LOCUS       sequence-375755-        9503 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  E. coli - Eggerthella lenta shuttle plasmid (KanR), Pcsd 
            promoter-lacZ, E. lenta constitutive promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9503)
  AUTHORS   Dong X, Guthrie BGH, Alexander M, Noecker C, Ramirez L, Glasser NR, 
            Turnbaugh PJ, Balskus EP
  TITLE     Genetic manipulation of the human gut bacterium Eggerthella lenta 
            reveals a widespread family of transcriptional regulators.
  JOURNAL   Nat Commun. 2022 Dec 9;13(1):7624. doi: 10.1038/s41467-022-33576-3.
  PUBMED    36494336
REFERENCE   2  (bases 1 to 9503)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-12-9"; volume: "13(1):7624. doi"; pages: " 
            10.1038/s41467-022-33576-3"
COMMENT     Sequence Label: pXD70LacZ3-Pcsd-LacZ
FEATURES             Location/Qualifiers
     source          1..9503
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      23..94
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     rep_origin      complement(275..863)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(355..374)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1034..1825,1826..1894))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1657..1676
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1895..1999)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2067..2085
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    2242..2261
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that start with ""ATG"""
     CDS             2437..5511
                     /codon_start=1
                     /gene="lacZ"
                     /product="beta-galactosidase"
                     /label=lacZ
                     /translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEART
                     DRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNV
                     TYPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYG
                     QDSRLPSEFDLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQ
                     ISDFHVATRFNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIID
                     ERGGYADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIEN
                     GLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLW
                     YTLCDRYGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWS
                     LGNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVP
                     KWSIKKWLSLPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSL
                     IKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQ
                     TIEVTSEYLFRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQ
                     LWLTVRVVQPNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELG
                     NKRWQFNRQSGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWK
                     AAGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDV
                     EVASDTPHPARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPY
                     VFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDG
                     FHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQK"
     primer_bind     complement(2457..2474)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2457..2473)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2466..2488)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     terminator      5529..5556
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             complement(5679..6473)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
ORIGIN
        1 gcttgctggg gcttttcttt ttcaaataaa acgaaaggct cagtcgaaag actgggcctt
       61 tcgttttatc tgttgtttgt cggtgaacgc tctctactag aggagctcat gccggcggca
      121 cctcgctaac ggattcacca ctccaagaat tggagccaat caattcttgc ggagaactgt
      181 gaatgcgcaa accaaccctt ggcagaacat atccatcgcg tccgccatct ccagcagccg
      241 cacgcggcgc atctcgggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
      301 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
      361 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
      421 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
      481 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
      541 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
      601 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
      661 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag
      721 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
      781 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
      841 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
      901 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
      961 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     1021 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     1081 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     1141 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     1201 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     1261 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     1321 atagtttgcg caacgttgtt gccattgctg caggcatcgt ggtgtcacgc tcgtcgtttg
     1381 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     1441 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     1501 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     1561 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     1621 ggcgaccgag ttgctcttgc ccggcgtcaa cacgggataa taccgcgcca catagcagaa
     1681 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     1741 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     1801 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     1861 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     1921 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     1981 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca
     2041 ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtcttcaag
     2101 gcgaaaaaac cccgccgaag cggggttttt tgcgaaatat aatgaccctc ttgataaccc
     2161 aagagggcat tttttaggaa cggttatttg attattaatc cggcggcaac tgaaacgatt
     2221 cggatcctgt attactattc tgaattcgcg gccgcttcta gatgagcccg ctcgatcaac
     2281 ggatcggtaa aatacgggcg aaaaagagaa tcccgagctt ttttgttgcc gcatagcgta
     2341 tagtaagcat gtgccctcga ggtgagggcg agtgttgcgg ccatgcagca cacagtgtta
     2401 cctcctcggg gacgaggaga ggaaagagga tttaaaatga ccatgattac ggattcactg
     2461 gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt
     2521 gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct
     2581 tcccaacagt tgcgcagcct gaatggcgaa tggcgctttg cctggtttcc ggcaccagaa
     2641 gcggtgccgg aaagctggct ggagtgcgat cttcctgagg ccgatactgt cgtcgtcccc
     2701 tcaaactggc agatgcacgg ttacgatgcg cccatctaca ccaacgtgac ctatcccatt
     2761 acggtcaatc cgccgtttgt tcccacggag aatccgacgg gttgttactc gctcacattt
     2821 aatgttgatg aaagctggct acaggaaggc cagacgcgaa ttatttttga tggcgttaac
     2881 tcggcgtttc atctgtggtg caacgggcgc tgggtcggtt acggccagga cagtcgtttg
     2941 ccgtctgaat ttgacctgag cgcattttta cgcgccggag aaaaccgcct cgcggtgatg
     3001 gtgctgcgct ggagtgacgg cagttatctg gaagatcagg atatgtggcg gatgagcggc
     3061 attttccgtg acgtctcgtt gctgcataaa ccgactacac aaatcagcga tttccatgtt
     3121 gccactcgct ttaatgatga tttcagccgc gctgtactgg aggctgaagt tcagatgtgc
     3181 ggcgagttgc gtgactacct acgggtaaca gtttctttat ggcagggtga aacgcaggtc
     3241 gccagcggca ccgcgccttt cggcggtgaa attatcgatg agcgtggtgg ttatgccgat
     3301 cgcgtcacac tacgtctgaa cgtcgaaaac ccgaaactgt ggagcgccga aatcccgaat
     3361 ctctatcgtg cggtggttga actgcacacc gccgacggca cgctgattga agcagaagcc
     3421 tgcgatgtcg gtttccgcga ggtgcggatt gaaaatggtc tgctgctgct gaacggcaag
     3481 ccgttgctga ttcgaggcgt taaccgtcac gagcatcatc ctctgcatgg tcaggtcatg
     3541 gatgagcaga cgatggtgca ggatatcctg ctgatgaagc agaacaactt taacgccgtg
     3601 cgctgttcgc attatccgaa ccatccgctg tggtacacgc tgtgcgaccg ctacggcctg
     3661 tatgtggtgg atgaagccaa tattgaaacc cacggcatgg tgccaatgaa tcgtctgacc
     3721 gatgatccgc gctggctacc ggcgatgagc gaacgcgtaa cgcgaatggt gcagcgcgat
     3781 cgtaatcacc cgagtgtgat catctggtcg ctggggaatg aatcaggcca cggcgctaat
     3841 cacgacgcgc tgtatcgctg gatcaaatct gtcgatcctt cccgcccggt gcagtatgaa
     3901 ggcggcggag ccgacaccac ggccaccgat attatttgcc cgatgtacgc gcgcgtggat
     3961 gaagaccagc ccttcccggc tgtgccgaaa tggtccatca aaaaatggct ttcgctacct
     4021 ggagagacgc gcccgctgat cctttgcgaa tacgcccacg cgatgggtaa cagtcttggc
     4081 ggtttcgcta aatactggca ggcgtttcgt cagtatcccc gtttacaggg cggcttcgtc
     4141 tgggactggg tggatcagtc gctgattaaa tatgatgaaa acggcaaccc gtggtcggct
     4201 tacggcggtg attttggcga tacgccgaac gatcgccagt tctgtatgaa cggtctggtc
     4261 tttgccgacc gcacgccgca tccagcgctg acggaagcaa aacaccagca gcagtttttc
     4321 cagttccgtt tatccgggca aaccatcgaa gtgaccagcg aatacctgtt ccgtcatagc
     4381 gataacgagc tcctgcactg gatggtggcg ctggatggta agccgctggc aagcggtgaa
     4441 gtgcctctgg atgtcgctcc acaaggtaaa cagttgattg aactgcctga actaccgcag
     4501 ccggagagcg ccgggcaact ctggctcaca gtacgcgtag tgcaaccgaa cgcgaccgca
     4561 tggtcagaag ccgggcacat cagcgcctgg cagcagtggc gtctggcgga aaacctcagt
     4621 gtgacgctcc ccgccgcgtc ccacgccatc ccgcatctga ccaccagcga aatggatttt
     4681 tgcatcgagc tgggtaataa gcgttggcaa tttaaccgcc agtcaggctt tctttcacag
     4741 atgtggattg gcgataaaaa acaactgctg acgccgctgc gcgatcagtt cacccgtgca
     4801 ccgctggata acgacattgg cgtaagtgaa gcgacccgca ttgaccctaa cgcctgggtc
     4861 gaacgctgga aggcggcggg ccattaccag gccgaagcag cgttgttgca gtgcacggca
     4921 gatacacttg ctgatgcggt gctgattacg accgctcacg cgtggcagca tcaggggaaa
     4981 accttattta tcagccggaa aacctaccgg attgatggta gtggtcaaat ggcgattacc
     5041 gttgatgttg aagtggcgag cgatacaccg catccggcgc ggattggcct gaactgccag
     5101 ctggcgcagg tagcagagcg ggtaaactgg ctcggattag ggccgcaaga aaactatccc
     5161 gaccgcctta ctgccgcctg ttttgaccgc tgggatctgc cattgtcaga catgtatacc
     5221 ccgtacgtct tcccgagcga aaacggtctg cgctgcggga cgcgcgaatt gaattatggc
     5281 ccacaccagt ggcgcggcga cttccagttc aacatcagcc gctacagtca acagcaactg
     5341 atggaaacca gccatcgcca tctgctgcac gcggaagaag gcacatggct gaatatcgac
     5401 ggtttccata tggggattgg tggcgacgac tcctggagcc cgtcagtatc ggcggaattc
     5461 cagctgagcg ccggtcgcta ccattaccag ttggtctggt gtcaaaaata aactagtcga
     5521 ctcacactgg ctcaccttcg ggtgggcctt tctgcgttta tatactagag agagaatata
     5581 aaaagccaga ttattaatcc ggctttttta ttatttaatt cctgttataa aaaaaggatc
     5641 aattttgaac tctctcccaa agttgatccc ttaacgatct aaaacaattc atccagtaaa
     5701 atataatatt ttattttctc ccaatcaggc ttgatcccca gtaagtcaaa aaatagctcg
     5761 acatactgtt cttccccgat atcctccctg atcgaccgga cgcagaaggc aatgtcatac
     5821 cacttgtccg ccctgccgct tctcccaaga tcaataaagc cacttacttt gccatctttc
     5881 acaaagatgt tgctgtctcc caggtcgccg tgggaaaaga caagttcctc ttcgggcttt
     5941 tccgtcttta aaaaatcata cagctcgcgc ggatctttaa atggagtgtc ttcttcccag
     6001 ttttcgcaat ccacatcggc cagatcgtta ttcagtaagt aatccaattc ggctaagcgg
     6061 ctgtctaagc tattcgtata gggacaatcc gatatgtcga tggagtgaaa gagcctgatg
     6121 cactccgcat acagctcgat aatcttttca gggctttgtt catcttcata ctcttccgag
     6181 caaaggacgc catcggcctc actcatgagc agattgctcc agccatcatg ccgttcaaag
     6241 tgcaggacct ttggaacagg cagctttcct tccagccata gcatcatgtc cttttcccgt
     6301 tccacatcat aggtggtccc tttataccgg ctgtccgtca tttttaaata taggttttca
     6361 ttttctccca ccagcttata taccttagca ggagacattc cttccgtatc ttttacgcag
     6421 cggtattttt cgatcagttt tttcaattcc ggtgatattc tcattttagc catttattat
     6481 ttccttcctc ttttctacag tatttaaaga taccccaaga agctaattat aacaagacga
     6541 actccaattc actgttcctt gcattctaaa accttaaata ccagaaaaca gctttttcaa
     6601 agttgttttc aaagttggcg tataacatag tatcgacgga gccgattttg aaaccacaat
     6661 tatgatagaa tttacaagct ataaggttat tgtcctgggt ttcaagcatt agtccatgca
     6721 agtttttatg ctttgcccat tctatagata tattgataag aagactggaa tgcccgctcc
     6781 gccgggatta ttcgaaagcg catcgtccaa gcgagaccgt ctgcattcgg aaatctcggc
     6841 cggttcgcgc cgaggaagcc catctggctg catcccaaat cgacacacaa cgcacggagg
     6901 ccccggcgat cgccggggcc tccgaatcat gcgcatcgtg gaagcgaagc tccgcaaaaa
     6961 accccgcttc agcggggttt tttcgcgtcg cacctccttg tcgtgtatta caggtactac
     7021 tataacacat catctagccc gcccgcgata cgtcctctgc ggtgcagcgt ggtcgcggtt
     7081 ccacgcatcc gccgcccgct gcgcatcgcg cagcacgtca ttaaggctct gttgccttgg
     7141 ctgttccctg cgcatcagat attcgagcgg gcgcgatatt cgacttctaa gctcttccac
     7201 tttgattcgc attcccgcaa ctcctgctct gatgcggctc gcgagtccat cgcacttaac
     7261 aacgatgtta tcgaggattg cgccgcgctc agcttttccc gcagcttccc agccccgaac
     7321 ctcggtagcg atgggttgaa gctctccaat gtctcgcgca actccgtccg ctcgttgccg
     7381 aagacactcc aatcggtctt gctcatcttc gatatcgagc ttcacgtcct cgagctggct
     7441 gtcaagctcg gcaaggtcac cctccttgtc ggcaatctcg gtaacaagct ctgccttacg
     7501 cgtttcggca tcattcagta aagcggcggc ttcttcccta gccgccgcga cgattcgcgc
     7561 cgcttcgtcc ttcgcaggtt caaccacggc cttatcgacc gcacagcgta ccttgtcgat
     7621 atccacggac ttgtccgtgt atacacgctg cgcccgcgtc tcctcgtcaa gctcgattgc
     7681 agggcggtag cccatgcgcc cctctaggta atccccaagc tccttgtgca tgctccggta
     7741 gaagctgcgg gggcacatct gcttgtagtt gaaccgtcca tctaggatgg gcgtgaacgg
     7801 cacgtgcatg tggtcgcggg cgggcgtgcc atccttcaac acctcgtcct tgtggacgta
     7861 cccgcccatc atgttctcga tgccgaactt cttcgacagg taccagtacg tgagacggaa
     7921 gaacctgtcc tcatcgccct tgcgcacatt gtcgggaagc gtcacaacaa cgtcagccat
     7981 aacgaccgca tccttgcgcg tggcacgctt ccctgcggcc ttctgggcct cgttcacgcg
     8041 ctcgatgcgg ctctccaccg tgccccagtt cggctcgcca tcgcgcggct ccacgcgccc
     8101 cacaacgagc ttgccgtcct tgttcgtgta gtggccgacc accaagtcgt tcttgatgcg
     8161 ctcggggtcg atgttgtcgc gctccaggct gctgcggtcg cggcggtagt gggcaagcat
     8221 gtgcccgacc gatggagcct tgtatttcgc gatgtgagcc atggaaacct ccgaaccgta
     8281 cagacttaat cgactaaagt ctagcacgtt agaccttagc tacgctaagg acgtggcagg
     8341 gccagccctg cacccgctcg ggcgttcccc ccgagacccc ccagcccttg cccgttcgcg
     8401 gcttcgctca cggccctcgg gccatcggcg cgagcgccga acggagggac ttcgcccctc
     8461 cgaaccaccc ctcaatctac ctgcggttcg gcacacggca cacggcacta ttaggggggc
     8521 cgtcgtagtg gcccccctgt ggtcatttgt catttgtcat ttgtcacatt atttttcgga
     8581 cacattattt ttcggacgta gttagcgaaa aataatgagc cgcccgattc tgcaggtctc
     8641 atccagtcga gacacgggca aggaaagggt cgatggagca gagcgtccat cgacccttaa
     8701 tcaagtctgg tctattcggt tggccgtcgc gtcaattccg caatccgtga acgagtcggc
     8761 ttagatctgc gcgagcgcag accacgccgc cccgtagtca cgcattgctg cattgacccg
     8821 actactcttt ggcacctgac tgttccacgc acttgcgctt acgttcggcg ttcagatagt
     8881 tcccgcgaca atagcgctcg agaatcgagt tgtaccccac gatgatttcg cggtactgct
     8941 cgggaaactc cctcagcgcc gcagccgtga aatcgttgat ggtctcgaac ctctcgcaca
     9001 tgatgaagcc gaccaactcg aagagcatgt cctgcttgtc cttagtcgag agctgttcga
     9061 gctgaccaat gtcgaagttg tttccctcga tgcgtgcttc gaccggatac agctccttgc
     9121 ccttcttgcg acaggaagcc gtgtcgtgaa tcagatagtc atacgcatgc ctgatgttgt
     9181 tgattggctc ggctgacgaa cagcacttct tgccgtccgc gctcaatcgg ttcaagacgt
     9241 tgatgattgc cttgcgtgtc gtgttgccgc cccagacaac aatgacgtgc gcatgcgtct
     9301 tgcgctgctt gctcttctgg tcatggtcta tgtcgtgaac cgcataagcg aaaggcactt
     9361 gaagaatgtc gtctatctcg tcctgccagt catcgcgcat gttctctggg tagcagatgc
     9421 cctcccagag cttcaccttc gtctcgccag cgtttgccgc catgctataa tctccttgtc
     9481 tcgaatgccc ctgctgccgc caa
//
