LOCUS       sequence-375788-       15019 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression vector for an inducible 3xFLAG-TTI1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15019)
  AUTHORS   Wang L, Paudel BB, McKnight RA, Janes KA
  TITLE     Nucleocytoplasmic transport of active HER2 causes fractional escape 
            from the DCIS-like state.
  JOURNAL   Nat Commun. 2023 Apr 13;14(1):2110. doi: 10.1038/s41467-023-37914-x.
  PUBMED    37055441
REFERENCE   2  (bases 1 to 15019)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-04-13"; volume: "14(1):2110. doi"; pages: " 
            10.1038/s41467-023-37914-x"
COMMENT     Sequence Label: pSLIK 3xFLAG-TTI1 zeo
FEATURES             Location/Qualifiers
     source          1..15019
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1680..1913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2098..2142
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2440..2557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    2614..2638
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        2670..2984
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    2678..2696
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2714..2732
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2749..2767
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2785..2803
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2821..2839
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2856..2874
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2892..2910
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2957..2981
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             2993..3058
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     protein_bind    complement(6370..6394)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        6444..7655
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     7492..7511
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             join(7714..8331,8332..8337,8338..8373,8374..8376,8377..8412,
                     8413..8415,8416..8451,8452..8460)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    8498..9071
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(8665..8682)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     8892..8911
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     promoter        9093..9140
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             9159..9533
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     misc_feature    9707..10295
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9760..9780)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(10178..10189)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(10298..10314)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10299..10315)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             10824..11004
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(11030..11047)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    11036..11260
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      11306..11734
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(11393..11412)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     11603..11624
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(11743..11763)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        11748..12077
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      11928..12063
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     11990..12009
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        12125..12172
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             12191..12565
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    12695..12816
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(12732..12751)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     12786..12805
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(12865..12881)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12865..12881)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(12878..12900)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    12889..12905
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(12913..12919,12920..12937,12938..12943))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12958..12979
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(13096..13113)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(13267..13855)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(13347..13366)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(14026..14817,14818..14886))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     14649..14668
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(14887..14991)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaattc tagacagtta tcaacaagtt tgtacaaaaa agcaggcttt
     2641 aaaggaacca attcagtcga ctggatcccg agtttactcc ctatcagtga tagagaacgt
     2701 atgtcgagtt tactccctat cagtgataga gaacgatgtc gagtttactc cctatcagtg
     2761 atagagaacg tatgtcgagt ttactcccta tcagtgatag agaacgtatg tcgagtttac
     2821 tccctatcag tgatagagaa cgtatgtcga gtttatccct atcagtgata gagaacgtat
     2881 gtcgagttta ctccctatca gtgatagaga acgtatgtcg aggtaggcgt gtacggtggg
     2941 aggcctatat aagcagagct cgtttagtga accgtcagat cgccactaga tggactacaa
     3001 agaccatgac ggtgattata aagatcatga catcgattac aaggatgacg atgacaagac
     3061 tagcatggca gtttttgata ctcctgagga ggcctttggt gtcttacgtc cagtctgtgt
     3121 tcagctcaca aagacccaga cagtggagaa tgtggagcat ctgcagacac gactacaagc
     3181 tgtgagtgac agtgcccttc aggaacttca gcagtacatc ctcttccctc tgcgatttac
     3241 cctgaagacc ccaggtccca aaagagagcg tttgatccaa agtgtggtgg aatgcctcac
     3301 atttgtcctt tcttcaacat gtgtgaaaga acaggagctt ctccaggaac tcttttcaga
     3361 actctctgct tgtctgtatt cacccagctc ccaaaaacct gcggctgtgt ccgaggagtt
     3421 gaaattggct gtgatccagg gacttagcac attaatgcac tcagcttatg gggacatcat
     3481 tctgactttt tatgagccct ccattctgcc acgtttagga tttgctgtat ctttactgtt
     3541 aggccttgca gaacaggaga aatcaaagca aattaaaatt gctgccttaa aatgtttaca
     3601 ggttctactc ttgcagtgtg attgtcagga ccatccaagg tcattggatg aacttgaaca
     3661 aaagcagctg ggggatttgt ttgcctcttt tttacctgga atctcaactg cactgaccag
     3721 gcttatcaca ggagacttta aacaaggtca cagcattgtc gtatcttccc taaagatctt
     3781 ttacaagaca gtgagcttca ttatggctga tgaacagctc aaaagaatct caaaggtcca
     3841 agcaaaacct gcagttgagc acagagtagc agagctgatg gtttacaggg aagcagattg
     3901 ggtaaaaaag actggcgaca agttgactat ccttattaaa aagataattg agtgtgtttc
     3961 tgttcaccca cactggaagg tgagactgga actggtagaa cttgtggagg accttctttt
     4021 gaagtgcagt caatcattgg tcgaatgtgc tggtcccctt ctgaaggcct tagtgggact
     4081 agtaaatgat gagagtcctg aaatccaagc ccagtgcaat aaagttctga gacattttgc
     4141 agatcaaaaa gtagtggtgg gcaacaaagc cctcgctgac atcttgtcag aaagcctgca
     4201 ttcccttgcc acatctcttc ctcgcctaat gaactcccaa gatgaccagg gcaaattctc
     4261 tactctttcc ttgttacttg gttatctgaa actcttgggc ccaaaaataa actttgtcct
     4321 caactctgtg gcccatctcc agcggctttc caaagcactc atccaagttc tagagctaga
     4381 cgtggctgac atcaagattg ttgaggaacg gcgttggaac tctgatgatc tgaatgcttc
     4441 tccaaagacc tcagccacac agccttggaa ccgcatccag aggagatatt tccgcttctt
     4501 cactgatgag agaatcttca tgctcttgag gcaggtttgt cagctacttg gttattatgg
     4561 gaatctttat ttgcttgtgg atcactttat ggaactttac catcaatctg tggtttaccg
     4621 gaagcaagct gccatgatcc ttaatgaact ggttacaggg gctgctgggc tggaggttga
     4681 ggatcttcac gaaaaacata ttaaaacaaa cccagaagaa ctgagagaga ttgtgacatc
     4741 tatacttgaa gaatacacaa gtcaagaaaa ttggtatttg gttacctgtc ttgaaactga
     4801 ggaaatggga gaggagctga tgatggagca cccaggcctc caagccatca cgtctggtga
     4861 acacacctgc caagttacat cttttctagc cttctcaaag ccaagtccca ctatttgctc
     4921 catgaacagt aacatctggc aaatatgcat tcagttggaa ggaattggcc agtttgcata
     4981 tgcactagga aaagacttct gtttgctctt gatgtcagcc ctttatccag tactggagaa
     5041 ggctggagac caaaccctac tcattagtca ggtggctacc agcaccatga tggacgtttg
     5101 ccgtgcttgt ggctacgact ccctgcagca cctgatcaat caaaattcag actatttagt
     5161 gaatgggatc tctttaaatc tgcgtcatct ggctctgcat cctcataccc caaaggtcct
     5221 ggaagtcatg ctgcggaact cagatgctaa cctgcttcct ttggtggcag atgtggttca
     5281 agatgtcttg gccaccctgg accaatttta cgataagaga gctgcttcct ttgtcagcgt
     5341 tctgcatgct ctgatggcag cattagccca gtggttccca gacacaggta atcttgggca
     5401 cctccaagag caaagtttag gagaagaggg aagtcatttg aaccaaagac cagcagctct
     5461 tgagaagagc accaccacag ctgaagacat cgaacagttt ttgctgaact acctcaaaga
     5521 gaaggatgtg gcagatggaa atgtctcgga ttttgataat gaagaagagg aacagtcagt
     5581 ccctcccaaa gtggatgaga atgacacccg tccagatgtg gagccaccac tgccattgca
     5641 gatccaaata gccatggacg tgatggaacg ctgcatccac ttgttgtcag ataaaaatct
     5701 gcaaatccgc ctgaaggtct tggatgtgct ggatctgtgt gtggttgttc ttcagtccca
     5761 caaaaaccag ctgcttccct tggctcatca ggcctggccc tcgctcgttc accgactcac
     5821 acgggacgcc cccctggcag tgcttagagc cttcaaggtt ttacgtaccc tgggaagcaa
     5881 gtgtggtgac tttcttcgca gccggttctg caaagatgtc ctgccaaagc tggctggctc
     5941 cctagtcacc caggccccca tcagtgccag ggctggacca gtttactcgc acacgctggc
     6001 cttcaagttg cagctggctg tcttacaggg cctgggcccc ctctgtgaga gactggacct
     6061 aggtgagggt gacctgaata aagtggctga tgcctgcttg atttacctca gtgtcaaaca
     6121 gcccgtgaaa ttacaagagg ctgccaggag cgtcttcctc cacttgatga aggtggaccc
     6181 agactccacc tggttcctcc tgaacgagct ttactgcccc gtgcagttca cacctcccca
     6241 ccccagcctc caccctgtgc agctgcacgg ggccagcggg cagcagaacc cctacacgac
     6301 caacgtgctc cagctgctca aggagctgca gtgacaattg gaagactaat gcgttttcga
     6361 gatatctaga cccagctttc ttgtacaaag tggttgataa catctcgagc cataaagatg
     6421 gttaattaac ccacccaaga tctggcctcc gcgccgggtt ttggcgcctc ccgcgggcgc
     6481 ccccctcctc acggcgagcg ctgccacgtc agacgaaggg cgcagcgagc gtcctgatcc
     6541 ttccgcccgg acgctcagga cagcggcccg ctgctcataa gactcggcct tagaacccca
     6601 gtatcagcag aaggacattt taggacggga cttgggtgac tctagggcac tggttttctt
     6661 tccagagagc ggaacaggcg aggaaaagta gtcccttctc ggcgattctg cggagggatc
     6721 tccgtggggc ggtgaacgcc gatgattata taaggacgcg ccgggtgtgg cacagctagt
     6781 tccgtcgcag ccgggatttg ggtcgcggtt cttgtttgtg gatcgctgtg atcgtcactt
     6841 ggtgagtagc gggctgctgg gctggccggg gctttcgtgg ccgccgggcc gctcggtggg
     6901 acggaagcgt gtggagagac cgccaagggc tgtagtctgg gtccgcgagc aaggttgccc
     6961 tgaactgggg gttgggggga gcgcagcaaa atggcggctg ttcccgagtc ttgaatggaa
     7021 gacgcttgtg aggcgggctg tgaggtcgtt gaaacaaggt ggggggcatg gtgggcggca
     7081 agaacccaag gtcttgaggc cttcgctaat gcgggaaagc tcttattcgg gtgagatggg
     7141 ctggggcacc atctggggac cctgacgtga agtttgtcac tgactggaga actcggtttg
     7201 tcgtctgttg cgggggcggc agttatggcg gtgccgttgg gcagtgcacc cgtacctttg
     7261 ggagcgcgcg ccctcgtcgt gtcgtgacgt cacccgttct gttggcttat aatgcagggt
     7321 ggggccacct gccggtaggt gtgcggtagg cttttctccg tcgcaggacg cagggttcgg
     7381 gcctagggta ggctctcctg aatcgacagg cgccggacct ctggtgaggg gagggataag
     7441 tgaggcgtca gtttctttgg tcggttttat gtacctatct tcttaagtag ctgaagctcc
     7501 ggttttgaac tatgcgctcg gggttggcga gtgtgttttg tgaagttttt taggcacctt
     7561 ttgaaatgta atcatttggg tcaatatgta attttcagtg ttagactagt aaattgtccg
     7621 ctaaattctg gccgtttttg gcttttttgt tagacgaagc ttggtaccga gctcggatct
     7681 ccaccccgta ccggtcctgc agtcgaattc accatgtcta gactggacaa gagcaaagtc
     7741 ataaacggag ctctggaatt actcaatggt gtcggtatcg aaggcctgac gacaaggaaa
     7801 ctcgctcaaa agctgggagt tgagcagcct accctgtact ggcacgtgaa gaacaagcgg
     7861 gccctgctcg atgccctgcc aatcgagatg ctggacaggc atcataccca cttctgcccc
     7921 ctggaaggcg agtcatggca agactttctg cggaacaacg ccaagtcata ccgctgtgct
     7981 ctcctctcac atcgcgacgg ggctaaagtg catctcggca cccgcccaac agagaaacag
     8041 tacgaaaccc tggaaaatca gctcgcgttc ctgtgtcagc aaggcttctc cctggagaac
     8101 gcactgtacg ctctgtccgc cgtgggccac tttacactgg gctgcgtatt ggaggaacag
     8161 gagcatcaag tagcaaaaga ggaaagagag acacctacca ccgattctat gcccccactt
     8221 ctgagacaag caattgagct gttcgaccgg cagggagccg aacctgcctt ccttttcggc
     8281 ctggaactaa tcatatgtgg cctggagaaa cagctaaagt gcgaaagcgg cgggccgacc
     8341 gacgcccttg acgattttga cttagacatg ctcccagccg atgcccttga cgactttgac
     8401 cttgatatgc tgcctgctga cgctcttgac gattttgacc ttgacatgct ccccgggtaa
     8461 ctaagtaagg atccgcggcc gcactagagg aattccgccc ctctcccccc ccccccccta
     8521 acgttactgg ccgaagccgc ttggaataag gccggtgtgc gtttgtctat atgttatttt
     8581 ccaccatatt gccgtctttt ggcaatgtga gggcccggaa acctggccct gtcttcttga
     8641 cgagcattcc taggggtctt tcccctctcg ccaaaggaat gcaaggtctg ttgaatgtcg
     8701 tgaaggaagc agttcctctg gaagcttctt gaagacaaac aacgtctgta gcgacccttt
     8761 gcaggcagcg gaacccccca cctggcgaca ggtgcctctg cggccaaaag ccacgtgtat
     8821 aagatacacc tgcaaaggcg gcacaacccc agtgccacgt tgtgagttgg atagttgtgg
     8881 aaagagtcaa atggctctcc tcaagcgtat tcaacaaggg gctgaaggat gcccagaagg
     8941 taccccattg tatgggatct gatctggggc ctcggtgcac atgctttaca tgtgtttagt
     9001 cgaggttaaa aaaacgtcta ggccccccga accacgggga cgtggttttc ctttgaaaaa
     9061 cacgatgata agcttaccgg tacgcgtgat gtgttgacaa ttaatcatcg gcatagtata
     9121 tcggcatagt ataatacgac aaggtgagga actaaaccat ggccaagttg accagtgccg
     9181 ttccggtgct caccgcgcgc gacgtcgccg gagcggtcga gttctggacc gaccggctcg
     9241 ggttctcccg ggacttcgtg gaggacgact tcgccggtgt ggtccgggac gacgtgaccc
     9301 tgttcatcag cgcggtccag gaccaggtgg tgccggacaa caccctggcc tgggtgtggg
     9361 tgcgcggcct ggacgagctg tacgccgagt ggtcggaggt cgtgtccacg aacttccggg
     9421 acgcctccgg gccggccatg accgagatcg gcgagcagcc gtgggggcgg gagttcgccc
     9481 tgcgcgaccc ggccggcaac tgcgtgcact tcgtggccga ggagcaggac tgacacatct
     9541 gtacaagtaa agcggccgcg actctagatc ataatcagcc ataccacatt tgtagaggtt
     9601 ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca
     9661 attgttgttg tttagtccct cccaattcga tatcaagctt atcgataatc aacctctgga
     9721 ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg
     9781 tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt
     9841 ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag
     9901 gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc
     9961 caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga
    10021 actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa
    10081 ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac
    10141 ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct
    10201 tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca
    10261 gacgagtcgg atctcccttt gggccgcctc cccgcatcga taccgtcgac ctcgatcgag
    10321 acctagaaaa acatggagca atcacaagta gcaatacagc agctaccaat gctgattgtg
    10381 cctggctaga agcacaagag gaggaggagg tgggttttcc agtcacacct caggtacctt
    10441 taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg
    10501 gactggaagg gctaattcac tcccaacgaa gacaagatat ccttgatctg tggatctacc
    10561 acacacaagg ctacttccct gattggcaga actacacacc agggccaggg atcagatatc
    10621 cactgacctt tggatggtgc tacaagctag taccagttga gcaagagaag gtagaagaag
    10681 ccaatgaagg agagaacacc cgcttgttac accctgtgag cctgcatggg atggatgacc
    10741 cggagagaga agtattagag tggaggtttg acagccgcct agcatttcat cacatggccc
    10801 gagagctgca tccggactgt actgggtctc tctggttaga ccagatctga gcctgggagc
    10861 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
    10921 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
    10981 agtcagtgtg gaaaatctct agcagggccc gtttaaaccc gctgatcagc ctcgactgtg
    11041 ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa
    11101 ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt
    11161 aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa
    11221 gacaatagca ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc
    11281 agctggggct ctagggggta tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt
    11341 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
    11401 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
    11461 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
    11521 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
    11581 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct
    11641 atctcggtct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa
    11701 aatgagctga tttaacaaaa atttaacgcg aattaattct gtggaatgtg tgtcagttag
    11761 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
    11821 agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
    11881 tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa
    11941 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag
    12001 aggccgaggc cgcctctgcc tctgagctat tccagaagta gtgaggaggc ttttttggag
    12061 gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc cattttcgga tctgatcagc
    12121 acgtgttgac aattaatcat cggcatagta tatcggcata gtataatacg acaaggtgag
    12181 gaactaaacc atggccaagt tgaccagtgc cgttccggtg ctcaccgcgc gcgacgtcgc
    12241 cggagcggtc gagttctgga ccgaccggct cgggttctcc cgggacttcg tggaggacga
    12301 cttcgccggt gtggtccggg acgacgtgac cctgttcatc agcgcggtcc aggaccaggt
    12361 ggtgccggac aacaccctgg cctgggtgtg ggtgcgcggc ctggacgagc tgtacgccga
    12421 gtggtcggag gtcgtgtcca cgaacttccg ggacgcctcc gggccggcca tgaccgagat
    12481 cggcgagcag ccgtgggggc gggagttcgc cctgcgcgac ccggccggca actgcgtgca
    12541 cttcgtggcc gaggagcagg actgacacgt gctacgagat ttcgattcca ccgccgcctt
    12601 ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg
    12661 cggggatctc atgctggagt tcttcgccca ccccaacttg tttattgcag cttataatgg
    12721 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
    12781 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctgtatac cgtcgacctc
    12841 tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct
    12901 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
    12961 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
    13021 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
    13081 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
    13141 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
    13201 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
    13261 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
    13321 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
    13381 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
    13441 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
    13501 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
    13561 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
    13621 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
    13681 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
    13741 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
    13801 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
    13861 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
    13921 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
    13981 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
    14041 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
    14101 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
    14161 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
    14221 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
    14281 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
    14341 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
    14401 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
    14461 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
    14521 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
    14581 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
    14641 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
    14701 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
    14761 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
    14821 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
    14881 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
    14941 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
    15001 ccgaaaagtg ccacctgac
//
