     5761 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
     5821 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc
     5881 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
     5941 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
     6001 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
     6061 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
     6121 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     6181 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     6241 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     6301 accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     6361 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     6421 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     6481 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     6541 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     6601 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     6661 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     6721 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cccgagatgc
     6781 gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt
     6841 gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt
     6901 tagcgaggtg ccgccggcat gagctcctct agtagagagc gttcaccgac aaacaacaga
     6961 taaaacgaaa ggcccagtct ttcgactgag cctttcgttt tatttgaaaa agaaaagccc
     7021 cagcaagctt ggcggcagca gggg
//
