LOCUS       sequence-375844-       10335 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  E. coli - Eggerthella lenta shuttle plasmid (KanR), cumate-inducible
            promoter Pct5 (engineered from pCT5-bac2.0)-LacZ.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10335)
  AUTHORS   Dong X, Guthrie BGH, Alexander M, Noecker C, Ramirez L, Glasser NR, 
            Turnbaugh PJ, Balskus EP
  TITLE     Genetic manipulation of the human gut bacterium Eggerthella lenta 
            reveals a widespread family of transcriptional regulators.
  JOURNAL   Nat Commun. 2022 Dec 9;13(1):7624. doi: 10.1038/s41467-022-33576-3.
  PUBMED    36494336
REFERENCE   2  (bases 1 to 10335)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-12-9"; volume: "13(1):7624. doi"; pages: " 
            10.1038/s41467-022-33576-3"
COMMENT     Sequence Label: pXD70CT5-PxylR-CymR, Pveg(CuO)-LacZ
FEATURES             Location/Qualifiers
     source          1..10335
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      33..104
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     rep_origin      complement(285..873)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(365..384)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1044..1835,1836..1904))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1667..1686
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1905..2009)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2077..2095
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    2252..2271
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that start with ""ATG"""
     CDS             complement(2274..2885)
                     /codon_start=1
                     /gene="cymR from Pseudomonas putida"
                     /product="cumate repressor (Mullick et al., 2006)"
                     /label=CymR
                     /translation="MSPKRRTQAERAMETQGKLIAAALGVLREKGYAGFRIADVPGAAG
                     VSRGAQSHHFPTKLELLLATFEWLYEQITERSRARLAKLKPEDDVIQQMLDDAAEFFLD
                     DDFSISLDLIVAADRDPALREGIQRTVERNRFVVEDMWLGVLVSRGLSRDDAEDILWLI
                     FNSVRGLAVRSLWQKDKERFERVRNSTLEIARERYAKFKR"
     protein_bind    3223..3250
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P2 operator sequence from the p-cmt 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     CDS             3279..6353
                     /codon_start=1
                     /gene="lacZ"
                     /product="beta-galactosidase"
                     /label=lacZ
                     /translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEART
                     DRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNV
                     TYPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYG
                     QDSRLPSEFDLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQ
                     ISDFHVATRFNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIID
                     ERGGYADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIEN
                     GLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLW
                     YTLCDRYGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWS
                     LGNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVP
                     KWSIKKWLSLPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSL
                     IKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQ
                     TIEVTSEYLFRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQ
                     LWLTVRVVQPNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELG
                     NKRWQFNRQSGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWK
                     AAGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDV
                     EVASDTPHPARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPY
                     VFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDG
                     FHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQK"
     primer_bind     complement(3299..3316)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3299..3315)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3308..3330)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     terminator      6371..6398
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             complement(6521..7315)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
ORIGIN
        1 ctgccgccaa gcttgctggg gcttttcttt ttcaaataaa acgaaaggct cagtcgaaag
       61 actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag aggagctcat
      121 gccggcggca cctcgctaac ggattcacca ctccaagaat tggagccaat caattcttgc
      181 ggagaactgt gaatgcgcaa accaaccctt ggcagaacat atccatcgcg tccgccatct
      241 ccagcagccg cacgcggcgc atctcgggcc gcgttgctgg cgtttttcca taggctccgc
      301 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
      361 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
      421 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
      481 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
      541 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
      601 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
      661 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
      721 agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
      781 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
      841 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
      901 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
      961 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     1021 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
     1081 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
     1141 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
     1201 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
     1261 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
     1321 tcgccagtta atagtttgcg caacgttgtt gccattgctg caggcatcgt ggtgtcacgc
     1381 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
     1441 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
     1501 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
     1561 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
     1621 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa cacgggataa taccgcgcca
     1681 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
     1741 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
     1801 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
     1861 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
     1921 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
     1981 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
     2041 taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt
     2101 cgtcttcaag gcgaaaaaac cccgccgaag cggggttttt tgcgaaatat aatgaccctc
     2161 ttgataaccc aagagggcat tttttaggaa cggttatttg attattaatc cggcggcaac
     2221 tgaaacgatt cggatcctgt attactattc tgaattcgcg gccgcttcta gatctagcgc
     2281 ttgaatttcg cgtaccgctc tcgcgcaatt tcgagtgtcg agttcctgac acgctcaaag
     2341 cgttctttgt ccttctgcca taggctacga acagcaagcc cacgcaccga attgaatatc
     2401 aaccaaagga tatcttctgc atcatcacgc gaaagaccac ggctcaccag aacaccaagc
     2461 cacatatcct cgacgacaaa ccgattcctc tctaccgtgc gctgaatacc ctcgcgtaac
     2521 gctgggtccc ggtcggcagc cacaatcaaa tcaaggctga tagagaagtc atcgtcgagg
     2581 aaaaattcgg cggcgtcgtc cagcatttgc tggatgacgt catcctctgg cttcaatttc
     2641 gctaatcgag cccgactgcg ttcggtgatc tgttcgtaaa gccattcaaa agtggcaagc
     2701 agaagctcaa gctttgtcgg gaaatgatgg ctctgcgctc ctctggagac accagcagca
     2761 ccgggcacat ctgcgatccg gaatcccgcg taaccttttt cccgtaaaac ccccagggcc
     2821 gctgcaatca acttgccctg ggtctccatt gcgcgctctg cctgtgttct tctctttgga
     2881 ctcatgatca ccattttttt tactaaagct tgatctgcaa tttgaataat aaccactcct
     2941 ttgtttatcc accgaactaa gttggtgttt tttgaagctt gaattagata tttaaaagta
     3001 tcatatctaa tattataact aaattttcta aaaaaaacat tgaaggaacg gttatttgat
     3061 tattaatccg gcttttttat tatttaggca actgaaacga ttcggatcct gtattactat
     3121 tctattttgt caaaataatt ttattgacaa cgtcttatta acgttgatat aatttaaatt
     3181 ttatttgaca aaaatgggct cgtgttgtac aataaatgta gtaacaaaca gacaatctgg
     3241 tctgtttgta ttatgattaa ctaataagga ggacaaacat gaccatgatt acggattcac
     3301 tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc
     3361 ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc
     3421 cttcccaaca gttgcgcagc ctgaatggcg aatggcgctt tgcctggttt ccggcaccag
     3481 aagcggtgcc ggaaagctgg ctggagtgcg atcttcctga ggccgatact gtcgtcgtcc
     3541 cctcaaactg gcagatgcac ggttacgatg cgcccatcta caccaacgtg acctatccca
     3601 ttacggtcaa tccgccgttt gttcccacgg agaatccgac gggttgttac tcgctcacat
     3661 ttaatgttga tgaaagctgg ctacaggaag gccagacgcg aattattttt gatggcgtta
     3721 actcggcgtt tcatctgtgg tgcaacgggc gctgggtcgg ttacggccag gacagtcgtt
     3781 tgccgtctga atttgacctg agcgcatttt tacgcgccgg agaaaaccgc ctcgcggtga
     3841 tggtgctgcg ctggagtgac ggcagttatc tggaagatca ggatatgtgg cggatgagcg
     3901 gcattttccg tgacgtctcg ttgctgcata aaccgactac acaaatcagc gatttccatg
     3961 ttgccactcg ctttaatgat gatttcagcc gcgctgtact ggaggctgaa gttcagatgt
     4021 gcggcgagtt gcgtgactac ctacgggtaa cagtttcttt atggcagggt gaaacgcagg
     4081 tcgccagcgg caccgcgcct ttcggcggtg aaattatcga tgagcgtggt ggttatgccg
     4141 atcgcgtcac actacgtctg aacgtcgaaa acccgaaact gtggagcgcc gaaatcccga
     4201 atctctatcg tgcggtggtt gaactgcaca ccgccgacgg cacgctgatt gaagcagaag
     4261 cctgcgatgt cggtttccgc gaggtgcgga ttgaaaatgg tctgctgctg ctgaacggca
     4321 agccgttgct gattcgaggc gttaaccgtc acgagcatca tcctctgcat ggtcaggtca
     4381 tggatgagca gacgatggtg caggatatcc tgctgatgaa gcagaacaac tttaacgccg
     4441 tgcgctgttc gcattatccg aaccatccgc tgtggtacac gctgtgcgac cgctacggcc
     4501 tgtatgtggt ggatgaagcc aatattgaaa cccacggcat ggtgccaatg aatcgtctga
     4561 ccgatgatcc gcgctggcta ccggcgatga gcgaacgcgt aacgcgaatg gtgcagcgcg
     4621 atcgtaatca cccgagtgtg atcatctggt cgctggggaa tgaatcaggc cacggcgcta
     4681 atcacgacgc gctgtatcgc tggatcaaat ctgtcgatcc ttcccgcccg gtgcagtatg
     4741 aaggcggcgg agccgacacc acggccaccg atattatttg cccgatgtac gcgcgcgtgg
     4801 atgaagacca gcccttcccg gctgtgccga aatggtccat caaaaaatgg ctttcgctac
     4861 ctggagagac gcgcccgctg atcctttgcg aatacgccca cgcgatgggt aacagtcttg
     4921 gcggtttcgc taaatactgg caggcgtttc gtcagtatcc ccgtttacag ggcggcttcg
     4981 tctgggactg ggtggatcag tcgctgatta aatatgatga aaacggcaac ccgtggtcgg
     5041 cttacggcgg tgattttggc gatacgccga acgatcgcca gttctgtatg aacggtctgg
     5101 tctttgccga ccgcacgccg catccagcgc tgacggaagc aaaacaccag cagcagtttt
     5161 tccagttccg tttatccggg caaaccatcg aagtgaccag cgaatacctg ttccgtcata
     5221 gcgataacga gctcctgcac tggatggtgg cgctggatgg taagccgctg gcaagcggtg
     5281 aagtgcctct ggatgtcgct ccacaaggta aacagttgat tgaactgcct gaactaccgc
     5341 agccggagag cgccgggcaa ctctggctca cagtacgcgt agtgcaaccg aacgcgaccg
     5401 catggtcaga agccgggcac atcagcgcct ggcagcagtg gcgtctggcg gaaaacctca
     5461 gtgtgacgct ccccgccgcg tcccacgcca tcccgcatct gaccaccagc gaaatggatt
     5521 tttgcatcga gctgggtaat aagcgttggc aatttaaccg ccagtcaggc tttctttcac
     5581 agatgtggat tggcgataaa aaacaactgc tgacgccgct gcgcgatcag ttcacccgtg
     5641 caccgctgga taacgacatt ggcgtaagtg aagcgacccg cattgaccct aacgcctggg
     5701 tcgaacgctg gaaggcggcg ggccattacc aggccgaagc agcgttgttg cagtgcacgg
     5761 cagatacact tgctgatgcg gtgctgatta cgaccgctca cgcgtggcag catcagggga
     5821 aaaccttatt tatcagccgg aaaacctacc ggattgatgg tagtggtcaa atggcgatta
     5881 ccgttgatgt tgaagtggcg agcgatacac cgcatccggc gcggattggc ctgaactgcc
     5941 agctggcgca ggtagcagag cgggtaaact ggctcggatt agggccgcaa gaaaactatc
     6001 ccgaccgcct tactgccgcc tgttttgacc gctgggatct gccattgtca gacatgtata
     6061 ccccgtacgt cttcccgagc gaaaacggtc tgcgctgcgg gacgcgcgaa ttgaattatg
     6121 gcccacacca gtggcgcggc gacttccagt tcaacatcag ccgctacagt caacagcaac
     6181 tgatggaaac cagccatcgc catctgctgc acgcggaaga aggcacatgg ctgaatatcg
     6241 acggtttcca tatggggatt ggtggcgacg actcctggag cccgtcagta tcggcggaat
     6301 tccagctgag cgccggtcgc taccattacc agttggtctg gtgtcaaaaa taaactagtc
     6361 gactcacact ggctcacctt cgggtgggcc tttctgcgtt tatatactag agagagaata
     6421 taaaaagcca gattattaat ccggcttttt tattatttaa ttcctgttat aaaaaaagga
     6481 tcaattttga actctctccc aaagttgatc ccttaacgat ctaaaacaat tcatccagta
     6541 aaatataata ttttattttc tcccaatcag gcttgatccc cagtaagtca aaaaatagct
     6601 cgacatactg ttcttccccg atatcctccc tgatcgaccg gacgcagaag gcaatgtcat
     6661 accacttgtc cgccctgccg cttctcccaa gatcaataaa gccacttact ttgccatctt
     6721 tcacaaagat gttgctgtct cccaggtcgc cgtgggaaaa gacaagttcc tcttcgggct
     6781 tttccgtctt taaaaaatca tacagctcgc gcggatcttt aaatggagtg tcttcttccc
     6841 agttttcgca atccacatcg gccagatcgt tattcagtaa gtaatccaat tcggctaagc
     6901 ggctgtctaa gctattcgta tagggacaat ccgatatgtc gatggagtga aagagcctga
     6961 tgcactccgc atacagctcg ataatctttt cagggctttg ttcatcttca tactcttccg
     7021 agcaaaggac gccatcggcc tcactcatga gcagattgct ccagccatca tgccgttcaa
     7081 agtgcaggac ctttggaaca ggcagctttc cttccagcca tagcatcatg tccttttccc
     7141 gttccacatc ataggtggtc cctttatacc ggctgtccgt catttttaaa tataggtttt
     7201 cattttctcc caccagctta tataccttag caggagacat tccttccgta tcttttacgc
     7261 agcggtattt ttcgatcagt tttttcaatt ccggtgatat tctcatttta gccatttatt
     7321 atttccttcc tcttttctac agtatttaaa gataccccaa gaagctaatt ataacaagac
     7381 gaactccaat tcactgttcc ttgcattcta aaaccttaaa taccagaaaa cagctttttc
     7441 aaagttgttt tcaaagttgg cgtataacat agtatcgacg gagccgattt tgaaaccaca
     7501 attatgatag aatttacaag ctataaggtt attgtcctgg gtttcaagca ttagtccatg
     7561 caagttttta tgctttgccc attctataga tatattgata agaagactgg aatgcccgct
     7621 ccgccgggat tattcgaaag cgcatcgtcc aagcgagacc gtctgcattc ggaaatctcg
     7681 gccggttcgc gccgaggaag cccatctggc tgcatcccaa atcgacacac aacgcacgga
     7741 ggccccggcg atcgccgggg cctccgaatc atgcgcatcg tggaagcgaa gctccgcaaa
     7801 aaaccccgct tcagcggggt tttttcgcgt cgcacctcct tgtcgtgtat tacaggtact
     7861 actataacac atcatctagc ccgcccgcga tacgtcctct gcggtgcagc gtggtcgcgg
     7921 ttccacgcat ccgccgcccg ctgcgcatcg cgcagcacgt cattaaggct ctgttgcctt
     7981 ggctgttccc tgcgcatcag atattcgagc gggcgcgata ttcgacttct aagctcttcc
     8041 actttgattc gcattcccgc aactcctgct ctgatgcggc tcgcgagtcc atcgcactta
     8101 acaacgatgt tatcgaggat tgcgccgcgc tcagcttttc ccgcagcttc ccagccccga
     8161 acctcggtag cgatgggttg aagctctcca atgtctcgcg caactccgtc cgctcgttgc
     8221 cgaagacact ccaatcggtc ttgctcatct tcgatatcga gcttcacgtc ctcgagctgg
     8281 ctgtcaagct cggcaaggtc accctccttg tcggcaatct cggtaacaag ctctgcctta
     8341 cgcgtttcgg catcattcag taaagcggcg gcttcttccc tagccgccgc gacgattcgc
     8401 gccgcttcgt ccttcgcagg ttcaaccacg gccttatcga ccgcacagcg taccttgtcg
     8461 atatccacgg acttgtccgt gtatacacgc tgcgcccgcg tctcctcgtc aagctcgatt
     8521 gcagggcggt agcccatgcg cccctctagg taatccccaa gctccttgtg catgctccgg
     8581 tagaagctgc gggggcacat ctgcttgtag ttgaaccgtc catctaggat gggcgtgaac
     8641 ggcacgtgca tgtggtcgcg ggcgggcgtg ccatccttca acacctcgtc cttgtggacg
     8701 tacccgccca tcatgttctc gatgccgaac ttcttcgaca ggtaccagta cgtgagacgg
     8761 aagaacctgt cctcatcgcc cttgcgcaca ttgtcgggaa gcgtcacaac aacgtcagcc
     8821 ataacgaccg catccttgcg cgtggcacgc ttccctgcgg ccttctgggc ctcgttcacg
     8881 cgctcgatgc ggctctccac cgtgccccag ttcggctcgc catcgcgcgg ctccacgcgc
     8941 cccacaacga gcttgccgtc cttgttcgtg tagtggccga ccaccaagtc gttcttgatg
     9001 cgctcggggt cgatgttgtc gcgctccagg ctgctgcggt cgcggcggta gtgggcaagc
     9061 atgtgcccga ccgatggagc cttgtatttc gcgatgtgag ccatggaaac ctccgaaccg
     9121 tacagactta atcgactaaa gtctagcacg ttagacctta gctacgctaa ggacgtggca
     9181 gggccagccc tgcacccgct cgggcgttcc ccccgagacc ccccagccct tgcccgttcg
     9241 cggcttcgct cacggccctc gggccatcgg cgcgagcgcc gaacggaggg acttcgcccc
     9301 tccgaaccac ccctcaatct acctgcggtt cggcacacgg cacacggcac tattaggggg
     9361 gccgtcgtag tggcccccct gtggtcattt gtcatttgtc atttgtcaca ttatttttcg
     9421 gacacattat ttttcggacg tagttagcga aaaataatga gccgcccgat tctgcaggtc
     9481 tcatccagtc gagacacggg caaggaaagg gtcgatggag cagagcgtcc atcgaccctt
     9541 aatcaagtct ggtctattcg gttggccgtc gcgtcaattc cgcaatccgt gaacgagtcg
     9601 gcttagatct gcgcgagcgc agaccacgcc gccccgtagt cacgcattgc tgcattgacc
     9661 cgactactct ttggcacctg actgttccac gcacttgcgc ttacgttcgg cgttcagata
     9721 gttcccgcga caatagcgct cgagaatcga gttgtacccc acgatgattt cgcggtactg
     9781 ctcgggaaac tccctcagcg ccgcagccgt gaaatcgttg atggtctcga acctctcgca
     9841 catgatgaag ccgaccaact cgaagagcat gtcctgcttg tccttagtcg agagctgttc
     9901 gagctgacca atgtcgaagt tgtttccctc gatgcgtgct tcgaccggat acagctcctt
     9961 gcccttcttg cgacaggaag ccgtgtcgtg aatcagatag tcatacgcat gcctgatgtt
    10021 gttgattggc tcggctgacg aacagcactt cttgccgtcc gcgctcaatc ggttcaagac
    10081 gttgatgatt gccttgcgtg tcgtgttgcc gccccagaca acaatgacgt gcgcatgcgt
    10141 cttgcgctgc ttgctcttct ggtcatggtc tatgtcgtga accgcataag cgaaaggcac
    10201 ttgaagaatg tcgtctatct cgtcctgcca gtcatcgcgc atgttctctg ggtagcagat
    10261 gccctcccag agcttcacct tcgtctcgcc agcgtttgcc gccatgctat aatctccttg
    10321 tctcgaatgc ccctg
//
