LOCUS       sequence-375865-        8782 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For knocking p2a-tdTomato into LMX1A locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8782)
  AUTHORS   Xu P, He H, Gao Q, Zhou Y, Wu Z, Zhang X, Sun L, Hu G, Guan Q, You 
            Z, Zhang X, Zheng W, Xiong M, Chen Y
  TITLE     Human midbrain dopaminergic neuronal differentiation markers predict
            cell therapy outcomes in a Parkinson's disease model.
  JOURNAL   J Clin Invest. 2022 Jul 15;132(14). pii: 156768. doi: 
            10.1172/JCI156768.
  PUBMED    35700056
REFERENCE   2  (bases 1 to 8782)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Clin 
            Invest."; date: "2022-07-15"; volume: "132(14). pii: 156768. doi"; 
            pages: " 10.1172/JCI156768"
COMMENT     Sequence Label: PL752-LMX1A-LA-p2a-tdTomato-hGHpA-PGK-puroR-LM...
FEATURES             Location/Qualifiers
     source          1..8782
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(16..444)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(126..147)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     338..357
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        471..575
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(576..644,645..1436)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(794..813)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1607..2195
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2096..2115
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2349..2366
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2483..2504
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2519..2524,2525..2542,2543..2549)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2557..2573
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2562..2584
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2581..2597
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2581..2597
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        2618..2636
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             3666..3722
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             3723..5153
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
     polyA_signal    5218..5694
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(5417..5436)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     promoter        5755..6254
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(5790..5813)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     6187..6206
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             6294..6893
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APHNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6294..6313)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     complement(8588..8607)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8589..8607)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8614..8631)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8614..8630)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8623..8645)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 ttaatatttt gttaaaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat
       61 aggccgaaat cggcaaaatc ccttataaat caaaagaata gaccgagata gggttgagtg
      121 ttgttccagt ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc
      181 gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc atcaccctaa tcaagttttt
      241 tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc cgatttagag
      301 cttgacgggg aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg
      361 gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc
      421 ttaatgcgcc gctacagggc gcgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc
      481 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
      541 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
      601 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
      661 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
      721 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
      781 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac
      841 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
      901 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
      961 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
     1021 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
     1081 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
     1141 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
     1201 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
     1261 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
     1321 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
     1381 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
     1441 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     1501 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     1561 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     1621 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     1681 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     1741 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     1801 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     1861 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     1921 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     1981 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     2041 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     2101 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     2161 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     2221 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     2281 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
     2341 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc
     2401 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
     2461 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt
     2521 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca
     2581 caggaaacag ctatgaccat gattacgcca agctcgaaat taaccctcac taaagggaac
     2641 aaaagctggt accgtgatag ccaccctctt ggcttcctta gaaggcacag gtcacatctg
     2701 taccattctt tactccacag ctatcggaaa acagggcaaa cacatttttt tgcaaattct
     2761 tttcagagtc tcagggattg tgttacatga agtatccctg tgatgagtcc tttcataaaa
     2821 tgaatattta aagccccatg acaagttgta taaactccag ttttcacctc tcctatttac
     2881 ttgaagcaga ggcagaacat tctttgcctc cagatcctgg ttgcagtgat ttctcagctg
     2941 ttccaatgtg tgccctactg gcttccctat caaaggtgta aattagttga aggtaggcac
     3001 ctaaccttcc ttttctttgc attacaaatt tggggtaaca gctgatagta gacccttggt
     3061 gtttgaaaga acaaatgtta tcacaggagt tttcattagc ttcactcaaa gagctttctt
     3121 tttgttttgt ttttttgttt gttggtttta ccatccctcc ttcaagcatc atttttcagg
     3181 cccaagtcct ataggagctc tgccctgtct ctccagcctc tggtgacatg tcctgcctac
     3241 tccccacact ccctgtctag acattgtgac atttgcttat gctgcactca atttagcact
     3301 ttgtcatatc ctggtttaga ttgttttcac ttgttgcact ttggacttga gccccctaaa
     3361 gagaatatag actttttgta gaaagtttca cctggagcct gtgctcaggc aacacttgtt
     3421 gaattcagct gaatatccta gcactgaaat gagtcacttc ttcaagaccc tcatgccagt
     3481 gtttcatctc catttcaggt gccgagcccc ttttccatga cctggatagc gacgacacct
     3541 ccctcagtaa cctgggtgat tgtttcctag caacctcaga agctgggcct ctgcagtcca
     3601 gagtgggaaa ccccattgac catctgtact ccatgcagaa ttcttacttc acatctggat
     3661 ccggcgcaac aaacttctct ctgctgaaac aagccggaga tgtcgaagag aatcctggac
     3721 cgatggtgag caagggcgag gaggtcatca aagagttcat gcgcttcaag gtgcgcatgg
     3781 agggctccat gaacggccac gagttcgaga tcgagggcga gggcgagggc cgcccctacg
     3841 agggcaccca gaccgccaag ctgaaggtga ccaagggcgg ccccctgccc ttcgcctggg
     3901 acatcctgtc cccccagttc atgtacggct ccaaggcgta cgtgaagcac cccgccgaca
     3961 tccccgatta caagaagctg tccttccccg agggcttcaa gtgggagcgc gtgatgaact
     4021 tcgaggacgg cggtctggtg accgtgaccc aggactcctc cctgcaggac ggcacgctga
     4081 tctacaaggt gaagatgcgc ggcaccaact tcccccccga cggccccgta atgcagaaga
     4141 agaccatggg ctgggaggcc tccaccgagc gcctgtaccc ccgcgacggc gtgctgaagg
     4201 gcgagatcca ccaggccctg aagctgaagg acggcggcca ctacctggtg gagttcaaga
     4261 ccatctacat ggccaagaag cccgtgcaac tgcccggcta ctactacgtg gacaccaagc
     4321 tggacatcac ctcccacaac gaggactaca ccatcgtgga acagtacgag cgctccgagg
     4381 gccgccacca cctgttcctg gggcatggca ccggcagcac cggcagcggc agctccggca
     4441 ccgcctcctc cgaggacaac aacatggccg tcatcaaaga gttcatgcgc ttcaaggtgc
     4501 gcatggaggg ctccatgaac ggccacgagt tcgagatcga gggcgagggc gagggccgcc
     4561 cctacgaggg cacccagacc gccaagctga aggtgaccaa gggcggcccc ctgcccttcg
     4621 cctgggacat cctgtccccc cagttcatgt acggctccaa ggcgtacgtg aagcaccccg
     4681 ccgacatccc cgattacaag aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga
     4741 tgaacttcga ggacggcggt ctggtgaccg tgacccagga ctcctccctg caggacggca
     4801 cgctgatcta caaggtgaag atgcgcggca ccaacttccc ccccgacggc cccgtaatgc
     4861 agaagaagac catgggctgg gaggcctcca ccgagcgcct gtacccccgc gacggcgtgc
     4921 tgaagggcga gatccaccag gccctgaagc tgaaggacgg cggccactac ctggtggagt
     4981 tcaagaccat ctacatggcc aagaagcccg tgcaactgcc cggctactac tacgtggaca
     5041 ccaagctgga catcacctcc cacaacgagg actacaccat cgtggaacag tacgagcgct
     5101 ccgagggccg ccaccacctg ttcctgtacg gcatggacga gctgtacaag taatgagaat
     5161 tctcaatttc tgagaactgt cattctcgga aattgaagcg ctgctcgaga gatctacggg
     5221 tggcatccct gtgacccctc cccagtgcct ctcctggccc tggaagttgc cactccagtg
     5281 cccaccagcc ttgtcctaat aaaattaagt tgcatcattt tgtctgacta ggtgtccttc
     5341 tataatatta tggggtggag gggggtggta tggagcaagg ggcaagttgg gaagacaacc
     5401 tgtagggcct gcggggtcta ttgggaacca agctggagtg cagtggcaca atcttggctc
     5461 actgcaatct ccgcctcctg ggttcaagcg attctcctgc ctcagcctcc cgagttgttg
     5521 ggattccagg catgcatgac caggctcagc taatttttgt ttttttggta gagacggggt
     5581 ttcaccatat tggccaggct ggtctccaac tcctaatctc aggtgatcta cccaccttgg
     5641 cctcccaaat tgctgggatt acaggcgtga accactgctc ccttccctgt cctttaggtc
     5701 tgaagaggag tttacgtcca gccacgcgta ggatctcgac ctcgaaattc taccgggtag
     5761 gggaggcgct tttcccaagg cagtctggag catgcgcttt agcagccccg ctgggcactt
     5821 ggcgctacac aagtggcctc tggcctcgca cacattccac atccaccggt aggcgccaac
     5881 cgactccgtt ctttggtggc cccttcgcgc caccttctac tcctccccta gtcaggaagt
     5941 tcccccccgc cccgcagctc gcgtcgtgca ggacgtgaca aatggaagta gcacgtctca
     6001 ctagtctcgt gcagatggac agcaccgctg agcaatggaa gcgggtaggc ctttggggca
     6061 gcggccaata gcagctttgc tccttcgctt tctgggctca gaggctggga aggggtgggt
     6121 ccgggggcgg gctcaggggc gggctcaggg gcggggcggg cgcccgaagg tcctccggag
     6181 gcccggcatt ctgcacgctt caaaagcgca cgtctgccgc gctgttctcc tcttcctcat
     6241 ctccgggcct ttcgacctgc atccatctag atctcgagca gctgaagctt accatgaccg
     6301 agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc
     6361 tcgccgccgc gttcgccgac taccccgcca cgcgccacac cgtcgatccg gaccgccaca
     6421 tcgagcgggt caccgagctg caagaactct tcctcacgcg cgtcgggctc gacatcggca
     6481 aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg
     6541 aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc ggttcccggc
     6601 tggccgcgca gcaacagatg gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt
     6661 ggttcctggc caccgtcggc gtctcgcccg accaccaggg caagggtctg ggcagcgccg
     6721 tcgtgctccc cggagtggag gcggccgagc gcgccggggt gcccgccttc ctggagacct
     6781 ccgcgcccca caacctcccc ttctacgagc ggctcggctt caccgtcacc gccgacgtcg
     6841 aggtgcccga aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc tgacgcccgc
     6901 cccacgaccc gcagcgcccg accgaaagga gcgcacgacc ccatgcatcg atgatatcag
     6961 atccccggga tgcagaaatt gatgatctat taaacaataa agatgtccac taaaatggaa
     7021 gtttttcctg tcatactttg ttaagaaggg tgagaacaga gtacctacat tttgaatgga
     7081 aggattggag ctacgggggt gggggtgggg tgggattaga taaatgcctg ctctttactg
     7141 aaggctcttt actattgctt tatgataatg tttcatagtt ggatatcata atttaaacaa
     7201 gcaaaaccaa attaagggcc agctcattcc tcccactcat gatctataga tctatagatc
     7261 tctcgtggga tcattgtttt tctcttgatt cccactttgt ggttctaagt actgtggttt
     7321 ccaaatgtgt cagtttcata gcctgaagaa cgagatcagc agcctctgtt ccacatacac
     7381 ttcattctca gtattgtttt gccaagttct aattccatca gaagctggtc gagatccgga
     7441 acccttaatt caatttctga gaactgtcat tctcggaaat tgaggatcct gagtcttccc
     7501 ctagagttct gtgactaggc tcccatatgg aacaaccata ttctttgagg ggtcactggc
     7561 tttaggacag ggaggccagg gaagaggtgg gttggggagg gagttttgtt ggggatgctg
     7621 ttgtataatg atatggtgta gctcagcatt tccaaagact gaatacatta tggattgcat
     7681 agtttaatgt ttctaataag agtcttagca ttagatatga agacgtgttt atcattaagg
     7741 acagagactt ttaatataga cattctcatg caaactagat acttagggac tcctaacaac
     7801 ttcccaccat gtcggggaag ctcttgtcaa gaggtgcata tgtctatcca tctacacacc
     7861 aatagacaga aggacagata gatagatgtg tgtgtgtgag tgtgtaacct ttcgtatttt
     7921 accctcaaag tttattccta attataacag acaccaactg tacagcaaaa gtaactttat
     7981 tttcagtgtg aactatattt aaggaaatgc ttgatgcact taagttataa aatgagataa
     8041 tttactttta taaactttat ttttagcttg acaagacttg tcagcagggc agagagggct
     8101 gctccaccta gccccatagc tttgagtgct ggggttcatt ctgttttcag agtgtctttc
     8161 agatctggaa agaaattctg tgtggctgat ggtgttctct cttgcattct tgctctcttt
     8221 ggggttgaat cactgggcag gggtgggaca gaataatctc tgatcatgtt ctgagaaaat
     8281 gtaaagccca gactcctggg ctttctttta aattctgaca agtggttgtt gggcagtgct
     8341 aggatgattg gttcagctct tgagcttcag catctgcaaa tgtggatgag gctaatagta
     8401 tgtacctacc tcactgggaa acaccaaggc ttaattcatt cccaggacac atgagcaggg
     8461 ctgagactaa tatctgatat ttgtttaaga tacaaccagg ccactcactt ggcaaaggag
     8521 ggtacatagg gttgcagagc aggagggctc ctgaacgcgg ccgccaccgc ggtggagctc
     8581 caattcgccc tatagtgagt cgtattacaa ttcactggcc gtcgttttac aacgtcgtga
     8641 ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag
     8701 ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa
     8761 tggcgaatgg aaattgtaag cg
//
