LOCUS       sequence-375780-       11773 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  E. coli - Eggerthella lenta shuttle plasmid (KanR), Pdadh dopamine 
            dehydroxylase promoter-lacZ with transcriptional regulators 
            DadR/DadS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11773)
  AUTHORS   Dong X, Guthrie BGH, Alexander M, Noecker C, Ramirez L, Glasser NR, 
            Turnbaugh PJ, Balskus EP
  TITLE     Genetic manipulation of the human gut bacterium Eggerthella lenta 
            reveals a widespread family of transcriptional regulators.
  JOURNAL   Nat Commun. 2022 Dec 9;13(1):7624. doi: 10.1038/s41467-022-33576-3.
  PUBMED    36494336
REFERENCE   2  (bases 1 to 11773)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-12-9"; volume: "13(1):7624. doi"; pages: " 
            10.1038/s41467-022-33576-3"
COMMENT     Sequence Label: pXD70LacZ7-DadR/DadS-Pdadh-LacZ
FEATURES             Location/Qualifiers
     source          1..11773
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      34..105
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     rep_origin      complement(286..874)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(366..385)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1045..1836,1837..1905))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1668..1687
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1906..2010)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2078..2096
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    2253..2272
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that start with ""ATG"""
     CDS             4718..7792
                     /codon_start=1
                     /gene="lacZ"
                     /product="beta-galactosidase"
                     /label=lacZ
                     /translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEART
                     DRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNV
                     TYPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYG
                     QDSRLPSEFDLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQ
                     ISDFHVATRFNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIID
                     ERGGYADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIEN
                     GLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLW
                     YTLCDRYGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWS
                     LGNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVP
                     KWSIKKWLSLPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSL
                     IKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQ
                     TIEVTSEYLFRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQ
                     LWLTVRVVQPNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELG
                     NKRWQFNRQSGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWK
                     AAGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDV
                     EVASDTPHPARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPY
                     VFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDG
                     FHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQK"
     primer_bind     complement(4738..4755)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4738..4754)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4747..4769)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     terminator      7810..7837
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             complement(7960..8754)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
ORIGIN
        1 gctgccgcca agcttgctgg ggcttttctt tttcaaataa aacgaaaggc tcagtcgaaa
       61 gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctctacta gaggagctca
      121 tgccggcggc acctcgctaa cggattcacc actccaagaa ttggagccaa tcaattcttg
      181 cggagaactg tgaatgcgca aaccaaccct tggcagaaca tatccatcgc gtccgccatc
      241 tccagcagcc gcacgcggcg catctcgggc cgcgttgctg gcgtttttcc ataggctccg
      301 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
      361 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
      421 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
      481 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
      541 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
      601 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
      661 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
      721 tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
      781 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
      841 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
      901 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
      961 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     1021 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     1081 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     1141 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
     1201 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     1261 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     1321 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct gcaggcatcg tggtgtcacg
     1381 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     1441 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     1501 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     1561 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     1621 atagtgtatg cggcgaccga gttgctcttg cccggcgtca acacgggata ataccgcgcc
     1681 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     1741 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     1801 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
     1861 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
     1921 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
     1981 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt
     2041 ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt
     2101 tcgtcttcaa ggcgaaaaaa ccccgccgaa gcggggtttt ttgcgaaata taatgaccct
     2161 cttgataacc caagagggca ttttttagga acggttattt gattattaat ccggcggcaa
     2221 ctgaaacgat tcggatcctg tattactatt ctgaattcgc ggccgcttct agattcattc
     2281 cgaaacggaa tggatgaaat cgagcgcctc ctgcttgtcg cccacgccca gcttgcggta
     2341 cacgctgccc agcacgtcgg ccacctcgtc gcgcgtcagc gccagctcgc cctggatcgc
     2401 ctcgaagccg cgctcctcca cgtacaggat gagcgcgccc agctcgcgcc ggtcgagccc
     2461 gcagcgctcg gccgcgagcc ggcacttgca gcgaagcgcc gtgtcgcacg cggccgcagg
     2521 cgagccgtcg acggcacccc cgcccgccgc tgcgcccgcc gtcgcgcccg caacccccgc
     2581 gcccgccgaa ccctgcccgt cgcgcgccgc gcgcaccagc agctcggtgg cgcgcagcag
     2641 cttctccatc tgccgcgcct gggcgtacag cacgccgccc gcctcggtca ggctcacgcc
     2701 gctgcggtcg cgcacgaaca ggtcggcccc gaattcgcgc tccaacaagg ccacgtgctt
     2761 gctcagcgtc gactgcgtca gatgcagctc gcgcgcggcg gccgtgaagc tgccgcaatc
     2821 ggccaccttc gcgaactcgt gcagatacgc cgtctccatc gcctcagacc gcctcgtcct
     2881 gcgcggccgt ctcgtgggcc aggtcgatga gctcctggcg attgtccacg cccgtcttct
     2941 ggtagatgtg gcggatatgc gtgctcaccg tgcccgccga gatgaacagc gtctcctgga
     3001 tgcgcgagat ggtgcggccc tccagcagca gcggaagcac gtcggtctcg cgcggcgaca
     3061 gggcgaagcg ctccgcgatc agcgcgcacg gatccaccgg gtcggccggg tcgtcggcgg
     3121 cctcttcggg caccgtgccc atcaggctga cctcgccgat gccgattttg atgaggtcgg
     3181 tctccgtgaa caggaacaaa tgcgcgatga acagcagcga cacggccacc agcgtgacca
     3241 gcgtgaggtc gaacatgctg accccggtcg cctccagcga atgggcgaac agctggcctg
     3301 cggtctcgcc gccgtacagc gccacgaacc ccaggccgat cgtccgcgcc gggctcacgc
     3361 ggaagctgga cgccacgtcg atgcacacca cgcacagcac cagcacgaag cagtggtagc
     3421 cgccgaagat aagcgcgctc gaatacgtgt tgttgtcggc catgaacggc gtggacaagc
     3481 atcccaagca cagcatcagc atggagacgc ggtacgagaa gaacacgctg tcgcgctgca
     3541 ggaacacggc tatcaggcag atggccaacg ctccgatgaa gcccgccaga aacaggtacg
     3601 tgccgaagat gtcctgcacc tcgtaggttt tgaaccccga caccacgtcg aagaacccca
     3661 tcaacatgcc gatgagcacc gcgcccagaa gcgccttcgc gaacagggtc gccgtatggc
     3721 gcgacaaagg ctgctcgggc tcgcgcggca gctgcggcgt atcgggcgcg ctgcgcctga
     3781 gagctgccgc cgaaagcacg ggcatgacaa cgatgagcac gatgacgggt atcgccggcc
     3841 ctacgatcag caagaacccc acgacgaacg cgatgcacag cgacgaggca agctccacaa
     3901 gcgccaagcg gcctcccagc cccgactcga gctcgatcca gcacaagaac agcacggccg
     3961 agcccgtgcc ggtcatgacc gcgctcaggc ccagcaccag catgccgacg ggcgtggtag
     4021 gatcggagaa cggcgtgagc acgctgcccg cgctcatcat gatggccgat cccagcacga
     4081 tggagcggtt gccgctcacc cgctcgcgcc gcacgatgac ggagacgaac agcatggtca
     4141 ccaccatggc caccagcgat atgatcaggc tgacgtacag cgagccgcgc acgtccgacg
     4201 agaagccgta cggcacgatg ctgtagaaca tcatgagcat ccaaagccat tgcaaggccg
     4261 cgcccgcgat gcgcttgagg gaaaggctcg tccccaacgc ggtatcccat gcactccgaa
     4321 gcttttccat gccccacccc ctgtgagccc gtacataacg tcgctcgcgc cgacccctct
     4381 gtcgacgggt tcgattatac ggaaaagatg agcggccctc tttgaggaaa ctattctagt
     4441 ttcagaataa tttatgacct acggttaaag tttccccagt tcaaagcact atcgaatttt
     4501 tcatgatcac aagatcataa gaatttatga tgcggtcgaa cctgctttcc aacctttgga
     4561 cgatgctctg caccccctcg catccgtatg gtcggcccaa gcgacacggg gactttgcac
     4621 cggttcggag cccgaggctt cacccggcag cccccctccc cgatcgcacg aacgaagacg
     4681 agcaagacga gaacgaggag aaagaaggag gaggagtatg accatgatta cggattcact
     4741 ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct
     4801 tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc
     4861 ttcccaacag ttgcgcagcc tgaatggcga atggcgcttt gcctggtttc cggcaccaga
     4921 agcggtgccg gaaagctggc tggagtgcga tcttcctgag gccgatactg tcgtcgtccc
     4981 ctcaaactgg cagatgcacg gttacgatgc gcccatctac accaacgtga cctatcccat
     5041 tacggtcaat ccgccgtttg ttcccacgga gaatccgacg ggttgttact cgctcacatt
     5101 taatgttgat gaaagctggc tacaggaagg ccagacgcga attatttttg atggcgttaa
     5161 ctcggcgttt catctgtggt gcaacgggcg ctgggtcggt tacggccagg acagtcgttt
     5221 gccgtctgaa tttgacctga gcgcattttt acgcgccgga gaaaaccgcc tcgcggtgat
     5281 ggtgctgcgc tggagtgacg gcagttatct ggaagatcag gatatgtggc ggatgagcgg
     5341 cattttccgt gacgtctcgt tgctgcataa accgactaca caaatcagcg atttccatgt
     5401 tgccactcgc tttaatgatg atttcagccg cgctgtactg gaggctgaag ttcagatgtg
     5461 cggcgagttg cgtgactacc tacgggtaac agtttcttta tggcagggtg aaacgcaggt
     5521 cgccagcggc accgcgcctt tcggcggtga aattatcgat gagcgtggtg gttatgccga
     5581 tcgcgtcaca ctacgtctga acgtcgaaaa cccgaaactg tggagcgccg aaatcccgaa
     5641 tctctatcgt gcggtggttg aactgcacac cgccgacggc acgctgattg aagcagaagc
     5701 ctgcgatgtc ggtttccgcg aggtgcggat tgaaaatggt ctgctgctgc tgaacggcaa
     5761 gccgttgctg attcgaggcg ttaaccgtca cgagcatcat cctctgcatg gtcaggtcat
     5821 ggatgagcag acgatggtgc aggatatcct gctgatgaag cagaacaact ttaacgccgt
     5881 gcgctgttcg cattatccga accatccgct gtggtacacg ctgtgcgacc gctacggcct
     5941 gtatgtggtg gatgaagcca atattgaaac ccacggcatg gtgccaatga atcgtctgac
     6001 cgatgatccg cgctggctac cggcgatgag cgaacgcgta acgcgaatgg tgcagcgcga
     6061 tcgtaatcac ccgagtgtga tcatctggtc gctggggaat gaatcaggcc acggcgctaa
     6121 tcacgacgcg ctgtatcgct ggatcaaatc tgtcgatcct tcccgcccgg tgcagtatga
     6181 aggcggcgga gccgacacca cggccaccga tattatttgc ccgatgtacg cgcgcgtgga
     6241 tgaagaccag cccttcccgg ctgtgccgaa atggtccatc aaaaaatggc tttcgctacc
     6301 tggagagacg cgcccgctga tcctttgcga atacgcccac gcgatgggta acagtcttgg
     6361 cggtttcgct aaatactggc aggcgtttcg tcagtatccc cgtttacagg gcggcttcgt
     6421 ctgggactgg gtggatcagt cgctgattaa atatgatgaa aacggcaacc cgtggtcggc
     6481 ttacggcggt gattttggcg atacgccgaa cgatcgccag ttctgtatga acggtctggt
     6541 ctttgccgac cgcacgccgc atccagcgct gacggaagca aaacaccagc agcagttttt
     6601 ccagttccgt ttatccgggc aaaccatcga agtgaccagc gaatacctgt tccgtcatag
     6661 cgataacgag ctcctgcact ggatggtggc gctggatggt aagccgctgg caagcggtga
     6721 agtgcctctg gatgtcgctc cacaaggtaa acagttgatt gaactgcctg aactaccgca
     6781 gccggagagc gccgggcaac tctggctcac agtacgcgta gtgcaaccga acgcgaccgc
     6841 atggtcagaa gccgggcaca tcagcgcctg gcagcagtgg cgtctggcgg aaaacctcag
     6901 tgtgacgctc cccgccgcgt cccacgccat cccgcatctg accaccagcg aaatggattt
     6961 ttgcatcgag ctgggtaata agcgttggca atttaaccgc cagtcaggct ttctttcaca
     7021 gatgtggatt ggcgataaaa aacaactgct gacgccgctg cgcgatcagt tcacccgtgc
     7081 accgctggat aacgacattg gcgtaagtga agcgacccgc attgacccta acgcctgggt
     7141 cgaacgctgg aaggcggcgg gccattacca ggccgaagca gcgttgttgc agtgcacggc
     7201 agatacactt gctgatgcgg tgctgattac gaccgctcac gcgtggcagc atcaggggaa
     7261 aaccttattt atcagccgga aaacctaccg gattgatggt agtggtcaaa tggcgattac
     7321 cgttgatgtt gaagtggcga gcgatacacc gcatccggcg cggattggcc tgaactgcca
     7381 gctggcgcag gtagcagagc gggtaaactg gctcggatta gggccgcaag aaaactatcc
     7441 cgaccgcctt actgccgcct gttttgaccg ctgggatctg ccattgtcag acatgtatac
     7501 cccgtacgtc ttcccgagcg aaaacggtct gcgctgcggg acgcgcgaat tgaattatgg
     7561 cccacaccag tggcgcggcg acttccagtt caacatcagc cgctacagtc aacagcaact
     7621 gatggaaacc agccatcgcc atctgctgca cgcggaagaa ggcacatggc tgaatatcga
     7681 cggtttccat atggggattg gtggcgacga ctcctggagc ccgtcagtat cggcggaatt
     7741 ccagctgagc gccggtcgct accattacca gttggtctgg tgtcaaaaat aaactagtcg
     7801 actcacactg gctcaccttc gggtgggcct ttctgcgttt atatactaga gagagaatat
     7861 aaaaagccag attattaatc cggctttttt attatttaat tcctgttata aaaaaaggat
     7921 caattttgaa ctctctccca aagttgatcc cttaacgatc taaaacaatt catccagtaa
     7981 aatataatat tttattttct cccaatcagg cttgatcccc agtaagtcaa aaaatagctc
     8041 gacatactgt tcttccccga tatcctccct gatcgaccgg acgcagaagg caatgtcata
     8101 ccacttgtcc gccctgccgc ttctcccaag atcaataaag ccacttactt tgccatcttt
     8161 cacaaagatg ttgctgtctc ccaggtcgcc gtgggaaaag acaagttcct cttcgggctt
     8221 ttccgtcttt aaaaaatcat acagctcgcg cggatcttta aatggagtgt cttcttccca
     8281 gttttcgcaa tccacatcgg ccagatcgtt attcagtaag taatccaatt cggctaagcg
     8341 gctgtctaag ctattcgtat agggacaatc cgatatgtcg atggagtgaa agagcctgat
     8401 gcactccgca tacagctcga taatcttttc agggctttgt tcatcttcat actcttccga
     8461 gcaaaggacg ccatcggcct cactcatgag cagattgctc cagccatcat gccgttcaaa
     8521 gtgcaggacc tttggaacag gcagctttcc ttccagccat agcatcatgt ccttttcccg
     8581 ttccacatca taggtggtcc ctttataccg gctgtccgtc atttttaaat ataggttttc
     8641 attttctccc accagcttat ataccttagc aggagacatt ccttccgtat cttttacgca
     8701 gcggtatttt tcgatcagtt ttttcaattc cggtgatatt ctcattttag ccatttatta
     8761 tttccttcct cttttctaca gtatttaaag ataccccaag aagctaatta taacaagacg
     8821 aactccaatt cactgttcct tgcattctaa aaccttaaat accagaaaac agctttttca
     8881 aagttgtttt caaagttggc gtataacata gtatcgacgg agccgatttt gaaaccacaa
     8941 ttatgataga atttacaagc tataaggtta ttgtcctggg tttcaagcat tagtccatgc
     9001 aagtttttat gctttgccca ttctatagat atattgataa gaagactgga atgcccgctc
     9061 cgccgggatt attcgaaagc gcatcgtcca agcgagaccg tctgcattcg gaaatctcgg
     9121 ccggttcgcg ccgaggaagc ccatctggct gcatcccaaa tcgacacaca acgcacggag
     9181 gccccggcga tcgccggggc ctccgaatca tgcgcatcgt ggaagcgaag ctccgcaaaa
     9241 aaccccgctt cagcggggtt ttttcgcgtc gcacctcctt gtcgtgtatt acaggtacta
     9301 ctataacaca tcatctagcc cgcccgcgat acgtcctctg cggtgcagcg tggtcgcggt
     9361 tccacgcatc cgccgcccgc tgcgcatcgc gcagcacgtc attaaggctc tgttgccttg
     9421 gctgttccct gcgcatcaga tattcgagcg ggcgcgatat tcgacttcta agctcttcca
     9481 ctttgattcg cattcccgca actcctgctc tgatgcggct cgcgagtcca tcgcacttaa
     9541 caacgatgtt atcgaggatt gcgccgcgct cagcttttcc cgcagcttcc cagccccgaa
     9601 cctcggtagc gatgggttga agctctccaa tgtctcgcgc aactccgtcc gctcgttgcc
     9661 gaagacactc caatcggtct tgctcatctt cgatatcgag cttcacgtcc tcgagctggc
     9721 tgtcaagctc ggcaaggtca ccctccttgt cggcaatctc ggtaacaagc tctgccttac
     9781 gcgtttcggc atcattcagt aaagcggcgg cttcttccct agccgccgcg acgattcgcg
     9841 ccgcttcgtc cttcgcaggt tcaaccacgg ccttatcgac cgcacagcgt accttgtcga
     9901 tatccacgga cttgtccgtg tatacacgct gcgcccgcgt ctcctcgtca agctcgattg
     9961 cagggcggta gcccatgcgc ccctctaggt aatccccaag ctccttgtgc atgctccggt
    10021 agaagctgcg ggggcacatc tgcttgtagt tgaaccgtcc atctaggatg ggcgtgaacg
    10081 gcacgtgcat gtggtcgcgg gcgggcgtgc catccttcaa cacctcgtcc ttgtggacgt
    10141 acccgcccat catgttctcg atgccgaact tcttcgacag gtaccagtac gtgagacgga
    10201 agaacctgtc ctcatcgccc ttgcgcacat tgtcgggaag cgtcacaaca acgtcagcca
    10261 taacgaccgc atccttgcgc gtggcacgct tccctgcggc cttctgggcc tcgttcacgc
    10321 gctcgatgcg gctctccacc gtgccccagt tcggctcgcc atcgcgcggc tccacgcgcc
    10381 ccacaacgag cttgccgtcc ttgttcgtgt agtggccgac caccaagtcg ttcttgatgc
    10441 gctcggggtc gatgttgtcg cgctccaggc tgctgcggtc gcggcggtag tgggcaagca
    10501 tgtgcccgac cgatggagcc ttgtatttcg cgatgtgagc catggaaacc tccgaaccgt
    10561 acagacttaa tcgactaaag tctagcacgt tagaccttag ctacgctaag gacgtggcag
    10621 ggccagccct gcacccgctc gggcgttccc cccgagaccc cccagccctt gcccgttcgc
    10681 ggcttcgctc acggccctcg ggccatcggc gcgagcgccg aacggaggga cttcgcccct
    10741 ccgaaccacc cctcaatcta cctgcggttc ggcacacggc acacggcact attagggggg
    10801 ccgtcgtagt ggcccccctg tggtcatttg tcatttgtca tttgtcacat tatttttcgg
    10861 acacattatt tttcggacgt agttagcgaa aaataatgag ccgcccgatt ctgcaggtct
    10921 catccagtcg agacacgggc aaggaaaggg tcgatggagc agagcgtcca tcgaccctta
    10981 atcaagtctg gtctattcgg ttggccgtcg cgtcaattcc gcaatccgtg aacgagtcgg
    11041 cttagatctg cgcgagcgca gaccacgccg ccccgtagtc acgcattgct gcattgaccc
    11101 gactactctt tggcacctga ctgttccacg cacttgcgct tacgttcggc gttcagatag
    11161 ttcccgcgac aatagcgctc gagaatcgag ttgtacccca cgatgatttc gcggtactgc
    11221 tcgggaaact ccctcagcgc cgcagccgtg aaatcgttga tggtctcgaa cctctcgcac
    11281 atgatgaagc cgaccaactc gaagagcatg tcctgcttgt ccttagtcga gagctgttcg
    11341 agctgaccaa tgtcgaagtt gtttccctcg atgcgtgctt cgaccggata cagctccttg
    11401 cccttcttgc gacaggaagc cgtgtcgtga atcagatagt catacgcatg cctgatgttg
    11461 ttgattggct cggctgacga acagcacttc ttgccgtccg cgctcaatcg gttcaagacg
    11521 ttgatgattg ccttgcgtgt cgtgttgccg ccccagacaa caatgacgtg cgcatgcgtc
    11581 ttgcgctgct tgctcttctg gtcatggtct atgtcgtgaa ccgcataagc gaaaggcact
    11641 tgaagaatgt cgtctatctc gtcctgccag tcatcgcgca tgttctctgg gtagcagatg
    11701 ccctcccaga gcttcacctt cgtctcgcca gcgtttgccg ccatgctata atctccttgt
    11761 ctcgaatgcc cct
//
