LOCUS       sequence-375919-        8801 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  E. coli - Eggerthella lenta shuttle plasmid (TetR), dadR(Delta-DBD) 
            complementation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8801)
  AUTHORS   Dong X, Guthrie BGH, Alexander M, Noecker C, Ramirez L, Glasser NR, 
            Turnbaugh PJ, Balskus EP
  TITLE     Genetic manipulation of the human gut bacterium Eggerthella lenta 
            reveals a widespread family of transcriptional regulators.
  JOURNAL   Nat Commun. 2022 Dec 9;13(1):7624. doi: 10.1038/s41467-022-33576-3.
  PUBMED    36494336
REFERENCE   2  (bases 1 to 8801)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-12-9"; volume: "13(1):7624. doi"; pages: " 
            10.1038/s41467-022-33576-3"
COMMENT     Sequence Label: pXD70Tet(DadRmt1)-DadR(Delta-DBD)
FEATURES             Location/Qualifiers
     source          1..8801
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(4985..5012)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     misc_feature    6521..6540
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that start with ""ATG"""
     primer_bind     complement(6697..6715)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6783..6887
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6888..6956,6957..7748)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7106..7125)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7919..8507
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8408..8427
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(8688..8759)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 cgagacaagg agattatagc atggcggcaa acgctggcga gacgaaggtg aagctctggg
       61 agggcatctg ctacccagag aacatgcgcg atgactggca ggacgagata gacgacattc
      121 ttcaagtgcc tttcgcttat gcggttcacg acatagacca tgaccagaag agcaagcagc
      181 gcaagacgca tgcgcacgtc attgttgtct ggggcggcaa cacgacacgc aaggcaatca
      241 tcaacgtctt gaaccgattg agcgcggacg gcaagaagtg ctgttcgtca gccgagccaa
      301 tcaacaacat caggcatgcg tatgactatc tgattcacga cacggcttcc tgtcgcaaga
      361 agggcaagga gctgtatccg gtcgaagcac gcatcgaggg aaacaacttc gacattggtc
      421 agctcgaaca gctctcgact aaggacaagc aggacatgct cttcgagttg gtcggcttca
      481 tcatgtgcga gaggttcgag accatcaacg atttcacggc tgcggcgctg agggagtttc
      541 ccgagcagta ccgcgaaatc atcgtggggt acaactcgat tctcgagcgc tattgtcgcg
      601 ggaactatct gaacgccgaa cgtaagcgca agtgcgtgga acagtcaggt gccaaagagt
      661 agtcgggtca atgcagcaat gcgtgactac ggggcggcgt ggtctgcgct cgcgcagatc
      721 taagccgact cgttcacgga ttgcggaatt gacgcgacgg ccaaccgaat agaccagact
      781 tgattaaggg tcgatggacg ctctgctcca tcgacccttt ccttgcccgt gtctcgactg
      841 gatgagacct gcagaatcgg gcggctcatt atttttcgct aactacgtcc gaaaaataat
      901 gtgtccgaaa aataatgtga caaatgacaa atgacaaatg accacagggg ggccactacg
      961 acggcccccc taatagtgcc gtgtgccgtg tgccgaaccg caggtagatt gaggggtggt
     1021 tcggaggggc gaagtccctc cgttcggcgc tcgcgccgat ggcccgaggg ccgtgagcga
     1081 agccgcgaac gggcaagggc tggggggtct cggggggaac gcccgagcgg gtgcagggct
     1141 ggccctgcca cgtccttagc gtagctaagg tctaacgtgc tagactttag tcgattaagt
     1201 ctgtacggtt cggaggtttc catggctcac atcgcgaaat acaaggctcc atcggtcggg
     1261 cacatgcttg cccactaccg ccgcgaccgc agcagcctgg agcgcgacaa catcgacccc
     1321 gagcgcatca agaacgactt ggtggtcggc cactacacga acaaggacgg caagctcgtt
     1381 gtggggcgcg tggagccgcg cgatggcgag ccgaactggg gcacggtgga gagccgcatc
     1441 gagcgcgtga acgaggccca gaaggccgca gggaagcgtg ccacgcgcaa ggatgcggtc
     1501 gttatggctg acgttgttgt gacgcttccc gacaatgtgc gcaagggcga tgaggacagg
     1561 ttcttccgtc tcacgtactg gtacctgtcg aagaagttcg gcatcgagaa catgatgggc
     1621 gggtacgtcc acaaggacga ggtgttgaag gatggcacgc ccgcccgcga ccacatgcac
     1681 gtgccgttca cgcccatcct agatggacgg ttcaactaca agcagatgtg cccccgcagc
     1741 ttctaccgga gcatgcacaa ggagcttggg gattacctag aggggcgcat gggctaccgc
     1801 cctgcaatcg agcttgacga ggagacgcgg gcgcagcgtg tatacacgga caagtccgtg
     1861 gatatcgaca aggtacgctg tgcggtcgat aaggccgtgg ttgaacctgc gaaggacgaa
     1921 gcggcgcgaa tcgtcgcggc ggctagggaa gaagccgccg ctttactgaa tgatgccgaa
     1981 acgcgtaagg cagagcttgt taccgagatt gccgacaagg agggtgacct tgccgagctt
     2041 gacagccagc tcgaggacgt gaagctcgat atcgaagatg agcaagaccg attggagtgt
     2101 cttcggcaac gagcggacgg agttgcgcga gacattggag agcttcaacc catcgctacc
     2161 gaggttcggg gctgggaagc tgcgggaaaa gctgagcgcg gcgcaatcct cgataacatc
     2221 gttgttaagt gcgatggact cgcgagccgc atcagagcag gagttgcggg aatgcgaatc
     2281 aaagtggaag agcttagaag tcgaatatcg cgcccgctcg aatatctgat gcgcagggaa
     2341 cagccaaggc aacagagcct taatgacgtg ctgcgcgatg cgcagcgggc ggcggatgcg
     2401 tggaaccgcg accacgctgc accgcagagg acgtatcgcg ggcgggctag atgatgtgtt
     2461 atagtagtac ctgtaataca cgacaaggag gtgcgacgcg aaaaaacccc gctgaagcgg
     2521 ggttttttgc gcaccacagc agacagcaga aaacattctg aacgctagga agccggtatg
     2581 attgttacat ataaggggaa gaaaaatttc ttttaggtac ttgctttcct aaaactgatg
     2641 tgatacaatg atttaatcca gaaaaggagt aaaaaatatg cggcaaggta ttcttaaata
     2701 aaactataat caaatagtgg gaacaaagga ttatgatagc tccttttgta gggacttagt
     2761 tttttgtacc caatttaaga atacttttgc cttatcaatt ttgacatatc cccaaaaaca
     2821 gcaatcacaa acaggtgtat gctgtatatg tgtatgtccg caacttataa tccccagtgg
     2881 taaaagtatt ttactgctgg ggatttttat gccctttggg gctgtaaagg gaggacaatc
     2941 acatgaaaat aatcaatatt ggaattcttg cccatgtaga cgctggaaag acgaccttga
     3001 cggagagcct gctatatgcc agcggagcca tttcagaacc ggggagcgtc gaaaaaggga
     3061 caacgaggac ggacaccatg tttttggagc ggcagcgtgg gattaccatt caagcggcag
     3121 tcacttcctt ccagtggcac agatgtaaag ttaacattgt ggatacgccc ggccacatgg
     3181 attttttggc ggaggtgtac cgctctttgg ctgttttaga tggggccatc ttggtgatct
     3241 ccgctaaaga tggcgtgcag gcccagaccc gtattctgtt ccatgccctg cggaaaatga
     3301 acattcccac cgttatcttt atcaacaaga tcgaccaggc tggcgttgat ttgcagagcg
     3361 tggttcagtc tgttcgggat aagctctccg ccgatattat catcaagcag acggtgtcgc
     3421 tgtccccgga aatagtcctg gaggaaaata ccgacataga agcatgggat gcggtcatcg
     3481 aaaataacga tgaattattg gaaaagtata tcgcaggaga accaatcagc cgggaaaaac
     3541 ttgcgcggga ggaacagcag cgggttcaag acgcctccct gttcccagtc tatcatggca
     3601 gcgccaaaaa tggccttggc attcaaccgt tgatggatgc ggtgacaggg ctgttccaac
     3661 cgattgggga acaggggggc gccgccctat gcggcagcgt tttcaaggtt gagtacaccg
     3721 attgcggcca gcggcgtgtc tatctacggt tatacagcgg aacgctgcgc ctgcgggata
     3781 cggtggccct ggccgggaga gaaaagctga aaatcacaga gatgcgtatt ccatccaaag
     3841 gggaaattgt tcggacagac accgcttatc agggtgaaat tgttatcctt cccagcgaca
     3901 gcgtgaggtt aaacgatgta ttaggggacc aaacccggct ccctcgtaaa aggtggcgcg
     3961 aggaccccct ccccatgctg cggacggcga ttgcgccgaa aacggcagcg caaagagaac
     4021 ggctgctgga cgctcttacg caacttgcgg atactgaccc gcttttgcgt tgcgaagtgg
     4081 attccatcac ccatgagatc attctttctt ttttgggccg ggtgcagttg gaggttgttt
     4141 ccgctttgct gtcggaaaaa tacaagcttg aaacagtggt aaaggaaccc tccgtcattt
     4201 atatggagcg gccgctcaaa gcagccagcc acaccatcca tatcgaggtg ccgcccaacc
     4261 cgttttgggc atccatagga ctgtctgtta caccactctc gcttggctcc ggtgtacaat
     4321 acgagagccg ggtttcgctg ggatacttga accagagttt tcaaaacgct gtcagggatg
     4381 gtatccgtta cgggctggag cagggcttgt tcggctggaa cgtaacggac tgtaagattt
     4441 gctttgaata cgggctttat tacagtccgg tcagcacgcc ggcggacttc cgctcattgg
     4501 ccccgattgt attggaacag gcattgaagg aatcggggac gcagctgctg gaaccttatc
     4561 tctccttcat cctctatgcg ccccaggaat acctttccag ggcttatcat gatgcaccga
     4621 aatactgtgc caccatcgaa acggcccagg taaaaaagga tgaagttgtc tttactggcg
     4681 agattcccgc ccgctgtata caggcatacc gtactgatct ggccttttac accaacgggc
     4741 ggagcgtatg ccttacagag ctgaaaggat atcaggccgc tgtcggtcag ccggtcatcc
     4801 agccccgccg tccaaacagc cgcctggaca aggtgcgcca tatgtttcag aaggtaatgt
     4861 aaatcgttaa gggatcaact ttgggagaga gttcaaaatt gatccttttt ttataacagg
     4921 aattaaataa taaaaaagcc ggattaataa tctggctttt tatattctct ctctagtata
     4981 taaacgcaga aaggcccacc cgaaggtgag ccagtgtgag tcgactagtt cacgcgcacg
     5041 gatccaccgg gtcggccggg tcgtcggcgg cctcttcggg caccgtgccc atcaggctga
     5101 cctcgccgat gccgattttg atgaggtcgg tctccgtgaa caggaacaaa tgcgcgatga
     5161 acagcagcga cacggccacc agcgtgacca gcgtgaggtc gaacatgctg accccggtcg
     5221 cctccagcga atgggcgaac agctggcctg cggtctcgcc gccgtacagc gccacgaacc
     5281 ccaggccgat cgtccgcgcc gggctcacgc ggaagctgga cgccacgtcg atgcacacca
     5341 cgcacagcac cagcacgaag cagtggtagc cgccgaagat aagcgcgctc gaatacgtgt
     5401 tgttgtcggc catgaacggc gtggacaagc atcccaagca cagcatcagc atggagacgc
     5461 ggtacgagaa gaacacgctg tcgcgctgca ggaacacggc tatcaggcag atggccaacg
     5521 ctccgatgaa gcccgccaga aacaggtacg tgccgaagat gtcctgcacc tcgtaggttt
     5581 tgaaccccga caccacgtcg aagaacccca tcaacatgcc gatgagcacc gcgcccagaa
     5641 gcgccttcgc gaacagggtc gccgtatggc gcgacaaagg ctgctcgggc tcgcgcggca
     5701 gctgcggcgt atcgggcgcg ctgcgcctga gagctgccgc cgaaagcacg ggcatgacaa
     5761 cgatgagcac gatgacgggt atcgccggcc ctacgatcag caagaacccc acgacgaacg
     5821 cgatgcacag cgacgaggca agctccacaa gcgccaagcg gcctcccagc cccgactcga
     5881 gctcgatcca gcacaagaac agcacggccg agcccgtgcc ggtcatgacc gcgctcaggc
     5941 ccagcaccag catgccgacg ggcgtggtag gatcggagaa cggcgtgagc acgctgcccg
     6001 cgctcatcat gatggccgat cccagcacga tggagcggtt gccgctcacc cgctcgcgcc
     6061 gcacgatgac ggagacgaac agcatggtca ccaccatggc caccagcgat atgatcaggc
     6121 tgacgtacag cgagccgcgc acgtccgacg agaagccgta cggcacgatg ctgtagaaca
     6181 tcatgagcat ccaaagccat tgcaaggccg cgcccgcgat gcgcttgagg gaaaggctcg
     6241 tccccaacgc ggtatcccat gcactccgaa gcttttccat gccccacccc ctgtgagccc
     6301 gtacataacg tcgctcgcgc cgacccctct gtcgacgggt tcgattatac ggaaaagatg
     6361 agcggccctc tttgaggaaa ctattctagt ttcagaataa tttatgacct acggttaaag
     6421 tttccccagt tcaaagcact atcgaatttt tcatgatcac aagatcataa gaatttatga
     6481 tgcggtcgaa cctgctttcc aacctttgga cgatgctcat ctagaagcgg ccgcgaattc
     6541 agaatagtaa tacaggatcc gaatcgtttc agttgccgcc ggattaataa tcaaataacc
     6601 gttcctaaaa aatgccctct tgggttatca agagggtcat tatatttcgc aaaaaacccc
     6661 gcttcggcgg ggttttttcg ccttgaagac gaaagggcct cgtgatacgc ctatttttat
     6721 aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg
     6781 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     6841 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
     6901 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
     6961 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
     7021 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
     7081 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg
     7141 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
     7201 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
     7261 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
     7321 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
     7381 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg
     7441 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     7501 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     7561 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     7621 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     7681 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     7741 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     7801 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     7861 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     7921 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     7981 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     8041 gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca
     8101 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     8161 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     8221 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     8281 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     8341 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     8401 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     8461 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     8521 cggcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc
     8581 caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga
     8641 gtggtgaatc cgttagcgag gtgccgccgg catgagctcc tctagtagag agcgttcacc
     8701 gacaaacaac agataaaacg aaaggcccag tctttcgact gagcctttcg ttttatttga
     8761 aaaagaaaag ccccagcaag cttggcggca gcaggggcat t
//
