LOCUS       sequence-375870-         888 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cell line generation via recombination-mediated cassette exchange 
            (RMCE) and stable expression of MBD1.MBD-WT-13XL-BASU.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 888)
  AUTHORS   Villasenor R, Pfaendler R, Ambrosi C, Butz S, Giuliani S, Bryan E, 
            Sheahan TW, Gable AL, Schmolka N, Manzo M, Wirz J, Feller C, von 
            Mering C, Aebersold R, Voigt P, Baubec T
  TITLE     ChromID identifies the protein interactome at chromatin marks.
  JOURNAL   Nat Biotechnol. 2020 Mar 2. pii: 10.1038/s41587-020-0434-2. doi: 
            10.1038/s41587-020-0434-2.
  PUBMED    32123383
REFERENCE   2  (bases 1 to 888)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2020 Mar 2. pii: 10.1038/s41587-020-0434-2. doi: 
            10.1038/s41587-020-0434-2."
COMMENT     Sequence Label: pv9_MBD1_MBD-WT-13XL-BASU
FEATURES             Location/Qualifiers
     source          1..888
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      25..34
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             34..57
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             343..363
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             649..675
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 cattattgtt tttcctcctt tgccgccacc atggactaca aagacgatga cgacaaggat
       61 gaagagtctg gttctgtttc tggtggtgat gctgagtcct ggcaggactg cccagccctg
      121 ggccctggct ggaaacgccg agagtccttt cgaaagtcag gggcctcgtt tggacgctca
      181 gacatctatt accagagccc cacaggagaa aagatccgaa gcaaagttga gctgactcgg
      241 tacttgggcc ctgcatgtga tcttaccctg tttgacttca gacaaggcac cttatgccat
      301 cccatcggtg gcggaggctc tggaggtgga ggctccgcac cccccaaaaa gaaacgcaag
      361 gttggatacc gggatccaca cgtgggtagg agcggttcgt tcggatccca tcttttttcg
      421 gaattcggat ccggtggagg cgggtctgga ggcgggggta gtggcggggg tggaagcggg
      481 ggtggaggcg ggtcgggtgg cggaggtagc ggaggcggtg gaagtggagg cggtggaagt
      541 ggtggaggag gttctggtgg cggaggtagc ggtggcggag gtagcggagg cggtggaagt
      601 ggtggcggag gtagcggagg cggtggatcg gcgcgccgtt tcaagaacta cccatacgat
      661 gtgcctgact atgctggcaa gttgtctgaa tcagagatta ggtttggact gaaaacggag
      721 gtgatgggcc aacatcttat ctatcacgat gttttgtcca gcacacaaaa gacagcccat
      781 gaattggcaa ataacaatgc ccccgagggt accctcgtgg ttgcagataa acagacagcc
      841 ggtaggggcg ggatgagccg agtgtggcat tctcaagaag gaaacgga
//
