LOCUS       sequence-375920-       13725 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  E. coli - Eggerthella lenta shuttle plasmid (TetR), native 
            promoter-dopamine dehydroxylase from dopamine-metabolizing E. lenta 
            A2 strain with transcriptional regulator DadR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13725)
  AUTHORS   Dong X, Guthrie BGH, Alexander M, Noecker C, Ramirez L, Glasser NR, 
            Turnbaugh PJ, Balskus EP
  TITLE     Genetic manipulation of the human gut bacterium Eggerthella lenta 
            reveals a widespread family of transcriptional regulators.
  JOURNAL   Nat Commun. 2022 Dec 9;13(1):7624. doi: 10.1038/s41467-022-33576-3.
  PUBMED    36494336
REFERENCE   2  (bases 1 to 13725)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-12-9"; volume: "13(1):7624. doi"; pages: " 
            10.1038/s41467-022-33576-3"
COMMENT     Sequence Label: pXD70Tet(DadHR)-DadR-Pdadh-DadhABC(A2)
FEATURES             Location/Qualifiers
     source          1..13725
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(4957..4984)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     misc_feature    11417..11436
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that start with ""ATG"""
     primer_bind     complement(11593..11611)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        11679..11783
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(11784..11852,11853..12644)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(12002..12021)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      12815..13403
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     13304..13323
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(13584..13655)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 aaacgctggc gagacgaagg tgaagctctg ggagggcatc tgctacccag agaacatgcg
       61 cgatgactgg caggacgaga tagacgacat tcttcaagtg cctttcgctt atgcggttca
      121 cgacatagac catgaccaga agagcaagca gcgcaagacg catgcgcacg tcattgttgt
      181 ctggggcggc aacacgacac gcaaggcaat catcaacgtc ttgaaccgat tgagcgcgga
      241 cggcaagaag tgctgttcgt cagccgagcc aatcaacaac atcaggcatg cgtatgacta
      301 tctgattcac gacacggctt cctgtcgcaa gaagggcaag gagctgtatc cggtcgaagc
      361 acgcatcgag ggaaacaact tcgacattgg tcagctcgaa cagctctcga ctaaggacaa
      421 gcaggacatg ctcttcgagt tggtcggctt catcatgtgc gagaggttcg agaccatcaa
      481 cgatttcacg gctgcggcgc tgagggagtt tcccgagcag taccgcgaaa tcatcgtggg
      541 gtacaactcg attctcgagc gctattgtcg cgggaactat ctgaacgccg aacgtaagcg
      601 caagtgcgtg gaacagtcag gtgccaaaga gtagtcgggt caatgcagca atgcgtgact
      661 acggggcggc gtggtctgcg ctcgcgcaga tctaagccga ctcgttcacg gattgcggaa
      721 ttgacgcgac ggccaaccga atagaccaga cttgattaag ggtcgatgga cgctctgctc
      781 catcgaccct ttccttgccc gtgtctcgac tggatgagac ctgcagaatc gggcggctca
      841 ttatttttcg ctaactacgt ccgaaaaata atgtgtccga aaaataatgt gacaaatgac
      901 aaatgacaaa tgaccacagg ggggccacta cgacggcccc cctaatagtg ccgtgtgccg
      961 tgtgccgaac cgcaggtaga ttgaggggtg gttcggaggg gcgaagtccc tccgttcggc
     1021 gctcgcgccg atggcccgag ggccgtgagc gaagccgcga acgggcaagg gctggggggt
     1081 ctcgggggga acgcccgagc gggtgcaggg ctggccctgc cacgtcctta gcgtagctaa
     1141 ggtctaacgt gctagacttt agtcgattaa gtctgtacgg ttcggaggtt tccatggctc
     1201 acatcgcgaa atacaaggct ccatcggtcg ggcacatgct tgcccactac cgccgcgacc
     1261 gcagcagcct ggagcgcgac aacatcgacc ccgagcgcat caagaacgac ttggtggtcg
     1321 gccactacac gaacaaggac ggcaagctcg ttgtggggcg cgtggagccg cgcgatggcg
     1381 agccgaactg gggcacggtg gagagccgca tcgagcgcgt gaacgaggcc cagaaggccg
     1441 cagggaagcg tgccacgcgc aaggatgcgg tcgttatggc tgacgttgtt gtgacgcttc
     1501 ccgacaatgt gcgcaagggc gatgaggaca ggttcttccg tctcacgtac tggtacctgt
     1561 cgaagaagtt cggcatcgag aacatgatgg gcgggtacgt ccacaaggac gaggtgttga
     1621 aggatggcac gcccgcccgc gaccacatgc acgtgccgtt cacgcccatc ctagatggac
     1681 ggttcaacta caagcagatg tgcccccgca gcttctaccg gagcatgcac aaggagcttg
     1741 gggattacct agaggggcgc atgggctacc gccctgcaat cgagcttgac gaggagacgc
     1801 gggcgcagcg tgtatacacg gacaagtccg tggatatcga caaggtacgc tgtgcggtcg
     1861 ataaggccgt ggttgaacct gcgaaggacg aagcggcgcg aatcgtcgcg gcggctaggg
     1921 aagaagccgc cgctttactg aatgatgccg aaacgcgtaa ggcagagctt gttaccgaga
     1981 ttgccgacaa ggagggtgac cttgccgagc ttgacagcca gctcgaggac gtgaagctcg
     2041 atatcgaaga tgagcaagac cgattggagt gtcttcggca acgagcggac ggagttgcgc
     2101 gagacattgg agagcttcaa cccatcgcta ccgaggttcg gggctgggaa gctgcgggaa
     2161 aagctgagcg cggcgcaatc ctcgataaca tcgttgttaa gtgcgatgga ctcgcgagcc
     2221 gcatcagagc aggagttgcg ggaatgcgaa tcaaagtgga agagcttaga agtcgaatat
     2281 cgcgcccgct cgaatatctg atgcgcaggg aacagccaag gcaacagagc cttaatgacg
     2341 tgctgcgcga tgcgcagcgg gcggcggatg cgtggaaccg cgaccacgct gcaccgcaga
     2401 ggacgtatcg cgggcgggct agatgatgtg ttatagtagt acctgtaata cacgacaagg
     2461 aggtgcgacg cgaaaaaacc ccgctgaagc ggggtttttt gcgcaccaca gcagacagca
     2521 gaaaacattc tgaacgctag gaagccggta tgattgttac atataagggg aagaaaaatt
     2581 tcttttaggt acttgctttc ctaaaactga tgtgatacaa tgatttaatc cagaaaagga
     2641 gtaaaaaata tgcggcaagg tattcttaaa taaaactata atcaaatagt gggaacaaag
     2701 gattatgata gctccttttg tagggactta gttttttgta cccaatttaa gaatactttt
     2761 gccttatcaa ttttgacata tccccaaaaa cagcaatcac aaacaggtgt atgctgtata
     2821 tgtgtatgtc cgcaacttat aatccccagt ggtaaaagta ttttactgct ggggattttt
     2881 atgccctttg gggctgtaaa gggaggacaa tcacatgaaa ataatcaata ttggaattct
     2941 tgcccatgta gacgctggaa agacgacctt gacggagagc ctgctatatg ccagcggagc
     3001 catttcagaa ccggggagcg tcgaaaaagg gacaacgagg acggacacca tgtttttgga
     3061 gcggcagcgt gggattacca ttcaagcggc agtcacttcc ttccagtggc acagatgtaa
     3121 agttaacatt gtggatacgc ccggccacat ggattttttg gcggaggtgt accgctcttt
     3181 ggctgtttta gatggggcca tcttggtgat ctccgctaaa gatggcgtgc aggcccagac
     3241 ccgtattctg ttccatgccc tgcggaaaat gaacattccc accgttatct ttatcaacaa
     3301 gatcgaccag gctggcgttg atttgcagag cgtggttcag tctgttcggg ataagctctc
     3361 cgccgatatt atcatcaagc agacggtgtc gctgtccccg gaaatagtcc tggaggaaaa
     3421 taccgacata gaagcatggg atgcggtcat cgaaaataac gatgaattat tggaaaagta
     3481 tatcgcagga gaaccaatca gccgggaaaa acttgcgcgg gaggaacagc agcgggttca
     3541 agacgcctcc ctgttcccag tctatcatgg cagcgccaaa aatggccttg gcattcaacc
     3601 gttgatggat gcggtgacag ggctgttcca accgattggg gaacaggggg gcgccgccct
     3661 atgcggcagc gttttcaagg ttgagtacac cgattgcggc cagcggcgtg tctatctacg
     3721 gttatacagc ggaacgctgc gcctgcggga tacggtggcc ctggccggga gagaaaagct
     3781 gaaaatcaca gagatgcgta ttccatccaa aggggaaatt gttcggacag acaccgctta
     3841 tcagggtgaa attgttatcc ttcccagcga cagcgtgagg ttaaacgatg tattagggga
     3901 ccaaacccgg ctccctcgta aaaggtggcg cgaggacccc ctccccatgc tgcggacggc
     3961 gattgcgccg aaaacggcag cgcaaagaga acggctgctg gacgctctta cgcaacttgc
     4021 ggatactgac ccgcttttgc gttgcgaagt ggattccatc acccatgaga tcattctttc
     4081 ttttttgggc cgggtgcagt tggaggttgt ttccgctttg ctgtcggaaa aatacaagct
     4141 tgaaacagtg gtaaaggaac cctccgtcat ttatatggag cggccgctca aagcagccag
     4201 ccacaccatc catatcgagg tgccgcccaa cccgttttgg gcatccatag gactgtctgt
     4261 tacaccactc tcgcttggct ccggtgtaca atacgagagc cgggtttcgc tgggatactt
     4321 gaaccagagt tttcaaaacg ctgtcaggga tggtatccgt tacgggctgg agcagggctt
     4381 gttcggctgg aacgtaacgg actgtaagat ttgctttgaa tacgggcttt attacagtcc
     4441 ggtcagcacg ccggcggact tccgctcatt ggccccgatt gtattggaac aggcattgaa
     4501 ggaatcgggg acgcagctgc tggaacctta tctctccttc atcctctatg cgccccagga
     4561 atacctttcc agggcttatc atgatgcacc gaaatactgt gccaccatcg aaacggccca
     4621 ggtaaaaaag gatgaagttg tctttactgg cgagattccc gcccgctgta tacaggcata
     4681 ccgtactgat ctggcctttt acaccaacgg gcggagcgta tgccttacag agctgaaagg
     4741 atatcaggcc gctgtcggtc agccggtcat ccagccccgc cgtccaaaca gccgcctgga
     4801 caaggtgcgc catatgtttc agaaggtaat gtaaatcgtt aagggatcaa ctttgggaga
     4861 gagttcaaaa ttgatccttt ttttataaca ggaattaaat aataaaaaag ccggattaat
     4921 aatctggctt tttatattct ctctctagta tataaacgca gaaaggccca cccgaaggtg
     4981 agccagtgtg agtcgactag tttagtacat caggaccact gggtagccca gctggtagat
     5041 cagcacgcga aacatcacgg aggtggccag cgcgccgatg gcggcgacga ccatgagcgt
     5101 cttggaggcg cccaccttct tcttcgccac gaaggcgcag gccgcagcca cgatcacgga
     5161 gacgatggag ccccagaaca tgggcgctcc cgcgccggac accatgacgg tcatcaggct
     5221 gtcgacgtgc gtgggggccg tggtcatcga tgtcgggtcg gcgtagtagc cgaattgcgc
     5281 gatcttcgcc atggcgcagg cgatgacgaa caccacgtcg gccacgatct gcacgagggc
     5341 cgcgatgaac gtcagctgga tgcccgtcgc ctcgacggcc tcgtccttct tcaggatgga
     5401 gatcagccag acggccgcgg cgcccaggag gcacgcgttg cccagataga acgccacgag
     5461 cgccaggccc cacgcgggac gggccggcat gaggtaggag tgcgcggtgg cgaccatcat
     5521 gagcacggca accgcgaggg tcgcccaggc cagcgccttg ggaacgggct tgccgcctcg
     5581 aaggaccacg aaccatgcga cgatgacgat ggccagcgcc acgcatccga tgagctcctg
     5641 ggtgatgccc gacgtgatgt gcccgaagcc gttgaagatg cgctcccagt gctcgaggtg
     5701 caggaaggct ccgatgccgc ctaccaccag cgagacggcg gacgtgatga gcgcggtcat
     5761 ctgcaggttc ctgccctggc ccttgagcgc caggatggac atgccgccca gcgtgccgca
     5821 ggtcaagcat acgaacagcg tgaagatgac gagaggccac tgcgtttcca ttactcatca
     5881 cctccttccc agtgatggtc gcgtaggatg taggagtacg acggcttgtt gcccacgtcg
     5941 ggcagcttgt gcacgtcggc gtcggtgaac ggctccagga actccaccat cttgcggcgc
     6001 ttgcccagcg ggtcctcgga gccctcgaac gactcgtagc cggcctcggt gtcgccgatc
     6061 cagcgggcgt tgccgccgca ctggctgacg cactggggca gctcgccctg cgacagcttc
     6121 tgctcgcaca gcgtgcactt ctcgacgacc ttctcctcct cgttgaggta gcggacgccg
     6181 taggggcagg ccatgacgca gaactggcag ccgatgcact tctccttgtc gatctggatg
     6241 gagccgtcct cggccacgtg cgacgcctcg gtggggcaca ccttcacgca ctcggggttc
     6301 tcgcagtgct ggcagctgat gggcaggaag tacatgtaca cgtcggggta ctgccctgat
     6361 ccgcccggga tggggttcgg ccccacgcgc accactttgt tccagtagct gccgatgggc
     6421 acgttgttga ccgccttgca ggcgaccgag cacgccaggc agcccacgca ccggttgagg
     6481 tcggtgatga tagccttcgt catggtttac tccctccctt cgtaggtagg cagccattcc
     6541 ttcagacggg ggtcgtcgga ggtatggatg atgggcgtgc cggtggagtc gcacggcacg
     6601 gggttgttga acggcgagtt ctcgggcgtg gccttgtaga tcttcacctg gtaggcgcgc
     6661 aggttggacg tgccggagtg cgggtcctgg gcgtagtggt cgatcagctg gttcacctgg
     6721 gagaactgcc agccgtggcc ggcgtccttc acctcggggt accaccacgt gtgctcgagg
     6781 ttgatgacgt cttccttcac gccgtagtac aggtcagcca cttcgcggat cttgccccac
     6841 tcggtttcga tccacaccca gtcgccctgc tcgatgccgt actcggcggc cgtcttcggg
     6901 ttgatctcca cgcgcggcac gggccacagc tcgcggcacc agggaagctg gcgatgctcg
     6961 gagtggaagt acaccgggat gcgacggccg gtggtggcgg tcagcgggta ctccttgatg
     7021 cggtcgccgt ccttcgggcc gtgaggcggc tcggtgtagg tgggaagcct ccactcggga
     7081 tggtcgggat ggtgcgattc catgacggtg gaccagatct cctgcttcat cgtcggggtg
     7141 aaccagcccg gcttccagtc gccgatgccc ttgccggccg tgtagtcgag gcggccccac
     7201 acgcggtcgc gtgcgcgcat cgcgccggtc tggtagcggc ggtacgtgcc ccagttgcgc
     7261 ggctcgatgt ccttcgcctg ccaccagccg tgctcctgga aggccttgac gtactcgtcc
     7321 cacgtcttgt acttgggcgt gacctcggcc atcggaacgc cgtggcgctc gaacgtgggc
     7381 ttgccgttct cgacgtgcca cgtggtgtag gtcagctcgt cgtcggtgag cagcttgatg
     7441 gagtcgtcca gctgccagtt gatgtcgggc cactcgttgc ccggctcgtc ggtccacttc
     7501 cagttcatgc ggcgggcgag gtccatgacg atctcgggat cgtacttggc ttccgcgggc
     7561 ggctgcacgc acttgaccgt ggcgcccatg gcgccggcgg aaccttggct ggcgcgcggc
     7621 gagttgagct cgatccaatg ggccaccggc aggatgatgt cggcggtgtc gttctgcggc
     7681 gtgtgccaca ggttgaggtc cacgtagaag tcaagcgagc acagggcctc ccaggcgcgc
     7741 gtggtgttcg tctggctcat gaagttgccg gactcgttcc agagggcgcg caccgggtag
     7801 gggtcgcccg tgatgacggc gtcccacagc gaatggctgt cgcaccagta ctgccaccag
     7861 cccagaagcg ggaacttgtc gatgccgatc tgcttgcggt tgacttcctc cggcggggtg
     7921 gtggcgccgg gggcccaggc gctgaagccc tggaggtcgc cgtcgatggg gacgatggtc
     7981 gggccgcggt tgccgcccgg ggtgtccatg ttgccggtga tgcccacgag gttgtcgaag
     8041 gcgcggcagt tctggatggc gctgcagaag tgctcgaccg ccagcatgta ctggatgccg
     8101 ccgttgccgt agccggtgga cgggtcgatg cgcgtgccgt aggccgtggc cgccgcctcg
     8161 atctcggagg cggggatgcc ggtgatctcg gccgccacct cgggcttgta ctcggcggcg
     8221 cgggccttgt agtgctccca caccggcttg accttcacgg tcttgccgtc cttcagcgtg
     8281 atctcgaact cgccttccag cgcggggtcg atggcggggt cgaacggcat gggatcgggc
     8341 agccagccct gcgtctggcc cggcagcagg ttgtcctgcg cggcctcgcg gccttggcgc
     8401 gccttctcgg agtcgtagtt ctcgccctcc cagaagccgc cggtctcgtc gatgacgcct
     8461 tcctggtcgg cgttgaacca ggtgaacgcg ccgtactcgt gctcgacgcc ctcggccttc
     8521 agcttctccc agttgttgtc gtacacgagg aacttatagg ggctgccgcc ctccttgatg
     8581 tcgctttcct tgagcagcgc ggttttcacg tcccagtagg aaccgtcggt gcgcacggtg
     8641 gggaagccgg agggctccat gtcctcgcac acgaggaacg gggcgttcgt ccacttcttc
     8701 acgtacagct cgtcgtagag cttcttctcg atgatgacgt tcgtccaggc cagggccagg
     8761 gcgccgtcgg tgccggggcg caggtgctgc cagtagtcgg cttccttgcc catgttggcc
     8821 atgcgggggt cgacgctgat gtgcacgtcg gcgcgcgatg ccacgtccac cgtggtgcgg
     8881 caggagtcgt cgtagttgga cagctcggac gcgccgcccc actgcacgta cactttcggg
     8941 cgcgtgatgg tttccatcca cgacatggcg aactgcgaca ccatggtggt ggcgaagtgg
     9001 cgcgggccct tgcagatctg ccaggcctca acgttgttcg gcgtttccag catgttcttc
     9061 aggatggact ccgagtgcat gcaccagatg cgcgacgtgc ccacctggca ggcgatggcc
     9121 tcgccgccgt gcttcgcctt gatgtccatg aagttgtcca cgatggactg catggcctcg
     9181 tcccacgaga tgcgctgcca gccgggctcc tcgcccttcg ggttcgtgcg cttcatggga
     9241 tagtgcagac ggtcggggtg gtacgccgcc tggatggacg actgcgactt cgtgcagcag
     9301 ttgcccatgg actggaacgc tccctcgtcg ccctcgacgc gtatggcccg cccgttctgg
     9361 acgatgacct tgacgccgca ctccatcttg ccgcagccgc ggcagcacgt tttcacggcc
     9421 acggtgtcgt tgccggacac gctgctgtac gtctcttcgg ccagcgcggt gtgcgtcgaa
     9481 gcgccgaacg ccgcggctgc cgcaccggtg atggcggcgg tcttgacgaa cgtgcgtcgt
     9541 gacatggtca ggttacccat actcctcctc cttctttctc ctcgttctcg tcttgctcgt
     9601 cttcgttcgt gcgatcgggg aggggggctg ccgggtgaag cctcgggctc cgaaccggtg
     9661 caaagtcccc gtgtcgcttg ggccgaccat acggatgcga gggggtgcag agcatcgtcc
     9721 aaaggttgga aagcaggttc gaccgcatca taaattctta tgatcttgtg atcatgaaaa
     9781 attcgatagt gctttgaact ggggaaactt taaccgtagg tcataaatta ttctgaaact
     9841 agaatagttt cctcaaagag ggccgctcat cttttccgta taatcgaacc cgtcgacaga
     9901 ggggtcggcg cgagcgacgt tatgtacggg ctcacagggg gtggggcatg gaaaagcttc
     9961 ggagtgcatg ggataccgcg ttggggacga gcctttccct caagcgcatc gcgggcgcgg
    10021 ccttgcaatg gctttggatg ctcatgatgt tctacagcat cgtgccgtac ggcttctcgt
    10081 cggacgtgcg cggctcgctg tacgtcagcc tgatcatatc gctggtggcc atggtggtga
    10141 ccatgctgtt cgtctccgtc atcgtgcggc gcgagcgggt gagcggcaac cgctccatcg
    10201 tgctgggatc ggccatcatg atgagcgcgg gcagcgtgct cacgccgttc tccgatccta
    10261 ccacgcccgt cggcatgctg gtgctgggcc tgagcgcggt catgaccggc acgggctcgg
    10321 ccgtgctgtt cttgtgctgg atcgagctcg agtcggggct gggaggccgc ttggcgcttg
    10381 tggagcttgc ctcgtcgctg tgcatcgcgt tcgtcgtggg gttcttgctg atcgtagggc
    10441 cggcgatacc cgtcatcgtg ctcatcgttg tcatgcccgt gctttcggcg gcagctctca
    10501 ggcgcagcgc gcccgatacg ccgcagctgc cgcgcgagcc cgagcagcct ttgtcgcgcc
    10561 atacggcgac cctgttcgcg aaggcgcttc tgggcgcggt gctcatcggc atgttgatgg
    10621 ggttcttcga cgtggtgtcg gggttcaaaa cctacgaggt gcaggacatc ttcggcacgt
    10681 acctgtttct ggcgggcttc atcggagcgt tggccatctg cctgatagcc gtgttcctgc
    10741 agcgcgacag cgtgttcttc tcgtaccgcg tctccatgct gatgctgtgc ttgggatgct
    10801 tgtccacgcc gttcatggcc gacaacaaca cgtattcgag cgcgcttatc ttcggcggct
    10861 accactgctt cgtgctggtg ctgtgcgtgg tgtgcatcga cgtggcgtcc agcttccgcg
    10921 tgagcccggc gcggacgatc ggcctggggt tcgtggcgct gtacggcggc gagaccgcag
    10981 gccagctgtt cgcccattcg ctggaggcga ccggggtcag catgttcgac ctcacgctgg
    11041 tcacgctggt ggccgtgtcg ctgctgttca tcgcgcattt gttcctgttc acggagaccg
    11101 acctcatcaa aatcggcatc ggcgaggtca gcctgatggg cacggtgccc gaagaggccg
    11161 ccgacgaccc ggccgacccg gtggatccgt gcgcgctgat cgcggagcgc ttcgccctgt
    11221 cgccgcgcga gaccgacgtg cttccgctgc tgctggaggg ccgcaccatc tcgcgcatcc
    11281 aggagacgct gttcatctcg gcgggcacgg tgagcacgca tatccgccac atctaccaga
    11341 agacgggcgt ggacaatcgc caggagctca tcgacctggc ccacgagacg gccgcgcagg
    11401 acgaggcggt ctgaatctag aagcggccgc gaattcagaa tagtaataca ggatccgaat
    11461 cgtttcagtt gccgccggat taataatcaa ataaccgttc ctaaaaaatg ccctcttggg
    11521 ttatcaagag ggtcattata tttcgcaaaa aaccccgctt cggcggggtt ttttcgcctt
    11581 gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg
    11641 tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
    11701 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
    11761 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
    11821 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
    11881 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
    11941 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc
    12001 tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca
    12061 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg
    12121 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
    12181 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca
    12241 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
    12301 acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa
    12361 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
    12421 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
    12481 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc
    12541 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
    12601 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
    12661 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
    12721 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
    12781 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
    12841 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
    12901 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
    12961 tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
    13021 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
    13081 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
    13141 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
    13201 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
    13261 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
    13321 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
    13381 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ccgagatgcg ccgcgtgcgg
    13441 ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca
    13501 gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc
    13561 cgccggcatg agctcctcta gtagagagcg ttcaccgaca aacaacagat aaaacgaaag
    13621 gcccagtctt tcgactgagc ctttcgtttt atttgaaaaa gaaaagcccc agcaagcttg
    13681 gcggcagcag gggcattcga gacaaggaga ttatagcatg gcggc
//
