LOCUS       sequence-375848-       12725 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression vector for an inducible 3xFLAG-NUP37.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12725)
  AUTHORS   Wang L, Paudel BB, McKnight RA, Janes KA
  TITLE     Nucleocytoplasmic transport of active HER2 causes fractional escape 
            from the DCIS-like state.
  JOURNAL   Nat Commun. 2023 Apr 13;14(1):2110. doi: 10.1038/s41467-023-37914-x.
  PUBMED    37055441
REFERENCE   2  (bases 1 to 12725)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-04-13"; volume: "14(1):2110. doi"; pages: " 
            10.1038/s41467-023-37914-x"
COMMENT     Sequence Label: pSLIK 3xFLAG-NUP37 zeo
FEATURES             Location/Qualifiers
     source          1..12725
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1680..1913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2098..2142
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2440..2557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    2614..2638
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        2670..2984
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    2678..2696
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2714..2732
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2749..2767
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2785..2803
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2821..2839
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2856..2874
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2892..2910
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2957..2981
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             2994..3059
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     protein_bind    complement(4076..4100)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        4150..5361
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     5198..5217
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             join(5420..6037,6038..6043,6044..6079,6080..6082,6083..6118,
                     6119..6121,6122..6157,6158..6166)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    6204..6777
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6371..6388)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6598..6617
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     promoter        6799..6846
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6865..7239
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     misc_feature    7413..8001
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7466..7486)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7884..7895)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(8004..8020)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8005..8021)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             8530..8710
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8736..8753)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8742..8966
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      9012..9440
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9099..9118)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9309..9330
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(9449..9469)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9454..9783
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9634..9769
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9696..9715
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        9831..9878
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             9897..10271
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    10401..10522
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10438..10457)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10492..10511
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(10571..10587)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10571..10587)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10584..10606)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10595..10611
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10619..10625,10626..10643,10644..10649))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10664..10685
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10802..10819)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10973..11561)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11053..11072)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11732..12523,12524..12592))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12355..12374
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12593..12697)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaattc tagacagtta tcaacaagtt tgtacaaaaa agcaggcttt
     2641 aaaggaacca attcagtcga ctggatcccg agtttactcc ctatcagtga tagagaacgt
     2701 atgtcgagtt tactccctat cagtgataga gaacgatgtc gagtttactc cctatcagtg
     2761 atagagaacg tatgtcgagt ttactcccta tcagtgatag agaacgtatg tcgagtttac
     2821 tccctatcag tgatagagaa cgtatgtcga gtttatccct atcagtgata gagaacgtat
     2881 gtcgagttta ctccctatca gtgatagaga acgtatgtcg aggtaggcgt gtacggtggg
     2941 aggcctatat aagcagagct cgtttagtga accgtcagat cgccactagt atggactaca
     3001 aagaccatga cggtgattat aaagatcatg acatcgatta caaggatgac gatgacaaga
     3061 tgaagcaaga tgcctcaaga aatgctgcct acactgtgga ttgtgaagat tatgtgcatg
     3121 tggtagaatt taatcccttt gagaatgggg attcaggaaa cctaattgca tatggtggca
     3181 ataattatgt ggtcattggc acgtgtacgt ttcaggaaga agaagcagac gttgaaggca
     3241 ttcagtataa aacacttcga acatttcacc atggagtcag ggttgatggc atagcttgga
     3301 gcccagagac tagacttgat tcattgcctc cagtaatcaa attttgtact tcagctgctg
     3361 atatgaaaat tagattattt acttcagatc ttcaggataa aaatgaatat aaggttttag
     3421 agggccatac cgatttcatt aatggtttgg tgtttgatcc caaagaaggc caagaaattg
     3481 caagtgtgag tgacgatcac acctgcagga tttggaactt ggaaggagtg caaacagctc
     3541 attttgttct tcattctcct ggcatgagtg tgtgctggca tcctgaggag acttttaagc
     3601 taatggttgc agagaagaat ggaacaatcc ggttttatga tcttttggcc caacaggcta
     3661 ttttatctct tgaatcagaa caagtgccat taatgtcagc acactggtgc ttaaaaaaca
     3721 ccttcaaagt tggagccgtt gcaggaaatg attggttaat ttgggatatt actcggtcca
     3781 gttatcctca aaataagaga cctgttcaca tggatcgagc ctgcttattc aggtggtcca
     3841 caattagtga aaatctgttt gcaaccactg gttatcctgg caaaatggca agccagtttc
     3901 aaattcatca tttaggacac cctcagccca tcctcatggg ttctgtagcc gttggatctg
     3961 gactgtcctg gcatcgaact ctccctctgt gtgtaattgg aggagaccac aagctgttgt
     4021 tttgggtgac tgaagtataa caattggaag actaatgcgt tttcgagata tctagaccca
     4081 gctttcttgt acaaagtggt tgataacatc tcgagccata aagatggtta attaacccac
     4141 ccaagatctg gcctccgcgc cgggttttgg cgcctcccgc gggcgccccc ctcctcacgg
     4201 cgagcgctgc cacgtcagac gaagggcgca gcgagcgtcc tgatccttcc gcccggacgc
     4261 tcaggacagc ggcccgctgc tcataagact cggccttaga accccagtat cagcagaagg
     4321 acattttagg acgggacttg ggtgactcta gggcactggt tttctttcca gagagcggaa
     4381 caggcgagga aaagtagtcc cttctcggcg attctgcgga gggatctccg tggggcggtg
     4441 aacgccgatg attatataag gacgcgccgg gtgtggcaca gctagttccg tcgcagccgg
     4501 gatttgggtc gcggttcttg tttgtggatc gctgtgatcg tcacttggtg agtagcgggc
     4561 tgctgggctg gccggggctt tcgtggccgc cgggccgctc ggtgggacgg aagcgtgtgg
     4621 agagaccgcc aagggctgta gtctgggtcc gcgagcaagg ttgccctgaa ctgggggttg
     4681 gggggagcgc agcaaaatgg cggctgttcc cgagtcttga atggaagacg cttgtgaggc
     4741 gggctgtgag gtcgttgaaa caaggtgggg ggcatggtgg gcggcaagaa cccaaggtct
     4801 tgaggccttc gctaatgcgg gaaagctctt attcgggtga gatgggctgg ggcaccatct
     4861 ggggaccctg acgtgaagtt tgtcactgac tggagaactc ggtttgtcgt ctgttgcggg
     4921 ggcggcagtt atggcggtgc cgttgggcag tgcacccgta cctttgggag cgcgcgccct
     4981 cgtcgtgtcg tgacgtcacc cgttctgttg gcttataatg cagggtgggg ccacctgccg
     5041 gtaggtgtgc ggtaggcttt tctccgtcgc aggacgcagg gttcgggcct agggtaggct
     5101 ctcctgaatc gacaggcgcc ggacctctgg tgaggggagg gataagtgag gcgtcagttt
     5161 ctttggtcgg ttttatgtac ctatcttctt aagtagctga agctccggtt ttgaactatg
     5221 cgctcggggt tggcgagtgt gttttgtgaa gttttttagg caccttttga aatgtaatca
     5281 tttgggtcaa tatgtaattt tcagtgttag actagtaaat tgtccgctaa attctggccg
     5341 tttttggctt ttttgttaga cgaagcttgg taccgagctc ggatctccac cccgtaccgg
     5401 tcctgcagtc gaattcacca tgtctagact ggacaagagc aaagtcataa acggagctct
     5461 ggaattactc aatggtgtcg gtatcgaagg cctgacgaca aggaaactcg ctcaaaagct
     5521 gggagttgag cagcctaccc tgtactggca cgtgaagaac aagcgggccc tgctcgatgc
     5581 cctgccaatc gagatgctgg acaggcatca tacccacttc tgccccctgg aaggcgagtc
     5641 atggcaagac tttctgcgga acaacgccaa gtcataccgc tgtgctctcc tctcacatcg
     5701 cgacggggct aaagtgcatc tcggcacccg cccaacagag aaacagtacg aaaccctgga
     5761 aaatcagctc gcgttcctgt gtcagcaagg cttctccctg gagaacgcac tgtacgctct
     5821 gtccgccgtg ggccacttta cactgggctg cgtattggag gaacaggagc atcaagtagc
     5881 aaaagaggaa agagagacac ctaccaccga ttctatgccc ccacttctga gacaagcaat
     5941 tgagctgttc gaccggcagg gagccgaacc tgccttcctt ttcggcctgg aactaatcat
     6001 atgtggcctg gagaaacagc taaagtgcga aagcggcggg ccgaccgacg cccttgacga
     6061 ttttgactta gacatgctcc cagccgatgc ccttgacgac tttgaccttg atatgctgcc
     6121 tgctgacgct cttgacgatt ttgaccttga catgctcccc gggtaactaa gtaaggatcc
     6181 gcggccgcac tagaggaatt ccgcccctct cccycccccc cccctaacgt tactggccga
     6241 agccgcttgg aataaggccg gtgtgcgttt gtctatatgt tattttccac catattgccg
     6301 tcttttggca atgtgagggc ccggaaacct ggccctgtct tcttgacgag cattcctagg
     6361 ggtctttccc ctctcgccaa aggaatgcaa ggtctgttga atgtcgtgaa ggaagcagtt
     6421 cctctggaag cttcttgaag acaaacaacg tctgtagcga ccctttgcag gcagcggaac
     6481 cccccacctg gcgacaggtg cctctgcggc caaaagccac gtgtataaga tacacctgca
     6541 aaggcggcac aaccccagtg ccacgttgtg agttggatag ttgtggaaag agtcaaatgg
     6601 ctctcctcaa gcgtattcaa caaggggctg aaggatgccc agaaggtacc ccattgtatg
     6661 ggatctgatc tggggcctcg gtgcacatgc tttacatgtg tttagtcgag gttaaaaaaa
     6721 cgtctaggcc ccccgaacca cggggacgtg gttttccttt gaaaaacacg atgataagct
     6781 taccggtacg cgtgatgtgt tgacaattaa tcatcggcat agtatatcgg catagtataa
     6841 tacgacaagg tgaggaacta aaccatggcc aagttgacca gtgccgttcc ggtgctcacc
     6901 gcgcgcgacg tcgccggagc ggtcgagttc tggaccgacc ggctcgggtt ctcccgggac
     6961 ttcgtggagg acgacttcgc cggtgtggtc cgggacgacg tgaccctgtt catcagcgcg
     7021 gtccaggacc aggtggtgcc ggacaacacc ctggcctggg tgtgggtgcg cggcctggac
     7081 gagctgtacg ccgagtggtc ggaggtcgtg tccacgaact tccgggacgc ctccgggccg
     7141 gccatgaccg agatcggcga gcagccgtgg gggcgggagt tcgccctgcg cgacccggcc
     7201 ggcaactgcg tgcacttcgt ggccgaggag caggactgac acatctgtac aagtaaagcg
     7261 gccgcgactc tagatcataa tcagccatac cacatttgta gaggttttac ttgctttaaa
     7321 aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttta
     7381 gtccctccca attcgatatc aagcttatcg ataatcaacc tctggattac aaaatttgtg
     7441 aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt
     7501 taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata
     7561 aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg
     7621 tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc
     7681 tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct
     7741 gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt
     7801 cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg
     7861 ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc
     7921 tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct
     7981 ccctttgggc cgcctccccg catcgatacc gtcgacctcg atcgagacct agaaaaacat
     8041 ggagcaatca caagtagcaa tacagcagct accaatgctg attgtgcctg gctagaagca
     8101 caagaggagg aggaggtggg ttttccagtc acacctcagg tacctttaag accaatgact
     8161 tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta
     8221 attcactccc aacgaagaca agatatcctt gatctgtgga tctaccacac acaaggctac
     8281 ttccctgatt ggcagaacta cacaccaggg ccagggatca gatatccact gacctttgga
     8341 tggtgctaca agctagtacc agttgagcaa gagaaggtag aagaagccaa tgaaggagag
     8401 aacacccgct tgttacaccc tgtgagcctg catgggatgg atgacccgga gagagaagta
     8461 ttagagtgga ggtttgacag ccgcctagca tttcatcaca tggcccgaga gctgcatccg
     8521 gactgtactg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta
     8581 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc
     8641 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa
     8701 atctctagca gggcccgttt aaacccgctg atcagcctcg actgtgcctt ctagttgcca
     8761 gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac
     8821 tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat
     8881 tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca
     8941 tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctctag
     9001 ggggtatccc cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
     9061 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
     9121 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
     9181 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
     9241 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
     9301 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
     9361 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
     9421 acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc
     9481 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg
     9541 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
     9601 tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc
     9661 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
     9721 tctgcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc
     9781 aaaaagctcc cgggagcttg tatatccatt ttcggatctg atcagcacgt gttgacaatt
     9841 aatcatcggc atagtatatc ggcatagtat aatacgacaa ggtgaggaac taaaccatgg
     9901 ccaagttgac cagtgccgtt ccggtgctca ccgcgcgcga cgtcgccgga gcggtcgagt
     9961 tctggaccga ccggctcggg ttctcccggg acttcgtgga ggacgacttc gccggtgtgg
    10021 tccgggacga cgtgaccctg ttcatcagcg cggtccagga ccaggtggtg ccggacaaca
    10081 ccctggcctg ggtgtgggtg cgcggcctgg acgagctgta cgccgagtgg tcggaggtcg
    10141 tgtccacgaa cttccgggac gcctccgggc cggccatgac cgagatcggc gagcagccgt
    10201 gggggcggga gttcgccctg cgcgacccgg ccggcaactg cgtgcacttc gtggccgagg
    10261 agcaggactg acacgtgcta cgagatttcg attccaccgc cgccttctat gaaaggttgg
    10321 gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc
    10381 tggagttctt cgcccacccc aacttgttta ttgcagctta taatggttac aaataaagca
    10441 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
    10501 ccaaactcat caatgtatct tatcatgtct gtataccgtc gacctctagc tagagcttgg
    10561 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
    10621 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
    10681 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
    10741 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
    10801 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
    10861 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
    10921 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
    10981 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
    11041 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
    11101 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
    11161 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
    11221 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
    11281 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
    11341 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
    11401 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
    11461 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
    11521 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
    11581 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
    11641 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
    11701 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
    11761 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
    11821 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
    11881 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
    11941 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
    12001 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
    12061 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
    12121 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
    12181 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
    12241 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
    12301 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
    12361 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
    12421 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
    12481 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
    12541 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
    12601 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
    12661 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
    12721 ctgac
//
