LOCUS       sequence-375868-         898 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cell line generation via recombination-mediated cassette exchange 
            (RMCE) and stable expression of MBD1.MBD-R22A-13XL-BASU.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 898)
  AUTHORS   Villasenor R, Pfaendler R, Ambrosi C, Butz S, Giuliani S, Bryan E, 
            Sheahan TW, Gable AL, Schmolka N, Manzo M, Wirz J, Feller C, von 
            Mering C, Aebersold R, Voigt P, Baubec T
  TITLE     ChromID identifies the protein interactome at chromatin marks.
  JOURNAL   Nat Biotechnol. 2020 Mar 2. pii: 10.1038/s41587-020-0434-2. doi: 
            10.1038/s41587-020-0434-2.
  PUBMED    32123383
REFERENCE   2  (bases 1 to 898)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2020 Mar 2. pii: 10.1038/s41587-020-0434-2. doi: 
            10.1038/s41587-020-0434-2."
COMMENT     Sequence Label: pv9_MBD1_MBD-R22A-13XL-BASU
FEATURES             Location/Qualifiers
     source          1..898
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      30..39
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             39..62
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             348..368
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             654..680
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 gaattcatta ttgtttttcc tcctttgccg ccaccatgga ctacaaagac gatgacgaca
       61 aggatgaaga gtctggttct gtttctggtg gtgatgctga gtcctggcag gactgcccag
      121 ccctgggccc tggctggaaa cgccgagagt cctttgcaaa gtcaggggcc tcgtttggac
      181 gctcagacat ctattaccag agccccacag gagaaaagat ccgaagcaaa gttgagctga
      241 ctcggtactt gggccctgca tgtgatctta ccctgtttga cttcagacaa ggcaccttat
      301 gccatcccat cggtggcgga ggctctggag gtggaggctc cgcacccccc aaaaagaaac
      361 gcaaggttgg ataccgggat ccacacgtgg gtaggagcgg ttcgttcgga tcccatcttt
      421 tttcggaatt cggatccggt ggaggcgggt ctggaggcgg gggtagtggc gggggtggaa
      481 gcgggggtgg aggcgggtcg ggtggcggag gtagcggagg cggtggaagt ggaggcggtg
      541 gaagtggtgg aggaggttct ggtggcggag gtagcggtgg cggaggtagc ggaggcggtg
      601 gaagtggtgg cggaggtagc ggaggcggtg gatcggcgcg ccgtttcaag aactacccat
      661 acgatgtgcc tgactatgct ggcaagttgt ctgaatcaga gattaggttt ggactgaaaa
      721 cggaggtgat gggccaacat cttatctatc acgatgtttt gtccagcaca caaaagacag
      781 cccatgaatt gcaaataaca atgcccccga gggtaccctc gtggttgcag ataaacagac
      841 agccggtagg ggcgggatga gccgagtgtg gcattctcaa gaaggaaacg gagtatgg
//
