LOCUS       sequence-37587-e         678 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 678)
  AUTHORS   Clark KJ, Balciunas D, Pogoda HM, Ding Y, Westcot SE, Bedell VM, 
            Greenwood TM, Urban MD, Skuster KJ, Petzold AM, Ni J, Nielsen AL, 
            Patowary A, Scaria V, Sivasubbu S, Xu X, Hammerschmidt M, Ekker SC
  TITLE     In vivo protein trapping produces a functional expression codex of 
            the vertebrate proteome.
  JOURNAL   Nat Methods. 2011 Jun;8(6):506-15. Epub 2011 May 8.
  PUBMED    21552255
REFERENCE   2  (bases 1 to 678)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods."; date: "2011-06"; volume: "8(6)"; pages: "506-15. Epub 
            2011 May 8"
COMMENT     Sequence Label: pGBT-R16
FEATURES             Location/Qualifiers
     source          1..678
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    join(19..30,31..193)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     protein_bind    238..271
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 ctctcccata tggtcgagca gaggtgtaaa aagtactcaa aaattttact caagtgaaag
       61 tacaagtact tagggaaaat tttactcaat taaaagtaaa agtatctggc tagaatctta
      121 cttgagtaaa agtaaaaaag tactccatta aaattgtact tgagtattaa ggaagtaaaa
      181 gtaaaagcaa gaaagaaaac tagagattct tgtttaagct gtagggataa cagggtaata
      241 acttcgtata atgtatgcta tacgaagtta tcagcatgca aaccgttctc tgaagcctct
      301 cgtctgtccc gtttcctcat tttaagttct cctgttcatt ggttcacttc ctccaggctt
      361 tgtgtcctct gagctcctga gtttctcctc ttctcagcag gttatctaaa ctttgcctgc
      421 ctgttttgca gttccttctg cactttttgt tcctttactc tggatttccg actaacccct
      481 gcatggaagt gtggattgtg tgctgcagac acttgaacat ccagttaacg tctccatcct
      541 ctctttgcag atcatgttgg agaccttcaa cacccccgcc cccgggtacc ggaaggtgcc
      601 tcctccgagg acgtcatcaa ggagttcatg cgcttcaagg tgcgcatgga gggctccgtg
      661 aacggccacg agttcgag
//
