LOCUS       sequence-375790-        8465 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Retroviral expression vector for NGF-Erbb2-2xFKBP-HA construct.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8465)
  AUTHORS   Wang L, Paudel BB, McKnight RA, Janes KA
  TITLE     Nucleocytoplasmic transport of active HER2 causes fractional escape 
            from the DCIS-like state.
  JOURNAL   Nat Commun. 2023 Apr 13;14(1):2110. doi: 10.1038/s41467-023-37914-x.
  PUBMED    37055441
REFERENCE   2  (bases 1 to 8465)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-04-13"; volume: "14(1):2110. doi"; pages: " 
            10.1038/s41467-023-37914-x"
COMMENT     Sequence Label: pBabe NGF-Erbb2-2xFKBP-HA hygro
FEATURES             Location/Qualifiers
     source          1..8465
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             98..567
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    632..831
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1032..1448
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1378..1400
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1416..1432
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     CDS             4057..4377
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
     CDS             4384..4704
                     /codon_start=1
                     /product="F36V mutant of FK506-binding protein FKBP12"
                     /label=DmrB
                     /note="binds synthetic ligands that do not bind wild-type 
                     FKBP"
                     /translation="GVQVETTSPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
     CDS             4711..4737
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(4752..4772)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4757..5086
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4937..5072
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5160..6185
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             5160..6185
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     primer_bind     6702..6721
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     LTR             6983..7452
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     CDS             complement(join(7567..8358,8359..8427))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8190..8209
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(8428..8465,1..67))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
       61 ttccgcgcac atttccccga aaagtgccac ctgcagcctg aatatgggcc aaacaggata
      121 tctgtggtaa gcagttcctg ccccggctca gggccaagaa cagatggaac agctgaatat
      181 gggccaaaca ggatatctgt ggtaagcagt tcctgccccg gctcagggcc aagaacagat
      241 ggtccccaga tgcggtccag ccctcagcag tttctagaga accatcagat gtttccaggg
      301 tgccccaagg acctgaaatg accctgtgcc ttatttgaac taaccaatca gttcgcttct
      361 cgcttctgtt cgcgcgcttc tgctccccga gctcaataaa agagcccaca acccctcact
      421 cggggcgcca gtcctccgat tgactgagtc gcccgggtac ccgtgtatcc aataaaccct
      481 cttgcagttg catccgactt gtggtctcgc tgttccttgg gagggtctcc tctgagtgat
      541 tgactacccg tcagcggggg tctttcattt gggggctcgt ccgggatcgg gagacccctg
      601 cccagggacc accgacccac caccgggagg caagctggcc agcaacttat ctgtgtctgt
      661 ccgattgtct agtgtctatg actgatttta tgcgcctgcg tcggtactag ttagctaact
      721 agctctgtat ctggcggacc cgtggtggaa ctgacgagtt ctgaacaccc ggccgcaacc
      781 ctgggagacg tcccagggac tttgggggcc gtttttgtgg cccgacctga ggaagggagt
      841 cgatgtggaa tccgaccccg tcaggatatg tggttctggt aggagacgag aacctaaaac
      901 agttcccgcc tccgtctgaa tttttgcttt cggtttggaa ccgaagccgc gcgtcttgtc
      961 tgctgcagcg ctgcagcatc gttctgtgtt gtctctgtct gactgtgttt ctgtatttgt
     1021 ctgaaaatta gggccagact gttaccactc ccttaagttt gaccttaggt cactggaaag
     1081 atgtcgagcg gatcgctcac aaccagtcgg tagatgtcaa gaagagacgt tgggttacct
     1141 tctgctctgc agaatggcca acctttaacg tcggatggcc gcgagacggc acctttaacc
     1201 gagacctcat cacccaggtt aagatcaagg tcttttcacc tggcccgcat ggacacccag
     1261 accaggtccc ctacatcgtg acctgggaag ccttggcttt tgacccccct ccctgggtca
     1321 agccctttgt acaccctaag cctccgcctc ctcttcctcc atccgccccg tctctccccc
     1381 ttgaacctcc tcgttcgacc ccgcctcgat cctcccttta tccagccctc actccttctc
     1441 taggcgccgg ccggatccca gtgtggtggt acgtaggaat tgatgggggc aggtgccacc
     1501 ggccgcgcca tggacgggcc gcgcctgctg ctgttgctgc ttctgggggt gtcccttgga
     1561 ggtgccaagg aggcatgccc cacaggcctg tacacacaca gcggtgagtg ctgcaaagcc
     1621 tgcaacctgg gcgagggtgt ggcccagccc tgtggagcca accagaccgt gtgtgagccc
     1681 tgcctggaca gcgtgacgtt ctccgacgtg gtgagcgcga ccgagccgtg caagccgtgc
     1741 accgagtgcg tggggctcca gagcatgtcg gcgccgtgcg tggaggccga cgacgccgtg
     1801 tgccgctgcg cctacggcta ctaccaggat gagacgactg ggcgctgcga ggcgtgccgc
     1861 gtgtgcgagg cgggctcggg cctcgtgttc tcctgccagg acaagcagaa caccgtgtgc
     1921 gaggagtgcc ccgacggcac gtattccgac gaggccaacc acgtggaccc gtgcctgccc
     1981 tgcaccgtgt gcgaggacac cgagcgccag ctccgcgagt gcacacgctg ggccgacgcc
     2041 gagtgcgagg agatccctgg ccgttggatt acacggtcca cacccccaga gggctcggac
     2101 agcacagccc ccagcaccca ggagcctgag gcacctccag aacaagacct catagccagc
     2161 acggtggcag gtgtggtgac cacagtgatg ggcagctccc agcccgtggt gacccgaggc
     2221 accaccgaca acctcatccc tgtctattgc tccatcctgg ctgctgtggt tgtgggtctt
     2281 gtggcctaca tagccttcaa gaggtctaga aaacgaagga gacagaagat ccggaagtat
     2341 acgatgcgta ggctgctgca ggaaactgag ttagtggagc cgctgacgcc cagcggagca
     2401 atgcccaacc aggctcagat gcggatccta aaagagacgg agctaaggaa ggtgaaggtg
     2461 cttggatcag gagcttttgg cactgtctac aagggcatct ggatcccaga tggggagaat
     2521 gtgaaaatcc ccgtggctat caaggtgttg agagaaaaca catctcctaa agccaacaaa
     2581 gaaattctag atgaagcgta tgtgatggct ggtgtgggtt ctccgtatgt gtcccgcctc
     2641 ctgggcatct gcctgacatc cacagtacag ctggtgacac agcttatgcc ctacggctgc
     2701 cttctggacc atgtccgaga acaccgaggt cgcctaggct cccaggacct gctcaactgg
     2761 tgtgttcaga ttgccaaggg gatgagctac ctggaggacg tgcggcttgt acacagggac
     2821 ctggctgccc ggaatgtgct agtcaagagt cccaaccacg tcaagattac agatttcggg
     2881 ctggctcggc tgctggacat tgatgagaca gagtaccatg cagatggggg caaggtgccc
     2941 atcaaatgga tggcattgga atctattctc agacgccggt tcacccatca gagtgatgtg
     3001 tggagctatg gagtgactgt gtgggagctg atgacttttg gggccaaacc ttacgatgga
     3061 atcccagccc gggagatccc tgatttgctg gagaagggag aacgcctacc tcagcctcca
     3121 atctgcacca ttgatgtcta catgattatg gtcaaatgtt ggatgattga ctctgaatgt
     3181 cgcccgagat tccgggagtt ggtgtcagaa ttttcacgta tggcgaggga cccccagcgt
     3241 tttgtggtca tccagaacga ggacttgggc ccatccagcc ccatggacag taccttctac
     3301 cgttcactgc tggaagatga tgacatgggt gacctggtag acgctgaaga gtatctggtg
     3361 ccccagcagg gattcttctc cccggaccct accccaggca ctgggagcac agcccataga
     3421 aggcaccgca gctcgtccac caggagtgga ggtggtgagc tgacactggg cctggagccc
     3481 tcggaagaag ggccccccag atctccactg gctccctcgg aaggggctgg ctccgatgtg
     3541 tttgatggtg acctggcaat gggggtaacc aaagggctgc agagcctctc tccacatgac
     3601 ctcagccctc tacagcggta cagcgaggac cccacattac ctctgccccc cgagactgat
     3661 ggctatgttg ctcccctggc ctgcagcccc cagcccgagt atgtgaacca atcagaggtt
     3721 cagcctcagc ctcctttaac cccagagggt cctctgcctc ctgtccggcc tgctggtgct
     3781 actctagaaa gacccaagac tctctctcct gggaagaatg gggttgtcaa agacgttttt
     3841 gccttcgggg gtgctgtgga gaaccctgaa tacttagtac cgagagaagg cactgcctct
     3901 ccgccccacc cttctcctgc cttcagccca gcctttgaca acctctatta ctgggaccag
     3961 aactcatcgg agcaggggcc tccaccaagt aactttgaag ggacccccac tgcagagaac
     4021 cctgagtacc taggcctgga tgtacctgta actagaggag tgcaggtgga aaccatctcc
     4081 ccgggagacg ggcgcacctt ccccaagcgc ggccagacct gcgtggtgca ctacaccggg
     4141 atgcttgaag atggaaagaa atttgattcc tcccgggaca gaaacaagcc ctttaagttt
     4201 atgctaggca agcaggaggt gatccgaggc tgggaagaag gggttgccca gatgagtgtg
     4261 ggtcagagag ccaaactgac tatatctcca gattatgcct atggtgccac tgggcaccca
     4321 ggcatcatcc caccacatgc cactctcgtc ttcgatgtgg agcttctaaa actggaagtc
     4381 gagggcgtgc aggtggagac tacctcccca ggagacgggc gcaccttccc caagcgcggc
     4441 cagacctgcg tggtgcacta caccgggatg cttgaagatg gaaagaaatt tgattcctcc
     4501 cgggacagaa acaagccctt taagtttatg ctaggcaagc aggaggtgat ccgaggctgg
     4561 gaagaagggg ttgcccagat gagtgtgggt cagagagcca aactgactat atctccagat
     4621 tatgcctatg gtgccactgg gcacccaggc atcatcccac cacatgccac tctcgtcttc
     4681 gatgtggagc ttctaaaact ggaaactagt tatccgtacg acgtaccaga ctacgcataa
     4741 gtcgaccctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg
     4801 cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg
     4861 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc
     4921 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
     4981 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt
     5041 ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc ttgccagctg
     5101 gggcgccctc tggtaaggtt gggaagccct gcagatctga tcccgggggg caatgagata
     5161 tgaaaaagcc tgaactcacc gcgacgtctg tcgagaagtt tctgatcgaa aagttcgaca
     5221 gcgtctccga cctgatgcag ctctcggagg gcgaagaatc tcgtgctttc agcttcgatg
     5281 taggagggcg tggatatgtc ctgcgggtaa atagctgcgc cgatggtttc tacaaagatc
     5341 gttatgttta tcggcacttt gcatcggccg cgctcccgat tccggaagtg cttgacattg
     5401 gggaatttag cgagagcctg acctattgca tctcccgccg tgcacagggt gtcacgttgc
     5461 aagacctgcc tgaaaccgaa ctgcccgctg ttctgcagcc ggtcgcggag gccatggatg
     5521 cgatcgctgc ggccgatctt agccagacga gcgggttcgg cccattcgga ccgcaaggaa
     5581 tcggtcaata cactacatgg cgtgatttca tatgcgcgat tgctgatccc catgtgtatc
     5641 actggcaaac tgtgatggac gacaccgtca gtgcgtccgt cgcgcaggct ctcgatgagc
     5701 tgatgctttg ggccgaggac tgccccgaag tccggcacct cgtgcacgcg gatttcggct
     5761 ccaacaatgt cctgacggac aatggccgca taacagcggt cattgactgg agcgaggcga
     5821 tgttcgggga ttcccaatac gaggtcgcca acatcttctt ctggaggccg tggttggctt
     5881 gtatggagca gcagacgcgc tacttcgagc ggaggcatcc ggagcttgca ggatcgccgc
     5941 ggctccgggc gtatatgctc cgcattggtc ttgaccaact ctatcagagc ttggttgacg
     6001 gcaatttcga tgatgcagct tgggcgcagg gtcgatgcga cgcaatcgtc cgatccggag
     6061 ccgggactgt cgggcgtaca caaatcgccc gcagaagcgc ggccgtctgg accgatggct
     6121 gtgtagaagt actcgccgat agtggaaacc gacgccccag cactcgtccg agggcaaagg
     6181 aatagagtag atgccgaccg ggatctatcg atgcggcggg tgtggtggtt acgcgtaatc
     6241 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     6301 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
     6361 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     6421 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     6481 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     6541 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     6601 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     6661 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     6721 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     6781 ggggggcgga gcctatggaa aaacgccagc aacgcatcga taaaataaaa gattttattt
     6841 agtctccaga aaaagggggg aatgaaagac cccacctgta ggtttggcaa gctagcttaa
     6901 gtaacgccat tttgcaaggc atggaaaaat acataactga gaatagagaa gttcagatca
     6961 aggtcaggaa cagatggaac agctgaatat gggccaaaca ggatatctgt ggtaagcagt
     7021 tcctgccccg gctcagggcc aagaacagat ggaacagctg aatatgggcc aaacaggata
     7081 tctgtggtaa gcagttcctg ccccggctca gggccaagaa cagatggtcc ccagatgcgg
     7141 tccagccctc agcagtttct agagaaccat cagatgtttc cagggtgccc caaggacctg
     7201 aaatgaccct gtgccttatt tgaactaacc aatcagttcg cttctcgctt ctgttcgcgc
     7261 gcttctgctc cccgagctca ataaaagagc ccacaacccc tcactcgggg cgccagtcct
     7321 ccgattgact gagtcgcccg ggtacccgtg tatccaataa accctcttgc agttgcatcc
     7381 gacttgtggt ctcgctgttc cttgggaggg tctcctctga gtgattgact acccgtcagc
     7441 gggggtcttt cacatgcagc atgtatcaaa attaatttgg ttttttttct taagtattta
     7501 cattaaatgg ccatagtgcg gccgcaaatc aatctaaagt atatatgagt aaacttggtc
     7561 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     7621 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     7681 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
     7741 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     7801 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     7861 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
     7921 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
     7981 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
     8041 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
     8101 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
     8161 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
     8221 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
     8281 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
     8341 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
     8401 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
     8461 tcagg
//
