LOCUS       sequence-375847-       12608 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression vector for an inducible 3xFLAG-BirA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12608)
  AUTHORS   Wang L, Paudel BB, McKnight RA, Janes KA
  TITLE     Nucleocytoplasmic transport of active HER2 causes fractional escape 
            from the DCIS-like state.
  JOURNAL   Nat Commun. 2023 Apr 13;14(1):2110. doi: 10.1038/s41467-023-37914-x.
  PUBMED    37055441
REFERENCE   2  (bases 1 to 12608)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-04-13"; volume: "14(1):2110. doi"; pages: " 
            10.1038/s41467-023-37914-x"
COMMENT     Sequence Label: pSLIK BirA* zeo
FEATURES             Location/Qualifiers
     source          1..12608
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1680..1913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2098..2142
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2440..2557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    2614..2638
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        2670..2984
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    2678..2696
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2714..2732
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2749..2767
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2785..2803
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2821..2839
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2856..2874
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2892..2910
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2957..2981
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             join(2991..3341,3342..3344,3345..3953)
                     /codon_start=1
                     /product="promiscuous R118G mutant of the E. coli biotin 
                     protein ligase (Cronan, 2005)"
                     /label=BirA(R118G)
                     /note="also known as BirA* or BioID"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRD
                     WGVDVFTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSG
                     DACIAEYQQAGRGGRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRK
                     LGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQG
                     WITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIG
                     DKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     protein_bind    complement(3959..3983)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        4033..5244
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     5081..5100
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             join(5303..5920,5921..5926,5927..5962,5963..5965,5966..6001,
                     6002..6004,6005..6040,6041..6049)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    6087..6660
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6254..6271)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6481..6500
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     promoter        6682..6729
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6748..7122
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     misc_feature    7296..7884
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7349..7369)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7767..7778)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7887..7903)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7888..7904)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             8413..8593
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8619..8636)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8625..8849
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8895..9323
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8982..9001)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9192..9213
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(9332..9352)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9337..9666
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9517..9652
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9579..9598
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        9714..9761
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             9780..10154
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    10284..10405
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10321..10340)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10375..10394
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(10454..10470)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10454..10470)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10467..10489)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10478..10494
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10502..10508,10509..10526,10527..10532))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10547..10568
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10685..10702)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10856..11444)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10936..10955)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11615..12406,12407..12475))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12238..12257
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12476..12580)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaattc tagacagtta tcaacaagtt tgtacaaaaa agcaggcttt
     2641 aaaggaacca attcagtcga ctggatcccg agtttactcc ctatcagtga tagagaacgt
     2701 atgtcgagtt tactccctat cagtgataga gaacgatgtc gagtttactc cctatcagtg
     2761 atagagaacg tatgtcgagt ttactcccta tcagtgatag agaacgtatg tcgagtttac
     2821 tccctatcag tgatagagaa cgtatgtcga gtttatccct atcagtgata gagaacgtat
     2881 gtcgagttta ctccctatca gtgatagaga acgtatgtcg aggtaggcgt gtacggtggg
     2941 aggcctatat aagcagagct cgtttagtga accgtcagat cgccactaga atgaaggaca
     3001 acaccgtgcc cctgaagctg atcgccctgc tggccaacgg cgagttccac tctggcgagc
     3061 agctgggaga gaccctggga atgagcagag ccgccatcaa caagcacatc cagacactga
     3121 gagactgggg agtggacgtg ttcaccgtgc ctggcaaggg ctacagcctg cctgagccta
     3181 tccagctgct gaacgccaag cagatcctgg gacagctgga tggcggaagc gtggccgtgc
     3241 tgcctgtgat cgactccacc aatcagtacc tgctggacag aatcggagag ctgaagtccg
     3301 gcgacgcctg catcgccgag taccagcagg ctggcagagg aggcagagga cggaagtggt
     3361 tcagcccatt cggagccaac ctgtacctgt ccatgttctg gagactggag cagggacctg
     3421 ctgctgccat cggactgagt ctggtgatcg gaatcgtgat ggccgaggtg ctgagaaagc
     3481 tgggagccga caaggtgaga gtgaagtggc ctaatgacct gtacctccag gaccgcaagc
     3541 tggctggcat cctggtggag ctgacaggca agacaggcga tgccgctcag atcgtgatcg
     3601 gagccggaat caacatggcc atgagaagag tggaggagag cgtggtgaac cagggctgga
     3661 tcaccctgca ggaggctggc atcaacctgg accggaacac cctggccgcc atgctgatca
     3721 gagagctgag agccgctctg gagctgttcg agcaggaggg actggctcct tacctgagca
     3781 gatgggagaa gctggacaac ttcatcaaca gacctgtgaa gctgatcatc ggcgacaagg
     3841 aaatcttcgg catctccaga ggaatcgaca agcagggagc tctgctgctg gagcaggacg
     3901 gaatcatcaa gccctggatg ggcggagaaa tctccctgag aagcgcagag aagactagac
     3961 ccagctttct tgtacaaagt ggttgataac atctcgagcc ataaagatgg ttaattaacc
     4021 cacccaagat ctggcctccg cgccgggttt tggcgcctcc cgcgggcgcc cccctcctca
     4081 cggcgagcgc tgccacgtca gacgaagggc gcagcgagcg tcctgatcct tccgcccgga
     4141 cgctcaggac agcggcccgc tgctcataag actcggcctt agaaccccag tatcagcaga
     4201 aggacatttt aggacgggac ttgggtgact ctagggcact ggttttcttt ccagagagcg
     4261 gaacaggcga ggaaaagtag tcccttctcg gcgattctgc ggagggatct ccgtggggcg
     4321 gtgaacgccg atgattatat aaggacgcgc cgggtgtggc acagctagtt ccgtcgcagc
     4381 cgggatttgg gtcgcggttc ttgtttgtgg atcgctgtga tcgtcacttg gtgagtagcg
     4441 ggctgctggg ctggccgggg ctttcgtggc cgccgggccg ctcggtggga cggaagcgtg
     4501 tggagagacc gccaagggct gtagtctggg tccgcgagca aggttgccct gaactggggg
     4561 ttggggggag cgcagcaaaa tggcggctgt tcccgagtct tgaatggaag acgcttgtga
     4621 ggcgggctgt gaggtcgttg aaacaaggtg gggggcatgg tgggcggcaa gaacccaagg
     4681 tcttgaggcc ttcgctaatg cgggaaagct cttattcggg tgagatgggc tggggcacca
     4741 tctggggacc ctgacgtgaa gtttgtcact gactggagaa ctcggtttgt cgtctgttgc
     4801 gggggcggca gttatggcgg tgccgttggg cagtgcaccc gtacctttgg gagcgcgcgc
     4861 cctcgtcgtg tcgtgacgtc acccgttctg ttggcttata atgcagggtg gggccacctg
     4921 ccggtaggtg tgcggtaggc ttttctccgt cgcaggacgc agggttcggg cctagggtag
     4981 gctctcctga atcgacaggc gccggacctc tggtgagggg agggataagt gaggcgtcag
     5041 tttctttggt cggttttatg tacctatctt cttaagtagc tgaagctccg gttttgaact
     5101 atgcgctcgg ggttggcgag tgtgttttgt gaagtttttt aggcaccttt tgaaatgtaa
     5161 tcatttgggt caatatgtaa ttttcagtgt tagactagta aattgtccgc taaattctgg
     5221 ccgtttttgg cttttttgtt agacgaagct tggtaccgag ctcggatctc caccccgtac
     5281 cggtcctgca gtcgaattca ccatgtctag actggacaag agcaaagtca taaacggagc
     5341 tctggaatta ctcaatggtg tcggtatcga aggcctgacg acaaggaaac tcgctcaaaa
     5401 gctgggagtt gagcagccta ccctgtactg gcacgtgaag aacaagcggg ccctgctcga
     5461 tgccctgcca atcgagatgc tggacaggca tcatacccac ttctgccccc tggaaggcga
     5521 gtcatggcaa gactttctgc ggaacaacgc caagtcatac cgctgtgctc tcctctcaca
     5581 tcgcgacggg gctaaagtgc atctcggcac ccgcccaaca gagaaacagt acgaaaccct
     5641 ggaaaatcag ctcgcgttcc tgtgtcagca aggcttctcc ctggagaacg cactgtacgc
     5701 tctgtccgcc gtgggccact ttacactggg ctgcgtattg gaggaacagg agcatcaagt
     5761 agcaaaagag gaaagagaga cacctaccac cgattctatg cccccacttc tgagacaagc
     5821 aattgagctg ttcgaccggc agggagccga acctgccttc cttttcggcc tggaactaat
     5881 catatgtggc ctggagaaac agctaaagtg cgaaagcggc gggccgaccg acgcccttga
     5941 cgattttgac ttagacatgc tcccagccga tgcccttgac gactttgacc ttgatatgct
     6001 gcctgctgac gctcttgacg attttgacct tgacatgctc cccgggtaac taagtaagga
     6061 tccgcggccg cactagagga attccgcccc tctcccyccc ccccccctaa cgttactggc
     6121 cgaagccgct tggaataagg ccggtgtgcg tttgtctata tgttattttc caccatattg
     6181 ccgtcttttg gcaatgtgag ggcccggaaa cctggccctg tcttcttgac gagcattcct
     6241 aggggtcttt cccctctcgc caaaggaatg caaggtctgt tgaatgtcgt gaaggaagca
     6301 gttcctctgg aagcttcttg aagacaaaca acgtctgtag cgaccctttg caggcagcgg
     6361 aaccccccac ctggcgacag gtgcctctgc ggccaaaagc cacgtgtata agatacacct
     6421 gcaaaggcgg cacaacccca gtgccacgtt gtgagttgga tagttgtgga aagagtcaaa
     6481 tggctctcct caagcgtatt caacaagggg ctgaaggatg cccagaaggt accccattgt
     6541 atgggatctg atctggggcc tcggtgcaca tgctttacat gtgtttagtc gaggttaaaa
     6601 aaacgtctag gccccccgaa ccacggggac gtggttttcc tttgaaaaac acgatgataa
     6661 gcttaccggt acgcgtgatg tgttgacaat taatcatcgg catagtatat cggcatagta
     6721 taatacgaca aggtgaggaa ctaaaccatg gccaagttga ccagtgccgt tccggtgctc
     6781 accgcgcgcg acgtcgccgg agcggtcgag ttctggaccg accggctcgg gttctcccgg
     6841 gacttcgtgg aggacgactt cgccggtgtg gtccgggacg acgtgaccct gttcatcagc
     6901 gcggtccagg accaggtggt gccggacaac accctggcct gggtgtgggt gcgcggcctg
     6961 gacgagctgt acgccgagtg gtcggaggtc gtgtccacga acttccggga cgcctccggg
     7021 ccggccatga ccgagatcgg cgagcagccg tgggggcggg agttcgccct gcgcgacccg
     7081 gccggcaact gcgtgcactt cgtggccgag gagcaggact gacacatctg tacaagtaaa
     7141 gcggccgcga ctctagatca taatcagcca taccacattt gtagaggttt tacttgcttt
     7201 aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt
     7261 ttagtccctc ccaattcgat atcaagctta tcgataatca acctctggat tacaaaattt
     7321 gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg
     7381 ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt
     7441 ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg
     7501 tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc
     7561 agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg
     7621 cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt
     7681 tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg tgttgccacc tggattctgc
     7741 gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg
     7801 gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga
     7861 tctccctttg ggccgcctcc ccgcatcgat accgtcgacc tcgatcgaga cctagaaaaa
     7921 catggagcaa tcacaagtag caatacagca gctaccaatg ctgattgtgc ctggctagaa
     7981 gcacaagagg aggaggaggt gggttttcca gtcacacctc aggtaccttt aagaccaatg
     8041 acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg
     8101 ctaattcact cccaacgaag acaagatatc cttgatctgt ggatctacca cacacaaggc
     8161 tacttccctg attggcagaa ctacacacca gggccaggga tcagatatcc actgaccttt
     8221 ggatggtgct acaagctagt accagttgag caagagaagg tagaagaagc caatgaagga
     8281 gagaacaccc gcttgttaca ccctgtgagc ctgcatggga tggatgaccc ggagagagaa
     8341 gtattagagt ggaggtttga cagccgccta gcatttcatc acatggcccg agagctgcat
     8401 ccggactgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
     8461 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
     8521 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
     8581 aaaatctcta gcagggcccg tttaaacccg ctgatcagcc tcgactgtgc cttctagttg
     8641 ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc
     8701 cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc
     8761 tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag
     8821 gcatgctggg gatgcggtgg gctctatggc ttctgaggcg gaaagaacca gctggggctc
     8881 tagggggtat ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac
     8941 gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc
     9001 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt
     9061 agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg
     9121 ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac
     9181 gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta
     9241 ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat
     9301 ttaacaaaaa tttaacgcga attaattctg tggaatgtgt gtcagttagg gtgtggaaag
     9361 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
     9421 aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
     9481 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
     9541 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
     9601 gcctctgcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt
     9661 tgcaaaaagc tcccgggagc ttgtatatcc attttcggat ctgatcagca cgtgttgaca
     9721 attaatcatc ggcatagtat atcggcatag tataatacga caaggtgagg aactaaacca
     9781 tggccaagtt gaccagtgcc gttccggtgc tcaccgcgcg cgacgtcgcc ggagcggtcg
     9841 agttctggac cgaccggctc gggttctccc gggacttcgt ggaggacgac ttcgccggtg
     9901 tggtccggga cgacgtgacc ctgttcatca gcgcggtcca ggaccaggtg gtgccggaca
     9961 acaccctggc ctgggtgtgg gtgcgcggcc tggacgagct gtacgccgag tggtcggagg
    10021 tcgtgtccac gaacttccgg gacgcctccg ggccggccat gaccgagatc ggcgagcagc
    10081 cgtgggggcg ggagttcgcc ctgcgcgacc cggccggcaa ctgcgtgcac ttcgtggccg
    10141 aggagcagga ctgacacgtg ctacgagatt tcgattccac cgccgccttc tatgaaaggt
    10201 tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca
    10261 tgctggagtt cttcgcccac cccaacttgt ttattgcagc ttataatggt tacaaataaa
    10321 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
    10381 tgtccaaact catcaatgta tcttatcatg tctgtatacc gtcgacctct agctagagct
    10441 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
    10501 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac
    10561 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
    10621 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg
    10681 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
    10741 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
    10801 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
    10861 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
    10921 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
    10981 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
    11041 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
    11101 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
    11161 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
    11221 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
    11281 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
    11341 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
    11401 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
    11461 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
    11521 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
    11581 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
    11641 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
    11701 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
    11761 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
    11821 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
    11881 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
    11941 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
    12001 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
    12061 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
    12121 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
    12181 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
    12241 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
    12301 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
    12361 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
    12421 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
    12481 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
    12541 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
    12601 cacctgac
//
