LOCUS       sequence-375845-       10377 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  E. coli - Eggerthella lenta shuttle plasmid (KanR), cumate-inducible
            promoter Pct5.1 (tandem CymR binding sites)-LacZ.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10377)
  AUTHORS   Dong X, Guthrie BGH, Alexander M, Noecker C, Ramirez L, Glasser NR, 
            Turnbaugh PJ, Balskus EP
  TITLE     Genetic manipulation of the human gut bacterium Eggerthella lenta 
            reveals a widespread family of transcriptional regulators.
  JOURNAL   Nat Commun. 2022 Dec 9;13(1):7624. doi: 10.1038/s41467-022-33576-3.
  PUBMED    36494336
REFERENCE   2  (bases 1 to 10377)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-12-9"; volume: "13(1):7624. doi"; pages: " 
            10.1038/s41467-022-33576-3"
COMMENT     Sequence Label: pXD70CT5.1-PxylR-CymR, Pveg(2CuO)-LacZ
FEATURES             Location/Qualifiers
     source          1..10377
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      38..109
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     rep_origin      complement(290..878)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(370..389)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1049..1840,1841..1909))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1672..1691
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1910..2014)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2082..2100
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    2257..2276
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that start with ""ATG"""
     CDS             complement(2279..2890)
                     /codon_start=1
                     /gene="cymR from Pseudomonas putida"
                     /product="cumate repressor (Mullick et al., 2006)"
                     /label=CymR
                     /translation="MSPKRRTQAERAMETQGKLIAAALGVLREKGYAGFRIADVPGAAG
                     VSRGAQSHHFPTKLELLLATFEWLYEQITERSRARLAKLKPEDDVIQQMLDDAAEFFLD
                     DDFSISLDLIVAADRDPALREGIQRTVERNRFVVEDMWLGVLVSRGLSRDDAEDILWLI
                     FNSVRGLAVRSLWQKDKERFERVRNSTLEIARERYAKFKR"
     protein_bind    3228..3255
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P2 operator sequence from the p-cmt 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    3270..3297
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P2 operator sequence from the p-cmt 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     CDS             3326..6400
                     /codon_start=1
                     /gene="lacZ"
                     /product="beta-galactosidase"
                     /label=lacZ
                     /translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEART
                     DRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNV
                     TYPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYG
                     QDSRLPSEFDLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQ
                     ISDFHVATRFNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIID
                     ERGGYADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIEN
                     GLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLW
                     YTLCDRYGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWS
                     LGNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVP
                     KWSIKKWLSLPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSL
                     IKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQ
                     TIEVTSEYLFRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQ
                     LWLTVRVVQPNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELG
                     NKRWQFNRQSGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWK
                     AAGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDV
                     EVASDTPHPARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPY
                     VFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDG
                     FHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQK"
     primer_bind     complement(3346..3363)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3346..3362)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3355..3377)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     terminator      6418..6445
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             complement(6568..7362)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
ORIGIN
        1 ccctgctgcc gccaagcttg ctggggcttt tctttttcaa ataaaacgaa aggctcagtc
       61 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagaggag
      121 ctcatgccgg cggcacctcg ctaacggatt caccactcca agaattggag ccaatcaatt
      181 cttgcggaga actgtgaatg cgcaaaccaa cccttggcag aacatatcca tcgcgtccgc
      241 catctccagc agccgcacgc ggcgcatctc gggccgcgtt gctggcgttt ttccataggc
      301 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
      361 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
      421 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
      481 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
      541 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
      601 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
      661 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
      721 acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
      781 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
      841 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
      901 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
      961 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     1021 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
     1081 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
     1141 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
     1201 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
     1261 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
     1321 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctgcaggc atcgtggtgt
     1381 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
     1441 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
     1501 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
     1561 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
     1621 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaacacgg gataataccg
     1681 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
     1741 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
     1801 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
     1861 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
     1921 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
     1981 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
     2041 acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc
     2101 cctttcgtct tcaaggcgaa aaaaccccgc cgaagcgggg ttttttgcga aatataatga
     2161 ccctcttgat aacccaagag ggcatttttt aggaacggtt atttgattat taatccggcg
     2221 gcaactgaaa cgattcggat cctgtattac tattctgaat tcgcggccgc ttctagatct
     2281 agcgcttgaa tttcgcgtac cgctctcgcg caatttcgag tgtcgagttc ctgacacgct
     2341 caaagcgttc tttgtccttc tgccataggc tacgaacagc aagcccacgc accgaattga
     2401 atatcaacca aaggatatct tctgcatcat cacgcgaaag accacggctc accagaacac
     2461 caagccacat atcctcgacg acaaaccgat tcctctctac cgtgcgctga ataccctcgc
     2521 gtaacgctgg gtcccggtcg gcagccacaa tcaaatcaag gctgatagag aagtcatcgt
     2581 cgaggaaaaa ttcggcggcg tcgtccagca tttgctggat gacgtcatcc tctggcttca
     2641 atttcgctaa tcgagcccga ctgcgttcgg tgatctgttc gtaaagccat tcaaaagtgg
     2701 caagcagaag ctcaagcttt gtcgggaaat gatggctctg cgctcctctg gagacaccag
     2761 cagcaccggg cacatctgcg atccggaatc ccgcgtaacc tttttcccgt aaaaccccca
     2821 gggccgctgc aatcaacttg ccctgggtct ccattgcgcg ctctgcctgt gttcttctct
     2881 ttggactcat gatcaccatt ttttttacta aagcttgatc tgcaatttga ataataacca
     2941 ctcctttgtt tatccaccga actaagttgg tgttttttga agcttgaatt agatatttaa
     3001 aagtatcata tctaatatta taactaaatt ttctaaaaaa aacattgaag gaacggttat
     3061 ttgattatta atccggcttt tttattattt aggcaactga aacgattcgg atcctgtatt
     3121 actattctat tttgtcaaaa taattttatt gacaacgtct tattaacgtt gatataattt
     3181 aaattttatt tgacaaaaat gggctcgtgt tgtacaataa atgtagtaac aaacagacaa
     3241 tctggtctgt ttgtacgagt gttgcggcca acaaacagac aatctggtct gtttgtatta
     3301 tgattaacta ataaggagga caaacatgac catgattacg gattcactgg ccgtcgtttt
     3361 acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc
     3421 ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt
     3481 gcgcagcctg aatggcgaat ggcgctttgc ctggtttccg gcaccagaag cggtgccgga
     3541 aagctggctg gagtgcgatc ttcctgaggc cgatactgtc gtcgtcccct caaactggca
     3601 gatgcacggt tacgatgcgc ccatctacac caacgtgacc tatcccatta cggtcaatcc
     3661 gccgtttgtt cccacggaga atccgacggg ttgttactcg ctcacattta atgttgatga
     3721 aagctggcta caggaaggcc agacgcgaat tatttttgat ggcgttaact cggcgtttca
     3781 tctgtggtgc aacgggcgct gggtcggtta cggccaggac agtcgtttgc cgtctgaatt
     3841 tgacctgagc gcatttttac gcgccggaga aaaccgcctc gcggtgatgg tgctgcgctg
     3901 gagtgacggc agttatctgg aagatcagga tatgtggcgg atgagcggca ttttccgtga
     3961 cgtctcgttg ctgcataaac cgactacaca aatcagcgat ttccatgttg ccactcgctt
     4021 taatgatgat ttcagccgcg ctgtactgga ggctgaagtt cagatgtgcg gcgagttgcg
     4081 tgactaccta cgggtaacag tttctttatg gcagggtgaa acgcaggtcg ccagcggcac
     4141 cgcgcctttc ggcggtgaaa ttatcgatga gcgtggtggt tatgccgatc gcgtcacact
     4201 acgtctgaac gtcgaaaacc cgaaactgtg gagcgccgaa atcccgaatc tctatcgtgc
     4261 ggtggttgaa ctgcacaccg ccgacggcac gctgattgaa gcagaagcct gcgatgtcgg
     4321 tttccgcgag gtgcggattg aaaatggtct gctgctgctg aacggcaagc cgttgctgat
     4381 tcgaggcgtt aaccgtcacg agcatcatcc tctgcatggt caggtcatgg atgagcagac
     4441 gatggtgcag gatatcctgc tgatgaagca gaacaacttt aacgccgtgc gctgttcgca
     4501 ttatccgaac catccgctgt ggtacacgct gtgcgaccgc tacggcctgt atgtggtgga
     4561 tgaagccaat attgaaaccc acggcatggt gccaatgaat cgtctgaccg atgatccgcg
     4621 ctggctaccg gcgatgagcg aacgcgtaac gcgaatggtg cagcgcgatc gtaatcaccc
     4681 gagtgtgatc atctggtcgc tggggaatga atcaggccac ggcgctaatc acgacgcgct
     4741 gtatcgctgg atcaaatctg tcgatccttc ccgcccggtg cagtatgaag gcggcggagc
     4801 cgacaccacg gccaccgata ttatttgccc gatgtacgcg cgcgtggatg aagaccagcc
     4861 cttcccggct gtgccgaaat ggtccatcaa aaaatggctt tcgctacctg gagagacgcg
     4921 cccgctgatc ctttgcgaat acgcccacgc gatgggtaac agtcttggcg gtttcgctaa
     4981 atactggcag gcgtttcgtc agtatccccg tttacagggc ggcttcgtct gggactgggt
     5041 ggatcagtcg ctgattaaat atgatgaaaa cggcaacccg tggtcggctt acggcggtga
     5101 ttttggcgat acgccgaacg atcgccagtt ctgtatgaac ggtctggtct ttgccgaccg
     5161 cacgccgcat ccagcgctga cggaagcaaa acaccagcag cagtttttcc agttccgttt
     5221 atccgggcaa accatcgaag tgaccagcga atacctgttc cgtcatagcg ataacgagct
     5281 cctgcactgg atggtggcgc tggatggtaa gccgctggca agcggtgaag tgcctctgga
     5341 tgtcgctcca caaggtaaac agttgattga actgcctgaa ctaccgcagc cggagagcgc
     5401 cgggcaactc tggctcacag tacgcgtagt gcaaccgaac gcgaccgcat ggtcagaagc
     5461 cgggcacatc agcgcctggc agcagtggcg tctggcggaa aacctcagtg tgacgctccc
     5521 cgccgcgtcc cacgccatcc cgcatctgac caccagcgaa atggattttt gcatcgagct
     5581 gggtaataag cgttggcaat ttaaccgcca gtcaggcttt ctttcacaga tgtggattgg
     5641 cgataaaaaa caactgctga cgccgctgcg cgatcagttc acccgtgcac cgctggataa
     5701 cgacattggc gtaagtgaag cgacccgcat tgaccctaac gcctgggtcg aacgctggaa
     5761 ggcggcgggc cattaccagg ccgaagcagc gttgttgcag tgcacggcag atacacttgc
     5821 tgatgcggtg ctgattacga ccgctcacgc gtggcagcat caggggaaaa ccttatttat
     5881 cagccggaaa acctaccgga ttgatggtag tggtcaaatg gcgattaccg ttgatgttga
     5941 agtggcgagc gatacaccgc atccggcgcg gattggcctg aactgccagc tggcgcaggt
     6001 agcagagcgg gtaaactggc tcggattagg gccgcaagaa aactatcccg accgccttac
     6061 tgccgcctgt tttgaccgct gggatctgcc attgtcagac atgtataccc cgtacgtctt
     6121 cccgagcgaa aacggtctgc gctgcgggac gcgcgaattg aattatggcc cacaccagtg
     6181 gcgcggcgac ttccagttca acatcagccg ctacagtcaa cagcaactga tggaaaccag
     6241 ccatcgccat ctgctgcacg cggaagaagg cacatggctg aatatcgacg gtttccatat
     6301 ggggattggt ggcgacgact cctggagccc gtcagtatcg gcggaattcc agctgagcgc
     6361 cggtcgctac cattaccagt tggtctggtg tcaaaaataa actagtcgac tcacactggc
     6421 tcaccttcgg gtgggccttt ctgcgtttat atactagaga gagaatataa aaagccagat
     6481 tattaatccg gcttttttat tatttaattc ctgttataaa aaaaggatca attttgaact
     6541 ctctcccaaa gttgatccct taacgatcta aaacaattca tccagtaaaa tataatattt
     6601 tattttctcc caatcaggct tgatccccag taagtcaaaa aatagctcga catactgttc
     6661 ttccccgata tcctccctga tcgaccggac gcagaaggca atgtcatacc acttgtccgc
     6721 cctgccgctt ctcccaagat caataaagcc acttactttg ccatctttca caaagatgtt
     6781 gctgtctccc aggtcgccgt gggaaaagac aagttcctct tcgggctttt ccgtctttaa
     6841 aaaatcatac agctcgcgcg gatctttaaa tggagtgtct tcttcccagt tttcgcaatc
     6901 cacatcggcc agatcgttat tcagtaagta atccaattcg gctaagcggc tgtctaagct
     6961 attcgtatag ggacaatccg atatgtcgat ggagtgaaag agcctgatgc actccgcata
     7021 cagctcgata atcttttcag ggctttgttc atcttcatac tcttccgagc aaaggacgcc
     7081 atcggcctca ctcatgagca gattgctcca gccatcatgc cgttcaaagt gcaggacctt
     7141 tggaacaggc agctttcctt ccagccatag catcatgtcc ttttcccgtt ccacatcata
     7201 ggtggtccct ttataccggc tgtccgtcat ttttaaatat aggttttcat tttctcccac
     7261 cagcttatat accttagcag gagacattcc ttccgtatct tttacgcagc ggtatttttc
     7321 gatcagtttt ttcaattccg gtgatattct cattttagcc atttattatt tccttcctct
     7381 tttctacagt atttaaagat accccaagaa gctaattata acaagacgaa ctccaattca
     7441 ctgttccttg cattctaaaa ccttaaatac cagaaaacag ctttttcaaa gttgttttca
     7501 aagttggcgt ataacatagt atcgacggag ccgattttga aaccacaatt atgatagaat
     7561 ttacaagcta taaggttatt gtcctgggtt tcaagcatta gtccatgcaa gtttttatgc
     7621 tttgcccatt ctatagatat attgataaga agactggaat gcccgctccg ccgggattat
     7681 tcgaaagcgc atcgtccaag cgagaccgtc tgcattcgga aatctcggcc ggttcgcgcc
     7741 gaggaagccc atctggctgc atcccaaatc gacacacaac gcacggaggc cccggcgatc
     7801 gccggggcct ccgaatcatg cgcatcgtgg aagcgaagct ccgcaaaaaa ccccgcttca
     7861 gcggggtttt ttcgcgtcgc acctccttgt cgtgtattac aggtactact ataacacatc
     7921 atctagcccg cccgcgatac gtcctctgcg gtgcagcgtg gtcgcggttc cacgcatccg
     7981 ccgcccgctg cgcatcgcgc agcacgtcat taaggctctg ttgccttggc tgttccctgc
     8041 gcatcagata ttcgagcggg cgcgatattc gacttctaag ctcttccact ttgattcgca
     8101 ttcccgcaac tcctgctctg atgcggctcg cgagtccatc gcacttaaca acgatgttat
     8161 cgaggattgc gccgcgctca gcttttcccg cagcttccca gccccgaacc tcggtagcga
     8221 tgggttgaag ctctccaatg tctcgcgcaa ctccgtccgc tcgttgccga agacactcca
     8281 atcggtcttg ctcatcttcg atatcgagct tcacgtcctc gagctggctg tcaagctcgg
     8341 caaggtcacc ctccttgtcg gcaatctcgg taacaagctc tgccttacgc gtttcggcat
     8401 cattcagtaa agcggcggct tcttccctag ccgccgcgac gattcgcgcc gcttcgtcct
     8461 tcgcaggttc aaccacggcc ttatcgaccg cacagcgtac cttgtcgata tccacggact
     8521 tgtccgtgta tacacgctgc gcccgcgtct cctcgtcaag ctcgattgca gggcggtagc
     8581 ccatgcgccc ctctaggtaa tccccaagct ccttgtgcat gctccggtag aagctgcggg
     8641 ggcacatctg cttgtagttg aaccgtccat ctaggatggg cgtgaacggc acgtgcatgt
     8701 ggtcgcgggc gggcgtgcca tccttcaaca cctcgtcctt gtggacgtac ccgcccatca
     8761 tgttctcgat gccgaacttc ttcgacaggt accagtacgt gagacggaag aacctgtcct
     8821 catcgccctt gcgcacattg tcgggaagcg tcacaacaac gtcagccata acgaccgcat
     8881 ccttgcgcgt ggcacgcttc cctgcggcct tctgggcctc gttcacgcgc tcgatgcggc
     8941 tctccaccgt gccccagttc ggctcgccat cgcgcggctc cacgcgcccc acaacgagct
     9001 tgccgtcctt gttcgtgtag tggccgacca ccaagtcgtt cttgatgcgc tcggggtcga
     9061 tgttgtcgcg ctccaggctg ctgcggtcgc ggcggtagtg ggcaagcatg tgcccgaccg
     9121 atggagcctt gtatttcgcg atgtgagcca tggaaacctc cgaaccgtac agacttaatc
     9181 gactaaagtc tagcacgtta gaccttagct acgctaagga cgtggcaggg ccagccctgc
     9241 acccgctcgg gcgttccccc cgagaccccc cagcccttgc ccgttcgcgg cttcgctcac
     9301 ggccctcggg ccatcggcgc gagcgccgaa cggagggact tcgcccctcc gaaccacccc
     9361 tcaatctacc tgcggttcgg cacacggcac acggcactat taggggggcc gtcgtagtgg
     9421 cccccctgtg gtcatttgtc atttgtcatt tgtcacatta tttttcggac acattatttt
     9481 tcggacgtag ttagcgaaaa ataatgagcc gcccgattct gcaggtctca tccagtcgag
     9541 acacgggcaa ggaaagggtc gatggagcag agcgtccatc gacccttaat caagtctggt
     9601 ctattcggtt ggccgtcgcg tcaattccgc aatccgtgaa cgagtcggct tagatctgcg
     9661 cgagcgcaga ccacgccgcc ccgtagtcac gcattgctgc attgacccga ctactctttg
     9721 gcacctgact gttccacgca cttgcgctta cgttcggcgt tcagatagtt cccgcgacaa
     9781 tagcgctcga gaatcgagtt gtaccccacg atgatttcgc ggtactgctc gggaaactcc
     9841 ctcagcgccg cagccgtgaa atcgttgatg gtctcgaacc tctcgcacat gatgaagccg
     9901 accaactcga agagcatgtc ctgcttgtcc ttagtcgaga gctgttcgag ctgaccaatg
     9961 tcgaagttgt ttccctcgat gcgtgcttcg accggataca gctccttgcc cttcttgcga
    10021 caggaagccg tgtcgtgaat cagatagtca tacgcatgcc tgatgttgtt gattggctcg
    10081 gctgacgaac agcacttctt gccgtccgcg ctcaatcggt tcaagacgtt gatgattgcc
    10141 ttgcgtgtcg tgttgccgcc ccagacaaca atgacgtgcg catgcgtctt gcgctgcttg
    10201 ctcttctggt catggtctat gtcgtgaacc gcataagcga aaggcacttg aagaatgtcg
    10261 tctatctcgt cctgccagtc atcgcgcatg ttctctgggt agcagatgcc ctcccagagc
    10321 ttcaccttcg tctcgccagc gtttgccgcc atgctataat ctccttgtct cgaatgc
//
