LOCUS       sequence-375796-        9599 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  E. coli - Eggerthella lenta shuttle plasmid (KanR), Phcdh 
            hydrocaffeic acid dehydroxylase promoter-lacZ.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9599)
  AUTHORS   Dong X, Guthrie BGH, Alexander M, Noecker C, Ramirez L, Glasser NR, 
            Turnbaugh PJ, Balskus EP
  TITLE     Genetic manipulation of the human gut bacterium Eggerthella lenta 
            reveals a widespread family of transcriptional regulators.
  JOURNAL   Nat Commun. 2022 Dec 9;13(1):7624. doi: 10.1038/s41467-022-33576-3.
  PUBMED    36494336
REFERENCE   2  (bases 1 to 9599)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-12-9"; volume: "13(1):7624. doi"; pages: " 
            10.1038/s41467-022-33576-3"
COMMENT     Sequence Label: pXD70LacZ9-Phcdh-LacZ
FEATURES             Location/Qualifiers
     source          1..9599
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      57..128
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     rep_origin      complement(309..897)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(389..408)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1068..1859,1860..1928))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1691..1710
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1929..2033)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2101..2119
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    2276..2295
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that start with ""ATG"""
     CDS             2567..5641
                     /codon_start=1
                     /gene="lacZ"
                     /product="beta-galactosidase"
                     /label=lacZ
                     /translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEART
                     DRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNV
                     TYPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYG
                     QDSRLPSEFDLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQ
                     ISDFHVATRFNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIID
                     ERGGYADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIEN
                     GLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLW
                     YTLCDRYGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWS
                     LGNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVP
                     KWSIKKWLSLPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSL
                     IKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQ
                     TIEVTSEYLFRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQ
                     LWLTVRVVQPNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELG
                     NKRWQFNRQSGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWK
                     AAGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDV
                     EVASDTPHPARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPY
                     VFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDG
                     FHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQK"
     primer_bind     complement(2587..2604)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2587..2603)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2596..2618)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     terminator      5659..5686
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             complement(5809..6603)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
ORIGIN
        1 atctccttgt ctcgaatgcc cctgctgccg ccaagcttgc tggggctttt ctttttcaaa
       61 taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga
      121 acgctctcta ctagaggagc tcatgccggc ggcacctcgc taacggattc accactccaa
      181 gaattggagc caatcaattc ttgcggagaa ctgtgaatgc gcaaaccaac ccttggcaga
      241 acatatccat cgcgtccgcc atctccagca gccgcacgcg gcgcatctcg ggccgcgttg
      301 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
      361 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
      421 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
      481 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
      541 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
      601 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
      661 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
      721 tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag
      781 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
      841 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
      901 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
      961 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     1021 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     1081 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     1141 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     1201 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
     1261 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     1321 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     1381 gctgcaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     1441 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     1501 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     1561 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     1621 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     1681 tcaacacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
     1741 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
     1801 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
     1861 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
     1921 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     1981 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
     2041 ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa
     2101 aataggcgta tcacgaggcc ctttcgtctt caaggcgaaa aaaccccgcc gaagcggggt
     2161 tttttgcgaa atataatgac cctcttgata acccaagagg gcatttttta ggaacggtta
     2221 tttgattatt aatccggcgg caactgaaac gattcggatc ctgtattact attctgaatt
     2281 cgcggccgct tctagatggc agtctccttc gttcacaggt ttcttcccgt tgccgcacgc
     2341 ccggatgacg ggcgcgaacg gcaacctctt cgctgccatc aagtataggt tgggcgcgaa
     2401 aagcaaaata atcctttggg gatgatatcg gcgcgtttca tccatccacc ccttatgatt
     2461 tgggcttttg ctccgttcct agaatgggga aaccggttcc ggcgtatggt cgcgccgggt
     2521 tccggcagag acgaacgaag cgagaaaagg aagagaggag gggcacatga ccatgattac
     2581 ggattcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact
     2641 taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac
     2701 cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tggcgctttg cctggtttcc
     2761 ggcaccagaa gcggtgccgg aaagctggct ggagtgcgat cttcctgagg ccgatactgt
     2821 cgtcgtcccc tcaaactggc agatgcacgg ttacgatgcg cccatctaca ccaacgtgac
     2881 ctatcccatt acggtcaatc cgccgtttgt tcccacggag aatccgacgg gttgttactc
     2941 gctcacattt aatgttgatg aaagctggct acaggaaggc cagacgcgaa ttatttttga
     3001 tggcgttaac tcggcgtttc atctgtggtg caacgggcgc tgggtcggtt acggccagga
     3061 cagtcgtttg ccgtctgaat ttgacctgag cgcattttta cgcgccggag aaaaccgcct
     3121 cgcggtgatg gtgctgcgct ggagtgacgg cagttatctg gaagatcagg atatgtggcg
     3181 gatgagcggc attttccgtg acgtctcgtt gctgcataaa ccgactacac aaatcagcga
     3241 tttccatgtt gccactcgct ttaatgatga tttcagccgc gctgtactgg aggctgaagt
     3301 tcagatgtgc ggcgagttgc gtgactacct acgggtaaca gtttctttat ggcagggtga
     3361 aacgcaggtc gccagcggca ccgcgccttt cggcggtgaa attatcgatg agcgtggtgg
     3421 ttatgccgat cgcgtcacac tacgtctgaa cgtcgaaaac ccgaaactgt ggagcgccga
     3481 aatcccgaat ctctatcgtg cggtggttga actgcacacc gccgacggca cgctgattga
     3541 agcagaagcc tgcgatgtcg gtttccgcga ggtgcggatt gaaaatggtc tgctgctgct
     3601 gaacggcaag ccgttgctga ttcgaggcgt taaccgtcac gagcatcatc ctctgcatgg
     3661 tcaggtcatg gatgagcaga cgatggtgca ggatatcctg ctgatgaagc agaacaactt
     3721 taacgccgtg cgctgttcgc attatccgaa ccatccgctg tggtacacgc tgtgcgaccg
     3781 ctacggcctg tatgtggtgg atgaagccaa tattgaaacc cacggcatgg tgccaatgaa
     3841 tcgtctgacc gatgatccgc gctggctacc ggcgatgagc gaacgcgtaa cgcgaatggt
     3901 gcagcgcgat cgtaatcacc cgagtgtgat catctggtcg ctggggaatg aatcaggcca
     3961 cggcgctaat cacgacgcgc tgtatcgctg gatcaaatct gtcgatcctt cccgcccggt
     4021 gcagtatgaa ggcggcggag ccgacaccac ggccaccgat attatttgcc cgatgtacgc
     4081 gcgcgtggat gaagaccagc ccttcccggc tgtgccgaaa tggtccatca aaaaatggct
     4141 ttcgctacct ggagagacgc gcccgctgat cctttgcgaa tacgcccacg cgatgggtaa
     4201 cagtcttggc ggtttcgcta aatactggca ggcgtttcgt cagtatcccc gtttacaggg
     4261 cggcttcgtc tgggactggg tggatcagtc gctgattaaa tatgatgaaa acggcaaccc
     4321 gtggtcggct tacggcggtg attttggcga tacgccgaac gatcgccagt tctgtatgaa
     4381 cggtctggtc tttgccgacc gcacgccgca tccagcgctg acggaagcaa aacaccagca
     4441 gcagtttttc cagttccgtt tatccgggca aaccatcgaa gtgaccagcg aatacctgtt
     4501 ccgtcatagc gataacgagc tcctgcactg gatggtggcg ctggatggta agccgctggc
     4561 aagcggtgaa gtgcctctgg atgtcgctcc acaaggtaaa cagttgattg aactgcctga
     4621 actaccgcag ccggagagcg ccgggcaact ctggctcaca gtacgcgtag tgcaaccgaa
     4681 cgcgaccgca tggtcagaag ccgggcacat cagcgcctgg cagcagtggc gtctggcgga
     4741 aaacctcagt gtgacgctcc ccgccgcgtc ccacgccatc ccgcatctga ccaccagcga
     4801 aatggatttt tgcatcgagc tgggtaataa gcgttggcaa tttaaccgcc agtcaggctt
     4861 tctttcacag atgtggattg gcgataaaaa acaactgctg acgccgctgc gcgatcagtt
     4921 cacccgtgca ccgctggata acgacattgg cgtaagtgaa gcgacccgca ttgaccctaa
     4981 cgcctgggtc gaacgctgga aggcggcggg ccattaccag gccgaagcag cgttgttgca
     5041 gtgcacggca gatacacttg ctgatgcggt gctgattacg accgctcacg cgtggcagca
     5101 tcaggggaaa accttattta tcagccggaa aacctaccgg attgatggta gtggtcaaat
     5161 ggcgattacc gttgatgttg aagtggcgag cgatacaccg catccggcgc ggattggcct
     5221 gaactgccag ctggcgcagg tagcagagcg ggtaaactgg ctcggattag ggccgcaaga
     5281 aaactatccc gaccgcctta ctgccgcctg ttttgaccgc tgggatctgc cattgtcaga
     5341 catgtatacc ccgtacgtct tcccgagcga aaacggtctg cgctgcggga cgcgcgaatt
     5401 gaattatggc ccacaccagt ggcgcggcga cttccagttc aacatcagcc gctacagtca
     5461 acagcaactg atggaaacca gccatcgcca tctgctgcac gcggaagaag gcacatggct
     5521 gaatatcgac ggtttccata tggggattgg tggcgacgac tcctggagcc cgtcagtatc
     5581 ggcggaattc cagctgagcg ccggtcgcta ccattaccag ttggtctggt gtcaaaaata
     5641 aactagtcga ctcacactgg ctcaccttcg ggtgggcctt tctgcgttta tatactagag
     5701 agagaatata aaaagccaga ttattaatcc ggctttttta ttatttaatt cctgttataa
     5761 aaaaaggatc aattttgaac tctctcccaa agttgatccc ttaacgatct aaaacaattc
     5821 atccagtaaa atataatatt ttattttctc ccaatcaggc ttgatcccca gtaagtcaaa
     5881 aaatagctcg acatactgtt cttccccgat atcctccctg atcgaccgga cgcagaaggc
     5941 aatgtcatac cacttgtccg ccctgccgct tctcccaaga tcaataaagc cacttacttt
     6001 gccatctttc acaaagatgt tgctgtctcc caggtcgccg tgggaaaaga caagttcctc
     6061 ttcgggcttt tccgtcttta aaaaatcata cagctcgcgc ggatctttaa atggagtgtc
     6121 ttcttcccag ttttcgcaat ccacatcggc cagatcgtta ttcagtaagt aatccaattc
     6181 ggctaagcgg ctgtctaagc tattcgtata gggacaatcc gatatgtcga tggagtgaaa
     6241 gagcctgatg cactccgcat acagctcgat aatcttttca gggctttgtt catcttcata
     6301 ctcttccgag caaaggacgc catcggcctc actcatgagc agattgctcc agccatcatg
     6361 ccgttcaaag tgcaggacct ttggaacagg cagctttcct tccagccata gcatcatgtc
     6421 cttttcccgt tccacatcat aggtggtccc tttataccgg ctgtccgtca tttttaaata
     6481 taggttttca ttttctccca ccagcttata taccttagca ggagacattc cttccgtatc
     6541 ttttacgcag cggtattttt cgatcagttt tttcaattcc ggtgatattc tcattttagc
     6601 catttattat ttccttcctc ttttctacag tatttaaaga taccccaaga agctaattat
     6661 aacaagacga actccaattc actgttcctt gcattctaaa accttaaata ccagaaaaca
     6721 gctttttcaa agttgttttc aaagttggcg tataacatag tatcgacgga gccgattttg
     6781 aaaccacaat tatgatagaa tttacaagct ataaggttat tgtcctgggt ttcaagcatt
     6841 agtccatgca agtttttatg ctttgcccat tctatagata tattgataag aagactggaa
     6901 tgcccgctcc gccgggatta ttcgaaagcg catcgtccaa gcgagaccgt ctgcattcgg
     6961 aaatctcggc cggttcgcgc cgaggaagcc catctggctg catcccaaat cgacacacaa
     7021 cgcacggagg ccccggcgat cgccggggcc tccgaatcat gcgcatcgtg gaagcgaagc
     7081 tccgcaaaaa accccgcttc agcggggttt tttcgcgtcg cacctccttg tcgtgtatta
     7141 caggtactac tataacacat catctagccc gcccgcgata cgtcctctgc ggtgcagcgt
     7201 ggtcgcggtt ccacgcatcc gccgcccgct gcgcatcgcg cagcacgtca ttaaggctct
     7261 gttgccttgg ctgttccctg cgcatcagat attcgagcgg gcgcgatatt cgacttctaa
     7321 gctcttccac tttgattcgc attcccgcaa ctcctgctct gatgcggctc gcgagtccat
     7381 cgcacttaac aacgatgtta tcgaggattg cgccgcgctc agcttttccc gcagcttccc
     7441 agccccgaac ctcggtagcg atgggttgaa gctctccaat gtctcgcgca actccgtccg
     7501 ctcgttgccg aagacactcc aatcggtctt gctcatcttc gatatcgagc ttcacgtcct
     7561 cgagctggct gtcaagctcg gcaaggtcac cctccttgtc ggcaatctcg gtaacaagct
     7621 ctgccttacg cgtttcggca tcattcagta aagcggcggc ttcttcccta gccgccgcga
     7681 cgattcgcgc cgcttcgtcc ttcgcaggtt caaccacggc cttatcgacc gcacagcgta
     7741 ccttgtcgat atccacggac ttgtccgtgt atacacgctg cgcccgcgtc tcctcgtcaa
     7801 gctcgattgc agggcggtag cccatgcgcc cctctaggta atccccaagc tccttgtgca
     7861 tgctccggta gaagctgcgg gggcacatct gcttgtagtt gaaccgtcca tctaggatgg
     7921 gcgtgaacgg cacgtgcatg tggtcgcggg cgggcgtgcc atccttcaac acctcgtcct
     7981 tgtggacgta cccgcccatc atgttctcga tgccgaactt cttcgacagg taccagtacg
     8041 tgagacggaa gaacctgtcc tcatcgccct tgcgcacatt gtcgggaagc gtcacaacaa
     8101 cgtcagccat aacgaccgca tccttgcgcg tggcacgctt ccctgcggcc ttctgggcct
     8161 cgttcacgcg ctcgatgcgg ctctccaccg tgccccagtt cggctcgcca tcgcgcggct
     8221 ccacgcgccc cacaacgagc ttgccgtcct tgttcgtgta gtggccgacc accaagtcgt
     8281 tcttgatgcg ctcggggtcg atgttgtcgc gctccaggct gctgcggtcg cggcggtagt
     8341 gggcaagcat gtgcccgacc gatggagcct tgtatttcgc gatgtgagcc atggaaacct
     8401 ccgaaccgta cagacttaat cgactaaagt ctagcacgtt agaccttagc tacgctaagg
     8461 acgtggcagg gccagccctg cacccgctcg ggcgttcccc ccgagacccc ccagcccttg
     8521 cccgttcgcg gcttcgctca cggccctcgg gccatcggcg cgagcgccga acggagggac
     8581 ttcgcccctc cgaaccaccc ctcaatctac ctgcggttcg gcacacggca cacggcacta
     8641 ttaggggggc cgtcgtagtg gcccccctgt ggtcatttgt catttgtcat ttgtcacatt
     8701 atttttcgga cacattattt ttcggacgta gttagcgaaa aataatgagc cgcccgattc
     8761 tgcaggtctc atccagtcga gacacgggca aggaaagggt cgatggagca gagcgtccat
     8821 cgacccttaa tcaagtctgg tctattcggt tggccgtcgc gtcaattccg caatccgtga
     8881 acgagtcggc ttagatctgc gcgagcgcag accacgccgc cccgtagtca cgcattgctg
     8941 cattgacccg actactcttt ggcacctgac tgttccacgc acttgcgctt acgttcggcg
     9001 ttcagatagt tcccgcgaca atagcgctcg agaatcgagt tgtaccccac gatgatttcg
     9061 cggtactgct cgggaaactc cctcagcgcc gcagccgtga aatcgttgat ggtctcgaac
     9121 ctctcgcaca tgatgaagcc gaccaactcg aagagcatgt cctgcttgtc cttagtcgag
     9181 agctgttcga gctgaccaat gtcgaagttg tttccctcga tgcgtgcttc gaccggatac
     9241 agctccttgc ccttcttgcg acaggaagcc gtgtcgtgaa tcagatagtc atacgcatgc
     9301 ctgatgttgt tgattggctc ggctgacgaa cagcacttct tgccgtccgc gctcaatcgg
     9361 ttcaagacgt tgatgattgc cttgcgtgtc gtgttgccgc cccagacaac aatgacgtgc
     9421 gcatgcgtct tgcgctgctt gctcttctgg tcatggtcta tgtcgtgaac cgcataagcg
     9481 aaaggcactt gaagaatgtc gtctatctcg tcctgccagt catcgcgcat gttctctggg
     9541 tagcagatgc cctcccagag cttcaccttc gtctcgccag cgtttgccgc catgctata
//
