LOCUS       sequence-375757-        9496 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  E. coli - Eggerthella lenta shuttle plasmid (KanR), PdegV 
            promoter-lacZ, E. lenta constitutive promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9496)
  AUTHORS   Dong X, Guthrie BGH, Alexander M, Noecker C, Ramirez L, Glasser NR, 
            Turnbaugh PJ, Balskus EP
  TITLE     Genetic manipulation of the human gut bacterium Eggerthella lenta 
            reveals a widespread family of transcriptional regulators.
  JOURNAL   Nat Commun. 2022 Dec 9;13(1):7624. doi: 10.1038/s41467-022-33576-3.
  PUBMED    36494336
REFERENCE   2  (bases 1 to 9496)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-12-9"; volume: "13(1):7624. doi"; pages: " 
            10.1038/s41467-022-33576-3"
COMMENT     Sequence Label: pXD70LacZ4-PdegV-LacZ
FEATURES             Location/Qualifiers
     source          1..9496
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      17..88
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     rep_origin      complement(269..857)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(349..368)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1028..1819,1820..1888))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1651..1670
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1889..1993)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2061..2079
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    2236..2255
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that start with ""ATG"""
     CDS             2424..5498
                     /codon_start=1
                     /gene="lacZ"
                     /product="beta-galactosidase"
                     /label=lacZ
                     /translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEART
                     DRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNV
                     TYPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYG
                     QDSRLPSEFDLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQ
                     ISDFHVATRFNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIID
                     ERGGYADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIEN
                     GLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLW
                     YTLCDRYGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWS
                     LGNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVP
                     KWSIKKWLSLPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSL
                     IKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQ
                     TIEVTSEYLFRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQ
                     LWLTVRVVQPNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELG
                     NKRWQFNRQSGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWK
                     AAGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDV
                     EVASDTPHPARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPY
                     VFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDG
                     FHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQK"
     primer_bind     complement(2444..2461)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2444..2460)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2453..2475)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     terminator      5516..5543
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             complement(5666..6460)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
ORIGIN
        1 tggggctttt ctttttcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt
       61 tatctgttgt ttgtcggtga acgctctcta ctagaggagc tcatgccggc ggcacctcgc
      121 taacggattc accactccaa gaattggagc caatcaattc ttgcggagaa ctgtgaatgc
      181 gcaaaccaac ccttggcaga acatatccat cgcgtccgcc atctccagca gccgcacgcg
      241 gcgcatctcg ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
      301 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
      361 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
      421 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
      481 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
      541 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
      601 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
      661 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg
      721 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
      781 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
      841 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
      901 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
      961 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     1021 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     1081 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     1141 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     1201 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     1261 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     1321 tgcgcaacgt tgttgccatt gctgcaggca tcgtggtgtc acgctcgtcg tttggtatgg
     1381 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     1441 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     1501 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     1561 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     1621 cgagttgctc ttgcccggcg tcaacacggg ataataccgc gccacatagc agaactttaa
     1681 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     1741 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     1801 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     1861 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     1921 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     1981 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta
     2041 tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctt caaggcgaaa
     2101 aaaccccgcc gaagcggggt tttttgcgaa atataatgac cctcttgata acccaagagg
     2161 gcatttttta ggaacggtta tttgattatt aatccggcgg caactgaaac gattcggatc
     2221 ctgtattact attctgaatt cgcggccgct tctagatgaa ggagcctttc gcgcgcgtgc
     2281 ggaggaaacg aaacgagcat agcaaggcga ccgtttggag tcaatcttta gaactgcttg
     2341 acatagtacg tttcaaccaa taatctagtt attgatacta gatagtaacc cgggtgatgc
     2401 ccggacgaac gaaagcaggt cgcatgacca tgattacgga ttcactggcc gtcgttttac
     2461 aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc
     2521 ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc
     2581 gcagcctgaa tggcgaatgg cgctttgcct ggtttccggc accagaagcg gtgccggaaa
     2641 gctggctgga gtgcgatctt cctgaggccg atactgtcgt cgtcccctca aactggcaga
     2701 tgcacggtta cgatgcgccc atctacacca acgtgaccta tcccattacg gtcaatccgc
     2761 cgtttgttcc cacggagaat ccgacgggtt gttactcgct cacatttaat gttgatgaaa
     2821 gctggctaca ggaaggccag acgcgaatta tttttgatgg cgttaactcg gcgtttcatc
     2881 tgtggtgcaa cgggcgctgg gtcggttacg gccaggacag tcgtttgccg tctgaatttg
     2941 acctgagcgc atttttacgc gccggagaaa accgcctcgc ggtgatggtg ctgcgctgga
     3001 gtgacggcag ttatctggaa gatcaggata tgtggcggat gagcggcatt ttccgtgacg
     3061 tctcgttgct gcataaaccg actacacaaa tcagcgattt ccatgttgcc actcgcttta
     3121 atgatgattt cagccgcgct gtactggagg ctgaagttca gatgtgcggc gagttgcgtg
     3181 actacctacg ggtaacagtt tctttatggc agggtgaaac gcaggtcgcc agcggcaccg
     3241 cgcctttcgg cggtgaaatt atcgatgagc gtggtggtta tgccgatcgc gtcacactac
     3301 gtctgaacgt cgaaaacccg aaactgtgga gcgccgaaat cccgaatctc tatcgtgcgg
     3361 tggttgaact gcacaccgcc gacggcacgc tgattgaagc agaagcctgc gatgtcggtt
     3421 tccgcgaggt gcggattgaa aatggtctgc tgctgctgaa cggcaagccg ttgctgattc
     3481 gaggcgttaa ccgtcacgag catcatcctc tgcatggtca ggtcatggat gagcagacga
     3541 tggtgcagga tatcctgctg atgaagcaga acaactttaa cgccgtgcgc tgttcgcatt
     3601 atccgaacca tccgctgtgg tacacgctgt gcgaccgcta cggcctgtat gtggtggatg
     3661 aagccaatat tgaaacccac ggcatggtgc caatgaatcg tctgaccgat gatccgcgct
     3721 ggctaccggc gatgagcgaa cgcgtaacgc gaatggtgca gcgcgatcgt aatcacccga
     3781 gtgtgatcat ctggtcgctg gggaatgaat caggccacgg cgctaatcac gacgcgctgt
     3841 atcgctggat caaatctgtc gatccttccc gcccggtgca gtatgaaggc ggcggagccg
     3901 acaccacggc caccgatatt atttgcccga tgtacgcgcg cgtggatgaa gaccagccct
     3961 tcccggctgt gccgaaatgg tccatcaaaa aatggctttc gctacctgga gagacgcgcc
     4021 cgctgatcct ttgcgaatac gcccacgcga tgggtaacag tcttggcggt ttcgctaaat
     4081 actggcaggc gtttcgtcag tatccccgtt tacagggcgg cttcgtctgg gactgggtgg
     4141 atcagtcgct gattaaatat gatgaaaacg gcaacccgtg gtcggcttac ggcggtgatt
     4201 ttggcgatac gccgaacgat cgccagttct gtatgaacgg tctggtcttt gccgaccgca
     4261 cgccgcatcc agcgctgacg gaagcaaaac accagcagca gtttttccag ttccgtttat
     4321 ccgggcaaac catcgaagtg accagcgaat acctgttccg tcatagcgat aacgagctcc
     4381 tgcactggat ggtggcgctg gatggtaagc cgctggcaag cggtgaagtg cctctggatg
     4441 tcgctccaca aggtaaacag ttgattgaac tgcctgaact accgcagccg gagagcgccg
     4501 ggcaactctg gctcacagta cgcgtagtgc aaccgaacgc gaccgcatgg tcagaagccg
     4561 ggcacatcag cgcctggcag cagtggcgtc tggcggaaaa cctcagtgtg acgctccccg
     4621 ccgcgtccca cgccatcccg catctgacca ccagcgaaat ggatttttgc atcgagctgg
     4681 gtaataagcg ttggcaattt aaccgccagt caggctttct ttcacagatg tggattggcg
     4741 ataaaaaaca actgctgacg ccgctgcgcg atcagttcac ccgtgcaccg ctggataacg
     4801 acattggcgt aagtgaagcg acccgcattg accctaacgc ctgggtcgaa cgctggaagg
     4861 cggcgggcca ttaccaggcc gaagcagcgt tgttgcagtg cacggcagat acacttgctg
     4921 atgcggtgct gattacgacc gctcacgcgt ggcagcatca ggggaaaacc ttatttatca
     4981 gccggaaaac ctaccggatt gatggtagtg gtcaaatggc gattaccgtt gatgttgaag
     5041 tggcgagcga tacaccgcat ccggcgcgga ttggcctgaa ctgccagctg gcgcaggtag
     5101 cagagcgggt aaactggctc ggattagggc cgcaagaaaa ctatcccgac cgccttactg
     5161 ccgcctgttt tgaccgctgg gatctgccat tgtcagacat gtataccccg tacgtcttcc
     5221 cgagcgaaaa cggtctgcgc tgcgggacgc gcgaattgaa ttatggccca caccagtggc
     5281 gcggcgactt ccagttcaac atcagccgct acagtcaaca gcaactgatg gaaaccagcc
     5341 atcgccatct gctgcacgcg gaagaaggca catggctgaa tatcgacggt ttccatatgg
     5401 ggattggtgg cgacgactcc tggagcccgt cagtatcggc ggaattccag ctgagcgccg
     5461 gtcgctacca ttaccagttg gtctggtgtc aaaaataaac tagtcgactc acactggctc
     5521 accttcgggt gggcctttct gcgtttatat actagagaga gaatataaaa agccagatta
     5581 ttaatccggc ttttttatta tttaattcct gttataaaaa aaggatcaat tttgaactct
     5641 ctcccaaagt tgatccctta acgatctaaa acaattcatc cagtaaaata taatatttta
     5701 ttttctccca atcaggcttg atccccagta agtcaaaaaa tagctcgaca tactgttctt
     5761 ccccgatatc ctccctgatc gaccggacgc agaaggcaat gtcataccac ttgtccgccc
     5821 tgccgcttct cccaagatca ataaagccac ttactttgcc atctttcaca aagatgttgc
     5881 tgtctcccag gtcgccgtgg gaaaagacaa gttcctcttc gggcttttcc gtctttaaaa
     5941 aatcatacag ctcgcgcgga tctttaaatg gagtgtcttc ttcccagttt tcgcaatcca
     6001 catcggccag atcgttattc agtaagtaat ccaattcggc taagcggctg tctaagctat
     6061 tcgtataggg acaatccgat atgtcgatgg agtgaaagag cctgatgcac tccgcataca
     6121 gctcgataat cttttcaggg ctttgttcat cttcatactc ttccgagcaa aggacgccat
     6181 cggcctcact catgagcaga ttgctccagc catcatgccg ttcaaagtgc aggacctttg
     6241 gaacaggcag ctttccttcc agccatagca tcatgtcctt ttcccgttcc acatcatagg
     6301 tggtcccttt ataccggctg tccgtcattt ttaaatatag gttttcattt tctcccacca
     6361 gcttatatac cttagcagga gacattcctt ccgtatcttt tacgcagcgg tatttttcga
     6421 tcagtttttt caattccggt gatattctca ttttagccat ttattatttc cttcctcttt
     6481 tctacagtat ttaaagatac cccaagaagc taattataac aagacgaact ccaattcact
     6541 gttccttgca ttctaaaacc ttaaatacca gaaaacagct ttttcaaagt tgttttcaaa
     6601 gttggcgtat aacatagtat cgacggagcc gattttgaaa ccacaattat gatagaattt
     6661 acaagctata aggttattgt cctgggtttc aagcattagt ccatgcaagt ttttatgctt
     6721 tgcccattct atagatatat tgataagaag actggaatgc ccgctccgcc gggattattc
     6781 gaaagcgcat cgtccaagcg agaccgtctg cattcggaaa tctcggccgg ttcgcgccga
     6841 ggaagcccat ctggctgcat cccaaatcga cacacaacgc acggaggccc cggcgatcgc
     6901 cggggcctcc gaatcatgcg catcgtggaa gcgaagctcc gcaaaaaacc ccgcttcagc
     6961 ggggtttttt cgcgtcgcac ctccttgtcg tgtattacag gtactactat aacacatcat
     7021 ctagcccgcc cgcgatacgt cctctgcggt gcagcgtggt cgcggttcca cgcatccgcc
     7081 gcccgctgcg catcgcgcag cacgtcatta aggctctgtt gccttggctg ttccctgcgc
     7141 atcagatatt cgagcgggcg cgatattcga cttctaagct cttccacttt gattcgcatt
     7201 cccgcaactc ctgctctgat gcggctcgcg agtccatcgc acttaacaac gatgttatcg
     7261 aggattgcgc cgcgctcagc ttttcccgca gcttcccagc cccgaacctc ggtagcgatg
     7321 ggttgaagct ctccaatgtc tcgcgcaact ccgtccgctc gttgccgaag acactccaat
     7381 cggtcttgct catcttcgat atcgagcttc acgtcctcga gctggctgtc aagctcggca
     7441 aggtcaccct ccttgtcggc aatctcggta acaagctctg ccttacgcgt ttcggcatca
     7501 ttcagtaaag cggcggcttc ttccctagcc gccgcgacga ttcgcgccgc ttcgtccttc
     7561 gcaggttcaa ccacggcctt atcgaccgca cagcgtacct tgtcgatatc cacggacttg
     7621 tccgtgtata cacgctgcgc ccgcgtctcc tcgtcaagct cgattgcagg gcggtagccc
     7681 atgcgcccct ctaggtaatc cccaagctcc ttgtgcatgc tccggtagaa gctgcggggg
     7741 cacatctgct tgtagttgaa ccgtccatct aggatgggcg tgaacggcac gtgcatgtgg
     7801 tcgcgggcgg gcgtgccatc cttcaacacc tcgtccttgt ggacgtaccc gcccatcatg
     7861 ttctcgatgc cgaacttctt cgacaggtac cagtacgtga gacggaagaa cctgtcctca
     7921 tcgcccttgc gcacattgtc gggaagcgtc acaacaacgt cagccataac gaccgcatcc
     7981 ttgcgcgtgg cacgcttccc tgcggccttc tgggcctcgt tcacgcgctc gatgcggctc
     8041 tccaccgtgc cccagttcgg ctcgccatcg cgcggctcca cgcgccccac aacgagcttg
     8101 ccgtccttgt tcgtgtagtg gccgaccacc aagtcgttct tgatgcgctc ggggtcgatg
     8161 ttgtcgcgct ccaggctgct gcggtcgcgg cggtagtggg caagcatgtg cccgaccgat
     8221 ggagccttgt atttcgcgat gtgagccatg gaaacctccg aaccgtacag acttaatcga
     8281 ctaaagtcta gcacgttaga ccttagctac gctaaggacg tggcagggcc agccctgcac
     8341 ccgctcgggc gttccccccg agacccccca gcccttgccc gttcgcggct tcgctcacgg
     8401 ccctcgggcc atcggcgcga gcgccgaacg gagggacttc gcccctccga accacccctc
     8461 aatctacctg cggttcggca cacggcacac ggcactatta ggggggccgt cgtagtggcc
     8521 cccctgtggt catttgtcat ttgtcatttg tcacattatt tttcggacac attatttttc
     8581 ggacgtagtt agcgaaaaat aatgagccgc ccgattctgc aggtctcatc cagtcgagac
     8641 acgggcaagg aaagggtcga tggagcagag cgtccatcga cccttaatca agtctggtct
     8701 attcggttgg ccgtcgcgtc aattccgcaa tccgtgaacg agtcggctta gatctgcgcg
     8761 agcgcagacc acgccgcccc gtagtcacgc attgctgcat tgacccgact actctttggc
     8821 acctgactgt tccacgcact tgcgcttacg ttcggcgttc agatagttcc cgcgacaata
     8881 gcgctcgaga atcgagttgt accccacgat gatttcgcgg tactgctcgg gaaactccct
     8941 cagcgccgca gccgtgaaat cgttgatggt ctcgaacctc tcgcacatga tgaagccgac
     9001 caactcgaag agcatgtcct gcttgtcctt agtcgagagc tgttcgagct gaccaatgtc
     9061 gaagttgttt ccctcgatgc gtgcttcgac cggatacagc tccttgccct tcttgcgaca
     9121 ggaagccgtg tcgtgaatca gatagtcata cgcatgcctg atgttgttga ttggctcggc
     9181 tgacgaacag cacttcttgc cgtccgcgct caatcggttc aagacgttga tgattgcctt
     9241 gcgtgtcgtg ttgccgcccc agacaacaat gacgtgcgca tgcgtcttgc gctgcttgct
     9301 cttctggtca tggtctatgt cgtgaaccgc ataagcgaaa ggcacttgaa gaatgtcgtc
     9361 tatctcgtcc tgccagtcat cgcgcatgtt ctctgggtag cagatgccct cccagagctt
     9421 caccttcgtc tcgccagcgt ttgccgccat gctataatct ccttgtctcg aatgcccctg
     9481 ctgccgccaa gcttgc
//
