LOCUS       sequence-375803-       13345 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression vector for an inducible 3xFLAG-KPNA2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13345)
  AUTHORS   Wang L, Paudel BB, McKnight RA, Janes KA
  TITLE     Nucleocytoplasmic transport of active HER2 causes fractional escape 
            from the DCIS-like state.
  JOURNAL   Nat Commun. 2023 Apr 13;14(1):2110. doi: 10.1038/s41467-023-37914-x.
  PUBMED    37055441
REFERENCE   2  (bases 1 to 13345)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-04-13"; volume: "14(1):2110. doi"; pages: " 
            10.1038/s41467-023-37914-x"
COMMENT     Sequence Label: pSLIK 3xFLAG-KPNA2 zeo
FEATURES             Location/Qualifiers
     source          1..13345
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1680..1913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2098..2142
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2440..2557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    2614..2638
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        2670..2984
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    2678..2696
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2714..2732
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2749..2767
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2785..2803
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2821..2839
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2856..2874
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2892..2910
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2957..2981
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             2993..3058
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     protein_bind    complement(4696..4720)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        4770..5981
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     5818..5837
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             join(6040..6657,6658..6663,6664..6699,6700..6702,6703..6738,
                     6739..6741,6742..6777,6778..6786)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    6824..7397
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6991..7008)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     7218..7237
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     promoter        7419..7466
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7485..7859
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     misc_feature    8033..8621
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8086..8106)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8504..8515)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(8624..8640)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8625..8641)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             9150..9330
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(9356..9373)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    9362..9586
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      9632..10060
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9719..9738)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9929..9950
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(10069..10089)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        10074..10403
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      10254..10389
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     10316..10335
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        10451..10498
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             10517..10891
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    11021..11142
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11058..11077)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11112..11131
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(11191..11207)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11191..11207)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11204..11226)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11215..11231
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11239..11245,11246..11263,11264..11269))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11284..11305
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(11422..11439)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11593..12181)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11673..11692)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12352..13143,13144..13212))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12975..12994
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13213..13317)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaattc tagacagtta tcaacaagtt tgtacaaaaa agcaggcttt
     2641 aaaggaacca attcagtcga ctggatcccg agtttactcc ctatcagtga tagagaacgt
     2701 atgtcgagtt tactccctat cagtgataga gaacgatgtc gagtttactc cctatcagtg
     2761 atagagaacg tatgtcgagt ttactcccta tcagtgatag agaacgtatg tcgagtttac
     2821 tccctatcag tgatagagaa cgtatgtcga gtttatccct atcagtgata gagaacgtat
     2881 gtcgagttta ctccctatca gtgatagaga acgtatgtcg aggtaggcgt gtacggtggg
     2941 aggcctatat aagcagagct cgtttagtga accgtcagat cgccactaga tggactacaa
     3001 agaccatgac ggtgattata aagatcatga catcgattac aaggatgacg atgacaagac
     3061 tagtatgtcc accaacgaga atgctaatac accagctgcc cgtcttcaca gattcaagaa
     3121 caagggaaaa gacagtacag aaatgaggcg tcgcagaata gaggtcaatg tggagctgag
     3181 gaaagctaag aaggatgacc agatgctgaa gaggagaaat gtaagctcat ttcctgatga
     3241 tgctacttct ccgctgcagg aaaaccgcaa caaccagggc actgtaaatt ggtctgttga
     3301 tgacattgtc aaaggcataa atagcagcaa tgtggaaaat cagctccaag ctactcaagc
     3361 tgccaggaaa ctactttcca gagaaaaaca gccccccata gacaacataa tccgggctgg
     3421 tttgattccg aaatttgtgt ccttcttggg cagaactgat tgtagtccca ttcagtttga
     3481 atctgcttgg gcactcacta acattgcttc tgggacatca gaacaaacca aggctgtggt
     3541 agatggaggt gccatcccag cattcatttc tctgttggca tctccccatg ctcacatcag
     3601 tgaacaagct gtctgggctc taggaaacat tgcaggtgat ggctcagtgt tccgagactt
     3661 ggttattaag tacggtgcag ttgacccact gttggctctc cttgcagttc ctgagatgtc
     3721 atctttagca tgtggctact tacgtaatct tacctggaca ctttctaatc tttgccgcaa
     3781 caagaatcct gcacccccga tagatgctgt tgagcagatt cttcctacct tagttcggct
     3841 cctgcatcat gatgatccag aagtgttagc agatacctgc tgggctattt cctaccttac
     3901 tgatggtcca aatgaacgaa ttggcatggt ggtgaaaaca ggagttgtgc cccaacttgt
     3961 gaagcttcta ggagcttctg aattgccaat tgtgactcct gccctaagag ccatagggaa
     4021 tattgtcact ggtacagatg aacagactca ggttgtgatt gatgcaggag cactcgccgt
     4081 ctttcccagc ctgctcacca accccaaaac taacattcag aaggaagcta cgtggacaat
     4141 gtcaaacatc acagccggcc gccaggacca gatacagcaa gttgtgaatc atggattagt
     4201 cccattcctt gtcagtgttc tctctaaggc agattttaag acacaaaagg aagctgtgtg
     4261 ggccgtgacc aactatacca gtggtggaac agttgaacag attgtgtacc ttgttcactg
     4321 tggcataata gaaccgttga tgaacctctt aactgcaaaa gataccaaga ttattctggt
     4381 tatcctggat gccatttcaa atatctttca ggctgctgag aaactaggtg aaactgagaa
     4441 acttagtata atgattgaag aatgtggagg cttagacaaa attgaagctc tacaaaacca
     4501 tgaaaatgag tctgtgtata aggcttcgtt aagcttaatt gagaagtatt tctctgtaga
     4561 ggaagaggaa gatcaaaacg ttgtaccaga aactacctct gaaggctaca ctttccaagt
     4621 tcaggatggg gctcctggga cctttaactt ttgagcatgc caattggaag actaatgcgt
     4681 tttcgagata tctagaccca gctttcttgt acaaagtggt tgataacatc tcgagccata
     4741 aagatggtta attaacccac ccaagatctg gcctccgcgc cgggttttgg cgcctcccgc
     4801 gggcgccccc ctcctcacgg cgagcgctgc cacgtcagac gaagggcgca gcgagcgtcc
     4861 tgatccttcc gcccggacgc tcaggacagc ggcccgctgc tcataagact cggccttaga
     4921 accccagtat cagcagaagg acattttagg acgggacttg ggtgactcta gggcactggt
     4981 tttctttcca gagagcggaa caggcgagga aaagtagtcc cttctcggcg attctgcgga
     5041 gggatctccg tggggcggtg aacgccgatg attatataag gacgcgccgg gtgtggcaca
     5101 gctagttccg tcgcagccgg gatttgggtc gcggttcttg tttgtggatc gctgtgatcg
     5161 tcacttggtg agtagcgggc tgctgggctg gccggggctt tcgtggccgc cgggccgctc
     5221 ggtgggacgg aagcgtgtgg agagaccgcc aagggctgta gtctgggtcc gcgagcaagg
     5281 ttgccctgaa ctgggggttg gggggagcgc agcaaaatgg cggctgttcc cgagtcttga
     5341 atggaagacg cttgtgaggc gggctgtgag gtcgttgaaa caaggtgggg ggcatggtgg
     5401 gcggcaagaa cccaaggtct tgaggccttc gctaatgcgg gaaagctctt attcgggtga
     5461 gatgggctgg ggcaccatct ggggaccctg acgtgaagtt tgtcactgac tggagaactc
     5521 ggtttgtcgt ctgttgcggg ggcggcagtt atggcggtgc cgttgggcag tgcacccgta
     5581 cctttgggag cgcgcgccct cgtcgtgtcg tgacgtcacc cgttctgttg gcttataatg
     5641 cagggtgggg ccacctgccg gtaggtgtgc ggtaggcttt tctccgtcgc aggacgcagg
     5701 gttcgggcct agggtaggct ctcctgaatc gacaggcgcc ggacctctgg tgaggggagg
     5761 gataagtgag gcgtcagttt ctttggtcgg ttttatgtac ctatcttctt aagtagctga
     5821 agctccggtt ttgaactatg cgctcggggt tggcgagtgt gttttgtgaa gttttttagg
     5881 caccttttga aatgtaatca tttgggtcaa tatgtaattt tcagtgttag actagtaaat
     5941 tgtccgctaa attctggccg tttttggctt ttttgttaga cgaagcttgg taccgagctc
     6001 ggatctccac cccgtaccgg tcctgcagtc gaattcacca tgtctagact ggacaagagc
     6061 aaagtcataa acggagctct ggaattactc aatggtgtcg gtatcgaagg cctgacgaca
     6121 aggaaactcg ctcaaaagct gggagttgag cagcctaccc tgtactggca cgtgaagaac
     6181 aagcgggccc tgctcgatgc cctgccaatc gagatgctgg acaggcatca tacccacttc
     6241 tgccccctgg aaggcgagtc atggcaagac tttctgcgga acaacgccaa gtcataccgc
     6301 tgtgctctcc tctcacatcg cgacggggct aaagtgcatc tcggcacccg cccaacagag
     6361 aaacagtacg aaaccctgga aaatcagctc gcgttcctgt gtcagcaagg cttctccctg
     6421 gagaacgcac tgtacgctct gtccgccgtg ggccacttta cactgggctg cgtattggag
     6481 gaacaggagc atcaagtagc aaaagaggaa agagagacac ctaccaccga ttctatgccc
     6541 ccacttctga gacaagcaat tgagctgttc gaccggcagg gagccgaacc tgccttcctt
     6601 ttcggcctgg aactaatcat atgtggcctg gagaaacagc taaagtgcga aagcggcggg
     6661 ccgaccgacg cccttgacga ttttgactta gacatgctcc cagccgatgc ccttgacgac
     6721 tttgaccttg atatgctgcc tgctgacgct cttgacgatt ttgaccttga catgctcccc
     6781 gggtaactaa gtaaggatcc gcggccgcac tagaggaatt ccgcccctct cccycccccc
     6841 cccctaacgt tactggccga agccgcttgg aataaggccg gtgtgcgttt gtctatatgt
     6901 tattttccac catattgccg tcttttggca atgtgagggc ccggaaacct ggccctgtct
     6961 tcttgacgag cattcctagg ggtctttccc ctctcgccaa aggaatgcaa ggtctgttga
     7021 atgtcgtgaa ggaagcagtt cctctggaag cttcttgaag acaaacaacg tctgtagcga
     7081 ccctttgcag gcagcggaac cccccacctg gcgacaggtg cctctgcggc caaaagccac
     7141 gtgtataaga tacacctgca aaggcggcac aaccccagtg ccacgttgtg agttggatag
     7201 ttgtggaaag agtcaaatgg ctctcctcaa gcgtattcaa caaggggctg aaggatgccc
     7261 agaaggtacc ccattgtatg ggatctgatc tggggcctcg gtgcacatgc tttacatgtg
     7321 tttagtcgag gttaaaaaaa cgtctaggcc ccccgaacca cggggacgtg gttttccttt
     7381 gaaaaacacg atgataagct taccggtacg cgtgatgtgt tgacaattaa tcatcggcat
     7441 agtatatcgg catagtataa tacgacaagg tgaggaacta aaccatggcc aagttgacca
     7501 gtgccgttcc ggtgctcacc gcgcgcgacg tcgccggagc ggtcgagttc tggaccgacc
     7561 ggctcgggtt ctcccgggac ttcgtggagg acgacttcgc cggtgtggtc cgggacgacg
     7621 tgaccctgtt catcagcgcg gtccaggacc aggtggtgcc ggacaacacc ctggcctggg
     7681 tgtgggtgcg cggcctggac gagctgtacg ccgagtggtc ggaggtcgtg tccacgaact
     7741 tccgggacgc ctccgggccg gccatgaccg agatcggcga gcagccgtgg gggcgggagt
     7801 tcgccctgcg cgacccggcc ggcaactgcg tgcacttcgt ggccgaggag caggactgac
     7861 acatctgtac aagtaaagcg gccgcgactc tagatcataa tcagccatac cacatttgta
     7921 gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg
     7981 aatgcaattg ttgttgttta gtccctccca attcgatatc aagcttatcg ataatcaacc
     8041 tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac
     8101 gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt
     8161 cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt
     8221 tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg
     8281 cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac
     8341 ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac
     8401 tgacaattcc gtggtgttgt cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt
     8461 tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc
     8521 ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg
     8581 ccctcagacg agtcggatct ccctttgggc cgcctccccg catcgatacc gtcgacctcg
     8641 atcgagacct agaaaaacat ggagcaatca caagtagcaa tacagcagct accaatgctg
     8701 attgtgcctg gctagaagca caagaggagg aggaggtggg ttttccagtc acacctcagg
     8761 tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa
     8821 aggggggact ggaagggcta attcactccc aacgaagaca agatatcctt gatctgtgga
     8881 tctaccacac acaaggctac ttccctgatt ggcagaacta cacaccaggg ccagggatca
     8941 gatatccact gacctttgga tggtgctaca agctagtacc agttgagcaa gagaaggtag
     9001 aagaagccaa tgaaggagag aacacccgct tgttacaccc tgtgagcctg catgggatgg
     9061 atgacccgga gagagaagta ttagagtgga ggtttgacag ccgcctagca tttcatcaca
     9121 tggcccgaga gctgcatccg gactgtactg ggtctctctg gttagaccag atctgagcct
     9181 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
     9241 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
     9301 ccttttagtc agtgtggaaa atctctagca gggcccgttt aaacccgctg atcagcctcg
     9361 actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc
     9421 ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt
     9481 ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat
     9541 tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa
     9601 agaaccagct ggggctctag ggggtatccc cacgcgccct gtagcggcgc attaagcgcg
     9661 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
     9721 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
     9781 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
     9841 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
     9901 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
     9961 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg
    10021 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc
    10081 agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
    10141 tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
    10201 aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc
    10261 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt
    10321 atgcagaggc cgaggccgcc tctgcctctg agctattcca gaagtagtga ggaggctttt
    10381 ttggaggcct aggcttttgc aaaaagctcc cgggagcttg tatatccatt ttcggatctg
    10441 atcagcacgt gttgacaatt aatcatcggc atagtatatc ggcatagtat aatacgacaa
    10501 ggtgaggaac taaaccatgg ccaagttgac cagtgccgtt ccggtgctca ccgcgcgcga
    10561 cgtcgccgga gcggtcgagt tctggaccga ccggctcggg ttctcccggg acttcgtgga
    10621 ggacgacttc gccggtgtgg tccgggacga cgtgaccctg ttcatcagcg cggtccagga
    10681 ccaggtggtg ccggacaaca ccctggcctg ggtgtgggtg cgcggcctgg acgagctgta
    10741 cgccgagtgg tcggaggtcg tgtccacgaa cttccgggac gcctccgggc cggccatgac
    10801 cgagatcggc gagcagccgt gggggcggga gttcgccctg cgcgacccgg ccggcaactg
    10861 cgtgcacttc gtggccgagg agcaggactg acacgtgcta cgagatttcg attccaccgc
    10921 cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct
    10981 ccagcgcggg gatctcatgc tggagttctt cgcccacccc aacttgttta ttgcagctta
    11041 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
    11101 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct gtataccgtc
    11161 gacctctagc tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
    11221 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
    11281 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
    11341 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
    11401 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
    11461 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
    11521 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
    11581 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
    11641 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
    11701 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
    11761 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
    11821 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
    11881 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
    11941 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
    12001 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
    12061 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
    12121 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
    12181 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
    12241 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
    12301 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
    12361 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
    12421 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
    12481 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
    12541 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
    12601 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
    12661 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
    12721 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
    12781 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
    12841 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
    12901 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
    12961 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
    13021 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
    13081 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
    13141 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
    13201 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
    13261 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
    13321 atttccccga aaagtgccac ctgac
//
