LOCUS       sequence-375891-       10048 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For use in RBP tethering screen.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10048)
  AUTHORS   Luo EC, Nathanson JL, Tan FE, Schwartz JL, Schmok JC, Shankar A, 
            Markmiller S, Yee BA, Sathe S, Pratt GA, Scaletta DB, Ha Y, Hill DE,
            Aigner S, Yeo GW
  TITLE     Large-scale tethered function assays identify factors that regulate 
            mRNA stability and translation.
  JOURNAL   Nat Struct Mol Biol. 2020 Oct;27(10):989-1000. doi: 
            10.1038/s41594-020-0477-6. Epub 2020 Aug 17.
  PUBMED    32807991
REFERENCE   2  (bases 1 to 10048)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41594-020-0477-6"; journalName: "Nat Struct Mol Biol"; 
            date: "2020-10"; volume: "27"; issue: "10"; pages: "989-1000"
COMMENT     Sequence Label: ADAR
FEATURES             Location/Qualifiers
     source          1..10048
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     primer_bind     1196..1215
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1196..1214
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    1293..1317
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5005..5029)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5154..5195
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(5650..5667)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5656..5880
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6013..6032)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    6164..6211
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             6219..7241
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             6219..7241
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    7371..7492
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7408..7427)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7462..7481
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7541..7557)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7541..7557)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7554..7576)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7565..7581
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7589..7595,7596..7613,7614..7619))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7634..7655
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7772..7789)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7943..8531)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8023..8042)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8702..9493,9494..9562))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9325..9344
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9563..9667)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9742..9761)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gaattagctt ggtactaata
     1201 cgactcacta tagggagacc caagctggct aggtaagctt ggtaccgagc tcggatccac
     1261 tagtccagtg tggtggaatt ctgcagatat caacaagttt gtacaaaaaa gcaggctcca
     1321 ccatgaatcc gcggcagggg tattccctca gcggatacta cacccatcca tttcaaggct
     1381 atgagcacag acagctcaga taccagcagc ctgggccagg atcttccccc agtagtttcc
     1441 tgcttaagca aatagaattt ctcaaggggc agctcccaga agcaccggtg attggaaagc
     1501 agacaccgtc actgccacct tccctcccag gactccggcc aaggtttcca gtactacttg
     1561 cctccagtac cagaggcagg caagtggaca tcaggggtgt ccccaggggc gtgcatctcg
     1621 gaagtcaggg gctccagaga gggttccagc atccttcacc acgtggcagg agtctgccac
     1681 agagaggtgt tgattgcctt tcctcacatt tccaggaact gagtatctac caagatcagg
     1741 aacaaaggat cttaaagttc ctggaagagc ttggggaagg gaaggccacc acagcacatg
     1801 atctgtctgg gaaacttggg actccgaaga aagaaatcaa tcgagtttta tactccctgg
     1861 caaagaaggg caagctacag aaagaggcag gaacaccccc tttgtggaaa atcgcggtct
     1921 ccactcaggc ttggaaccag cacagcggag tggtaagacc agacggtcat agccaaggag
     1981 ccccaaactc agacccgagt ttggaaccgg aagacagaaa ctccacatct gtctcagaag
     2041 atcttcttga gccttttatt gcagtctcag ctcaggcttg gaaccagcac agcggagtgg
     2101 taagaccaga cagtcatagc caaggatccc caaactcaga cccaggtttg gaacctgaag
     2161 acagcaactc cacatctgcc ttggaagatc ctcttgagtt tttagacatg gccgagatca
     2221 aggagaaaat ctgcgactat ctcttcaatg tgtctgactc ctctgccctg aatttggcta
     2281 aaaatattgg ccttaccaag gcccgagata taaatgctgt gctaattgac atggaaaggc
     2341 agggggatgt ctatagacaa gggacaaccc ctcccatatg gcatttgaca gacaagaagc
     2401 gagagaggat gcaaatcaag agaaatacga acagtgttcc tgaaaccgct ccagctgcaa
     2461 tccctgagac caaaagaaac gcagagttcc tcacctgtaa tatacccaca tcaaatgcct
     2521 caaataacat ggtaaccaca gaaaaagtgg agaatgggca ggaacctgtc ataaagttag
     2581 aaaacaggca agaggccaga ccagaaccag caagactgaa accacctgtt cattacaatg
     2641 gcccctcaaa agcagggtat gttgactttg aaaatggcca gtgggccaca gatgacatcc
     2701 cagatgactt gaatagtatc cgcgcagcac caggtgagtt tcgagccatc atggagatgc
     2761 cctccttcta cagtcatggc ttgccacggt gttcacccta caagaaactg acagagtgcc
     2821 agctgaagaa ccccatcagc gggctgttag aatatgccca gttcgctagt caaacctgtg
     2881 agttcaacat gatagagcag agtggaccac cccatgaacc tcgatttaaa ttccaggttg
     2941 tcatcaatgg ccgagagttt cccccagctg aagctggaag caagaaagtg gccaagcagg
     3001 atgcagctat gaaagccatg acaattctgc tagaggaagc caaagccaag gacagtggaa
     3061 aatcagaaga atcatcccac tattccacag agaaagaatc agagaagact gcagagtccc
     3121 agacccccac cccttcagcc acatccttct tttctgggaa gagccccgtc accacactgc
     3181 ttgagtgtat gcacaaattg gggaactcct gcgaattccg tctcctgtcc aaagaaggcc
     3241 ctgcccatga acccaagttc caatactgtg ttgcagtggg agcccaaact ttccccagtg
     3301 tgagtgctcc cagcaagaaa gtggcaaagc agatggccgc agaggaagcc atgaaggccc
     3361 tgcatgggga ggcgaccaac tccatggctt ctgataacca gcctgaaggt atgatctcag
     3421 agtcacttga taacttggaa tccatgatgc ccaacaaggt caggaagatt ggcgagctcg
     3481 tgagatacct gaacaccaac cctgtgggtg gccttttgga gtacgcccgc tcccatggct
     3541 ttgctgctga attcaagttg gtcgaccagt ccggacctcc tcacgagccc aagttcgttt
     3601 accaagcaaa agttgggggt cgctggttcc cagccgtctg cgcacacagc aagaagcaag
     3661 gcaagcagga agcagcagat gcggctctcc gtgtcttgat tggggagaac gagaaggcag
     3721 aacgcatggg tttcacagag gtaaccccag tgacaggggc cagtctcaga agaactatgc
     3781 tcctcctctc aaggtcccca gaagcacagc caaagacact ccctctcact ggcagcacct
     3841 tccatgacca gatagccatg ctgagccacc ggtgcttcaa cactctgact aacagcttcc
     3901 agccctcctt gctcggccgc aagattctgg ccgccatcat tatgaaaaaa gactctgagg
     3961 acatgggtgt cgtcgtcagc ttgggaacag ggaatcgctg tgtaaaagga gattctctca
     4021 gcctaaaagg agaaactgtc aatgactgcc atgcagaaat aatctcccgg agaggcttca
     4081 tcaggtttct ctacagtgag ttaatgaaat acaactccca gactgcgaag gatagtatat
     4141 ttgaacctgc taagggagga gaaaagctcc aaataaaaaa gactgtgtca ttccatctgt
     4201 atatcagcac tgctccgtgt ggagatggcg ccctctttga caagtcctgc agcgaccgtg
     4261 ctatggaaag cacagaatcc cgccactacc ctgtcttcga gaatcccaaa caaggaaagc
     4321 tccgcaccaa ggtggagaac ggagaaggca caatccctgt ggaatccagt gacattgtgc
     4381 ctacgtggga tggcattcgg ctcggggaga gactccgtac catgtcctgt agtgacaaaa
     4441 tcctacgctg gaacgtgctg ggcctgcaag gggcactgtt gacccacttc ctgcagccca
     4501 tttatctcaa atctgtcaca ttgggttacc ttttcagcca agggcatctg acccgtgcta
     4561 tttgctgtcg tgtgacaaga gatgggagtg catttgagga tggactacga catcccttta
     4621 ttgtcaacca ccccaaggtt ggcagagtca gcatatatga ttccaaaagg caatccggga
     4681 agactaagga gacaagcgtc aactggtgtc tggctgatgg ctatgacctg gagatcctgg
     4741 acggtaccag aggcactgtg gatgggccac ggaatgaatt gtcccgggtc tccaaaaaga
     4801 acatttttct tctatttaag aagctctgct ccttccgtta ccgcagggat ctactgagac
     4861 tctcctatgg tgaggccaag aaagctgccc gtgactacga gacggccaag aactacttca
     4921 aaaaaggcct gaaggatatg ggctatggga actggattag caaaccccag gaggaaaaga
     4981 acttttatct ctgcccagta ttggacccag ctttcttgta caaagtggtt gatatccagc
     5041 acagtggcgg ccgctcgagt ctagagggcc cgcggttcga aaccagcgga ggcggcggaa
     5101 gcgggggagg aggcagcggc ggaggcggca gcgggggcgg cggaagcgct accggtaagc
     5161 ctatccctaa ccctctcctc ggtctcgatt ctacgcgtac cggtatggct tctaacttta
     5221 ctcagttcgt tctcgtcgac aatggcggaa ctggcgacgt gactgtcgcc ccaagcaact
     5281 tcgctaacgg ggtcgctgaa tggatcagct ctaactcgcg atcacaggct tacaaagtaa
     5341 cctgtagcgt tcgtcagagc tctgcgcaga atcgcaaata caccatcaaa gtcgaggtgc
     5401 ctaaagtggc aacccagact gttggtggtg aagagcttcc tgtagccgga tggagatctt
     5461 acttaaatat ggaactaacc attccaattt tcgccacgaa ttccgactgc gagcttattg
     5521 ttaaggcaat gcaaggtctc ctaaaagatg gaaacccgat tccctcggcc atcgcggcca
     5581 actccggcat ctacggaggt ggaggtggat ctcgtacggg ttagtaatga gtttaaaccc
     5641 gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg
     5701 tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa
     5761 ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca
     5821 gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg ggctctatgg
     5881 cttctgaggc ggaaagaacc agctggggct ctagggggta tccccacgcg ccctgtagcg
     5941 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
     6001 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
     6061 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc
     6121 tcgaccccaa aaaacttgat tagggtgatg gttcacgtac ctagaagttc ctattccgaa
     6181 gttcctattc tctagaaagt ataggaactt ccttggccaa aaagcctgaa ctcaccgcga
     6241 cgtctgtcga gaagtttctg atcgaaaagt tcgacagcgt ctccgacctg atgcagctct
     6301 cggagggcga agaatctcgt gctttcagct tcgatgtagg agggcgtgga tatgtcctgc
     6361 gggtaaatag ctgcgccgat ggtttctaca aagatcgtta tgtttatcgg cactttgcat
     6421 cggccgcgct cccgattccg gaagtgcttg acattgggga attcagcgag agcctgacct
     6481 attgcatctc ccgccgtgca cagggtgtca cgttgcaaga cctgcctgaa accgaactgc
     6541 ccgctgttct gcagccggtc gcggaggcca tggatgcgat cgctgcggcc gatcttagcc
     6601 agacgagcgg gttcggccca ttcggaccgc aaggaatcgg tcaatacact acatggcgtg
     6661 atttcatatg cgcgattgct gatccccatg tgtatcactg gcaaactgtg atggacgaca
     6721 ccgtcagtgc gtccgtcgcg caggctctcg atgagctgat gctttgggcc gaggactgcc
     6781 ccgaagtccg gcacctcgtg cacgcggatt tcggctccaa caatgtcctg acggacaatg
     6841 gccgcataac agcggtcatt gactggagcg aggcgatgtt cggggattcc caatacgagg
     6901 tcgccaacat cttcttctgg aggccgtggt tggcttgtat ggagcagcag acgcgctact
     6961 tcgagcggag gcatccggag cttgcaggat cgccgcggct ccgggcgtat atgctccgca
     7021 ttggtcttga ccaactctat cagagcttgg ttgacggcaa tttcgatgat gcagcttggg
     7081 cgcagggtcg atgcgacgca atcgtccgat ccggagccgg gactgtcggg cgtacacaaa
     7141 tcgcccgcag aagcgcggcc gtctggaccg atggctgtgt agaagtactc gccgatagtg
     7201 gaaaccgacg ccccagcact cgtccgaggg caaaggaata gcacgtacta cgagatttcg
     7261 attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct
     7321 ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccacccc aacttgttta
     7381 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
     7441 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
     7501 gtataccgtc gacctctagc tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt
     7561 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
     7621 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt
     7681 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
     7741 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
     7801 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
     7861 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     7921 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     7981 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     8041 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     8101 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     8161 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     8221 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     8281 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     8341 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
     8401 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     8461 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     8521 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     8581 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     8641 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     8701 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     8761 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     8821 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     8881 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     8941 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     9001 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     9061 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     9121 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     9181 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     9241 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     9301 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     9361 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     9421 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     9481 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     9541 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
     9601 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     9661 ttccgcgcac atttccccga aaagtgccac ctgacgtcga cggatcggga gatctcccga
     9721 tcccctatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatct
     9781 gctccctgct tgtgtgttgg aggtcgctga gtagtgcgcg agcaaaattt aagctacaac
     9841 aaggcaaggc ttgaccgaca attgcatgaa gaatctgctt agggttaggc gttttgcgct
     9901 gcttcgcgat gtacgggcca gatatacgcg ttgacattga ttattgacta ggcttttgca
     9961 aaaagctttg caaagatgga taaagtttta aacagagagg aatctttgca gctaatggac
    10021 cttctaggtc ttgaaaggag tgggaatt
//
