LOCUS       sequence-375882-        9247 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  E. coli - Eggerthella lenta shuttle plasmid (TetR), hcdR 
            complementation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9247)
  AUTHORS   Dong X, Guthrie BGH, Alexander M, Noecker C, Ramirez L, Glasser NR, 
            Turnbaugh PJ, Balskus EP
  TITLE     Genetic manipulation of the human gut bacterium Eggerthella lenta 
            reveals a widespread family of transcriptional regulators.
  JOURNAL   Nat Commun. 2022 Dec 9;13(1):7624. doi: 10.1038/s41467-022-33576-3.
  PUBMED    36494336
REFERENCE   2  (bases 1 to 9247)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-12-9"; volume: "13(1):7624. doi"; pages: " 
            10.1038/s41467-022-33576-3"
COMMENT     Sequence Label: pXD70Tet(HcdR)-HcdR
FEATURES             Location/Qualifiers
     source          1..9247
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(4957..4984)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     misc_feature    6939..6958
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that start with ""ATG"""
     primer_bind     complement(7115..7133)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7201..7305
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7306..7374,7375..8166)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7524..7543)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8337..8925
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8826..8845
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(9106..9177)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 aaacgctggc gagacgaagg tgaagctctg ggagggcatc tgctacccag agaacatgcg
       61 cgatgactgg caggacgaga tagacgacat tcttcaagtg cctttcgctt atgcggttca
      121 cgacatagac catgaccaga agagcaagca gcgcaagacg catgcgcacg tcattgttgt
      181 ctggggcggc aacacgacac gcaaggcaat catcaacgtc ttgaaccgat tgagcgcgga
      241 cggcaagaag tgctgttcgt cagccgagcc aatcaacaac atcaggcatg cgtatgacta
      301 tctgattcac gacacggctt cctgtcgcaa gaagggcaag gagctgtatc cggtcgaagc
      361 acgcatcgag ggaaacaact tcgacattgg tcagctcgaa cagctctcga ctaaggacaa
      421 gcaggacatg ctcttcgagt tggtcggctt catcatgtgc gagaggttcg agaccatcaa
      481 cgatttcacg gctgcggcgc tgagggagtt tcccgagcag taccgcgaaa tcatcgtggg
      541 gtacaactcg attctcgagc gctattgtcg cgggaactat ctgaacgccg aacgtaagcg
      601 caagtgcgtg gaacagtcag gtgccaaaga gtagtcgggt caatgcagca atgcgtgact
      661 acggggcggc gtggtctgcg ctcgcgcaga tctaagccga ctcgttcacg gattgcggaa
      721 ttgacgcgac ggccaaccga atagaccaga cttgattaag ggtcgatgga cgctctgctc
      781 catcgaccct ttccttgccc gtgtctcgac tggatgagac ctgcagaatc gggcggctca
      841 ttatttttcg ctaactacgt ccgaaaaata atgtgtccga aaaataatgt gacaaatgac
      901 aaatgacaaa tgaccacagg ggggccacta cgacggcccc cctaatagtg ccgtgtgccg
      961 tgtgccgaac cgcaggtaga ttgaggggtg gttcggaggg gcgaagtccc tccgttcggc
     1021 gctcgcgccg atggcccgag ggccgtgagc gaagccgcga acgggcaagg gctggggggt
     1081 ctcgggggga acgcccgagc gggtgcaggg ctggccctgc cacgtcctta gcgtagctaa
     1141 ggtctaacgt gctagacttt agtcgattaa gtctgtacgg ttcggaggtt tccatggctc
     1201 acatcgcgaa atacaaggct ccatcggtcg ggcacatgct tgcccactac cgccgcgacc
     1261 gcagcagcct ggagcgcgac aacatcgacc ccgagcgcat caagaacgac ttggtggtcg
     1321 gccactacac gaacaaggac ggcaagctcg ttgtggggcg cgtggagccg cgcgatggcg
     1381 agccgaactg gggcacggtg gagagccgca tcgagcgcgt gaacgaggcc cagaaggccg
     1441 cagggaagcg tgccacgcgc aaggatgcgg tcgttatggc tgacgttgtt gtgacgcttc
     1501 ccgacaatgt gcgcaagggc gatgaggaca ggttcttccg tctcacgtac tggtacctgt
     1561 cgaagaagtt cggcatcgag aacatgatgg gcgggtacgt ccacaaggac gaggtgttga
     1621 aggatggcac gcccgcccgc gaccacatgc acgtgccgtt cacgcccatc ctagatggac
     1681 ggttcaacta caagcagatg tgcccccgca gcttctaccg gagcatgcac aaggagcttg
     1741 gggattacct agaggggcgc atgggctacc gccctgcaat cgagcttgac gaggagacgc
     1801 gggcgcagcg tgtatacacg gacaagtccg tggatatcga caaggtacgc tgtgcggtcg
     1861 ataaggccgt ggttgaacct gcgaaggacg aagcggcgcg aatcgtcgcg gcggctaggg
     1921 aagaagccgc cgctttactg aatgatgccg aaacgcgtaa ggcagagctt gttaccgaga
     1981 ttgccgacaa ggagggtgac cttgccgagc ttgacagcca gctcgaggac gtgaagctcg
     2041 atatcgaaga tgagcaagac cgattggagt gtcttcggca acgagcggac ggagttgcgc
     2101 gagacattgg agagcttcaa cccatcgcta ccgaggttcg gggctgggaa gctgcgggaa
     2161 aagctgagcg cggcgcaatc ctcgataaca tcgttgttaa gtgcgatgga ctcgcgagcc
     2221 gcatcagagc aggagttgcg ggaatgcgaa tcaaagtgga agagcttaga agtcgaatat
     2281 cgcgcccgct cgaatatctg atgcgcaggg aacagccaag gcaacagagc cttaatgacg
     2341 tgctgcgcga tgcgcagcgg gcggcggatg cgtggaaccg cgaccacgct gcaccgcaga
     2401 ggacgtatcg cgggcgggct agatgatgtg ttatagtagt acctgtaata cacgacaagg
     2461 aggtgcgacg cgaaaaaacc ccgctgaagc ggggtttttt gcgcaccaca gcagacagca
     2521 gaaaacattc tgaacgctag gaagccggta tgattgttac atataagggg aagaaaaatt
     2581 tcttttaggt acttgctttc ctaaaactga tgtgatacaa tgatttaatc cagaaaagga
     2641 gtaaaaaata tgcggcaagg tattcttaaa taaaactata atcaaatagt gggaacaaag
     2701 gattatgata gctccttttg tagggactta gttttttgta cccaatttaa gaatactttt
     2761 gccttatcaa ttttgacata tccccaaaaa cagcaatcac aaacaggtgt atgctgtata
     2821 tgtgtatgtc cgcaacttat aatccccagt ggtaaaagta ttttactgct ggggattttt
     2881 atgccctttg gggctgtaaa gggaggacaa tcacatgaaa ataatcaata ttggaattct
     2941 tgcccatgta gacgctggaa agacgacctt gacggagagc ctgctatatg ccagcggagc
     3001 catttcagaa ccggggagcg tcgaaaaagg gacaacgagg acggacacca tgtttttgga
     3061 gcggcagcgt gggattacca ttcaagcggc agtcacttcc ttccagtggc acagatgtaa
     3121 agttaacatt gtggatacgc ccggccacat ggattttttg gcggaggtgt accgctcttt
     3181 ggctgtttta gatggggcca tcttggtgat ctccgctaaa gatggcgtgc aggcccagac
     3241 ccgtattctg ttccatgccc tgcggaaaat gaacattccc accgttatct ttatcaacaa
     3301 gatcgaccag gctggcgttg atttgcagag cgtggttcag tctgttcggg ataagctctc
     3361 cgccgatatt atcatcaagc agacggtgtc gctgtccccg gaaatagtcc tggaggaaaa
     3421 taccgacata gaagcatggg atgcggtcat cgaaaataac gatgaattat tggaaaagta
     3481 tatcgcagga gaaccaatca gccgggaaaa acttgcgcgg gaggaacagc agcgggttca
     3541 agacgcctcc ctgttcccag tctatcatgg cagcgccaaa aatggccttg gcattcaacc
     3601 gttgatggat gcggtgacag ggctgttcca accgattggg gaacaggggg gcgccgccct
     3661 atgcggcagc gttttcaagg ttgagtacac cgattgcggc cagcggcgtg tctatctacg
     3721 gttatacagc ggaacgctgc gcctgcggga tacggtggcc ctggccggga gagaaaagct
     3781 gaaaatcaca gagatgcgta ttccatccaa aggggaaatt gttcggacag acaccgctta
     3841 tcagggtgaa attgttatcc ttcccagcga cagcgtgagg ttaaacgatg tattagggga
     3901 ccaaacccgg ctccctcgta aaaggtggcg cgaggacccc ctccccatgc tgcggacggc
     3961 gattgcgccg aaaacggcag cgcaaagaga acggctgctg gacgctctta cgcaacttgc
     4021 ggatactgac ccgcttttgc gttgcgaagt ggattccatc acccatgaga tcattctttc
     4081 ttttttgggc cgggtgcagt tggaggttgt ttccgctttg ctgtcggaaa aatacaagct
     4141 tgaaacagtg gtaaaggaac cctccgtcat ttatatggag cggccgctca aagcagccag
     4201 ccacaccatc catatcgagg tgccgcccaa cccgttttgg gcatccatag gactgtctgt
     4261 tacaccactc tcgcttggct ccggtgtaca atacgagagc cgggtttcgc tgggatactt
     4321 gaaccagagt tttcaaaacg ctgtcaggga tggtatccgt tacgggctgg agcagggctt
     4381 gttcggctgg aacgtaacgg actgtaagat ttgctttgaa tacgggcttt attacagtcc
     4441 ggtcagcacg ccggcggact tccgctcatt ggccccgatt gtattggaac aggcattgaa
     4501 ggaatcgggg acgcagctgc tggaacctta tctctccttc atcctctatg cgccccagga
     4561 atacctttcc agggcttatc atgatgcacc gaaatactgt gccaccatcg aaacggccca
     4621 ggtaaaaaag gatgaagttg tctttactgg cgagattccc gcccgctgta tacaggcata
     4681 ccgtactgat ctggcctttt acaccaacgg gcggagcgta tgccttacag agctgaaagg
     4741 atatcaggcc gctgtcggtc agccggtcat ccagccccgc cgtccaaaca gccgcctgga
     4801 caaggtgcgc catatgtttc agaaggtaat gtaaatcgtt aagggatcaa ctttgggaga
     4861 gagttcaaaa ttgatccttt ttttataaca ggaattaaat aataaaaaag ccggattaat
     4921 aatctggctt tttatattct ctctctagta tataaacgca gaaaggccca cccgaaggtg
     4981 agccagtgtg agtcgactag tctattcggc gggatccgca agctcgacga ggcgcatgag
     5041 ctcctgctgg ctgtggacgt tcgtcttctt gtagacgtgg cggatgtggg ttttcgcggt
     5101 gccttccgag atgtagagct tttcctggat gtacgcggcg ttgtgcccct tcgccagaaa
     5161 gaacatgatc tccgtctcgc gccgggaaag caggtaggtg ttggcaaccg tctcgcactt
     5221 cgcgcggaag cggccgcctc cctttcgctc ttcgccctcg tctgctcggc gtgcgctgct
     5281 gagttccagc agcgaagggg cgcgccgcgc ggtcggttcg ggttcgggcg cgatctcggc
     5341 gtcaggtgca ggcgcgttgg tcgctgccgt cgcgttgttt ccagattgcg cggccgcggc
     5401 ggctccctcg ccggccgatc ccgcttgccc tagcggccgc acgcggtcgc tcaattccaa
     5461 cgacacggct ttcaccagcg gacacggtgc gacgatggcc tcgatctcgc gctcgcgcgg
     5521 cagcagcgcg tatgccacca ccataatcag cagcacgatg acgatgacgc cctgttcgcc
     5581 gaacggaagc agatgcaccc agttcgccgc cacgatggga aacagcgaac ccgccagccc
     5641 cgccagcgcc aacatgccgc gtcccaggcc gaacacgtag atgggcgaca agcggaagcg
     5701 ttgcgccagc tcgccgaaga atatccacat cagcgcctcg aagcacaggt agcccatcac
     5761 caagaacatg gtggcgaacg tgttgtcgct catttcggaa agcggcagga agaacagcgt
     5821 gacggcgatg aaggggacga gcgggcggaa gtaccggctc catttgtcgc cgccgccaag
     5881 ggccacgatg gtcacgagga tgactacgga ggcgaatccg gcggccagca gcatggcgat
     5941 ctggtcgcct acgttcgagg cgggcacgat gtactggatg gacgtttgcc gcagcgtgcc
     6001 caacgccacg ccgaacacga acacgggaat gccgaagcgc aggaagaact tggcatggtt
     6061 caccggaagc ggtttgaaga tgggaacctc gttgcgctcg gaatacggcg ccggcgtttt
     6121 gttccagagg atggcgagct ccagcagcgg gatgatgccc gcgaggatgc cggcgatggg
     6181 gaacggcccg taatgcaggc cgatggcgta caccccgatg gcgatggaga tggcgatgga
     6241 cgagttcagc acgatggaag cgctgtcgca gcgcgcgaac gcgacgcccc agaacagaat
     6301 caggatggcg gagccgatgc ccgtcatgac gcccgatacc gcttccagcg ccgggccgct
     6361 gatcacgggc gtcgcaagca gcagcaacgt tccggccgac atgagcgccg cgcccgccgc
     6421 aagcgtcttc ttcgagatgt acgtcctcaa cagccgctgg tcggtgatgc cggcgaacaa
     6481 caggcacagc ccgaacacga cgatggagac gaggaacggc agcgacgcgt ggctgccgta
     6541 catgccggtg acgtaggttt gcgtgccgaa gatggtgggc gttccgaaca tgacggcgta
     6601 cacccaagcc tggtgcagtc cgtagcccac cgagagcaag ctcaggtcgc cgaacgcggt
     6661 gtcgtcgccg aagtcggcgt tgacggtgat gcgaggaacg cgaatcccca tgaagccccc
     6721 tccttgattc cctgggcatc atcataccgt gcagaggttg ttttttctag cgaggtgagc
     6781 gagtcgatca gggttttgcg ctcgatgcgc aaacgtttga tctggggtaa tagaaaaata
     6841 atcctctgag gatgatgttc ggaagggcgc ccgtctgctt tcgagggcgt gctgaggcgt
     6901 cgcggtgctg catcgggccg gcttgtgaag aataacatct agaagcggcc gcgaattcag
     6961 aatagtaata caggatccga atcgtttcag ttgccgccgg attaataatc aaataaccgt
     7021 tcctaaaaaa tgccctcttg ggttatcaag agggtcatta tatttcgcaa aaaaccccgc
     7081 ttcggcgggg ttttttcgcc ttgaagacga aagggcctcg tgatacgcct atttttatag
     7141 gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg
     7201 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
     7261 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
     7321 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
     7381 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
     7441 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
     7501 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg
     7561 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
     7621 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
     7681 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
     7741 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
     7801 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca
     7861 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
     7921 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
     7981 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca
     8041 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
     8101 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
     8161 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
     8221 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     8281 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     8341 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     8401 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     8461 agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag
     8521 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     8581 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     8641 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     8701 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     8761 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     8821 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     8881 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     8941 gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca
     9001 agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt
     9061 ggtgaatccg ttagcgaggt gccgccggca tgagctcctc tagtagagag cgttcaccga
     9121 caaacaacag ataaaacgaa aggcccagtc tttcgactga gcctttcgtt ttatttgaaa
     9181 aagaaaagcc ccagcaagct tggcggcagc aggggcattc gagacaagga gattatagca
     9241 tggcggc
//
