LOCUS       sequence-375778-       10872 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  E. coli - Eggerthella lenta shuttle plasmid (KanR), IPTG-inducible 
            promoter-lacZ, engineered from pMAL-c2X.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10872)
  AUTHORS   Dong X, Guthrie BGH, Alexander M, Noecker C, Ramirez L, Glasser NR, 
            Turnbaugh PJ, Balskus EP
  TITLE     Genetic manipulation of the human gut bacterium Eggerthella lenta 
            reveals a widespread family of transcriptional regulators.
  JOURNAL   Nat Commun. 2022 Dec 9;13(1):7624. doi: 10.1038/s41467-022-33576-3.
  PUBMED    36494336
REFERENCE   2  (bases 1 to 10872)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-12-9"; volume: "13(1):7624. doi"; pages: " 
            10.1038/s41467-022-33576-3"
COMMENT     Sequence Label: pXD70LacZ6-LacI-Ptac(LacO)-LacZ
FEATURES             Location/Qualifiers
     source          1..10872
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      44..115
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     rep_origin      complement(296..884)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(376..395)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1055..1846,1847..1915))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1678..1697
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1916..2020)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2088..2106
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    2263..2282
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that start with ""ATG"""
     promoter        2302..2379
                     /gene="lacI (mutant)"
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the 
                     promoter boosts expression of the lacI gene about 10-fold."
     CDS             2380..3462
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     complement(2399..2418)
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        join(3705..3710,3711..3726,3727..3733)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    3741..3757
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3746..3768
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     CDS             3827..6901
                     /codon_start=1
                     /gene="lacZ"
                     /product="beta-galactosidase"
                     /label=lacZ
                     /translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEART
                     DRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNV
                     TYPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYG
                     QDSRLPSEFDLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQ
                     ISDFHVATRFNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIID
                     ERGGYADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIEN
                     GLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLW
                     YTLCDRYGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWS
                     LGNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVP
                     KWSIKKWLSLPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSL
                     IKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQ
                     TIEVTSEYLFRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQ
                     LWLTVRVVQPNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELG
                     NKRWQFNRQSGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWK
                     AAGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDV
                     EVASDTPHPARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPY
                     VFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDG
                     FHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQK"
     primer_bind     complement(3847..3864)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3847..3863)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3856..3878)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     terminator      6919..6946
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             complement(7069..7863)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
ORIGIN
        1 gaatgcccct gctgccgcca agcttgctgg ggcttttctt tttcaaataa aacgaaaggc
       61 tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctctacta
      121 gaggagctca tgccggcggc acctcgctaa cggattcacc actccaagaa ttggagccaa
      181 tcaattcttg cggagaactg tgaatgcgca aaccaaccct tggcagaaca tatccatcgc
      241 gtccgccatc tccagcagcc gcacgcggcg catctcgggc cgcgttgctg gcgtttttcc
      301 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
      361 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
      421 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
      481 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
      541 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
      601 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
      661 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
      721 acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
      781 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
      841 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
      901 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
      961 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     1021 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     1081 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     1141 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
     1201 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     1261 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     1321 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct gcaggcatcg
     1381 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     1441 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
     1501 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
     1561 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
     1621 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca acacgggata
     1681 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
     1741 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
     1801 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
     1861 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
     1921 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     1981 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
     2041 cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca
     2101 cgaggccctt tcgtcttcaa ggcgaaaaaa ccccgccgaa gcggggtttt ttgcgaaata
     2161 taatgaccct cttgataacc caagagggca ttttttagga acggttattt gattattaat
     2221 ccggcggcaa ctgaaacgat tcggatcctg tattactatt ctgaattcgc ggccgcttct
     2281 agatacgctg cccgaaattc cgacaccatc gaatggtgca aaacctttcg cggtatggca
     2341 tgatagcgcc cggaagagag tcaattcagg gtggtgaatg tgaaaccagt aacgttatac
     2401 gatgtcgcag agtatgccgg tgtctcttat cagaccgttt cccgcgtggt gaaccaggcc
     2461 agccacgttt ctgcgaaaac gcgggaaaaa gtggaagcgg cgatggcgga gctgaattac
     2521 attcccaacc gcgtggcaca acaactggcg ggcaaacagt cgttgctgat tggcgttgcc
     2581 acctccagtc tggccctgca cgcgccgtcg caaattgtcg cggcgattaa atctcgcgcc
     2641 gatcaactgg gtgccagcgt ggtggtgtcg atggtagaac gaagcggcgt cgaagcctgt
     2701 aaagcggcgg tgcacaatct tctcgcgcaa cgcgtcagtg ggctgatcat taactatccg
     2761 ctggatgacc aggatgccat tgctgtggaa gctgcctgca ctaatgttcc ggcgttattt
     2821 cttgatgtct ctgaccagac acccatcaac agtattattt tctcccatga agacggtacg
     2881 cgactgggcg tggagcatct ggtcgcattg ggtcaccagc aaatcgcgct gttagcgggc
     2941 ccattaagtt ctgtctcggc gcgtctgcgt ctggctggct ggcataaata tctcactcgc
     3001 aatcaaattc agccgatagc ggaacgggaa ggcgactgga gtgccatgtc cggttttcaa
     3061 caaaccatgc aaatgctgaa tgagggcatc gttcccactg cgatgctggt tgccaacgat
     3121 cagatggcgc tgggcgcaat gcgcgccatt accgagtccg ggctgcgcgt tggtgcggat
     3181 atctcggtag tgggatacga cgataccgaa gacagctcat gttatatccc gccgttaacc
     3241 accatcaaac aggattttcg cctgctgggg caaaccagcg tggaccgctt gctgcaactc
     3301 tctcagggcc aggcggtgaa gggcaatcag ctgttgcccg tctcactggt gaaaagaaaa
     3361 accaccctgg cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg
     3421 cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt
     3481 aagttagctc actcattagg cacaattctc atgtttgaca gcttatcatc gactgcacgg
     3541 tgcaccaatg cttctggcgt caggcagcca tcggaagctg tggtatggct gtgcaggtcg
     3601 taaatcactg cataattcgt gtcgctcaag gcgcactccc gttctggata atgttttttg
     3661 cgccgacatc ataacggttc tggcaaatat tctgaaatga gctgttgaca attaatcatc
     3721 ggctcgtata atgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagccagtc
     3781 cgtttaggtg ttttcacgag cacttcacca acaaggacca tagcatatga ccatgattac
     3841 ggattcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact
     3901 taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac
     3961 cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tggcgctttg cctggtttcc
     4021 ggcaccagaa gcggtgccgg aaagctggct ggagtgcgat cttcctgagg ccgatactgt
     4081 cgtcgtcccc tcaaactggc agatgcacgg ttacgatgcg cccatctaca ccaacgtgac
     4141 ctatcccatt acggtcaatc cgccgtttgt tcccacggag aatccgacgg gttgttactc
     4201 gctcacattt aatgttgatg aaagctggct acaggaaggc cagacgcgaa ttatttttga
     4261 tggcgttaac tcggcgtttc atctgtggtg caacgggcgc tgggtcggtt acggccagga
     4321 cagtcgtttg ccgtctgaat ttgacctgag cgcattttta cgcgccggag aaaaccgcct
     4381 cgcggtgatg gtgctgcgct ggagtgacgg cagttatctg gaagatcagg atatgtggcg
     4441 gatgagcggc attttccgtg acgtctcgtt gctgcataaa ccgactacac aaatcagcga
     4501 tttccatgtt gccactcgct ttaatgatga tttcagccgc gctgtactgg aggctgaagt
     4561 tcagatgtgc ggcgagttgc gtgactacct acgggtaaca gtttctttat ggcagggtga
     4621 aacgcaggtc gccagcggca ccgcgccttt cggcggtgaa attatcgatg agcgtggtgg
     4681 ttatgccgat cgcgtcacac tacgtctgaa cgtcgaaaac ccgaaactgt ggagcgccga
     4741 aatcccgaat ctctatcgtg cggtggttga actgcacacc gccgacggca cgctgattga
     4801 agcagaagcc tgcgatgtcg gtttccgcga ggtgcggatt gaaaatggtc tgctgctgct
     4861 gaacggcaag ccgttgctga ttcgaggcgt taaccgtcac gagcatcatc ctctgcatgg
     4921 tcaggtcatg gatgagcaga cgatggtgca ggatatcctg ctgatgaagc agaacaactt
     4981 taacgccgtg cgctgttcgc attatccgaa ccatccgctg tggtacacgc tgtgcgaccg
     5041 ctacggcctg tatgtggtgg atgaagccaa tattgaaacc cacggcatgg tgccaatgaa
     5101 tcgtctgacc gatgatccgc gctggctacc ggcgatgagc gaacgcgtaa cgcgaatggt
     5161 gcagcgcgat cgtaatcacc cgagtgtgat catctggtcg ctggggaatg aatcaggcca
     5221 cggcgctaat cacgacgcgc tgtatcgctg gatcaaatct gtcgatcctt cccgcccggt
     5281 gcagtatgaa ggcggcggag ccgacaccac ggccaccgat attatttgcc cgatgtacgc
     5341 gcgcgtggat gaagaccagc ccttcccggc tgtgccgaaa tggtccatca aaaaatggct
     5401 ttcgctacct ggagagacgc gcccgctgat cctttgcgaa tacgcccacg cgatgggtaa
     5461 cagtcttggc ggtttcgcta aatactggca ggcgtttcgt cagtatcccc gtttacaggg
     5521 cggcttcgtc tgggactggg tggatcagtc gctgattaaa tatgatgaaa acggcaaccc
     5581 gtggtcggct tacggcggtg attttggcga tacgccgaac gatcgccagt tctgtatgaa
     5641 cggtctggtc tttgccgacc gcacgccgca tccagcgctg acggaagcaa aacaccagca
     5701 gcagtttttc cagttccgtt tatccgggca aaccatcgaa gtgaccagcg aatacctgtt
     5761 ccgtcatagc gataacgagc tcctgcactg gatggtggcg ctggatggta agccgctggc
     5821 aagcggtgaa gtgcctctgg atgtcgctcc acaaggtaaa cagttgattg aactgcctga
     5881 actaccgcag ccggagagcg ccgggcaact ctggctcaca gtacgcgtag tgcaaccgaa
     5941 cgcgaccgca tggtcagaag ccgggcacat cagcgcctgg cagcagtggc gtctggcgga
     6001 aaacctcagt gtgacgctcc ccgccgcgtc ccacgccatc ccgcatctga ccaccagcga
     6061 aatggatttt tgcatcgagc tgggtaataa gcgttggcaa tttaaccgcc agtcaggctt
     6121 tctttcacag atgtggattg gcgataaaaa acaactgctg acgccgctgc gcgatcagtt
     6181 cacccgtgca ccgctggata acgacattgg cgtaagtgaa gcgacccgca ttgaccctaa
     6241 cgcctgggtc gaacgctgga aggcggcggg ccattaccag gccgaagcag cgttgttgca
     6301 gtgcacggca gatacacttg ctgatgcggt gctgattacg accgctcacg cgtggcagca
     6361 tcaggggaaa accttattta tcagccggaa aacctaccgg attgatggta gtggtcaaat
     6421 ggcgattacc gttgatgttg aagtggcgag cgatacaccg catccggcgc ggattggcct
     6481 gaactgccag ctggcgcagg tagcagagcg ggtaaactgg ctcggattag ggccgcaaga
     6541 aaactatccc gaccgcctta ctgccgcctg ttttgaccgc tgggatctgc cattgtcaga
     6601 catgtatacc ccgtacgtct tcccgagcga aaacggtctg cgctgcggga cgcgcgaatt
     6661 gaattatggc ccacaccagt ggcgcggcga cttccagttc aacatcagcc gctacagtca
     6721 acagcaactg atggaaacca gccatcgcca tctgctgcac gcggaagaag gcacatggct
     6781 gaatatcgac ggtttccata tggggattgg tggcgacgac tcctggagcc cgtcagtatc
     6841 ggcggaattc cagctgagcg ccggtcgcta ccattaccag ttggtctggt gtcaaaaata
     6901 aactagtcga ctcacactgg ctcaccttcg ggtgggcctt tctgcgttta tatactagag
     6961 agagaatata aaaagccaga ttattaatcc ggctttttta ttatttaatt cctgttataa
     7021 aaaaaggatc aattttgaac tctctcccaa agttgatccc ttaacgatct aaaacaattc
     7081 atccagtaaa atataatatt ttattttctc ccaatcaggc ttgatcccca gtaagtcaaa
     7141 aaatagctcg acatactgtt cttccccgat atcctccctg atcgaccgga cgcagaaggc
     7201 aatgtcatac cacttgtccg ccctgccgct tctcccaaga tcaataaagc cacttacttt
     7261 gccatctttc acaaagatgt tgctgtctcc caggtcgccg tgggaaaaga caagttcctc
     7321 ttcgggcttt tccgtcttta aaaaatcata cagctcgcgc ggatctttaa atggagtgtc
     7381 ttcttcccag ttttcgcaat ccacatcggc cagatcgtta ttcagtaagt aatccaattc
     7441 ggctaagcgg ctgtctaagc tattcgtata gggacaatcc gatatgtcga tggagtgaaa
     7501 gagcctgatg cactccgcat acagctcgat aatcttttca gggctttgtt catcttcata
     7561 ctcttccgag caaaggacgc catcggcctc actcatgagc agattgctcc agccatcatg
     7621 ccgttcaaag tgcaggacct ttggaacagg cagctttcct tccagccata gcatcatgtc
     7681 cttttcccgt tccacatcat aggtggtccc tttataccgg ctgtccgtca tttttaaata
     7741 taggttttca ttttctccca ccagcttata taccttagca ggagacattc cttccgtatc
     7801 ttttacgcag cggtattttt cgatcagttt tttcaattcc ggtgatattc tcattttagc
     7861 catttattat ttccttcctc ttttctacag tatttaaaga taccccaaga agctaattat
     7921 aacaagacga actccaattc actgttcctt gcattctaaa accttaaata ccagaaaaca
     7981 gctttttcaa agttgttttc aaagttggcg tataacatag tatcgacgga gccgattttg
     8041 aaaccacaat tatgatagaa tttacaagct ataaggttat tgtcctgggt ttcaagcatt
     8101 agtccatgca agtttttatg ctttgcccat tctatagata tattgataag aagactggaa
     8161 tgcccgctcc gccgggatta ttcgaaagcg catcgtccaa gcgagaccgt ctgcattcgg
     8221 aaatctcggc cggttcgcgc cgaggaagcc catctggctg catcccaaat cgacacacaa
     8281 cgcacggagg ccccggcgat cgccggggcc tccgaatcat gcgcatcgtg gaagcgaagc
     8341 tccgcaaaaa accccgcttc agcggggttt tttcgcgtcg cacctccttg tcgtgtatta
     8401 caggtactac tataacacat catctagccc gcccgcgata cgtcctctgc ggtgcagcgt
     8461 ggtcgcggtt ccacgcatcc gccgcccgct gcgcatcgcg cagcacgtca ttaaggctct
     8521 gttgccttgg ctgttccctg cgcatcagat attcgagcgg gcgcgatatt cgacttctaa
     8581 gctcttccac tttgattcgc attcccgcaa ctcctgctct gatgcggctc gcgagtccat
     8641 cgcacttaac aacgatgtta tcgaggattg cgccgcgctc agcttttccc gcagcttccc
     8701 agccccgaac ctcggtagcg atgggttgaa gctctccaat gtctcgcgca actccgtccg
     8761 ctcgttgccg aagacactcc aatcggtctt gctcatcttc gatatcgagc ttcacgtcct
     8821 cgagctggct gtcaagctcg gcaaggtcac cctccttgtc ggcaatctcg gtaacaagct
     8881 ctgccttacg cgtttcggca tcattcagta aagcggcggc ttcttcccta gccgccgcga
     8941 cgattcgcgc cgcttcgtcc ttcgcaggtt caaccacggc cttatcgacc gcacagcgta
     9001 ccttgtcgat atccacggac ttgtccgtgt atacacgctg cgcccgcgtc tcctcgtcaa
     9061 gctcgattgc agggcggtag cccatgcgcc cctctaggta atccccaagc tccttgtgca
     9121 tgctccggta gaagctgcgg gggcacatct gcttgtagtt gaaccgtcca tctaggatgg
     9181 gcgtgaacgg cacgtgcatg tggtcgcggg cgggcgtgcc atccttcaac acctcgtcct
     9241 tgtggacgta cccgcccatc atgttctcga tgccgaactt cttcgacagg taccagtacg
     9301 tgagacggaa gaacctgtcc tcatcgccct tgcgcacatt gtcgggaagc gtcacaacaa
     9361 cgtcagccat aacgaccgca tccttgcgcg tggcacgctt ccctgcggcc ttctgggcct
     9421 cgttcacgcg ctcgatgcgg ctctccaccg tgccccagtt cggctcgcca tcgcgcggct
     9481 ccacgcgccc cacaacgagc ttgccgtcct tgttcgtgta gtggccgacc accaagtcgt
     9541 tcttgatgcg ctcggggtcg atgttgtcgc gctccaggct gctgcggtcg cggcggtagt
     9601 gggcaagcat gtgcccgacc gatggagcct tgtatttcgc gatgtgagcc atggaaacct
     9661 ccgaaccgta cagacttaat cgactaaagt ctagcacgtt agaccttagc tacgctaagg
     9721 acgtggcagg gccagccctg cacccgctcg ggcgttcccc ccgagacccc ccagcccttg
     9781 cccgttcgcg gcttcgctca cggccctcgg gccatcggcg cgagcgccga acggagggac
     9841 ttcgcccctc cgaaccaccc ctcaatctac ctgcggttcg gcacacggca cacggcacta
     9901 ttaggggggc cgtcgtagtg gcccccctgt ggtcatttgt catttgtcat ttgtcacatt
     9961 atttttcgga cacattattt ttcggacgta gttagcgaaa aataatgagc cgcccgattc
    10021 tgcaggtctc atccagtcga gacacgggca aggaaagggt cgatggagca gagcgtccat
    10081 cgacccttaa tcaagtctgg tctattcggt tggccgtcgc gtcaattccg caatccgtga
    10141 acgagtcggc ttagatctgc gcgagcgcag accacgccgc cccgtagtca cgcattgctg
    10201 cattgacccg actactcttt ggcacctgac tgttccacgc acttgcgctt acgttcggcg
    10261 ttcagatagt tcccgcgaca atagcgctcg agaatcgagt tgtaccccac gatgatttcg
    10321 cggtactgct cgggaaactc cctcagcgcc gcagccgtga aatcgttgat ggtctcgaac
    10381 ctctcgcaca tgatgaagcc gaccaactcg aagagcatgt cctgcttgtc cttagtcgag
    10441 agctgttcga gctgaccaat gtcgaagttg tttccctcga tgcgtgcttc gaccggatac
    10501 agctccttgc ccttcttgcg acaggaagcc gtgtcgtgaa tcagatagtc atacgcatgc
    10561 ctgatgttgt tgattggctc ggctgacgaa cagcacttct tgccgtccgc gctcaatcgg
    10621 ttcaagacgt tgatgattgc cttgcgtgtc gtgttgccgc cccagacaac aatgacgtgc
    10681 gcatgcgtct tgcgctgctt gctcttctgg tcatggtcta tgtcgtgaac cgcataagcg
    10741 aaaggcactt gaagaatgtc gtctatctcg tcctgccagt catcgcgcat gttctctggg
    10801 tagcagatgc cctcccagag cttcaccttc gtctcgccag cgtttgccgc catgctataa
    10861 tctccttgtc tc
//
