LOCUS       sequence-375855-        9017 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  E. coli - Eggerthella lenta shuttle plasmid (TetR), dadR(linker 
            region shuffled) complementation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9017)
  AUTHORS   Dong X, Guthrie BGH, Alexander M, Noecker C, Ramirez L, Glasser NR, 
            Turnbaugh PJ, Balskus EP
  TITLE     Genetic manipulation of the human gut bacterium Eggerthella lenta 
            reveals a widespread family of transcriptional regulators.
  JOURNAL   Nat Commun. 2022 Dec 9;13(1):7624. doi: 10.1038/s41467-022-33576-3.
  PUBMED    36494336
REFERENCE   2  (bases 1 to 9017)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-12-9"; volume: "13(1):7624. doi"; pages: " 
            10.1038/s41467-022-33576-3"
COMMENT     Sequence Label: pXD70Tet(DadRmt4)-DadR(linker)
FEATURES             Location/Qualifiers
     source          1..9017
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(4990..5017)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     misc_feature    6742..6761
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that start with ""ATG"""
     primer_bind     complement(6918..6936)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7004..7108
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7109..7177,7178..7969)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7327..7346)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8140..8728
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8629..8648
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(8909..8980)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 gcattcgaga caaggagatt atagcatggc ggcaaacgct ggcgagacga aggtgaagct
       61 ctgggagggc atctgctacc cagagaacat gcgcgatgac tggcaggacg agatagacga
      121 cattcttcaa gtgcctttcg cttatgcggt tcacgacata gaccatgacc agaagagcaa
      181 gcagcgcaag acgcatgcgc acgtcattgt tgtctggggc ggcaacacga cacgcaaggc
      241 aatcatcaac gtcttgaacc gattgagcgc ggacggcaag aagtgctgtt cgtcagccga
      301 gccaatcaac aacatcaggc atgcgtatga ctatctgatt cacgacacgg cttcctgtcg
      361 caagaagggc aaggagctgt atccggtcga agcacgcatc gagggaaaca acttcgacat
      421 tggtcagctc gaacagctct cgactaagga caagcaggac atgctcttcg agttggtcgg
      481 cttcatcatg tgcgagaggt tcgagaccat caacgatttc acggctgcgg cgctgaggga
      541 gtttcccgag cagtaccgcg aaatcatcgt ggggtacaac tcgattctcg agcgctattg
      601 tcgcgggaac tatctgaacg ccgaacgtaa gcgcaagtgc gtggaacagt caggtgccaa
      661 agagtagtcg ggtcaatgca gcaatgcgtg actacggggc ggcgtggtct gcgctcgcgc
      721 agatctaagc cgactcgttc acggattgcg gaattgacgc gacggccaac cgaatagacc
      781 agacttgatt aagggtcgat ggacgctctg ctccatcgac cctttccttg cccgtgtctc
      841 gactggatga gacctgcaga atcgggcggc tcattatttt tcgctaacta cgtccgaaaa
      901 ataatgtgtc cgaaaaataa tgtgacaaat gacaaatgac aaatgaccac aggggggcca
      961 ctacgacggc ccccctaata gtgccgtgtg ccgtgtgccg aaccgcaggt agattgaggg
     1021 gtggttcgga ggggcgaagt ccctccgttc ggcgctcgcg ccgatggccc gagggccgtg
     1081 agcgaagccg cgaacgggca agggctgggg ggtctcgggg ggaacgcccg agcgggtgca
     1141 gggctggccc tgccacgtcc ttagcgtagc taaggtctaa cgtgctagac tttagtcgat
     1201 taagtctgta cggttcggag gtttccatgg ctcacatcgc gaaatacaag gctccatcgg
     1261 tcgggcacat gcttgcccac taccgccgcg accgcagcag cctggagcgc gacaacatcg
     1321 accccgagcg catcaagaac gacttggtgg tcggccacta cacgaacaag gacggcaagc
     1381 tcgttgtggg gcgcgtggag ccgcgcgatg gcgagccgaa ctggggcacg gtggagagcc
     1441 gcatcgagcg cgtgaacgag gcccagaagg ccgcagggaa gcgtgccacg cgcaaggatg
     1501 cggtcgttat ggctgacgtt gttgtgacgc ttcccgacaa tgtgcgcaag ggcgatgagg
     1561 acaggttctt ccgtctcacg tactggtacc tgtcgaagaa gttcggcatc gagaacatga
     1621 tgggcgggta cgtccacaag gacgaggtgt tgaaggatgg cacgcccgcc cgcgaccaca
     1681 tgcacgtgcc gttcacgccc atcctagatg gacggttcaa ctacaagcag atgtgccccc
     1741 gcagcttcta ccggagcatg cacaaggagc ttggggatta cctagagggg cgcatgggct
     1801 accgccctgc aatcgagctt gacgaggaga cgcgggcgca gcgtgtatac acggacaagt
     1861 ccgtggatat cgacaaggta cgctgtgcgg tcgataaggc cgtggttgaa cctgcgaagg
     1921 acgaagcggc gcgaatcgtc gcggcggcta gggaagaagc cgccgcttta ctgaatgatg
     1981 ccgaaacgcg taaggcagag cttgttaccg agattgccga caaggagggt gaccttgccg
     2041 agcttgacag ccagctcgag gacgtgaagc tcgatatcga agatgagcaa gaccgattgg
     2101 agtgtcttcg gcaacgagcg gacggagttg cgcgagacat tggagagctt caacccatcg
     2161 ctaccgaggt tcggggctgg gaagctgcgg gaaaagctga gcgcggcgca atcctcgata
     2221 acatcgttgt taagtgcgat ggactcgcga gccgcatcag agcaggagtt gcgggaatgc
     2281 gaatcaaagt ggaagagctt agaagtcgaa tatcgcgccc gctcgaatat ctgatgcgca
     2341 gggaacagcc aaggcaacag agccttaatg acgtgctgcg cgatgcgcag cgggcggcgg
     2401 atgcgtggaa ccgcgaccac gctgcaccgc agaggacgta tcgcgggcgg gctagatgat
     2461 gtgttatagt agtacctgta atacacgaca aggaggtgcg acgcgaaaaa accccgctga
     2521 agcggggttt tttgcgcacc acagcagaca gcagaaaaca ttctgaacgc taggaagccg
     2581 gtatgattgt tacatataag gggaagaaaa atttctttta ggtacttgct ttcctaaaac
     2641 tgatgtgata caatgattta atccagaaaa ggagtaaaaa atatgcggca aggtattctt
     2701 aaataaaact ataatcaaat agtgggaaca aaggattatg atagctcctt ttgtagggac
     2761 ttagtttttt gtacccaatt taagaatact tttgccttat caattttgac atatccccaa
     2821 aaacagcaat cacaaacagg tgtatgctgt atatgtgtat gtccgcaact tataatcccc
     2881 agtggtaaaa gtattttact gctggggatt tttatgccct ttggggctgt aaagggagga
     2941 caatcacatg aaaataatca atattggaat tcttgcccat gtagacgctg gaaagacgac
     3001 cttgacggag agcctgctat atgccagcgg agccatttca gaaccgggga gcgtcgaaaa
     3061 agggacaacg aggacggaca ccatgttttt ggagcggcag cgtgggatta ccattcaagc
     3121 ggcagtcact tccttccagt ggcacagatg taaagttaac attgtggata cgcccggcca
     3181 catggatttt ttggcggagg tgtaccgctc tttggctgtt ttagatgggg ccatcttggt
     3241 gatctccgct aaagatggcg tgcaggccca gacccgtatt ctgttccatg ccctgcggaa
     3301 aatgaacatt cccaccgtta tctttatcaa caagatcgac caggctggcg ttgatttgca
     3361 gagcgtggtt cagtctgttc gggataagct ctccgccgat attatcatca agcagacggt
     3421 gtcgctgtcc ccggaaatag tcctggagga aaataccgac atagaagcat gggatgcggt
     3481 catcgaaaat aacgatgaat tattggaaaa gtatatcgca ggagaaccaa tcagccggga
     3541 aaaacttgcg cgggaggaac agcagcgggt tcaagacgcc tccctgttcc cagtctatca
     3601 tggcagcgcc aaaaatggcc ttggcattca accgttgatg gatgcggtga cagggctgtt
     3661 ccaaccgatt ggggaacagg ggggcgccgc cctatgcggc agcgttttca aggttgagta
     3721 caccgattgc ggccagcggc gtgtctatct acggttatac agcggaacgc tgcgcctgcg
     3781 ggatacggtg gccctggccg ggagagaaaa gctgaaaatc acagagatgc gtattccatc
     3841 caaaggggaa attgttcgga cagacaccgc ttatcagggt gaaattgtta tccttcccag
     3901 cgacagcgtg aggttaaacg atgtattagg ggaccaaacc cggctccctc gtaaaaggtg
     3961 gcgcgaggac cccctcccca tgctgcggac ggcgattgcg ccgaaaacgg cagcgcaaag
     4021 agaacggctg ctggacgctc ttacgcaact tgcggatact gacccgcttt tgcgttgcga
     4081 agtggattcc atcacccatg agatcattct ttcttttttg ggccgggtgc agttggaggt
     4141 tgtttccgct ttgctgtcgg aaaaatacaa gcttgaaaca gtggtaaagg aaccctccgt
     4201 catttatatg gagcggccgc tcaaagcagc cagccacacc atccatatcg aggtgccgcc
     4261 caacccgttt tgggcatcca taggactgtc tgttacacca ctctcgcttg gctccggtgt
     4321 acaatacgag agccgggttt cgctgggata cttgaaccag agttttcaaa acgctgtcag
     4381 ggatggtatc cgttacgggc tggagcaggg cttgttcggc tggaacgtaa cggactgtaa
     4441 gatttgcttt gaatacgggc tttattacag tccggtcagc acgccggcgg acttccgctc
     4501 attggccccg attgtattgg aacaggcatt gaaggaatcg gggacgcagc tgctggaacc
     4561 ttatctctcc ttcatcctct atgcgcccca ggaatacctt tccagggctt atcatgatgc
     4621 accgaaatac tgtgccacca tcgaaacggc ccaggtaaaa aaggatgaag ttgtctttac
     4681 tggcgagatt cccgcccgct gtatacaggc ataccgtact gatctggcct tttacaccaa
     4741 cgggcggagc gtatgcctta cagagctgaa aggatatcag gccgctgtcg gtcagccggt
     4801 catccagccc cgccgtccaa acagccgcct ggacaaggtg cgccatatgt ttcagaaggt
     4861 aatgtaaatc gttaagggat caactttggg agagagttca aaattgatcc tttttttata
     4921 acaggaatta aataataaaa aagccggatt aataatctgg ctttttatat tctctctcta
     4981 gtatataaac gcagaaaggc ccacccgaag gtgagccagt gtgagtcgac tagttcagac
     5041 cgcctcgtcc tgcgcggccg tctcgtgggc caggtcgatg agctcctggc gattgtccac
     5101 gcccgtcttc tggtagatgt ggcggatatg cgtgctcacc gtgcccgccg agatgaacag
     5161 cgtctcctgg atgcgcgaga tggtgcggcc ctccagcagc agcggaagca cgtcggtctc
     5221 gcgcggcgac agggcgaagc gctccgcgat cagcgcgcac ggggcgtccg ggtcgtcgcc
     5281 cgtggggggt tcggcggcca cctccacatc catcaggctg acctcgccga tgccgatttt
     5341 gatgaggtcg gtctccgtga acaggaacaa atgcgcgatg aacagcagcg acacggccac
     5401 cagcgtgacc agcgtgaggt cgaacatgct gaccccggtc gcctccagcg aatgggcgaa
     5461 cagctggcct gcggtctcgc cgccgtacag cgccacgaac cccaggccga tcgtccgcgc
     5521 cgggctcacg cggaagctgg acgccacgtc gatgcacacc acgcacagca ccagcacgaa
     5581 gcagtggtag ccgccgaaga taagcgcgct cgaatacgtg ttgttgtcgg ccatgaacgg
     5641 cgtggacaag catcccaagc acagcatcag catggagacg cggtacgaga agaacacgct
     5701 gtcgcgctgc aggaacacgg ctatcaggca gatggccaac gctccgatga agcccgccag
     5761 aaacaggtac gtgccgaaga tgtcctgcac ctcgtaggtt ttgaaccccg acaccacgtc
     5821 gaagaacccc atcaacatgc cgatgagcac cgcgcccaga agcgccttcg cgaacagggt
     5881 cgccgtatgg cgcgacaaag gctgctcggg ctcgcgcggc agctgcggcg tatcgggcgc
     5941 gctgcgcctg agagctgccg ccgaaagcac gggcatgaca acgatgagca cgatgacggg
     6001 tatcgccggc cctacgatca gcaagaaccc cacgacgaac gcgatgcaca gcgacgaggc
     6061 aagctccaca agcgccaagc ggcctcccag ccccgactcg agctcgatcc agcacaagaa
     6121 cagcacggcc gagcccgtgc cggtcatgac cgcgctcagg cccagcacca gcatgccgac
     6181 gggcgtggta ggatcggaga acggcgtgag cacgctgccc gcgctcatca tgatggccga
     6241 tcccagcacg atggagcggt tgccgctcac ccgctcgcgc cgcacgatga cggagacgaa
     6301 cagcatggtc accaccatgg ccaccagcga tatgatcagg ctgacgtaca gcgagccgcg
     6361 cacgtccgac gagaagccgt acggcacgat gctgtagaac atcatgagca tccaaagcca
     6421 ttgcaaggcc gcgcccgcga tgcgcttgag ggaaaggctc gtccccaacg cggtatccca
     6481 tgcactccga agcttttcca tgccccaccc cctgtgagcc cgtacataac gtcgctcgcg
     6541 ccgacccctc tgtcgacggg ttcgattata cggaaaagat gagcggccct ctttgaggaa
     6601 actattctag tttcagaata atttatgacc tacggttaaa gtttccccag ttcaaagcac
     6661 tatcgaattt ttcatgatca caagatcata agaatttatg atgcggtcga acctgctttc
     6721 caacctttgg acgatgctca tctagaagcg gccgcgaatt cagaatagta atacaggatc
     6781 cgaatcgttt cagttgccgc cggattaata atcaaataac cgttcctaaa aaatgccctc
     6841 ttgggttatc aagagggtca ttatatttcg caaaaaaccc cgcttcggcg gggttttttc
     6901 gccttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat
     6961 aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
     7021 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
     7081 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
     7141 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
     7201 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
     7261 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
     7321 agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg
     7381 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
     7441 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
     7501 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
     7561 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
     7621 accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact
     7681 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
     7741 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
     7801 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
     7861 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     7921 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     7981 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     8041 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     8101 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     8161 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     8221 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     8281 tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     8341 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     8401 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     8461 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     8521 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     8581 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     8641 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     8701 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcccgag atgcgccgcg
     8761 tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat
     8821 tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga
     8881 ggtgccgccg gcatgagctc ctctagtaga gagcgttcac cgacaaacaa cagataaaac
     8941 gaaaggccca gtctttcgac tgagcctttc gttttatttg aaaaagaaaa gccccagcaa
     9001 gcttggcggc agcaggg
//
