LOCUS       sequence-375798-       13380 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression vector for an inducible 3xFLAG-KPNA1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13380)
  AUTHORS   Wang L, Paudel BB, McKnight RA, Janes KA
  TITLE     Nucleocytoplasmic transport of active HER2 causes fractional escape 
            from the DCIS-like state.
  JOURNAL   Nat Commun. 2023 Apr 13;14(1):2110. doi: 10.1038/s41467-023-37914-x.
  PUBMED    37055441
REFERENCE   2  (bases 1 to 13380)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-04-13"; volume: "14(1):2110. doi"; pages: " 
            10.1038/s41467-023-37914-x"
COMMENT     Sequence Label: pSLIK 3xFLAG-KPNA1 zeo
FEATURES             Location/Qualifiers
     source          1..13380
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1680..1913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2098..2142
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2440..2557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    2614..2638
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        2670..2984
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    2678..2696
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2714..2732
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2749..2767
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2785..2803
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2821..2839
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2856..2874
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2892..2910
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2957..2981
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             2993..3058
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     protein_bind    complement(4717..4741)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        4791..6002
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     5839..5858
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             join(6061..6678,6679..6684,6685..6720,6721..6723,6724..6759,
                     6760..6762,6763..6798,6799..6807)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    6865..7432
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(7026..7043)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     7253..7272
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     promoter        7454..7501
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7520..7894
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     misc_feature    8068..8656
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8121..8141)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8539..8550)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(8659..8675)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8660..8676)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             9185..9365
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(9391..9408)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    9397..9621
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      9667..10095
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9754..9773)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9964..9985
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(10104..10124)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        10109..10438
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      10289..10424
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     10351..10370
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        10486..10533
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             10552..10926
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    11056..11177
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11093..11112)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11147..11166
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(11226..11242)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11226..11242)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11239..11261)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11250..11266
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11274..11280,11281..11298,11299..11304))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11319..11340
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(11457..11474)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11628..12216)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11708..11727)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12387..13178,13179..13247))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     13010..13029
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13248..13352)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaattc tagacagtta tcaacaagtt tgtacaaaaa agcaggcttt
     2641 aaaggaacca attcagtcga ctggatcccg agtttactcc ctatcagtga tagagaacgt
     2701 atgtcgagtt tactccctat cagtgataga gaacgatgtc gagtttactc cctatcagtg
     2761 atagagaacg tatgtcgagt ttactcccta tcagtgatag agaacgtatg tcgagtttac
     2821 tccctatcag tgatagagaa cgtatgtcga gtttatccct atcagtgata gagaacgtat
     2881 gtcgagttta ctccctatca gtgatagaga acgtatgtcg aggtaggcgt gtacggtggg
     2941 aggcctatat aagcagagct cgtttagtga accgtcagat cgccactaga tggactacaa
     3001 agaccatgac ggtgattata aagatcatga catcgattac aaggatgacg atgacaagac
     3061 tagtatgacc accccaggaa aagagaactt tcgcctgaaa agttacaaga acaaatctct
     3121 gaatcccgat gagatgcgca ggaggaggga ggaagaagga ctgcagttac gaaagcagaa
     3181 aagagaagag cagttattca agcggagaaa tgttgctaca gcagaagaag aaacagaaga
     3241 agaagttatg tcagatggag gctttcatga ggctcagatt aataacatgg agatggcacc
     3301 aggtggtgtc atcacttctg acatgattga aatgatattt tccaaaagcc cagagcaaca
     3361 gctttcagca acacagaaat tcaggaagct gctttcaaaa gaacctaacc ctcctattga
     3421 tgaagttatc agcacaccag gagtagtggc caggtttgtg gagttcctca aacgaaaaga
     3481 gaattgtaca ctgcagtttg aatcagcttg ggtactgaca aatattgctt caggaaattc
     3541 tcttcagacc cgaattgtga ttcaggcagg agctgtgccc atcttcatag agttgctcag
     3601 ctcagagttt gaagatgtcc aggaacaggc agtctgggct cttggcaaca ttgctggaga
     3661 tagtaccatg tgcagggact atgtcttaga ctgcaatatc cttccccctc ttttgcagtt
     3721 attttcaaag caaaaccgcc tgaccatgac ccggaatgca gtatgggctt tgtctaatct
     3781 ctgtagaggg aaaagtccac ctccagaatt tgcaaaggtt tctccatgtc tgaatgtgct
     3841 ttcctggttg ctgtttgtca gtgacactga tgtactggct gatgcctgct gggccctctc
     3901 atatctatca gatggaccca atgataaaat tcaagcggtc atcgatgcgg gagtatgtag
     3961 gagacttgtg gaactgctga tgcataatga ttataaagtg gtttctcctg ctttgcgagc
     4021 tgtgggaaac attgtcacag gggatgatat tcagacacag gtaattctga attgctcagc
     4081 tctgcagagt ttattgcatt tgctgagtag cccaaaggaa tctatcaaaa aggaagcatg
     4141 ttggacgata tctaatatta cagctggaaa tagggcacag atccagactg tgatagatgc
     4201 caacattttc ccagccctca ttagtatttt acaaactgct gaatttcgga caagaaaaga
     4261 agcagcttgg gccatcacaa atgcaacttc tggaggatca gctgaacaga tcaagtacct
     4321 agtagaactg ggttgtatca agccgctctg tgatctcctc acggtcatgg actctaagat
     4381 tgtacaggtt gccctaaatg gcttggaaaa tatcctgagg cttggagaac aggaagccaa
     4441 aaggaatggc actggcatta acccttactg tgctttgatt gaagaagctt atggtctgga
     4501 taaaattgag ttcttacaga gtcatgaaaa ccaggagatc taccaaaagg cctttgatct
     4561 tattgagcat tacttcggga ccgaagatga agacagcagc attgcacccc aggttgacct
     4621 taaccagcag cagtacatct tccaacagtg tgaggctcct atggaaggtt tccagctttg
     4681 acaattggaa gactaatgcg ttttcgagat atctagaccc agctttcttg tacaaagtgg
     4741 ttgataacat ctcgagccat aaagatggtt aattaaccca cccaagatct ggcctccgcg
     4801 ccgggttttg gcgcctcccg cgggcgcccc cctcctcacg gcgagcgctg ccacgtcaga
     4861 cgaagggcgc agcgagcgtc ctgatccttc cgcccggacg ctcaggacag cggcccgctg
     4921 ctcataagac tcggccttag aaccccagta tcagcagaag gacattttag gacgggactt
     4981 gggtgactct agggcactgg ttttctttcc agagagcgga acaggcgagg aaaagtagtc
     5041 ccttctcggc gattctgcgg agggatctcc gtggggcggt gaacgccgat gattatataa
     5101 ggacgcgccg ggtgtggcac agctagttcc gtcgcagccg ggatttgggt cgcggttctt
     5161 gtttgtggat cgctgtgatc gtcacttggt gagtagcggg ctgctgggct ggccggggct
     5221 ttcgtggccg ccgggccgct cggtgggacg gaagcgtgtg gagagaccgc caagggctgt
     5281 agtctgggtc cgcgagcaag gttgccctga actgggggtt ggggggagcg cagcaaaatg
     5341 gcggctgttc ccgagtcttg aatggaagac gcttgtgagg cgggctgtga ggtcgttgaa
     5401 acaaggtggg gggcatggtg ggcggcaaga acccaaggtc ttgaggcctt cgctaatgcg
     5461 ggaaagctct tattcgggtg agatgggctg gggcaccatc tggggaccct gacgtgaagt
     5521 ttgtcactga ctggagaact cggtttgtcg tctgttgcgg gggcggcagt tatggcggtg
     5581 ccgttgggca gtgcacccgt acctttggga gcgcgcgccc tcgtcgtgtc gtgacgtcac
     5641 ccgttctgtt ggcttataat gcagggtggg gccacctgcc ggtaggtgtg cggtaggctt
     5701 ttctccgtcg caggacgcag ggttcgggcc tagggtaggc tctcctgaat cgacaggcgc
     5761 cggacctctg gtgaggggag ggataagtga ggcgtcagtt tctttggtcg gttttatgta
     5821 cctatcttct taagtagctg aagctccggt tttgaactat gcgctcgggg ttggcgagtg
     5881 tgttttgtga agttttttag gcaccttttg aaatgtaatc atttgggtca atatgtaatt
     5941 ttcagtgtta gactagtaaa ttgtccgcta aattctggcc gtttttggct tttttgttag
     6001 acgaagcttg gtaccgagct cggatctcca ccccgtaccg gtcctgcagt cgaattcacc
     6061 atgtctagac tggacaagag caaagtcata aacggagctc tggaattact caatggtgtc
     6121 ggtatcgaag gcctgacgac aaggaaactc gctcaaaagc tgggagttga gcagcctacc
     6181 ctgtactggc acgtgaagaa caagcgggcc ctgctcgatg ccctgccaat cgagatgctg
     6241 gacaggcatc atacccactt ctgccccctg gaaggcgagt catggcaaga ctttctgcgg
     6301 aacaacgcca agtcataccg ctgtgctctc ctctcacatc gcgacggggc taaagtgcat
     6361 ctcggcaccc gcccaacaga gaaacagtac gaaaccctgg aaaatcagct cgcgttcctg
     6421 tgtcagcaag gcttctccct ggagaacgca ctgtacgctc tgtccgccgt gggccacttt
     6481 acactgggct gcgtattgga ggaacaggag catcaagtag caaaagagga aagagagaca
     6541 cctaccaccg attctatgcc cccacttctg agacaagcaa ttgagctgtt cgaccggcag
     6601 ggagccgaac ctgccttcct tttcggcctg gaactaatca tatgtggcct ggagaaacag
     6661 ctaaagtgcg aaagcggcgg gccgaccgac gcccttgacg attttgactt agacatgctc
     6721 ccagccgatg cccttgacga ctttgacctt gatatgctgc ctgctgacgc tcttgacgat
     6781 tttgaccttg acatgctccc cgggtaacta agtaaggatc cgcggccgca ctagaggaat
     6841 tccgcccctc tccccccccc nnyctccctc ccccccccct aacgttactg gccgaagccg
     6901 cttggaataa ggccggtgtg cgtttgtcta tatgttattt tccaccatat tgccgtcttt
     6961 tggcaatgtg agggcccgga aacctggccc tgtcttcttg acgagcattc ctaggggtct
     7021 ttcccctctc gccaaaggaa tgcaaggtct gttgaatgtc gtgaaggaag cagttcctct
     7081 ggaagcttct tgaagacaaa caacgtctgt agcgaccctt tgcaggcagc ggaacccccc
     7141 acctggcgac aggtgcctct gcggccaaaa gccacgtgta taagatacac ctgcaaaggc
     7201 ggcacaaccc cagtgccacg ttgtgagttg gatagttgtg gaaagagtca aatggctctc
     7261 ctcaagcgta ttcaacaagg ggctgaagga tgcccagaag gtaccccatt gtatgggatc
     7321 tgatctgggg cctcggtgca catgctttac atgtgtttag tcgaggttaa aaaaacgtct
     7381 aggccccccg aaccacgggg acgtggtttt cctttgaaaa acacgatgat aagcttaccg
     7441 gtacgcgtga tgtgttgaca attaatcatc ggcatagtat atcggcatag tataatacga
     7501 caaggtgagg aactaaacca tggccaagtt gaccagtgcc gttccggtgc tcaccgcgcg
     7561 cgacgtcgcc ggagcggtcg agttctggac cgaccggctc gggttctccc gggacttcgt
     7621 ggaggacgac ttcgccggtg tggtccggga cgacgtgacc ctgttcatca gcgcggtcca
     7681 ggaccaggtg gtgccggaca acaccctggc ctgggtgtgg gtgcgcggcc tggacgagct
     7741 gtacgccgag tggtcggagg tcgtgtccac gaacttccgg gacgcctccg ggccggccat
     7801 gaccgagatc ggcgagcagc cgtgggggcg ggagttcgcc ctgcgcgacc cggccggcaa
     7861 ctgcgtgcac ttcgtggccg aggagcagga ctgacacatc tgtacaagta aagcggccgc
     7921 gactctagat cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc
     7981 tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gtttagtccc
     8041 tcccaattcg atatcaagct tatcgataat caacctctgg attacaaaat ttgtgaaaga
     8101 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg
     8161 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc
     8221 tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc
     8281 actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt
     8341 tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt
     8401 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg
     8461 aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg
     8521 tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg
     8581 ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt
     8641 tgggccgcct ccccgcatcg ataccgtcga cctcgatcga gacctagaaa aacatggagc
     8701 aatcacaagt agcaatacag cagctaccaa tgctgattgt gcctggctag aagcacaaga
     8761 ggaggaggag gtgggttttc cagtcacacc tcaggtacct ttaagaccaa tgacttacaa
     8821 ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca
     8881 ctcccaacga agacaagata tccttgatct gtggatctac cacacacaag gctacttccc
     8941 tgattggcag aactacacac cagggccagg gatcagatat ccactgacct ttggatggtg
     9001 ctacaagcta gtaccagttg agcaagagaa ggtagaagaa gccaatgaag gagagaacac
     9061 ccgcttgtta caccctgtga gcctgcatgg gatggatgac ccggagagag aagtattaga
     9121 gtggaggttt gacagccgcc tagcatttca tcacatggcc cgagagctgc atccggactg
     9181 tactgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
     9241 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
     9301 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
     9361 tagcagggcc cgtttaaacc cgctgatcag cctcgactgt gccttctagt tgccagccat
     9421 ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc
     9481 tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg
     9541 ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg
     9601 gggatgcggt gggctctatg gcttctgagg cggaaagaac cagctggggc tctagggggt
     9661 atccccacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
     9721 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
     9781 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
     9841 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta
     9901 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
     9961 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg
    10021 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa
    10081 aatttaacgc gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg
    10141 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg
    10201 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
    10261 aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
    10321 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctctgc
    10381 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa
    10441 gctcccggga gcttgtatat ccattttcgg atctgatcag cacgtgttga caattaatca
    10501 tcggcatagt atatcggcat agtataatac gacaaggtga ggaactaaac catggccaag
    10561 ttgaccagtg ccgttccggt gctcaccgcg cgcgacgtcg ccggagcggt cgagttctgg
    10621 accgaccggc tcgggttctc ccgggacttc gtggaggacg acttcgccgg tgtggtccgg
    10681 gacgacgtga ccctgttcat cagcgcggtc caggaccagg tggtgccgga caacaccctg
    10741 gcctgggtgt gggtgcgcgg cctggacgag ctgtacgccg agtggtcgga ggtcgtgtcc
    10801 acgaacttcc gggacgcctc cgggccggcc atgaccgaga tcggcgagca gccgtggggg
    10861 cgggagttcg ccctgcgcga cccggccggc aactgcgtgc acttcgtggc cgaggagcag
    10921 gactgacacg tgctacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc
    10981 ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag
    11041 ttcttcgccc accccaactt gtttattgca gcttataatg gttacaaata aagcaatagc
    11101 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
    11161 ctcatcaatg tatcttatca tgtctgtata ccgtcgacct ctagctagag cttggcgtaa
    11221 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
    11281 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
    11341 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
    11401 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
    11461 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
    11521 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
    11581 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
    11641 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
    11701 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
    11761 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
    11821 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
    11881 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
    11941 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
    12001 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
    12061 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
    12121 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
    12181 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
    12241 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
    12301 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
    12361 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
    12421 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
    12481 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
    12541 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
    12601 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
    12661 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
    12721 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
    12781 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
    12841 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
    12901 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
    12961 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
    13021 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
    13081 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
    13141 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
    13201 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
    13261 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
    13321 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
//
