LOCUS       sequence-37577-e         942 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 942)
  AUTHORS   Petersen LK, Stowers RS
  TITLE     A Gateway MultiSite Recombination Cloning Toolkit.
  JOURNAL   PLoS One. 2011;6(9):e24531. Epub 2011 Sep 9.
  PUBMED    21931740
REFERENCE   2  (bases 1 to 942)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2011"; volume: "6(9)"; pages: "e24531. Epub 2011 Sep 9"
COMMENT     Sequence Label: pENTR L3-mCherry-HA-L2
FEATURES             Location/Qualifiers
     source          1..942
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    26..125
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(130..156)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             complement(130..156)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(160..864)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     complement(435..454)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     704..722
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     protein_bind    complement(876..896)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
ORIGIN
        1 tcgcggccgc cctgcagctg gatggcaaat aatgatttta ttttgactga tagtgacctg
       61 ttcgttgcaa caaattgata agcaatgctt tcttataatg ccaactttgt acaagaaagc
      121 tgggtattag gcgtagtcgg gcacgtcgta ggggtagccc ttgtacagct cgtccatgcc
      181 gccggtggag tggcggccct cggcgcgttc gtactgttcc acgatggtgt agtcctcgtt
      241 gtgggaggtg atgtccaact tgatgttgac gttgtaggcg ccgggcagct gcacgggctt
      301 cttggccttg taggtggtct tgacctcagc gtcgtagtgg ccgccgtcct tcagcttcag
      361 cctctgcttg atctcgccct tcagggcgcc gtcctcgggg tacatccgct cggaggaggc
      421 ctcccagccc atggtcttct tctgcattac ggggccgtcg gaggggaagt tggtgccgcg
      481 cagcttcacc ttgtagatga actcgccgtc ctgcagggag gagtcctggg tcacggtcac
      541 cacgccgccg tcctcgaagt tcatcacgcg ctcccacttg aagccctcgg ggaaggacag
      601 cttcaagtag tcggggatgt cggcggggtg cttcacgtag gccttggagc cgtacatgaa
      661 ctgaggggac aggatgtccc aggcgaaggg cagggggcca cccttggtca ccttcagctt
      721 ggcggtctgg gtgccctcgt aggggcggcc ctcgccctcg ccctcgatct cgaactcgtg
      781 gccgttcacg gagccctcca tgtgcacctt gaagcgcatg aactccttga tgatggccat
      841 gttatcctcc tcgcccttgc tcacggagcc gccgccaact ttattataca aagttggcat
      901 tataaaaaag cattgctcat caatttgttg caacgaacag gt
//
