LOCUS       sequence-375842-       14417 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression vector for an inducible 3xFLAG-KIF20A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14417)
  AUTHORS   Wang L, Paudel BB, McKnight RA, Janes KA
  TITLE     Nucleocytoplasmic transport of active HER2 causes fractional escape 
            from the DCIS-like state.
  JOURNAL   Nat Commun. 2023 Apr 13;14(1):2110. doi: 10.1038/s41467-023-37914-x.
  PUBMED    37055441
REFERENCE   2  (bases 1 to 14417)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-04-13"; volume: "14(1):2110. doi"; pages: " 
            10.1038/s41467-023-37914-x"
COMMENT     Sequence Label: pSLIK 3xFLAG-KIF20A zeo
FEATURES             Location/Qualifiers
     source          1..14417
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1680..1913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2098..2142
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2440..2557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    2614..2638
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        2670..2984
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    2678..2696
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2714..2732
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2749..2767
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2785..2803
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2821..2839
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2856..2874
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2892..2910
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2957..2981
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             2994..3059
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     protein_bind    complement(5768..5792)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        5842..7053
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     6890..6909
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             join(7112..7729,7730..7735,7736..7771,7772..7774,7775..7810,
                     7811..7813,7814..7849,7850..7858)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    7896..8469
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(8063..8080)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     8290..8309
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     promoter        8491..8538
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8557..8931
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     misc_feature    9105..9693
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9158..9178)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9576..9587)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(9696..9712)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9697..9713)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             10222..10402
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(10428..10445)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    10434..10658
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      10704..11132
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10791..10810)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     11001..11022
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(11141..11161)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        11146..11475
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      11326..11461
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     11388..11407
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        11523..11570
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             11589..11963
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    12093..12214
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(12130..12149)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     12184..12203
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(12263..12279)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12263..12279)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(12276..12298)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    12287..12303
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(12311..12317,12318..12335,12336..12341))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12356..12377
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(12494..12511)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(12665..13253)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(12745..12764)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(13424..14215,14216..14284))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     14047..14066
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(14285..14389)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaattc tagacagtta tcaacaagtt tgtacaaaaa agcaggcttt
     2641 aaaggaacca attcagtcga ctggatcccg agtttactcc ctatcagtga tagagaacgt
     2701 atgtcgagtt tactccctat cagtgataga gaacgatgtc gagtttactc cctatcagtg
     2761 atagagaacg tatgtcgagt ttactcccta tcagtgatag agaacgtatg tcgagtttac
     2821 tccctatcag tgatagagaa cgtatgtcga gtttatccct atcagtgata gagaacgtat
     2881 gtcgagttta ctccctatca gtgatagaga acgtatgtcg aggtaggcgt gtacggtggg
     2941 aggcctatat aagcagagct cgtttagtga accgtcagat cgccactagt atggactaca
     3001 aagaccatga cggtgattat aaagatcatg acatcgatta caaggatgac gatgacaaga
     3061 tgtcgcaagg gatcctttct ccgccagcgg gcttgctgtc cgatgacgat gtcgtagttt
     3121 ctcccatgtt tgagtccaca gctgcagatt tggggtctgt ggtacgcaag aacctgctat
     3181 cagactgctc tgtcgtctct acctccctag aggacaagca gcaggttcca tctgaggaca
     3241 gtatggagaa ggtgaaagta tacttgaggg ttaggccctt gttaccttca gagttggaac
     3301 gacaggaaga tcagggttgt gtccgtattg agaatgtgga gacccttgtt ctacaagcac
     3361 ccaaggactc ttttgccctg aagagcaatg aacggggaat tggccaagcc acacacaggt
     3421 tcaccttttc ccagatcttt gggccagaag tgggacaggc atccttcttc aacctaactg
     3481 tgaaggagat ggtaaaggat gtactcaaag ggcagaactg gctcatctat acatatggag
     3541 tcactaactc agggaaaacc cacacgattc aaggtaccat caaggatgga gggattctcc
     3601 cccggtccct ggcgctgatc ttcaatagcc tccaaggcca acttcatcca acacctgatc
     3661 tgaagccctt gctctccaat gaggtaatct ggctagacag caagcagatc cgacaggagg
     3721 aaatgaagaa gctgtccctg ctaaatggag gcctccaaga ggaggagctg tccacttcct
     3781 tgaagaggag tgtctacatc gaaagtcgga taggtaccag caccagcttc gacagtggca
     3841 ttgctgggct ctcttctatc agtcagtgta ccagcagtag ccagctggat gaaacaagtc
     3901 atcgatgggc acagccagac actgccccac tacctgtccc ggcaaacatt cgcttctcca
     3961 tctggatctc attctttgag atctacaacg aactgcttta tgacctatta gaaccgccta
     4021 gccaacagcg caagaggcag actttgcggc tatgcgagga tcaaaatggc aatccctatg
     4081 tgaaagatct caactggatt catgtgcaag atgctgagga ggcctggaag ctcctaaaag
     4141 tgggtcgtaa gaaccagagc tttgccagca cccacctcaa ccagaactcc agccgcagtc
     4201 acagcatctt ctcaatcagg atcctacacc ttcaggggga aggagatata gtccccaaga
     4261 tcagcgagct gtcactctgt gatctggctg gctcagagcg ctgcaaagat cagaagagtg
     4321 gtgaacggtt gaaggaagca ggaaacatta acacctctct acacaccctg ggccgctgta
     4381 ttgctgccct tcgtcaaaac cagcagaacc ggtcaaagca gaacctggtt cccttccgtg
     4441 acagcaagtt gactcgagtg ttccaaggtt tcttcacagg ccgaggccgt tcctgcatga
     4501 ttgtcaatgt gaatccctgt gcatctacct atgatgaaac tcttcatgtg gccaagttct
     4561 cagccattgc tagccagctt gtgcatgccc cacctatgca actgggattc ccatccctgc
     4621 actcgttcat caaggaacat agtcttcagg tatcccccag cttagagaaa ggggctaagg
     4681 cagacacagg ccttgatgat gatattgaaa atgaagctga catctccatg tatggcaaag
     4741 aggagctcct acaagttgtg gaagccatga agacactgct tttgaaggaa cgacaggaaa
     4801 agctacagct ggagatgcat ctccgagatg aaatttgcaa tgagatggta gaacagatgc
     4861 aacagcggga acagtggtgc agtgaacatt tggacaccca aaaggaacta ttggaggaaa
     4921 tgtatgaaga aaaactaaat atcctcaagg agtcactgac aagtttttac caagaagaga
     4981 ttcaggagcg ggatgaaaag attgaagagc tagaagctct cttgcaggaa gccagacaac
     5041 agtcagtggc ccatcagcaa tcagggtctg aattggccct acggcggtca caaaggttgg
     5101 cagcttctgc ctccacccag cagcttcagg aggttaaagc taaattacag cagtgcaaag
     5161 cagagctaaa ctctaccact gaagagttgc ataagtatca gaaaatgtta gaaccaccac
     5221 cctcagccaa gcccttcacc attgatgtgg acaagaagtt agaagagggc cagaagaata
     5281 taaggctgtt gcggacagag cttcagaaac ttggtgagtc tctccaatca gcagagagag
     5341 cttgttgcca cagcactggg gcaggaaaac ttcgtcaagc cttgaccact tgtgatgaca
     5401 tcttaatcaa acaggaccag actctggctg aactgcagaa caacatggtg ctagtgaaac
     5461 tggaccttcg gaagaaggca gcatgtattg ctgagcagta tcatactgtg ttgaaactcc
     5521 aaggccaggt ttctgccaaa aagcgccttg gtaccaacca ggaaaatcag caaccaaacc
     5581 aacaaccacc agggaagaaa ccattccttc gaaatttact tccccgaaca ccaacctgcc
     5641 aaagctcaac agactgcagc ccttatgccc ggatcctacg ctcacggcgt tcccctttac
     5701 tcaaatctgg gccttttggc aaaaagtact aacaattgga agactaatgc gttttcgaga
     5761 tatctagacc cagctttctt gtacaaagtg gttgataaca tctcgagcca taaagatggt
     5821 taattaaccc acccaagatc tggcctccgc gccgggtttt ggcgcctccc gcgggcgccc
     5881 ccctcctcac ggcgagcgct gccacgtcag acgaagggcg cagcgagcgt cctgatcctt
     5941 ccgcccggac gctcaggaca gcggcccgct gctcataaga ctcggcctta gaaccccagt
     6001 atcagcagaa ggacatttta ggacgggact tgggtgactc tagggcactg gttttctttc
     6061 cagagagcgg aacaggcgag gaaaagtagt cccttctcgg cgattctgcg gagggatctc
     6121 cgtggggcgg tgaacgccga tgattatata aggacgcgcc gggtgtggca cagctagttc
     6181 cgtcgcagcc gggatttggg tcgcggttct tgtttgtgga tcgctgtgat cgtcacttgg
     6241 tgagtagcgg gctgctgggc tggccggggc tttcgtggcc gccgggccgc tcggtgggac
     6301 ggaagcgtgt ggagagaccg ccaagggctg tagtctgggt ccgcgagcaa ggttgccctg
     6361 aactgggggt tggggggagc gcagcaaaat ggcggctgtt cccgagtctt gaatggaaga
     6421 cgcttgtgag gcgggctgtg aggtcgttga aacaaggtgg ggggcatggt gggcggcaag
     6481 aacccaaggt cttgaggcct tcgctaatgc gggaaagctc ttattcgggt gagatgggct
     6541 ggggcaccat ctggggaccc tgacgtgaag tttgtcactg actggagaac tcggtttgtc
     6601 gtctgttgcg ggggcggcag ttatggcggt gccgttgggc agtgcacccg tacctttggg
     6661 agcgcgcgcc ctcgtcgtgt cgtgacgtca cccgttctgt tggcttataa tgcagggtgg
     6721 ggccacctgc cggtaggtgt gcggtaggct tttctccgtc gcaggacgca gggttcgggc
     6781 ctagggtagg ctctcctgaa tcgacaggcg ccggacctct ggtgagggga gggataagtg
     6841 aggcgtcagt ttctttggtc ggttttatgt acctatcttc ttaagtagct gaagctccgg
     6901 ttttgaacta tgcgctcggg gttggcgagt gtgttttgtg aagtttttta ggcacctttt
     6961 gaaatgtaat catttgggtc aatatgtaat tttcagtgtt agactagtaa attgtccgct
     7021 aaattctggc cgtttttggc ttttttgtta gacgaagctt ggtaccgagc tcggatctcc
     7081 accccgtacc ggtcctgcag tcgaattcac catgtctaga ctggacaaga gcaaagtcat
     7141 aaacggagct ctggaattac tcaatggtgt cggtatcgaa ggcctgacga caaggaaact
     7201 cgctcaaaag ctgggagttg agcagcctac cctgtactgg cacgtgaaga acaagcgggc
     7261 cctgctcgat gccctgccaa tcgagatgct ggacaggcat catacccact tctgccccct
     7321 ggaaggcgag tcatggcaag actttctgcg gaacaacgcc aagtcatacc gctgtgctct
     7381 cctctcacat cgcgacgggg ctaaagtgca tctcggcacc cgcccaacag agaaacagta
     7441 cgaaaccctg gaaaatcagc tcgcgttcct gtgtcagcaa ggcttctccc tggagaacgc
     7501 actgtacgct ctgtccgccg tgggccactt tacactgggc tgcgtattgg aggaacagga
     7561 gcatcaagta gcaaaagagg aaagagagac acctaccacc gattctatgc ccccacttct
     7621 gagacaagca attgagctgt tcgaccggca gggagccgaa cctgccttcc ttttcggcct
     7681 ggaactaatc atatgtggcc tggagaaaca gctaaagtgc gaaagcggcg ggccgaccga
     7741 cgcccttgac gattttgact tagacatgct cccagccgat gcccttgacg actttgacct
     7801 tgatatgctg cctgctgacg ctcttgacga ttttgacctt gacatgctcc ccgggtaact
     7861 aagtaaggat ccgcggccgc actagaggaa ttccgcccct ctcccccccc cccccctaac
     7921 gttactggcc gaagccgctt ggaataaggc cggtgtgcgt ttgtctatat gttattttcc
     7981 accatattgc cgtcttttgg caatgtgagg gcccggaaac ctggccctgt cttcttgacg
     8041 agcattccta ggggtctttc ccctctcgcc aaaggaatgc aaggtctgtt gaatgtcgtg
     8101 aaggaagcag ttcctctgga agcttcttga agacaaacaa cgtctgtagc gaccctttgc
     8161 aggcagcgga accccccacc tggcgacagg tgcctctgcg gccaaaagcc acgtgtataa
     8221 gatacacctg caaaggcggc acaaccccag tgccacgttg tgagttggat agttgtggaa
     8281 agagtcaaat ggctctcctc aagcgtattc aacaaggggc tgaaggatgc ccagaaggta
     8341 ccccattgta tgggatctga tctggggcct cggtgcacat gctttacatg tgtttagtcg
     8401 aggttaaaaa aacgtctagg ccccccgaac cacggggacg tggttttcct ttgaaaaaca
     8461 cgatgataag cttaccggta cgcgtgatgt gttgacaatt aatcatcggc atagtatatc
     8521 ggcatagtat aatacgacaa ggtgaggaac taaaccatgg ccaagttgac cagtgccgtt
     8581 ccggtgctca ccgcgcgcga cgtcgccgga gcggtcgagt tctggaccga ccggctcggg
     8641 ttctcccggg acttcgtgga ggacgacttc gccggtgtgg tccgggacga cgtgaccctg
     8701 ttcatcagcg cggtccagga ccaggtggtg ccggacaaca ccctggcctg ggtgtgggtg
     8761 cgcggcctgg acgagctgta cgccgagtgg tcggaggtcg tgtccacgaa cttccgggac
     8821 gcctccgggc cggccatgac cgagatcggc gagcagccgt gggggcggga gttcgccctg
     8881 cgcgacccgg ccggcaactg cgtgcacttc gtggccgagg agcaggactg acacatctgt
     8941 acaagtaaag cggccgcgac tctagatcat aatcagccat accacatttg tagaggtttt
     9001 acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat
     9061 tgttgttgtt tagtccctcc caattcgata tcaagcttat cgataatcaa cctctggatt
     9121 acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg
     9181 gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct
     9241 cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc
     9301 aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca
     9361 ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac
     9421 tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt
     9481 ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt gttgccacct
     9541 ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc
     9601 cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga
     9661 cgagtcggat ctccctttgg gccgcctccc cgcatcgata ccgtcgacct cgatcgagac
     9721 ctagaaaaac atggagcaat cacaagtagc aatacagcag ctaccaatgc tgattgtgcc
     9781 tggctagaag cacaagagga ggaggaggtg ggttttccag tcacacctca ggtaccttta
     9841 agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga
     9901 ctggaagggc taattcactc ccaacgaaga caagatatcc ttgatctgtg gatctaccac
     9961 acacaaggct acttccctga ttggcagaac tacacaccag ggccagggat cagatatcca
    10021 ctgacctttg gatggtgcta caagctagta ccagttgagc aagagaaggt agaagaagcc
    10081 aatgaaggag agaacacccg cttgttacac cctgtgagcc tgcatgggat ggatgacccg
    10141 gagagagaag tattagagtg gaggtttgac agccgcctag catttcatca catggcccga
    10201 gagctgcatc cggactgtac tgggtctctc tggttagacc agatctgagc ctgggagctc
    10261 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa
    10321 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag
    10381 tcagtgtgga aaatctctag cagggcccgt ttaaacccgc tgatcagcct cgactgtgcc
    10441 ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg
    10501 tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag
    10561 gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga
    10621 caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccag
    10681 ctggggctct agggggtatc cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt
    10741 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc
    10801 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg
    10861 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta
    10921 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt
    10981 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat
    11041 ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa
    11101 tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg
    11161 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
    11221 tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
    11281 catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact
    11341 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
    11401 gccgaggccg cctctgcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
    11461 ctaggctttt gcaaaaagct cccgggagct tgtatatcca ttttcggatc tgatcagcac
    11521 gtgttgacaa ttaatcatcg gcatagtata tcggcatagt ataatacgac aaggtgagga
    11581 actaaaccat ggccaagttg accagtgccg ttccggtgct caccgcgcgc gacgtcgccg
    11641 gagcggtcga gttctggacc gaccggctcg ggttctcccg ggacttcgtg gaggacgact
    11701 tcgccggtgt ggtccgggac gacgtgaccc tgttcatcag cgcggtccag gaccaggtgg
    11761 tgccggacaa caccctggcc tgggtgtggg tgcgcggcct ggacgagctg tacgccgagt
    11821 ggtcggaggt cgtgtccacg aacttccggg acgcctccgg gccggccatg accgagatcg
    11881 gcgagcagcc gtgggggcgg gagttcgccc tgcgcgaccc ggccggcaac tgcgtgcact
    11941 tcgtggccga ggagcaggac tgacacgtgc tacgagattt cgattccacc gccgccttct
    12001 atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg
    12061 gggatctcat gctggagttc ttcgcccacc ccaacttgtt tattgcagct tataatggtt
    12121 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
    12181 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctgtataccg tcgacctcta
    12241 gctagagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca
    12301 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag
    12361 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
    12421 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
    12481 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
    12541 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
    12601 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
    12661 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
    12721 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
    12781 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
    12841 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
    12901 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
    12961 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
    13021 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
    13081 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
    13141 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
    13201 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
    13261 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
    13321 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
    13381 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
    13441 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
    13501 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
    13561 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
    13621 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
    13681 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
    13741 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
    13801 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
    13861 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
    13921 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
    13981 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
    14041 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
    14101 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
    14161 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
    14221 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
    14281 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
    14341 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
    14401 gaaaagtgcc acctgac
//
