LOCUS       sequence-375852-        8441 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Retroviral expression vector for NGF-ERBB2 -2xFKBP-HA construct.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8441)
  AUTHORS   Wang L, Paudel BB, McKnight RA, Janes KA
  TITLE     Nucleocytoplasmic transport of active HER2 causes fractional escape 
            from the DCIS-like state.
  JOURNAL   Nat Commun. 2023 Apr 13;14(1):2110. doi: 10.1038/s41467-023-37914-x.
  PUBMED    37055441
REFERENCE   2  (bases 1 to 8441)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-04-13"; volume: "14(1):2110. doi"; pages: " 
            10.1038/s41467-023-37914-x"
COMMENT     Sequence Label: pBabe NGF-ERBB2-2xFKBP-HA hygro
FEATURES             Location/Qualifiers
     source          1..8441
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..470
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    535..734
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             935..1351
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1281..1303
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1319..1335
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     CDS             3936..4256
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
     CDS             4263..4583
                     /codon_start=1
                     /product="F36V mutant of FK506-binding protein FKBP12"
                     /label=DmrB
                     /note="binds synthetic ligands that do not bind wild-type 
                     FKBP"
                     /translation="GVQVETTSPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
     CDS             4590..4616
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(4631..4651)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4636..4965
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4816..4951
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5039..6064
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             5039..6064
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     primer_bind     6581..6600
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     LTR             6862..7331
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     CDS             complement(join(7446..8237,8238..8306))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8069..8088
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8307..8411)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctgaatatgg gccaaacagg atatctgtgg taagcagttc ctgccccggc tcagggccaa
       61 gaacagatgg aacagctgaa tatgggccaa acaggatatc tgtggtaagc agttcctgcc
      121 ccggctcagg gccaagaaca gatggtcccc agatgcggtc cagccctcag cagtttctag
      181 agaaccatca gatgtttcca gggtgcccca aggacctgaa atgaccctgt gccttatttg
      241 aactaaccaa tcagttcgct tctcgcttct gttcgcgcgc ttctgctccc cgagctcaat
      301 aaaagagccc acaacccctc actcggggcg ccagtcctcc gattgactga gtcgcccggg
      361 tacccgtgta tccaataaac cctcttgcag ttgcatccga cttgtggtct cgctgttcct
      421 tgggagggtc tcctctgagt gattgactac ccgtcagcgg gggtctttca tttgggggct
      481 cgtccgggat cgggagaccc ctgcccaggg accaccgacc caccaccggg aggcaagctg
      541 gccagcaact tatctgtgtc tgtccgattg tctagtgtct atgactgatt ttatgcgcct
      601 gcgtcggtac tagttagcta actagctctg tatctggcgg acccgtggtg gaactgacga
      661 gttctgaaca cccggccgca accctgggag acgtcccagg gactttgggg gccgtttttg
      721 tggcccgacc tgaggaaggg agtcgatgtg gaatccgacc ccgtcaggat atgtggttct
      781 ggtaggagac gagaacctaa aacagttccc gcctccgtct gaatttttgc tttcggtttg
      841 gaaccgaagc cgcgcgtctt gtctgctgca gcgctgcagc atcgttctgt gttgtctctg
      901 tctgactgtg tttctgtatt tgtctgaaaa ttagggccag actgttacca ctcccttaag
      961 tttgacctta ggtcactgga aagatgtcga gcggatcgct cacaaccagt cggtagatgt
     1021 caagaagaga cgttgggtta ccttctgctc tgcagaatgg ccaaccttta acgtcggatg
     1081 gccgcgagac ggcaccttta accgagacct catcacccag gttaagatca aggtcttttc
     1141 acctggcccg catggacacc cagaccaggt cccctacatc gtgacctggg aagccttggc
     1201 ttttgacccc cctccctggg tcaagccctt tgtacaccct aagcctccgc ctcctcttcc
     1261 tccatccgcc ccgtctctcc cccttgaacc tcctcgttcg accccgcctc gatcctccct
     1321 ttatccagcc ctcactcctt ctctaggcgc cggccggatc catgggggca ggtgccaccg
     1381 gccgcgccat ggacgggccg cgcctgctgc tgttgctgct tctgggggtg tcccttggag
     1441 gtgccaagga ggcatgcccc acaggcctgt acacacacag cggtgagtgc tgcaaagcct
     1501 gcaacctggg cgagggtgtg gcccagccct gtggagccaa ccagaccgtg tgtgagccct
     1561 gcctggacag cgtgacgttc tccgacgtgg tgagcgcgac cgagccgtgc aagccgtgca
     1621 ccgagtgcgt ggggctccag agcatgtcgg cgccgtgcgt ggaggccgac gacgccgtgt
     1681 gccgctgcgc ctacggctac taccaggatg agacgactgg gcgctgcgag gcgtgccgcg
     1741 tgtgcgaggc gggctcgggc ctcgtgttct cctgccagga caagcagaac accgtgtgcg
     1801 aggagtgccc cgacggcacg tattccgacg aggccaacca cgtggacccg tgcctgccct
     1861 gcaccgtgtg cgaggacacc gagcgccagc tccgcgagtg cacacgctgg gccgacgccg
     1921 agtgcgagga gatccctggc cgttggatta cacggtccac acccccagag ggctcggaca
     1981 gcacagcccc cagcacccag gagcctgagg cacctccaga acaagacctc atagccagca
     2041 cggtggcagg tgtggtgacc acagtgatgg gcagctccca gcccgtggtg acccgaggca
     2101 ccaccgacaa cctcatccct gtctattgct ccatcctggc tgctgtggtt gtgggtcttg
     2161 tggcctacat agccttcaag aggtctagaa agcgacggca gcagaagatc cggaagtaca
     2221 cgatgcggag actgctgcag gaaacggagc tggtggagcc gctgacacct agcggagcga
     2281 tgcccaacca ggcgcagatg cggatcctga aagagacgga gctgaggaag gtgaaggtgc
     2341 ttggatctgg cgcttttggc acagtctaca agggcatctg gatccctgat ggggagaatg
     2401 tgaaaattcc agtggccatc aaagtgttga gggaaaacac atcccccaaa gccaacaaag
     2461 aaatcttaga cgaagcatac gtgatggctg gtgtgggctc cccatatgtc tcccgccttc
     2521 tgggcatctg cctgacatcc acggtgcagc tggtgacaca gcttatgccc tatggctgcc
     2581 tcttagacca tgtccgggaa aaccgcggac gcctgggctc ccaggacctg ctgaactggt
     2641 gtatgcagat tgccaagggg atgagctacc tggaggatgt gcggctcgta cacagggact
     2701 tggccgctcg gaacgtgctg gtcaagagtc ccaaccatgt caaaattaca gacttcgggc
     2761 tggctcggct gctggacatt gacgagacag agtaccatgc agatgggggc aaggtgccca
     2821 tcaagtggat ggcgctggag tccattctcc gccggcggtt cacccaccag agtgatgtgt
     2881 ggagttatgg tgtgactgtg tgggagctga tgacttttgg ggccaaacct tacgatggga
     2941 tcccagcccg ggagatccct gacctgctgg aaaaggggga gcggctgccc cagcccccca
     3001 tctgcaccat tgatgtctac atgatcatgg tcaaatgttg gatgattgac tctgaatgtc
     3061 ggccaagatt ccgggagttg gtgtctgaat tctcccgcat ggccagggac ccccagcgct
     3121 ttgtggtcat ccagaatgag gacttgggcc cagccagtcc cttggacagc accttctacc
     3181 gctcactgct ggaggacgat gacatggggg acctggtgga tgctgaggag tatctggtac
     3241 cccagcaggg cttcttctgt ccagaccctg ccccgggcgc tgggggcatg gtccaccaca
     3301 ggcaccgcag ctcatctacc aggagtggcg gtggggacct gacactaggg ctggagccct
     3361 ctgaagagga ggcccccagg tctccactgg caccctccga aggggctggc tccgatgtat
     3421 ttgatggtga cctgggaatg ggggcagcca aggggctgca aagcctcccc acacatgacc
     3481 ccagccctct acagcggtac agtgaggacc ccacagtacc cctgccctct gagactgatg
     3541 gctacgttgc ccccctgacc tgcagccccc agcctgaata tgtgaaccag ccagatgttc
     3601 ggccccagcc cccttcgccc cgagagggcc ctctgcctgc tgcccgacct gctggtgcca
     3661 ctctggaaag gcccaagact ctctccccag ggaagaatgg ggtcgtcaaa gacgtttttg
     3721 cctttggggg tgccgtggag aaccccgagt acttgacacc ccagggagga gctgcccctc
     3781 agccccaccc tcctcctgcc ttcagcccag ccttcgacaa cctctattac tgggaccagg
     3841 acccaccaga gcggggggct ccacccagca ccttcaaagg gacacctacg gcagagaacc
     3901 cagagtacct gggtctggac gtgccagtgc aattgggagt gcaggtggaa accatctccc
     3961 cgggagacgg gcgcaccttc cccaagcgcg gtcagacctg cgtggtgcac tacaccggga
     4021 tgcttgaaga tggaaagaaa tttgattcct cccgggacag aaacaagccc tttaagttta
     4081 tgctaggcaa gcaggaggtg atccgaggct gggaagaagg ggttgcccag atgagtgtgg
     4141 gtcagagagc caaactgact atatctccag attatgccta tggtgccact gggcacccag
     4201 gcatcatccc accacatgcc actctcgtct tcgatgtgga gcttctaaaa ctggaagtcg
     4261 agggcgtgca ggtggagact acctccccag gagacgggcg caccttcccc aagcgcggcc
     4321 agacctgcgt ggtgcactac accgggatgc ttgaagatgg aaagaaattt gattcctccc
     4381 gggacagaaa caagcccttt aagtttatgc taggcaagca ggaggtgatc cgaggctggg
     4441 aagaaggggt tgcccagatg agtgtgggtc agagagccaa actgactata tctccagatt
     4501 atgcctatgg tgccactggg cacccaggca tcatcccacc acatgccact ctcgtcttcg
     4561 atgtggagct tctaaaactg gaaactagtt atccgtacga cgtaccagac tacgcataag
     4621 tcgaccctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc
     4681 agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc
     4741 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg
     4801 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
     4861 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc
     4921 cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct tgccagctgg
     4981 ggcgccctct ggtaaggttg ggaagccctg cagatctgat cccggggggc aatgagatat
     5041 gaaaaagcct gaactcaccg cgacgtctgt cgagaagttt ctgatcgaaa agttcgacag
     5101 cgtctccgac ctgatgcagc tctcggaggg cgaagaatct cgtgctttca gcttcgatgt
     5161 aggagggcgt ggatatgtcc tgcgggtaaa tagctgcgcc gatggtttct acaaagatcg
     5221 ttatgtttat cggcactttg catcggccgc gctcccgatt ccggaagtgc ttgacattgg
     5281 ggaatttagc gagagcctga cctattgcat ctcccgccgt gcacagggtg tcacgttgca
     5341 agacctgcct gaaaccgaac tgcccgctgt tctgcagccg gtcgcggagg ccatggatgc
     5401 gatcgctgcg gccgatctta gccagacgag cgggttcggc ccattcggac cgcaaggaat
     5461 cggtcaatac actacatggc gtgatttcat atgcgcgatt gctgatcccc atgtgtatca
     5521 ctggcaaact gtgatggacg acaccgtcag tgcgtccgtc gcgcaggctc tcgatgagct
     5581 gatgctttgg gccgaggact gccccgaagt ccggcacctc gtgcacgcgg atttcggctc
     5641 caacaatgtc ctgacggaca atggccgcat aacagcggtc attgactgga gcgaggcgat
     5701 gttcggggat tcccaatacg aggtcgccaa catcttcttc tggaggccgt ggttggcttg
     5761 tatggagcag cagacgcgct acttcgagcg gaggcatccg gagcttgcag gatcgccgcg
     5821 gctccgggcg tatatgctcc gcattggtct tgaccaactc tatcagagct tggttgacgg
     5881 caatttcgat gatgcagctt gggcgcaggg tcgatgcgac gcaatcgtcc gatccggagc
     5941 cgggactgtc gggcgtacac aaatcgcccg cagaagcgcg gccgtctgga ccgatggctg
     6001 tgtagaagta ctcgccgata gtggaaaccg acgccccagc actcgtccga gggcaaagga
     6061 atagagtaga tgccgaccgg gatctatcga tgcggcgggt gtggtggtta cgcgtaatct
     6121 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     6181 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     6241 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     6301 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     6361 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     6421 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     6481 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     6541 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     6601 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     6661 gggggcggag cctatggaaa aacgccagca acgcatcgat aaaataaaag attttattta
     6721 gtctccagaa aaagggggga atgaaagacc ccacctgtag gtttggcaag ctagcttaag
     6781 taacgccatt ttgcaaggca tggaaaaata cataactgag aatagagaag ttcagatcaa
     6841 ggtcaggaac agatggaaca gctgaatatg ggccaaacag gatatctgtg gtaagcagtt
     6901 cctgccccgg ctcagggcca agaacagatg gaacagctga atatgggcca aacaggatat
     6961 ctgtggtaag cagttcctgc cccggctcag ggccaagaac agatggtccc cagatgcggt
     7021 ccagccctca gcagtttcta gagaaccatc agatgtttcc agggtgcccc aaggacctga
     7081 aatgaccctg tgccttattt gaactaacca atcagttcgc ttctcgcttc tgttcgcgcg
     7141 cttctgctcc ccgagctcaa taaaagagcc cacaacccct cactcggggc gccagtcctc
     7201 cgattgactg agtcgcccgg gtacccgtgt atccaataaa ccctcttgca gttgcatccg
     7261 acttgtggtc tcgctgttcc ttgggagggt ctcctctgag tgattgacta cccgtcagcg
     7321 ggggtctttc acatgcagca tgtatcaaaa ttaatttggt tttttttctt aagtatttac
     7381 attaaatggc catagtgcgg ccgcaaatca atctaaagta tatatgagta aacttggtct
     7441 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     7501 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     7561 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     7621 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     7681 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     7741 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     7801 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     7861 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     7921 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     7981 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     8041 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     8101 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     8161 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     8221 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     8281 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     8341 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     8401 ggggttccgc gcacatttcc ccgaaaagtg ccacctgcag c
//
