LOCUS       sequence-37585-e         784 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 784)
  AUTHORS   Schnutgen F, Ehrmann F, Poser I, Hubner NC, Hansen J, Floss T, 
            Devries I, Wurst W, Hyman A, Mann M, von Melchner H
  TITLE     Resources for proteomics in mouse embryonic stem cells.
  JOURNAL   Nat Methods. 2011 Feb . 8(2):103-4.
  PUBMED    21278719
REFERENCE   2  (bases 1 to 784)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2011 Feb . 8(2):103-4."
COMMENT     Sequence Label: pDTnLAPP2A frame 2
FEATURES             Location/Qualifiers
     source          1..784
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    218..251
                     /label=FRT3
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision with other FRT3 sites
                     (Bosch et al., 2015)"
     protein_bind    270..339
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     CDS             complement(557..580)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(596..616)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             complement(629..673)
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     CDS             complement(713..736)
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
ORIGIN
        1 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
       61 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
      121 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
      181 tttcaatggc cggatccgtt gctgaagttc ctattccgaa gttcctattc ttcaaatagt
      241 ataggaactt cgttatgcag cgtggaccag atgggtgagg tggagtacgc gcccggggag
      301 cccaagggca cgccctggca cccgcaccgc ggcttcgaga ccgtcacgaa tgcatgatat
      361 ccgcggccac cctcaaaggc atcaccgcgg gccaggtgaa tatcaaatcc tcctcgtttt
      421 tggaaactga caatcttagc gcagaagtca tgcccgcttt tgagagggag tactcacccc
      481 gaacagctgg ccctcgcaga cagcgatgcg gtagagagtg aggacgaacg cgggccctgg
      541 gcaggtcgtc ggtcagcttg tcgtcgtcgt ccttatagtc ccctccgcct ccgcctccct
      601 gaaagtacag gttctcgcct cctcctccgc tgtccatgtg ctgccgctcg aatttagcag
      661 cagctgtttc tttgaacttg gcggcgctgc cagaggggcc agagctgctc agggggccct
      721 ggaacagcac ttccaggctg ctgctgccgc tggggccgct gctcttgtac agctcgtcca
      781 tgcc
//
