LOCUS       sequence-375754-        9717 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  E. coli - Eggerthella lenta shuttle plasmid (KanR), Pdadh dopamine 
            dehydroxylase promoter-lacZ.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9717)
  AUTHORS   Dong X, Guthrie BGH, Alexander M, Noecker C, Ramirez L, Glasser NR, 
            Turnbaugh PJ, Balskus EP
  TITLE     Genetic manipulation of the human gut bacterium Eggerthella lenta 
            reveals a widespread family of transcriptional regulators.
  JOURNAL   Nat Commun. 2022 Dec 9;13(1):7624. doi: 10.1038/s41467-022-33576-3.
  PUBMED    36494336
REFERENCE   2  (bases 1 to 9717)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-12-9"; volume: "13(1):7624. doi"; pages: " 
            10.1038/s41467-022-33576-3"
COMMENT     Sequence Label: pXD70LacZ2-Pdadh-LacZ
FEATURES             Location/Qualifiers
     source          1..9717
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      41..112
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     rep_origin      complement(293..881)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(373..392)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1052..1843,1844..1912))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1675..1694
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1913..2017)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2085..2103
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    2260..2279
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that start with ""ATG"""
     CDS             2669..5743
                     /codon_start=1
                     /gene="lacZ"
                     /product="beta-galactosidase"
                     /label=lacZ
                     /translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEART
                     DRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNV
                     TYPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYG
                     QDSRLPSEFDLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQ
                     ISDFHVATRFNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIID
                     ERGGYADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIEN
                     GLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLW
                     YTLCDRYGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWS
                     LGNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVP
                     KWSIKKWLSLPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSL
                     IKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQ
                     TIEVTSEYLFRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQ
                     LWLTVRVVQPNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELG
                     NKRWQFNRQSGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWK
                     AAGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDV
                     EVASDTPHPARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPY
                     VFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDG
                     FHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQK"
     primer_bind     complement(2689..2706)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2689..2705)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2698..2720)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     terminator      5761..5788
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             complement(5911..6705)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
ORIGIN
        1 tgcccctgct gccgccaagc ttgctggggc ttttcttttt caaataaaac gaaaggctca
       61 gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag
      121 gagctcatgc cggcggcacc tcgctaacgg attcaccact ccaagaattg gagccaatca
      181 attcttgcgg agaactgtga atgcgcaaac caacccttgg cagaacatat ccatcgcgtc
      241 cgccatctcc agcagccgca cgcggcgcat ctcgggccgc gttgctggcg tttttccata
      301 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
      361 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
      421 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
      481 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
      541 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
      601 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
      661 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
      721 gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
      781 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
      841 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
      901 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
      961 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     1021 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     1081 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     1141 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
     1201 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     1261 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     1321 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctgca ggcatcgtgg
     1381 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     1441 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     1501 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     1561 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
     1621 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaaca cgggataata
     1681 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
     1741 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
     1801 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
     1861 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
     1921 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
     1981 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
     2041 ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga
     2101 ggccctttcg tcttcaaggc gaaaaaaccc cgccgaagcg gggttttttg cgaaatataa
     2161 tgaccctctt gataacccaa gagggcattt tttaggaacg gttatttgat tattaatccg
     2221 gcggcaactg aaacgattcg gatcctgtat tactattctg aattcgcggc cgcttctaga
     2281 tgccccaccc cctgtgagcc cgtacataac gtcgctcgcg ccgacccctc tgtcgacggg
     2341 ttcgattata cggaaaagat gagcggccct ctttgaggaa actattctag tttcagaata
     2401 atttatgacc tacggttaaa gtttccccag ttcaaagcac tatcgaattt ttcatgatca
     2461 caagatcata agaatttatg atgcggtcga acctgctttc caacctttgg acgatgctct
     2521 gcaccccctc gcatccgtat ggtcggccca agcgacacgg ggactttgca ccggttcgga
     2581 gcccgaggct tcacccggca gcccccctcc ccgatcgcac gaacgaagac gagcaagacg
     2641 agaacgagga gaaagaagga ggaggagtat gaccatgatt acggattcac tggccgtcgt
     2701 tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca
     2761 tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca
     2821 gttgcgcagc ctgaatggcg aatggcgctt tgcctggttt ccggcaccag aagcggtgcc
     2881 ggaaagctgg ctggagtgcg atcttcctga ggccgatact gtcgtcgtcc cctcaaactg
     2941 gcagatgcac ggttacgatg cgcccatcta caccaacgtg acctatccca ttacggtcaa
     3001 tccgccgttt gttcccacgg agaatccgac gggttgttac tcgctcacat ttaatgttga
     3061 tgaaagctgg ctacaggaag gccagacgcg aattattttt gatggcgtta actcggcgtt
     3121 tcatctgtgg tgcaacgggc gctgggtcgg ttacggccag gacagtcgtt tgccgtctga
     3181 atttgacctg agcgcatttt tacgcgccgg agaaaaccgc ctcgcggtga tggtgctgcg
     3241 ctggagtgac ggcagttatc tggaagatca ggatatgtgg cggatgagcg gcattttccg
     3301 tgacgtctcg ttgctgcata aaccgactac acaaatcagc gatttccatg ttgccactcg
     3361 ctttaatgat gatttcagcc gcgctgtact ggaggctgaa gttcagatgt gcggcgagtt
     3421 gcgtgactac ctacgggtaa cagtttcttt atggcagggt gaaacgcagg tcgccagcgg
     3481 caccgcgcct ttcggcggtg aaattatcga tgagcgtggt ggttatgccg atcgcgtcac
     3541 actacgtctg aacgtcgaaa acccgaaact gtggagcgcc gaaatcccga atctctatcg
     3601 tgcggtggtt gaactgcaca ccgccgacgg cacgctgatt gaagcagaag cctgcgatgt
     3661 cggtttccgc gaggtgcgga ttgaaaatgg tctgctgctg ctgaacggca agccgttgct
     3721 gattcgaggc gttaaccgtc acgagcatca tcctctgcat ggtcaggtca tggatgagca
     3781 gacgatggtg caggatatcc tgctgatgaa gcagaacaac tttaacgccg tgcgctgttc
     3841 gcattatccg aaccatccgc tgtggtacac gctgtgcgac cgctacggcc tgtatgtggt
     3901 ggatgaagcc aatattgaaa cccacggcat ggtgccaatg aatcgtctga ccgatgatcc
     3961 gcgctggcta ccggcgatga gcgaacgcgt aacgcgaatg gtgcagcgcg atcgtaatca
     4021 cccgagtgtg atcatctggt cgctggggaa tgaatcaggc cacggcgcta atcacgacgc
     4081 gctgtatcgc tggatcaaat ctgtcgatcc ttcccgcccg gtgcagtatg aaggcggcgg
     4141 agccgacacc acggccaccg atattatttg cccgatgtac gcgcgcgtgg atgaagacca
     4201 gcccttcccg gctgtgccga aatggtccat caaaaaatgg ctttcgctac ctggagagac
     4261 gcgcccgctg atcctttgcg aatacgccca cgcgatgggt aacagtcttg gcggtttcgc
     4321 taaatactgg caggcgtttc gtcagtatcc ccgtttacag ggcggcttcg tctgggactg
     4381 ggtggatcag tcgctgatta aatatgatga aaacggcaac ccgtggtcgg cttacggcgg
     4441 tgattttggc gatacgccga acgatcgcca gttctgtatg aacggtctgg tctttgccga
     4501 ccgcacgccg catccagcgc tgacggaagc aaaacaccag cagcagtttt tccagttccg
     4561 tttatccggg caaaccatcg aagtgaccag cgaatacctg ttccgtcata gcgataacga
     4621 gctcctgcac tggatggtgg cgctggatgg taagccgctg gcaagcggtg aagtgcctct
     4681 ggatgtcgct ccacaaggta aacagttgat tgaactgcct gaactaccgc agccggagag
     4741 cgccgggcaa ctctggctca cagtacgcgt agtgcaaccg aacgcgaccg catggtcaga
     4801 agccgggcac atcagcgcct ggcagcagtg gcgtctggcg gaaaacctca gtgtgacgct
     4861 ccccgccgcg tcccacgcca tcccgcatct gaccaccagc gaaatggatt tttgcatcga
     4921 gctgggtaat aagcgttggc aatttaaccg ccagtcaggc tttctttcac agatgtggat
     4981 tggcgataaa aaacaactgc tgacgccgct gcgcgatcag ttcacccgtg caccgctgga
     5041 taacgacatt ggcgtaagtg aagcgacccg cattgaccct aacgcctggg tcgaacgctg
     5101 gaaggcggcg ggccattacc aggccgaagc agcgttgttg cagtgcacgg cagatacact
     5161 tgctgatgcg gtgctgatta cgaccgctca cgcgtggcag catcagggga aaaccttatt
     5221 tatcagccgg aaaacctacc ggattgatgg tagtggtcaa atggcgatta ccgttgatgt
     5281 tgaagtggcg agcgatacac cgcatccggc gcggattggc ctgaactgcc agctggcgca
     5341 ggtagcagag cgggtaaact ggctcggatt agggccgcaa gaaaactatc ccgaccgcct
     5401 tactgccgcc tgttttgacc gctgggatct gccattgtca gacatgtata ccccgtacgt
     5461 cttcccgagc gaaaacggtc tgcgctgcgg gacgcgcgaa ttgaattatg gcccacacca
     5521 gtggcgcggc gacttccagt tcaacatcag ccgctacagt caacagcaac tgatggaaac
     5581 cagccatcgc catctgctgc acgcggaaga aggcacatgg ctgaatatcg acggtttcca
     5641 tatggggatt ggtggcgacg actcctggag cccgtcagta tcggcggaat tccagctgag
     5701 cgccggtcgc taccattacc agttggtctg gtgtcaaaaa taaactagtc gactcacact
     5761 ggctcacctt cgggtgggcc tttctgcgtt tatatactag agagagaata taaaaagcca
     5821 gattattaat ccggcttttt tattatttaa ttcctgttat aaaaaaagga tcaattttga
     5881 actctctccc aaagttgatc ccttaacgat ctaaaacaat tcatccagta aaatataata
     5941 ttttattttc tcccaatcag gcttgatccc cagtaagtca aaaaatagct cgacatactg
     6001 ttcttccccg atatcctccc tgatcgaccg gacgcagaag gcaatgtcat accacttgtc
     6061 cgccctgccg cttctcccaa gatcaataaa gccacttact ttgccatctt tcacaaagat
     6121 gttgctgtct cccaggtcgc cgtgggaaaa gacaagttcc tcttcgggct tttccgtctt
     6181 taaaaaatca tacagctcgc gcggatcttt aaatggagtg tcttcttccc agttttcgca
     6241 atccacatcg gccagatcgt tattcagtaa gtaatccaat tcggctaagc ggctgtctaa
     6301 gctattcgta tagggacaat ccgatatgtc gatggagtga aagagcctga tgcactccgc
     6361 atacagctcg ataatctttt cagggctttg ttcatcttca tactcttccg agcaaaggac
     6421 gccatcggcc tcactcatga gcagattgct ccagccatca tgccgttcaa agtgcaggac
     6481 ctttggaaca ggcagctttc cttccagcca tagcatcatg tccttttccc gttccacatc
     6541 ataggtggtc cctttatacc ggctgtccgt catttttaaa tataggtttt cattttctcc
     6601 caccagctta tataccttag caggagacat tccttccgta tcttttacgc agcggtattt
     6661 ttcgatcagt tttttcaatt ccggtgatat tctcatttta gccatttatt atttccttcc
     6721 tcttttctac agtatttaaa gataccccaa gaagctaatt ataacaagac gaactccaat
     6781 tcactgttcc ttgcattcta aaaccttaaa taccagaaaa cagctttttc aaagttgttt
     6841 tcaaagttgg cgtataacat agtatcgacg gagccgattt tgaaaccaca attatgatag
     6901 aatttacaag ctataaggtt attgtcctgg gtttcaagca ttagtccatg caagttttta
     6961 tgctttgccc attctataga tatattgata agaagactgg aatgcccgct ccgccgggat
     7021 tattcgaaag cgcatcgtcc aagcgagacc gtctgcattc ggaaatctcg gccggttcgc
     7081 gccgaggaag cccatctggc tgcatcccaa atcgacacac aacgcacgga ggccccggcg
     7141 atcgccgggg cctccgaatc atgcgcatcg tggaagcgaa gctccgcaaa aaaccccgct
     7201 tcagcggggt tttttcgcgt cgcacctcct tgtcgtgtat tacaggtact actataacac
     7261 atcatctagc ccgcccgcga tacgtcctct gcggtgcagc gtggtcgcgg ttccacgcat
     7321 ccgccgcccg ctgcgcatcg cgcagcacgt cattaaggct ctgttgcctt ggctgttccc
     7381 tgcgcatcag atattcgagc gggcgcgata ttcgacttct aagctcttcc actttgattc
     7441 gcattcccgc aactcctgct ctgatgcggc tcgcgagtcc atcgcactta acaacgatgt
     7501 tatcgaggat tgcgccgcgc tcagcttttc ccgcagcttc ccagccccga acctcggtag
     7561 cgatgggttg aagctctcca atgtctcgcg caactccgtc cgctcgttgc cgaagacact
     7621 ccaatcggtc ttgctcatct tcgatatcga gcttcacgtc ctcgagctgg ctgtcaagct
     7681 cggcaaggtc accctccttg tcggcaatct cggtaacaag ctctgcctta cgcgtttcgg
     7741 catcattcag taaagcggcg gcttcttccc tagccgccgc gacgattcgc gccgcttcgt
     7801 ccttcgcagg ttcaaccacg gccttatcga ccgcacagcg taccttgtcg atatccacgg
     7861 acttgtccgt gtatacacgc tgcgcccgcg tctcctcgtc aagctcgatt gcagggcggt
     7921 agcccatgcg cccctctagg taatccccaa gctccttgtg catgctccgg tagaagctgc
     7981 gggggcacat ctgcttgtag ttgaaccgtc catctaggat gggcgtgaac ggcacgtgca
     8041 tgtggtcgcg ggcgggcgtg ccatccttca acacctcgtc cttgtggacg tacccgccca
     8101 tcatgttctc gatgccgaac ttcttcgaca ggtaccagta cgtgagacgg aagaacctgt
     8161 cctcatcgcc cttgcgcaca ttgtcgggaa gcgtcacaac aacgtcagcc ataacgaccg
     8221 catccttgcg cgtggcacgc ttccctgcgg ccttctgggc ctcgttcacg cgctcgatgc
     8281 ggctctccac cgtgccccag ttcggctcgc catcgcgcgg ctccacgcgc cccacaacga
     8341 gcttgccgtc cttgttcgtg tagtggccga ccaccaagtc gttcttgatg cgctcggggt
     8401 cgatgttgtc gcgctccagg ctgctgcggt cgcggcggta gtgggcaagc atgtgcccga
     8461 ccgatggagc cttgtatttc gcgatgtgag ccatggaaac ctccgaaccg tacagactta
     8521 atcgactaaa gtctagcacg ttagacctta gctacgctaa ggacgtggca gggccagccc
     8581 tgcacccgct cgggcgttcc ccccgagacc ccccagccct tgcccgttcg cggcttcgct
     8641 cacggccctc gggccatcgg cgcgagcgcc gaacggaggg acttcgcccc tccgaaccac
     8701 ccctcaatct acctgcggtt cggcacacgg cacacggcac tattaggggg gccgtcgtag
     8761 tggcccccct gtggtcattt gtcatttgtc atttgtcaca ttatttttcg gacacattat
     8821 ttttcggacg tagttagcga aaaataatga gccgcccgat tctgcaggtc tcatccagtc
     8881 gagacacggg caaggaaagg gtcgatggag cagagcgtcc atcgaccctt aatcaagtct
     8941 ggtctattcg gttggccgtc gcgtcaattc cgcaatccgt gaacgagtcg gcttagatct
     9001 gcgcgagcgc agaccacgcc gccccgtagt cacgcattgc tgcattgacc cgactactct
     9061 ttggcacctg actgttccac gcacttgcgc ttacgttcgg cgttcagata gttcccgcga
     9121 caatagcgct cgagaatcga gttgtacccc acgatgattt cgcggtactg ctcgggaaac
     9181 tccctcagcg ccgcagccgt gaaatcgttg atggtctcga acctctcgca catgatgaag
     9241 ccgaccaact cgaagagcat gtcctgcttg tccttagtcg agagctgttc gagctgacca
     9301 atgtcgaagt tgtttccctc gatgcgtgct tcgaccggat acagctcctt gcccttcttg
     9361 cgacaggaag ccgtgtcgtg aatcagatag tcatacgcat gcctgatgtt gttgattggc
     9421 tcggctgacg aacagcactt cttgccgtcc gcgctcaatc ggttcaagac gttgatgatt
     9481 gccttgcgtg tcgtgttgcc gccccagaca acaatgacgt gcgcatgcgt cttgcgctgc
     9541 ttgctcttct ggtcatggtc tatgtcgtga accgcataag cgaaaggcac ttgaagaatg
     9601 tcgtctatct cgtcctgcca gtcatcgcgc atgttctctg ggtagcagat gccctcccag
     9661 agcttcacct tcgtctcgcc agcgtttgcc gccatgctat aatctccttg tctcgaa
//
