LOCUS       sequence-37586-e         806 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 806)
  AUTHORS   Petzold AM, Balciunas D, Sivasubbu S, Clark KJ, Bedell VM, Westcot 
            SE, Myers SR, Moulder GL, Thomas MJ, Ekker SC
  TITLE     Nicotine response genetics in the zebrafish.
  JOURNAL   Proc Natl Acad Sci U S A. 2009 Nov 3;106(44):18662-7. Epub 2009 Oct 
            26.
  PUBMED    19858493
REFERENCE   2  (bases 1 to 806)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2009-11-3"; volume: "106(44)"; pages: 
            "18662-7. Epub 2009 Oct 26"
COMMENT     Sequence Label: pGBT-PX
FEATURES             Location/Qualifiers
     source          1..806
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    join(51..62,63..225)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     protein_bind    complement(276..309)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 atactcaagc tatgcatcca acgcgttggg agctctccca tatggtcgag cagaggtgta
       61 aaaagtactc aaaaatttta ctcaagtgaa agtacaagta cttagggaaa attttactca
      121 attaaaagta aaagtatctg gctagaatct tacttgagta aaagtaaaaa agtactccat
      181 taaaattgta cttgagtatt aaggaagtaa aagtaaaagc aagaaagaaa actagagatt
      241 cttgtttaag ctcatggtag ggataacagg gtaatataac ttcgtatagc atacattata
      301 cgaagttatc gttaattaag aagttcctat actttctaga gaataggaag atctccggac
      361 gaaacaggaa gctgactcca catggtcaca tgctcactga agtgttgact tccctgacag
      421 ctgtgcactt tctaaaccgg ttttctcatt catttacagt tcagccgatg atgaaattgc
      481 cgcactggtt gttagcaacg tagccggtgt gtgaaagatg gattcgcggg aattccgaag
      541 ctagaactag taattaagtc tcagccaccg ttaactgaac atgtcaaaac ctgtggagac
      601 tgttgagatt tgatgttctg aaaagataaa gcctataaat aaaatgttgc ccaaatttcc
      661 tgcctgatgt ttttctttgt ctttgctaca tggctttgct gctcggatcg gctcactctg
      721 tgtatgccac gttcactttg tactctcctt ctcacggtag gtttattatt tttagatgtg
      781 cagttagttt ctgtgaaata acacac
//
