LOCUS       sequence-375804-       15316 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression vector for an inducible NPIPB11-FLAG.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15316)
  AUTHORS   Wang L, Paudel BB, McKnight RA, Janes KA
  TITLE     Nucleocytoplasmic transport of active HER2 causes fractional escape 
            from the DCIS-like state.
  JOURNAL   Nat Commun. 2023 Apr 13;14(1):2110. doi: 10.1038/s41467-023-37914-x.
  PUBMED    37055441
REFERENCE   2  (bases 1 to 15316)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-04-13"; volume: "14(1):2110. doi"; pages: " 
            10.1038/s41467-023-37914-x"
COMMENT     Sequence Label: pSLIK NPIPB11-FLAG zeo
FEATURES             Location/Qualifiers
     source          1..15316
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1680..1913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2098..2142
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2440..2557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    2614..2638
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        2670..2984
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    2678..2696
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2714..2732
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2749..2767
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2785..2803
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2821..2839
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2856..2874
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2892..2910
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2957..2981
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      3009..3018
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6381..6404
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     polyA_signal    6430..6541
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    complement(6667..6691)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        6741..7952
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     7789..7808
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             join(8011..8628,8629..8634,8635..8670,8671..8673,8674..8709,
                     8710..8712,8713..8748,8749..8757)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    8795..9368
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(8962..8979)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     9189..9208
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     promoter        9390..9437
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             9456..9830
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     misc_feature    10004..10592
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(10057..10077)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(10475..10486)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(10595..10611)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10596..10612)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             11121..11301
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     polyA_signal    11333..11557
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      11603..12031
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(11690..11709)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     11900..11921
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(12040..12060)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        12045..12374
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      12225..12360
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     12287..12306
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        12422..12469
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             12488..12862
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    12992..13113
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(13029..13048)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     13083..13102
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(13162..13178)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(13162..13178)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(13175..13197)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    13186..13202
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(13210..13216,13217..13234,13235..13240))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    13255..13276
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(13393..13410)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(13564..14152)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(13644..13663)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(14323..15114,15115..15183))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     14946..14965
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(15184..15288)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaattc tagacagtta tcaacaagtt tgtacaaaaa agcaggcttt
     2641 aaaggaacca attcagtcga ctggatcccg agtttactcc ctatcagtga tagagaacgt
     2701 atgtcgagtt tactccctat cagtgataga gaacgatgtc gagtttactc cctatcagtg
     2761 atagagaacg tatgtcgagt ttactcccta tcagtgatag agaacgtatg tcgagtttac
     2821 tccctatcag tgatagagaa cgtatgtcga gtttatccct atcagtgata gagaacgtat
     2881 gtcgagttta ctccctatca gtgatagaga acgtatgtcg aggtaggcgt gtacggtggg
     2941 aggcctatat aagcagagct cgtttagtga accgtcagat cgccactagt ggtaccgagc
     3001 tcggatccgc caccatggtg aagctctcta ttgtcctgac cccacagttc ctgtcccatg
     3061 accagggcca gctcaccaag gagctgcagc agcacgtaaa gtcagtgaca tgcccatgcg
     3121 agtacctgag gaaggttatc aatactctgg ctgaccatca tcatcgtggg actgactttg
     3181 gtggaagtcc ttggttacat attattattg cgtttccgac aagttataaa gttgtcatta
     3241 ccctctggat agtttacctt tgggtgtctc tcctgaagac tatcttctgg tctcgaaatg
     3301 gacatgatgg atccacggat gtacagcaga gagcctggag gtccaaccgc cgtagacagg
     3361 aagggctgag gtccatttgt atgcacacaa agaaaagagt ttcttccttt cgaggaaata
     3421 aaattggcct gaaagacgtc attactctac ggaggcatgt ggaaacaaaa gttagagcta
     3481 aaatccgtaa gaggaaggtg acaacgaaaa tcaaccgtca tgacaaaatc aatggaaaga
     3541 ggaagaccgc cagaaaacag aaaatgtttc aacgtgcgca agagttgcgg cggcgggcag
     3601 aggactacca caaatgcaaa atcccccctt ctgcaagaaa ggctctttgc aactgggtca
     3661 gaatggcggc agcggagcat cgtcattctt caggattgcc ctactggccc tacctcacag
     3721 ctgaaacttt aaaaaacagg atgggccacc agccacctcc tccaactcaa caacattgta
     3781 taactgataa ctccctgagc ctcaagacac ctctcgagtg tctgctcact ccccttccac
     3841 cctcagcgga tgataatctc aagacacctc ccgagtgtct gctcactccc cttccaccct
     3901 cagcggatga taatctcaag acacctcccg agtgtctgct cactcccctt ccaccctcag
     3961 ctccaccctc agctccaccc tcagcggatg ataatctcaa gacacgtgcc gagtgtctgc
     4021 tccatcccct tccaccctca gcggatgata atctcaagac accttccgag cgtcagctca
     4081 ctccccttcc accctcagct ccaccctcag cagatgataa tatcaagaca actgccgagc
     4141 gtctgcgggg gccgcttcca ccctcagcgg atgataatct caagacacct tctgagcgtc
     4201 agctcactcc ccttccaccc tcagctccac cctcagcaga tgataatatc aagacacctg
     4261 ccgagcatct gcgggggccg cttccaccct cagcggatga taatctcaag acaccttctg
     4321 agcgtcagct cactcccctt ccaccctcag ctccaccctc agcagatgat aatatcaaga
     4381 cacctgccga gcgtctgcgg gggccgcttc caccctcagc cgatgataat ctcaagacac
     4441 cttctgagcg tcagctcact ccccttccac cctcagctcc accctcagca gatgataata
     4501 tcaagacacc tgccgagcat ctgcgggggc cgcttccacc ctcagcggat gataatctca
     4561 agacaccttc tgagcgtcag ctcactcccc ttccaccctc agctccaccc tcagcagatg
     4621 ataatatcaa gacaactgcc gagcatctgc gggggccgct tccaccctca gcggatgata
     4681 atctcaagac accttccgag cgtcagctca ctccccttcc accctcagct ccaccctcag
     4741 cagatgataa tatcaagaca cctgccgagc atctgcagtt ccgcttccac cctcagcgga
     4801 tgataatctc aagagacctt ccgagcgtca gctcactccc cttccaccct cagctccacc
     4861 ctcagcagat gataatatca agatacctgc tgagcatctg cgggttccgc ttccaccgtc
     4921 agcggatgat aatctcaaga caccttccga gcgtcagctc actccccttc caccctcagc
     4981 tccaccctca gcagatgata atatcaagac acctgccgag cgtctgcggg ggccgcttcc
     5041 accctcagcc gatgataatc tcaagacacc ttccgagcgt cagctcactc cccttccacc
     5101 ctcagctcca ccctcagcag atgataatat caagacacct gccgagcgtc tgcgggggcc
     5161 gcttccaccc tcagccgatg ataatctcaa gacaccttcc gagcgtcagc tcactcccct
     5221 tccaccctca gctccaccct cagcagatga taatatcaag acacctgccg agcgtctgcg
     5281 ggggccgctt ccaccctcag ccgatgataa tctcaagaca ccttccgagc gtcagctcac
     5341 tccccttcca ccctcagctc caccctcagc agatgataat atcaagacac ctgccgagcg
     5401 tctgcggggg ccgcttccac cctcagccga tgataatctc aagacacctt ccgagcgtca
     5461 gctcactccc cttccaccct cagctccacc ctcagcagat gataatatca agacacctgc
     5521 cgagcgtctg cgggggccgc ttccaccctc agccgatgat aatctcaaga caccttccga
     5581 gcgtcagctc actccccttc caccctcagc tccaccctca gcagatgata atatcaagac
     5641 acctgccgag cgtctgcggg gagcgtctgt ggcgtctgcg ggggccgctt ccaccctcag
     5701 ccgatgataa tctcaagaca ccttccaaac gtcagctcac tccccttcca ccctcagctc
     5761 caccctcagc agatgataat atcaagacac ctgccgagcg tctgcggggg ccgcttccac
     5821 cctcagccga tgataatctc aagacacctt ccgagcgtca gctcactccc cttccaccct
     5881 cagctccacc ctcagcagat gataatatca agacacctgc cgagcgtctg cgggggccgc
     5941 ttccaccctc agcggatgat aatctcaaga caccttccga gcgtcagctc actccccttc
     6001 caccctcagc tccaacctca gcagatgata atatcaagac acctgccgag cgtctgcggg
     6061 ggccgcttcc accctcagcc gatgataatc tcaagacacc tcccttagct actcaggagg
     6121 ctgaggcaga aaaaccacgc aaacccaaga ggcagagggc ggctgagatg gaaccacctc
     6181 ccgaacccaa gaggcggagg gtcggtgacg tggaaccgtc acgcaaaccc aagaggcgga
     6241 gggccgctga cgtggaacca tcatcacccg aacccaagag gcggagggtc ggtgatgtgg
     6301 aaccgtcacg caaacccaag aggcggaggg ccgctgacgt ggaaccatca tcacccgaac
     6361 ccaagaggcg gaggttgagc gattacaagg atgacgacga taagtgataa acccgctgat
     6421 cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt
     6481 ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat
     6541 cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg
     6601 gggaggattg ggaagacaat agcaggcatg ccaattggaa gactaatgcg ttttcgagat
     6661 atctagaccc agctttcttg tacaaagtgg ttgataacat ctcgagccat aaagatggtt
     6721 aattaaccca cccaagatct ggcctccgcg ccgggttttg gcgcctcccg cgggcgcccc
     6781 cctcctcacg gcgagcgctg ccacgtcaga cgaagggcgc agcgagcgtc ctgatccttc
     6841 cgcccggacg ctcaggacag cggcccgctg ctcataagac tcggccttag aaccccagta
     6901 tcagcagaag gacattttag gacgggactt gggtgactct agggcactgg ttttctttcc
     6961 agagagcgga acaggcgagg aaaagtagtc ccttctcggc gattctgcgg agggatctcc
     7021 gtggggcggt gaacgccgat gattatataa ggacgcgccg ggtgtggcac agctagttcc
     7081 gtcgcagccg ggatttgggt cgcggttctt gtttgtggat cgctgtgatc gtcacttggt
     7141 gagtagcggg ctgctgggct ggccggggct ttcgtggccg ccgggccgct cggtgggacg
     7201 gaagcgtgtg gagagaccgc caagggctgt agtctgggtc cgcgagcaag gttgccctga
     7261 actgggggtt ggggggagcg cagcaaaatg gcggctgttc ccgagtcttg aatggaagac
     7321 gcttgtgagg cgggctgtga ggtcgttgaa acaaggtggg gggcatggtg ggcggcaaga
     7381 acccaaggtc ttgaggcctt cgctaatgcg ggaaagctct tattcgggtg agatgggctg
     7441 gggcaccatc tggggaccct gacgtgaagt ttgtcactga ctggagaact cggtttgtcg
     7501 tctgttgcgg gggcggcagt tatggcggtg ccgttgggca gtgcacccgt acctttggga
     7561 gcgcgcgccc tcgtcgtgtc gtgacgtcac ccgttctgtt ggcttataat gcagggtggg
     7621 gccacctgcc ggtaggtgtg cggtaggctt ttctccgtcg caggacgcag ggttcgggcc
     7681 tagggtaggc tctcctgaat cgacaggcgc cggacctctg gtgaggagag ggataagtga
     7741 ggcgtcagtt tctttggtcg gttttatgta cctatcttct taagtagctg aagctccggt
     7801 tttgaactat gcgctcgggg ttggcgagtg tgttttgtga agttttttag gcaccttttg
     7861 aaatgtaatc atttgggtca atatgtaatt ttcagtgtta gactagtaaa ttgtccgcta
     7921 aattctggcc gtttttggct tttttgttag acgaagcttg gtaccgagct cggatctcca
     7981 ccccgtaccg gtcctgcagt cgaattcacc atgtctagac tggacaagag caaagtcata
     8041 aacggagctc tggaattact caatggtgtc ggtatcgaag gcctgacgac aaggaaactc
     8101 gctcaaaagc tgggagttga gcagcctacc ctgtactggc acgtgaagaa caagcgggcc
     8161 ctgctcgatg ccctgccaat cgagatgctg gacaggcatc atacccactt ctgccccctg
     8221 gaaggcgagt catggcaaga ctttctgcgg aacaacgcca agtcataccg ctgtgctctc
     8281 ctctcacatc gcgacggggc taaagtgcat ctcggcaccc gcccaacaga gaaacagtac
     8341 gaaaccctgg aaaatcagct cgcgttcctg tgtcagcaag gcttctccct ggagaacgca
     8401 ctgtacgctc tgtccgccgt gggccacttt acactgggct gcgtattgga ggaacaggag
     8461 catcaagtag caaaagagga aagagagaca cctaccaccg attctatgcc cccacttctg
     8521 agacaagcaa ttgagctgtt cgaccggcag ggagccgaac ctgccttcct tttcggcctg
     8581 gaactaatca tatgtggcct ggagaaacag ctaaagtgcg aaagcggcgg gccgaccgac
     8641 gcccttgacg attttgactt agacatgctc ccagccgatg cccttgacga ctttgacctt
     8701 gatatgctgc ctgctgacgc tcttgacgat tttgaccttg acatgctccc cgggtaacta
     8761 agtaaggatc cgcggccgca ctagaggaat tccgcccctc tcccyccccc ccccctaacg
     8821 ttactggccg aagccgcttg gaataaggcc ggtgtgcgtt tgtctatatg ttattttcca
     8881 ccatattgcc gtcttttggc aatgtgaggg cccggaaacc tggccctgtc ttcttgacga
     8941 gcattcctag gggtctttcc cctctcgcca aaggaatgca aggtctgttg aatgtcgtga
     9001 aggaagcagt tcctctggaa gcttcttgaa gacaaacaac gtctgtagcg accctttgca
     9061 ggcagcggaa ccccccacct ggcgacaggt gcctctgcgg ccaaaagcca cgtgtataag
     9121 atacacctgc aaaggcggca caaccccagt gccacgttgt gagttggata gttgtggaaa
     9181 gagtcaaatg gctctcctca agcgtattca acaaggggct gaaggatgcc cagaaggtac
     9241 cccattgtat gggatctgat ctggggcctc ggtgcacatg ctttacatgt gtttagtcga
     9301 ggttaaaaaa acgtctaggc cccccgaacc acggggacgt ggttttcctt tgaaaaacac
     9361 gatgataagc ttaccggtac gcgtgatgtg ttgacaatta atcatcggca tagtatatcg
     9421 gcatagtata atacgacaag gtgaggaact aaaccatggc caagttgacc agtgccgttc
     9481 cggtgctcac cgcgcgcgac gtcgccggag cggtcgagtt ctggaccgac cggctcgggt
     9541 tctcccggga cttcgtggag gacgacttcg ccggtgtggt ccgggacgac gtgaccctgt
     9601 tcatcagcgc ggtccaggac caggtggtgc cggacaacac cctggcctgg gtgtgggtgc
     9661 gcggcctgga cgagctgtac gccgagtggt cggaggtcgt gtccacgaac ttccgggacg
     9721 cctccgggcc ggccatgacc gagatcggcg agcagccgtg ggggcgggag ttcgccctgc
     9781 gcgacccggc cggcaactgc gtgcacttcg tggccgagga gcaggactga cacatctgta
     9841 caagtaaagc ggccgcgact ctagatcata atcagccata ccacatttgt agaggtttta
     9901 cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat gaatgcaatt
     9961 gttgttgttt agtccctccc aattcgatat caagcttatc gataatcaac ctctggatta
    10021 caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg
    10081 atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc
    10141 ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca
    10201 acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac
    10261 cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact
    10321 catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc
    10381 cgtggtgttg tcggggaaat catcgtcctt tccttggctg ctcgcctgtg ttgccacctg
    10441 gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc
    10501 ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac
    10561 gagtcggatc tccctttggg ccgcctcccc gcatcgatac cgtcgacctc gatcgagacc
    10621 tagaaaaaca tggagcaatc acaagtagca atacagcagc taccaatgct gattgtgcct
    10681 ggctagaagc acaagaggag gaggaggtgg gttttccagt cacacctcag gtacctttaa
    10741 gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac
    10801 tggaagggct aattcactcc caacgaagac aagatatcct tgatctgtgg atctaccaca
    10861 cacaaggcta cttccctgat tggcagaact acacaccagg gccagggatc agatatccac
    10921 tgacctttgg atggtgctac aagctagtac cagttgagca agagaaggta gaagaagcca
    10981 atgaaggaga gaacacccgc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
    11041 agagagaagt attagagtgg aggtttgaca gccgcctagc atttcatcac atggcccgag
    11101 agctgcatcc ggactgtact gggtctctct ggttagacca gatctgagcc tgggagctct
    11161 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag
    11221 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt
    11281 cagtgtggaa aatctctagc agggcccgtt taaacccgct gatcagcctc gactgtgcct
    11341 tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt
    11401 gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg
    11461 tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac
    11521 aatagcaggc atgctgggga tgcggtgggc tctatggctt ctgaggcgga aagaaccagc
    11581 tggggctcta gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
    11641 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct
    11701 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg
    11761 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag
    11821 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg
    11881 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc
    11941 tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat
    12001 gagctgattt aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt cagttagggt
    12061 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
    12121 cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
    12181 atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc
    12241 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg
    12301 ccgaggccgc ctctgcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc
    12361 taggcttttg caaaaagctc ccgggagctt gtatatccat tttcggatct gatcagcacg
    12421 tgttgacaat taatcatcgg catagtatat cggcatagta taatacgaca aggtgaggaa
    12481 ctaaaccatg gccaagttga ccagtgccgt tccggtgctc accgcgcgcg acgtcgccgg
    12541 agcggtcgag ttctggaccg accggctcgg gttctcccgg gacttcgtgg aggacgactt
    12601 cgccggtgtg gtccgggacg acgtgaccct gttcatcagc gcggtccagg accaggtggt
    12661 gccggacaac accctggcct gggtgtgggt gcgcggcctg gacgagctgt acgccgagtg
    12721 gtcggaggtc gtgtccacga acttccggga cgcctccggg ccggccatga ccgagatcgg
    12781 cgagcagccg tgggggcggg agttcgccct gcgcgacccg gccggcaact gcgtgcactt
    12841 cgtggccgag gagcaggact gacacgtgct acgagatttc gattccaccg ccgccttcta
    12901 tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg
    12961 ggatctcatg ctggagttct tcgcccaccc caacttgttt attgcagctt ataatggtta
    13021 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
    13081 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tgtataccgt cgacctctag
    13141 ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac
    13201 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
    13261 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
    13321 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
    13381 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
    13441 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
    13501 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
    13561 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
    13621 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
    13681 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
    13741 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
    13801 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
    13861 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
    13921 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
    13981 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
    14041 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
    14101 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
    14161 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
    14221 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
    14281 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
    14341 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
    14401 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
    14461 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
    14521 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
    14581 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
    14641 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
    14701 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
    14761 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
    14821 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
    14881 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
    14941 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
    15001 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
    15061 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
    15121 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
    15181 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
    15241 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
    15301 aaaagtgcca cctgac
//
