LOCUS       sequence-375750-        9330 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  E. coli - Eggerthella lenta shuttle plasmid (KanR), 
            promoterless-lacZ.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9330)
  AUTHORS   Dong X, Guthrie BGH, Alexander M, Noecker C, Ramirez L, Glasser NR, 
            Turnbaugh PJ, Balskus EP
  TITLE     Genetic manipulation of the human gut bacterium Eggerthella lenta 
            reveals a widespread family of transcriptional regulators.
  JOURNAL   Nat Commun. 2022 Dec 9;13(1):7624. doi: 10.1038/s41467-022-33576-3.
  PUBMED    36494336
REFERENCE   2  (bases 1 to 9330)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-12-9"; volume: "13(1):7624. doi"; pages: " 
            10.1038/s41467-022-33576-3"
COMMENT     Sequence Label: pXD70LacZ1-promoterless-LacZ
FEATURES             Location/Qualifiers
     source          1..9330
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      35..106
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     rep_origin      complement(287..875)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(367..386)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1046..1837,1838..1906))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1669..1688
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1907..2011)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2079..2097
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    2254..2273
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that start with ""ATG"""
     CDS             2276..5350
                     /codon_start=1
                     /gene="lacZ"
                     /product="beta-galactosidase"
                     /label=lacZ
                     /translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEART
                     DRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNV
                     TYPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYG
                     QDSRLPSEFDLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQ
                     ISDFHVATRFNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIID
                     ERGGYADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIEN
                     GLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLW
                     YTLCDRYGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWS
                     LGNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVP
                     KWSIKKWLSLPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSL
                     IKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQ
                     TIEVTSEYLFRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQ
                     LWLTVRVVQPNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELG
                     NKRWQFNRQSGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWK
                     AAGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDV
                     EVASDTPHPARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPY
                     VFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDG
                     FHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQK"
     primer_bind     complement(2296..2313)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2296..2312)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2305..2327)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     terminator      5368..5395
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             complement(5518..6312)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
ORIGIN
        1 tgctgccgcc aagcttgctg gggcttttct ttttcaaata aaacgaaagg ctcagtcgaa
       61 agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact agaggagctc
      121 atgccggcgg cacctcgcta acggattcac cactccaaga attggagcca atcaattctt
      181 gcggagaact gtgaatgcgc aaaccaaccc ttggcagaac atatccatcg cgtccgccat
      241 ctccagcagc cgcacgcggc gcatctcggg ccgcgttgct ggcgtttttc cataggctcc
      301 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
      361 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
      421 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
      481 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
      541 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
      601 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
      661 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
      721 ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
      781 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
      841 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
      901 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
      961 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     1021 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     1081 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     1141 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
     1201 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     1261 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     1321 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tgcaggcatc gtggtgtcac
     1381 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     1441 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     1501 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
     1561 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
     1621 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aacacgggat aataccgcgc
     1681 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
     1741 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
     1801 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
     1861 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
     1921 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
     1981 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg
     2041 tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct
     2101 ttcgtcttca aggcgaaaaa accccgccga agcggggttt tttgcgaaat ataatgaccc
     2161 tcttgataac ccaagagggc attttttagg aacggttatt tgattattaa tccggcggca
     2221 actgaaacga ttcggatcct gtattactat tctgaattcg cggccgcttc tagatatgac
     2281 catgattacg gattcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt
     2341 tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga
     2401 ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat ggcgctttgc
     2461 ctggtttccg gcaccagaag cggtgccgga aagctggctg gagtgcgatc ttcctgaggc
     2521 cgatactgtc gtcgtcccct caaactggca gatgcacggt tacgatgcgc ccatctacac
     2581 caacgtgacc tatcccatta cggtcaatcc gccgtttgtt cccacggaga atccgacggg
     2641 ttgttactcg ctcacattta atgttgatga aagctggcta caggaaggcc agacgcgaat
     2701 tatttttgat ggcgttaact cggcgtttca tctgtggtgc aacgggcgct gggtcggtta
     2761 cggccaggac agtcgtttgc cgtctgaatt tgacctgagc gcatttttac gcgccggaga
     2821 aaaccgcctc gcggtgatgg tgctgcgctg gagtgacggc agttatctgg aagatcagga
     2881 tatgtggcgg atgagcggca ttttccgtga cgtctcgttg ctgcataaac cgactacaca
     2941 aatcagcgat ttccatgttg ccactcgctt taatgatgat ttcagccgcg ctgtactgga
     3001 ggctgaagtt cagatgtgcg gcgagttgcg tgactaccta cgggtaacag tttctttatg
     3061 gcagggtgaa acgcaggtcg ccagcggcac cgcgcctttc ggcggtgaaa ttatcgatga
     3121 gcgtggtggt tatgccgatc gcgtcacact acgtctgaac gtcgaaaacc cgaaactgtg
     3181 gagcgccgaa atcccgaatc tctatcgtgc ggtggttgaa ctgcacaccg ccgacggcac
     3241 gctgattgaa gcagaagcct gcgatgtcgg tttccgcgag gtgcggattg aaaatggtct
     3301 gctgctgctg aacggcaagc cgttgctgat tcgaggcgtt aaccgtcacg agcatcatcc
     3361 tctgcatggt caggtcatgg atgagcagac gatggtgcag gatatcctgc tgatgaagca
     3421 gaacaacttt aacgccgtgc gctgttcgca ttatccgaac catccgctgt ggtacacgct
     3481 gtgcgaccgc tacggcctgt atgtggtgga tgaagccaat attgaaaccc acggcatggt
     3541 gccaatgaat cgtctgaccg atgatccgcg ctggctaccg gcgatgagcg aacgcgtaac
     3601 gcgaatggtg cagcgcgatc gtaatcaccc gagtgtgatc atctggtcgc tggggaatga
     3661 atcaggccac ggcgctaatc acgacgcgct gtatcgctgg atcaaatctg tcgatccttc
     3721 ccgcccggtg cagtatgaag gcggcggagc cgacaccacg gccaccgata ttatttgccc
     3781 gatgtacgcg cgcgtggatg aagaccagcc cttcccggct gtgccgaaat ggtccatcaa
     3841 aaaatggctt tcgctacctg gagagacgcg cccgctgatc ctttgcgaat acgcccacgc
     3901 gatgggtaac agtcttggcg gtttcgctaa atactggcag gcgtttcgtc agtatccccg
     3961 tttacagggc ggcttcgtct gggactgggt ggatcagtcg ctgattaaat atgatgaaaa
     4021 cggcaacccg tggtcggctt acggcggtga ttttggcgat acgccgaacg atcgccagtt
     4081 ctgtatgaac ggtctggtct ttgccgaccg cacgccgcat ccagcgctga cggaagcaaa
     4141 acaccagcag cagtttttcc agttccgttt atccgggcaa accatcgaag tgaccagcga
     4201 atacctgttc cgtcatagcg ataacgagct cctgcactgg atggtggcgc tggatggtaa
     4261 gccgctggca agcggtgaag tgcctctgga tgtcgctcca caaggtaaac agttgattga
     4321 actgcctgaa ctaccgcagc cggagagcgc cgggcaactc tggctcacag tacgcgtagt
     4381 gcaaccgaac gcgaccgcat ggtcagaagc cgggcacatc agcgcctggc agcagtggcg
     4441 tctggcggaa aacctcagtg tgacgctccc cgccgcgtcc cacgccatcc cgcatctgac
     4501 caccagcgaa atggattttt gcatcgagct gggtaataag cgttggcaat ttaaccgcca
     4561 gtcaggcttt ctttcacaga tgtggattgg cgataaaaaa caactgctga cgccgctgcg
     4621 cgatcagttc acccgtgcac cgctggataa cgacattggc gtaagtgaag cgacccgcat
     4681 tgaccctaac gcctgggtcg aacgctggaa ggcggcgggc cattaccagg ccgaagcagc
     4741 gttgttgcag tgcacggcag atacacttgc tgatgcggtg ctgattacga ccgctcacgc
     4801 gtggcagcat caggggaaaa ccttatttat cagccggaaa acctaccgga ttgatggtag
     4861 tggtcaaatg gcgattaccg ttgatgttga agtggcgagc gatacaccgc atccggcgcg
     4921 gattggcctg aactgccagc tggcgcaggt agcagagcgg gtaaactggc tcggattagg
     4981 gccgcaagaa aactatcccg accgccttac tgccgcctgt tttgaccgct gggatctgcc
     5041 attgtcagac atgtataccc cgtacgtctt cccgagcgaa aacggtctgc gctgcgggac
     5101 gcgcgaattg aattatggcc cacaccagtg gcgcggcgac ttccagttca acatcagccg
     5161 ctacagtcaa cagcaactga tggaaaccag ccatcgccat ctgctgcacg cggaagaagg
     5221 cacatggctg aatatcgacg gtttccatat ggggattggt ggcgacgact cctggagccc
     5281 gtcagtatcg gcggaattcc agctgagcgc cggtcgctac cattaccagt tggtctggtg
     5341 tcaaaaataa actagtcgac tcacactggc tcaccttcgg gtgggccttt ctgcgtttat
     5401 atactagaga gagaatataa aaagccagat tattaatccg gcttttttat tatttaattc
     5461 ctgttataaa aaaaggatca attttgaact ctctcccaaa gttgatccct taacgatcta
     5521 aaacaattca tccagtaaaa tataatattt tattttctcc caatcaggct tgatccccag
     5581 taagtcaaaa aatagctcga catactgttc ttccccgata tcctccctga tcgaccggac
     5641 gcagaaggca atgtcatacc acttgtccgc cctgccgctt ctcccaagat caataaagcc
     5701 acttactttg ccatctttca caaagatgtt gctgtctccc aggtcgccgt gggaaaagac
     5761 aagttcctct tcgggctttt ccgtctttaa aaaatcatac agctcgcgcg gatctttaaa
     5821 tggagtgtct tcttcccagt tttcgcaatc cacatcggcc agatcgttat tcagtaagta
     5881 atccaattcg gctaagcggc tgtctaagct attcgtatag ggacaatccg atatgtcgat
     5941 ggagtgaaag agcctgatgc actccgcata cagctcgata atcttttcag ggctttgttc
     6001 atcttcatac tcttccgagc aaaggacgcc atcggcctca ctcatgagca gattgctcca
     6061 gccatcatgc cgttcaaagt gcaggacctt tggaacaggc agctttcctt ccagccatag
     6121 catcatgtcc ttttcccgtt ccacatcata ggtggtccct ttataccggc tgtccgtcat
     6181 ttttaaatat aggttttcat tttctcccac cagcttatat accttagcag gagacattcc
     6241 ttccgtatct tttacgcagc ggtatttttc gatcagtttt ttcaattccg gtgatattct
     6301 cattttagcc atttattatt tccttcctct tttctacagt atttaaagat accccaagaa
     6361 gctaattata acaagacgaa ctccaattca ctgttccttg cattctaaaa ccttaaatac
     6421 cagaaaacag ctttttcaaa gttgttttca aagttggcgt ataacatagt atcgacggag
     6481 ccgattttga aaccacaatt atgatagaat ttacaagcta taaggttatt gtcctgggtt
     6541 tcaagcatta gtccatgcaa gtttttatgc tttgcccatt ctatagatat attgataaga
     6601 agactggaat gcccgctccg ccgggattat tcgaaagcgc atcgtccaag cgagaccgtc
     6661 tgcattcgga aatctcggcc ggttcgcgcc gaggaagccc atctggctgc atcccaaatc
     6721 gacacacaac gcacggaggc cccggcgatc gccggggcct ccgaatcatg cgcatcgtgg
     6781 aagcgaagct ccgcaaaaaa ccccgcttca gcggggtttt ttcgcgtcgc acctccttgt
     6841 cgtgtattac aggtactact ataacacatc atctagcccg cccgcgatac gtcctctgcg
     6901 gtgcagcgtg gtcgcggttc cacgcatccg ccgcccgctg cgcatcgcgc agcacgtcat
     6961 taaggctctg ttgccttggc tgttccctgc gcatcagata ttcgagcggg cgcgatattc
     7021 gacttctaag ctcttccact ttgattcgca ttcccgcaac tcctgctctg atgcggctcg
     7081 cgagtccatc gcacttaaca acgatgttat cgaggattgc gccgcgctca gcttttcccg
     7141 cagcttccca gccccgaacc tcggtagcga tgggttgaag ctctccaatg tctcgcgcaa
     7201 ctccgtccgc tcgttgccga agacactcca atcggtcttg ctcatcttcg atatcgagct
     7261 tcacgtcctc gagctggctg tcaagctcgg caaggtcacc ctccttgtcg gcaatctcgg
     7321 taacaagctc tgccttacgc gtttcggcat cattcagtaa agcggcggct tcttccctag
     7381 ccgccgcgac gattcgcgcc gcttcgtcct tcgcaggttc aaccacggcc ttatcgaccg
     7441 cacagcgtac cttgtcgata tccacggact tgtccgtgta tacacgctgc gcccgcgtct
     7501 cctcgtcaag ctcgattgca gggcggtagc ccatgcgccc ctctaggtaa tccccaagct
     7561 ccttgtgcat gctccggtag aagctgcggg ggcacatctg cttgtagttg aaccgtccat
     7621 ctaggatggg cgtgaacggc acgtgcatgt ggtcgcgggc gggcgtgcca tccttcaaca
     7681 cctcgtcctt gtggacgtac ccgcccatca tgttctcgat gccgaacttc ttcgacaggt
     7741 accagtacgt gagacggaag aacctgtcct catcgccctt gcgcacattg tcgggaagcg
     7801 tcacaacaac gtcagccata acgaccgcat ccttgcgcgt ggcacgcttc cctgcggcct
     7861 tctgggcctc gttcacgcgc tcgatgcggc tctccaccgt gccccagttc ggctcgccat
     7921 cgcgcggctc cacgcgcccc acaacgagct tgccgtcctt gttcgtgtag tggccgacca
     7981 ccaagtcgtt cttgatgcgc tcggggtcga tgttgtcgcg ctccaggctg ctgcggtcgc
     8041 ggcggtagtg ggcaagcatg tgcccgaccg atggagcctt gtatttcgcg atgtgagcca
     8101 tggaaacctc cgaaccgtac agacttaatc gactaaagtc tagcacgtta gaccttagct
     8161 acgctaagga cgtggcaggg ccagccctgc acccgctcgg gcgttccccc cgagaccccc
     8221 cagcccttgc ccgttcgcgg cttcgctcac ggccctcggg ccatcggcgc gagcgccgaa
     8281 cggagggact tcgcccctcc gaaccacccc tcaatctacc tgcggttcgg cacacggcac
     8341 acggcactat taggggggcc gtcgtagtgg cccccctgtg gtcatttgtc atttgtcatt
     8401 tgtcacatta tttttcggac acattatttt tcggacgtag ttagcgaaaa ataatgagcc
     8461 gcccgattct gcaggtctca tccagtcgag acacgggcaa ggaaagggtc gatggagcag
     8521 agcgtccatc gacccttaat caagtctggt ctattcggtt ggccgtcgcg tcaattccgc
     8581 aatccgtgaa cgagtcggct tagatctgcg cgagcgcaga ccacgccgcc ccgtagtcac
     8641 gcattgctgc attgacccga ctactctttg gcacctgact gttccacgca cttgcgctta
     8701 cgttcggcgt tcagatagtt cccgcgacaa tagcgctcga gaatcgagtt gtaccccacg
     8761 atgatttcgc ggtactgctc gggaaactcc ctcagcgccg cagccgtgaa atcgttgatg
     8821 gtctcgaacc tctcgcacat gatgaagccg accaactcga agagcatgtc ctgcttgtcc
     8881 ttagtcgaga gctgttcgag ctgaccaatg tcgaagttgt ttccctcgat gcgtgcttcg
     8941 accggataca gctccttgcc cttcttgcga caggaagccg tgtcgtgaat cagatagtca
     9001 tacgcatgcc tgatgttgtt gattggctcg gctgacgaac agcacttctt gccgtccgcg
     9061 ctcaatcggt tcaagacgtt gatgattgcc ttgcgtgtcg tgttgccgcc ccagacaaca
     9121 atgacgtgcg catgcgtctt gcgctgcttg ctcttctggt catggtctat gtcgtgaacc
     9181 gcataagcga aaggcacttg aagaatgtcg tctatctcgt cctgccagtc atcgcgcatg
     9241 ttctctgggt agcagatgcc ctcccagagc ttcaccttcg tctcgccagc gtttgccgcc
     9301 atgctataat ctccttgtct cgaatgcccc
//
