LOCUS       sequence-375787-       11176 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  E. coli - Eggerthella lenta shuttle plasmid (KanR), Pcadh catechin 
            dehydroxylase promoter-lacZ with transcriptional regulator CadR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11176)
  AUTHORS   Dong X, Guthrie BGH, Alexander M, Noecker C, Ramirez L, Glasser NR, 
            Turnbaugh PJ, Balskus EP
  TITLE     Genetic manipulation of the human gut bacterium Eggerthella lenta 
            reveals a widespread family of transcriptional regulators.
  JOURNAL   Nat Commun. 2022 Dec 9;13(1):7624. doi: 10.1038/s41467-022-33576-3.
  PUBMED    36494336
REFERENCE   2  (bases 1 to 11176)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-12-9"; volume: "13(1):7624. doi"; pages: " 
            10.1038/s41467-022-33576-3"
COMMENT     Sequence Label: pXD70LacZ8-CadR-Pcadh-LacZ
FEATURES             Location/Qualifiers
     source          1..11176
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      24..95
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     rep_origin      complement(276..864)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(356..375)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1035..1826,1827..1895))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1658..1677
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1896..2000)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2068..2086
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    2243..2262
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that start with ""ATG"""
     CDS             4111..7185
                     /codon_start=1
                     /gene="lacZ"
                     /product="beta-galactosidase"
                     /label=lacZ
                     /translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEART
                     DRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNV
                     TYPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYG
                     QDSRLPSEFDLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQ
                     ISDFHVATRFNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIID
                     ERGGYADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIEN
                     GLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLW
                     YTLCDRYGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWS
                     LGNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVP
                     KWSIKKWLSLPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSL
                     IKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQ
                     TIEVTSEYLFRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQ
                     LWLTVRVVQPNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELG
                     NKRWQFNRQSGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWK
                     AAGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDV
                     EVASDTPHPARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPY
                     VFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDG
                     FHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQK"
     primer_bind     complement(4131..4148)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4131..4147)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4140..4162)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     terminator      7203..7230
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             complement(7353..8147)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
ORIGIN
        1 agcttgctgg ggcttttctt tttcaaataa aacgaaaggc tcagtcgaaa gactgggcct
       61 ttcgttttat ctgttgtttg tcggtgaacg ctctctacta gaggagctca tgccggcggc
      121 acctcgctaa cggattcacc actccaagaa ttggagccaa tcaattcttg cggagaactg
      181 tgaatgcgca aaccaaccct tggcagaaca tatccatcgc gtccgccatc tccagcagcc
      241 gcacgcggcg catctcgggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
      301 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
      361 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
      421 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
      481 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
      541 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
      601 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
      661 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca
      721 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
      781 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
      841 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
      901 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
      961 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     1021 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     1081 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     1141 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
     1201 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     1261 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     1321 aatagtttgc gcaacgttgt tgccattgct gcaggcatcg tggtgtcacg ctcgtcgttt
     1381 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     1441 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     1501 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     1561 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     1621 cggcgaccga gttgctcttg cccggcgtca acacgggata ataccgcgcc acatagcaga
     1681 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     1741 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     1801 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     1861 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
     1921 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     1981 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc
     2041 attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtcttcaa
     2101 ggcgaaaaaa ccccgccgaa gcggggtttt ttgcgaaata taatgaccct cttgataacc
     2161 caagagggca ttttttagga acggttattt gattattaat ccggcggcaa ctgaaacgat
     2221 tcggatcctg tattactatt ctgaattcgc ggccgcttct agattcatgc cttcttctgc
     2281 tcgtcgatgg tctgctcgac cagattgagg atctcctgct ggttgtggac gtccatcttc
     2341 gcatagatgt tgcgaaggtg ggtcttcgcg gtggactgcg tgatgacgag cgtgtccgcg
     2401 atatgctgcg cggtgcggcc gcgtgccagc aatacgagga tctcctcctc gcgccccgtc
     2461 agctggtact ggtcggtgag cgcatggcac gcgttctgca gcttgttcgc gtggggaagc
     2521 gcatcgtgaa cgtcgggtgc gtcagggccg ggccattgac cgcggattgg cgtgtggtag
     2581 tcggtgatgc ttcccgaaag cgacgtgatg gctccgcagg cgaacaatac gacaagcgcg
     2641 atgaccgagc tggacacgaa tacgtcgggc gacatatgct ccaatagaag agagctcgcc
     2701 gctttgcccg cggtgaacga gaggaacgcg acgcaccagg gcatcaggta gggtcgtttg
     2761 ctttcgatga ggaagggggc cagcatgata aagaggaaca ccagcttctg cgtgatgttc
     2821 agcaagacga ggtagaggaa tatccacgtc tcgttcacga aggacgacag ccacaaggca
     2881 agaagcacgg tgggtatgag gaacaccttg tacagctcga ggctccgcct gttccagaag
     2941 tactggacga gcacgaggat ggccgctccg ccgcatgcgg tgcccacggc cgtgcccagc
     3001 tggatgatga gcgataccgt cccgccgtct tgcaggggaa tccattggta gtggatatgt
     3061 ccgaacacca cggcatagca agccagcgac agcatcatcg tcacgacgaa tactcgtcga
     3121 ccgcgaggag cctggtccga ggggacgatg agggatcgat cggggcagcc gattcccgcc
     3181 accatgctcc gctccgggct cgcgcaggca tcctcgctcg ggatgtcgtc gcgatccgca
     3241 cgcggcgcgc gcgaacgggc gtattccctg aaatagcaga gcagcaccat cgacacgatg
     3301 gggagcagcg ataccgtgcc gtaagcggca tccaccttca gaaacgcgat gaggctgttc
     3361 attgccgcaa cgacgatcgt tcccaggccc agcacggcgg cgcaggcgcg cgccccgtag
     3421 ggaaagagcg ctttcatcca gagaaacagg atgacgccct ctccaagccc gaatacgatg
     3481 cgcgcgatgg aggatacgat ggggttgtcc tcccatacgc tttgctgcaa gacgacgaac
     3541 atggaagccg acatcaacgc tcctgcgccg atgacgatcg cggcgggaac gtgggtgggc
     3601 gcgtccagct tgcgatacgc aagagccagc atgatcagga gaaggaacga tacgagcgac
     3661 gcgacggcgt ggtagtcgcc gctgatattg acggttctga tcgtcgagag gcggtcgcct
     3721 tcgatggcga gcagcgcgca tccgaagccg atcgaagcga tcgcgattcc cggcacctcg
     3781 cgaatcgcca gcgcaagcgg cgacgatcct cgttcgtctg tcatgcggac ccccttttac
     3841 tcacatcccc atggcgataa ggctcgttcg agcattgtcg tcagtatagc aattcgccga
     3901 taccgcggcg gcgggcggat ggcgaaatac cactctcggt ggtaggtctt tcatgagctc
     3961 gatagtaggg tttctgacct ggttgaacag agttcgcatc agcggtgggg tgctcaactc
     4021 gcgctcgatc tgcatcatgg ttcgcacgga cgcttggtcc ggcccgctcg ttggagtgcg
     4081 ggtgaaaagg gaaccggcag aggaggagct atgaccatga ttacggattc actggccgtc
     4141 gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca
     4201 catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa
     4261 cagttgcgca gcctgaatgg cgaatggcgc tttgcctggt ttccggcacc agaagcggtg
     4321 ccggaaagct ggctggagtg cgatcttcct gaggccgata ctgtcgtcgt cccctcaaac
     4381 tggcagatgc acggttacga tgcgcccatc tacaccaacg tgacctatcc cattacggtc
     4441 aatccgccgt ttgttcccac ggagaatccg acgggttgtt actcgctcac atttaatgtt
     4501 gatgaaagct ggctacagga aggccagacg cgaattattt ttgatggcgt taactcggcg
     4561 tttcatctgt ggtgcaacgg gcgctgggtc ggttacggcc aggacagtcg tttgccgtct
     4621 gaatttgacc tgagcgcatt tttacgcgcc ggagaaaacc gcctcgcggt gatggtgctg
     4681 cgctggagtg acggcagtta tctggaagat caggatatgt ggcggatgag cggcattttc
     4741 cgtgacgtct cgttgctgca taaaccgact acacaaatca gcgatttcca tgttgccact
     4801 cgctttaatg atgatttcag ccgcgctgta ctggaggctg aagttcagat gtgcggcgag
     4861 ttgcgtgact acctacgggt aacagtttct ttatggcagg gtgaaacgca ggtcgccagc
     4921 ggcaccgcgc ctttcggcgg tgaaattatc gatgagcgtg gtggttatgc cgatcgcgtc
     4981 acactacgtc tgaacgtcga aaacccgaaa ctgtggagcg ccgaaatccc gaatctctat
     5041 cgtgcggtgg ttgaactgca caccgccgac ggcacgctga ttgaagcaga agcctgcgat
     5101 gtcggtttcc gcgaggtgcg gattgaaaat ggtctgctgc tgctgaacgg caagccgttg
     5161 ctgattcgag gcgttaaccg tcacgagcat catcctctgc atggtcaggt catggatgag
     5221 cagacgatgg tgcaggatat cctgctgatg aagcagaaca actttaacgc cgtgcgctgt
     5281 tcgcattatc cgaaccatcc gctgtggtac acgctgtgcg accgctacgg cctgtatgtg
     5341 gtggatgaag ccaatattga aacccacggc atggtgccaa tgaatcgtct gaccgatgat
     5401 ccgcgctggc taccggcgat gagcgaacgc gtaacgcgaa tggtgcagcg cgatcgtaat
     5461 cacccgagtg tgatcatctg gtcgctgggg aatgaatcag gccacggcgc taatcacgac
     5521 gcgctgtatc gctggatcaa atctgtcgat ccttcccgcc cggtgcagta tgaaggcggc
     5581 ggagccgaca ccacggccac cgatattatt tgcccgatgt acgcgcgcgt ggatgaagac
     5641 cagcccttcc cggctgtgcc gaaatggtcc atcaaaaaat ggctttcgct acctggagag
     5701 acgcgcccgc tgatcctttg cgaatacgcc cacgcgatgg gtaacagtct tggcggtttc
     5761 gctaaatact ggcaggcgtt tcgtcagtat ccccgtttac agggcggctt cgtctgggac
     5821 tgggtggatc agtcgctgat taaatatgat gaaaacggca acccgtggtc ggcttacggc
     5881 ggtgattttg gcgatacgcc gaacgatcgc cagttctgta tgaacggtct ggtctttgcc
     5941 gaccgcacgc cgcatccagc gctgacggaa gcaaaacacc agcagcagtt tttccagttc
     6001 cgtttatccg ggcaaaccat cgaagtgacc agcgaatacc tgttccgtca tagcgataac
     6061 gagctcctgc actggatggt ggcgctggat ggtaagccgc tggcaagcgg tgaagtgcct
     6121 ctggatgtcg ctccacaagg taaacagttg attgaactgc ctgaactacc gcagccggag
     6181 agcgccgggc aactctggct cacagtacgc gtagtgcaac cgaacgcgac cgcatggtca
     6241 gaagccgggc acatcagcgc ctggcagcag tggcgtctgg cggaaaacct cagtgtgacg
     6301 ctccccgccg cgtcccacgc catcccgcat ctgaccacca gcgaaatgga tttttgcatc
     6361 gagctgggta ataagcgttg gcaatttaac cgccagtcag gctttctttc acagatgtgg
     6421 attggcgata aaaaacaact gctgacgccg ctgcgcgatc agttcacccg tgcaccgctg
     6481 gataacgaca ttggcgtaag tgaagcgacc cgcattgacc ctaacgcctg ggtcgaacgc
     6541 tggaaggcgg cgggccatta ccaggccgaa gcagcgttgt tgcagtgcac ggcagataca
     6601 cttgctgatg cggtgctgat tacgaccgct cacgcgtggc agcatcaggg gaaaacctta
     6661 tttatcagcc ggaaaaccta ccggattgat ggtagtggtc aaatggcgat taccgttgat
     6721 gttgaagtgg cgagcgatac accgcatccg gcgcggattg gcctgaactg ccagctggcg
     6781 caggtagcag agcgggtaaa ctggctcgga ttagggccgc aagaaaacta tcccgaccgc
     6841 cttactgccg cctgttttga ccgctgggat ctgccattgt cagacatgta taccccgtac
     6901 gtcttcccga gcgaaaacgg tctgcgctgc gggacgcgcg aattgaatta tggcccacac
     6961 cagtggcgcg gcgacttcca gttcaacatc agccgctaca gtcaacagca actgatggaa
     7021 accagccatc gccatctgct gcacgcggaa gaaggcacat ggctgaatat cgacggtttc
     7081 catatgggga ttggtggcga cgactcctgg agcccgtcag tatcggcgga attccagctg
     7141 agcgccggtc gctaccatta ccagttggtc tggtgtcaaa aataaactag tcgactcaca
     7201 ctggctcacc ttcgggtggg cctttctgcg tttatatact agagagagaa tataaaaagc
     7261 cagattatta atccggcttt tttattattt aattcctgtt ataaaaaaag gatcaatttt
     7321 gaactctctc ccaaagttga tcccttaacg atctaaaaca attcatccag taaaatataa
     7381 tattttattt tctcccaatc aggcttgatc cccagtaagt caaaaaatag ctcgacatac
     7441 tgttcttccc cgatatcctc cctgatcgac cggacgcaga aggcaatgtc ataccacttg
     7501 tccgccctgc cgcttctccc aagatcaata aagccactta ctttgccatc tttcacaaag
     7561 atgttgctgt ctcccaggtc gccgtgggaa aagacaagtt cctcttcggg cttttccgtc
     7621 tttaaaaaat catacagctc gcgcggatct ttaaatggag tgtcttcttc ccagttttcg
     7681 caatccacat cggccagatc gttattcagt aagtaatcca attcggctaa gcggctgtct
     7741 aagctattcg tatagggaca atccgatatg tcgatggagt gaaagagcct gatgcactcc
     7801 gcatacagct cgataatctt ttcagggctt tgttcatctt catactcttc cgagcaaagg
     7861 acgccatcgg cctcactcat gagcagattg ctccagccat catgccgttc aaagtgcagg
     7921 acctttggaa caggcagctt tccttccagc catagcatca tgtccttttc ccgttccaca
     7981 tcataggtgg tccctttata ccggctgtcc gtcattttta aatataggtt ttcattttct
     8041 cccaccagct tatatacctt agcaggagac attccttccg tatcttttac gcagcggtat
     8101 ttttcgatca gttttttcaa ttccggtgat attctcattt tagccattta ttatttcctt
     8161 cctcttttct acagtattta aagatacccc aagaagctaa ttataacaag acgaactcca
     8221 attcactgtt ccttgcattc taaaacctta aataccagaa aacagctttt tcaaagttgt
     8281 tttcaaagtt ggcgtataac atagtatcga cggagccgat tttgaaacca caattatgat
     8341 agaatttaca agctataagg ttattgtcct gggtttcaag cattagtcca tgcaagtttt
     8401 tatgctttgc ccattctata gatatattga taagaagact ggaatgcccg ctccgccggg
     8461 attattcgaa agcgcatcgt ccaagcgaga ccgtctgcat tcggaaatct cggccggttc
     8521 gcgccgagga agcccatctg gctgcatccc aaatcgacac acaacgcacg gaggccccgg
     8581 cgatcgccgg ggcctccgaa tcatgcgcat cgtggaagcg aagctccgca aaaaaccccg
     8641 cttcagcggg gttttttcgc gtcgcacctc cttgtcgtgt attacaggta ctactataac
     8701 acatcatcta gcccgcccgc gatacgtcct ctgcggtgca gcgtggtcgc ggttccacgc
     8761 atccgccgcc cgctgcgcat cgcgcagcac gtcattaagg ctctgttgcc ttggctgttc
     8821 cctgcgcatc agatattcga gcgggcgcga tattcgactt ctaagctctt ccactttgat
     8881 tcgcattccc gcaactcctg ctctgatgcg gctcgcgagt ccatcgcact taacaacgat
     8941 gttatcgagg attgcgccgc gctcagcttt tcccgcagct tcccagcccc gaacctcggt
     9001 agcgatgggt tgaagctctc caatgtctcg cgcaactccg tccgctcgtt gccgaagaca
     9061 ctccaatcgg tcttgctcat cttcgatatc gagcttcacg tcctcgagct ggctgtcaag
     9121 ctcggcaagg tcaccctcct tgtcggcaat ctcggtaaca agctctgcct tacgcgtttc
     9181 ggcatcattc agtaaagcgg cggcttcttc cctagccgcc gcgacgattc gcgccgcttc
     9241 gtccttcgca ggttcaacca cggccttatc gaccgcacag cgtaccttgt cgatatccac
     9301 ggacttgtcc gtgtatacac gctgcgcccg cgtctcctcg tcaagctcga ttgcagggcg
     9361 gtagcccatg cgcccctcta ggtaatcccc aagctccttg tgcatgctcc ggtagaagct
     9421 gcgggggcac atctgcttgt agttgaaccg tccatctagg atgggcgtga acggcacgtg
     9481 catgtggtcg cgggcgggcg tgccatcctt caacacctcg tccttgtgga cgtacccgcc
     9541 catcatgttc tcgatgccga acttcttcga caggtaccag tacgtgagac ggaagaacct
     9601 gtcctcatcg cccttgcgca cattgtcggg aagcgtcaca acaacgtcag ccataacgac
     9661 cgcatccttg cgcgtggcac gcttccctgc ggccttctgg gcctcgttca cgcgctcgat
     9721 gcggctctcc accgtgcccc agttcggctc gccatcgcgc ggctccacgc gccccacaac
     9781 gagcttgccg tccttgttcg tgtagtggcc gaccaccaag tcgttcttga tgcgctcggg
     9841 gtcgatgttg tcgcgctcca ggctgctgcg gtcgcggcgg tagtgggcaa gcatgtgccc
     9901 gaccgatgga gccttgtatt tcgcgatgtg agccatggaa acctccgaac cgtacagact
     9961 taatcgacta aagtctagca cgttagacct tagctacgct aaggacgtgg cagggccagc
    10021 cctgcacccg ctcgggcgtt ccccccgaga ccccccagcc cttgcccgtt cgcggcttcg
    10081 ctcacggccc tcgggccatc ggcgcgagcg ccgaacggag ggacttcgcc cctccgaacc
    10141 acccctcaat ctacctgcgg ttcggcacac ggcacacggc actattaggg gggccgtcgt
    10201 agtggccccc ctgtggtcat ttgtcatttg tcatttgtca cattattttt cggacacatt
    10261 atttttcgga cgtagttagc gaaaaataat gagccgcccg attctgcagg tctcatccag
    10321 tcgagacacg ggcaaggaaa gggtcgatgg agcagagcgt ccatcgaccc ttaatcaagt
    10381 ctggtctatt cggttggccg tcgcgtcaat tccgcaatcc gtgaacgagt cggcttagat
    10441 ctgcgcgagc gcagaccacg ccgccccgta gtcacgcatt gctgcattga cccgactact
    10501 ctttggcacc tgactgttcc acgcacttgc gcttacgttc ggcgttcaga tagttcccgc
    10561 gacaatagcg ctcgagaatc gagttgtacc ccacgatgat ttcgcggtac tgctcgggaa
    10621 actccctcag cgccgcagcc gtgaaatcgt tgatggtctc gaacctctcg cacatgatga
    10681 agccgaccaa ctcgaagagc atgtcctgct tgtccttagt cgagagctgt tcgagctgac
    10741 caatgtcgaa gttgtttccc tcgatgcgtg cttcgaccgg atacagctcc ttgcccttct
    10801 tgcgacagga agccgtgtcg tgaatcagat agtcatacgc atgcctgatg ttgttgattg
    10861 gctcggctga cgaacagcac ttcttgccgt ccgcgctcaa tcggttcaag acgttgatga
    10921 ttgccttgcg tgtcgtgttg ccgccccaga caacaatgac gtgcgcatgc gtcttgcgct
    10981 gcttgctctt ctggtcatgg tctatgtcgt gaaccgcata agcgaaaggc acttgaagaa
    11041 tgtcgtctat ctcgtcctgc cagtcatcgc gcatgttctc tgggtagcag atgccctccc
    11101 agagcttcac cttcgtctcg ccagcgtttg ccgccatgct ataatctcct tgtctcgaat
    11161 gcccctgctg ccgcca
//
