LOCUS       sequence-375941-        5333 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  ceh-24 fluorescent neural reporter driving nuclear CYOFP expression 
            (refer to NeuroPAL paper for expression).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5333)
  AUTHORS   Yemini E, Lin A, Nejatbakhsh A, Varol E, Sun R, Mena GE, Samuel ADT,
            Paninski L, Venkatachalam V, Hobert O
  TITLE     NeuroPAL: A Multicolor Atlas for Whole-Brain Neuronal Identification
            in C. elegans.
  JOURNAL   Cell. 2021 Jan 7;184(1):272-288.e11. doi: 
            10.1016/j.cell.2020.12.012. Epub 2020 Dec 29.
  PUBMED    33378642
REFERENCE   2  (bases 1 to 5333)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.12.012"; journalName: "Cell"; date: "2021-01-7-
            7"; volume: "184"; issue: "1"; pages: "272-288.e11"
COMMENT     Sequence Label: pEY69
FEATURES             Location/Qualifiers
     source          1..5333
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     6..22
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6..22
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             692..712
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             734..754
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(2924..2946)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     3046..3065
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(3143..3161)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3229..3333
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3334..3402,3403..4194)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3552..3571)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4365..4953
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4854..4873
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5107..5124
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5241..5262
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5277..5282,5283..5300,5301..5307)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5315..5331
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     join(5320..5333,1..9)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 tcacacagga aacagctatg accatgatta cgccaagctg taagtttaaa catgatctta
       61 ctaactaact attctcattt aaattttcag agcttaaaaa tggctgaaat cactcacaac
      121 gatggatacg ctaacaactt ggaaatgaaa taagcttgca tgcctgcagt ctcaagtggt
      181 aaccgccctc tggtatcaaa gcgatcctaa cgagccatcc tatatcggtg gtcctccgtc
      241 acaattaggc gtaaccaatg ataattgaga aaaagagtgt ggaagagatg gagacgaaga
      301 cagctcagcc acttagacgg taatcaacaa gtgtttggaa cacaatgaat tgaaaaagag
      361 gaggagaatc gagaattgta ttgaaattct tttgagcaga ttcattcagg tttaaaatca
      421 ttttaattga cacatttaag taaattttgt cacattttat agttgaaaca actttttggt
      481 ttaatttaaa agattaacac tcattatcag tttttttttc aatttctagg tttgtggatt
      541 acactttcag agacaacacg aataaacctg acagtatccc gggattggcc aaaggaccca
      601 aaggtatgtt tcgaatgata ctaacataac atagaacatt ttcaggagga cccttgcttg
      661 gagggtaccg agctcagaaa aaatgaccgc tccaaagaag aagcgcaagg ttccagtcga
      721 gaagatgact gccccaaaaa agaaacgtaa ggtcccgcgg gtaagtttaa acagttcggt
      781 actaactaac catacatatt taaattttca gcgcgctgtg tcaaaaggag aagagcttat
      841 taaggagaat atgcgttcca agctctacct cgagggatcc gtcaacggac accaattcaa
      901 gtgcacccac gagggagagg gaaagccata cgagggaaag caaaccaacc gtatcaaggt
      961 cgtcgaggga ggaccactcc cattcgcctt cgacatcctc gccacccact tcatgtacgg
     1021 atccaaggta agtttaaaca tatatatact aactaaccct gattatttaa attttcaggt
     1081 cttcatcaag tacccagccg acctcccaga ctacttcaag caatccttcc cagagggatt
     1141 cacctgggag cgtgtcatgg tcttcgagga cggaggagtc ctcaccgcca cccaagacac
     1201 ctccctccaa gacggagagc tcatctacaa cgtcaaggtc cgtggagtca acttcccagc
     1261 caacggacca gtcatgcaaa agaagaccct cggatgggag ccatccaccg agaccatgta
     1321 cccagccgac ggaggactcg agggacgttg cgacaaggta agtttaaaca tgattttact
     1381 aactaactaa tctgatttaa attttcaggc cctcaagctc gtcggaggag gacacctcca
     1441 cgtcaacttc aagaccacct acaagtccaa gaagccagtc aagatgccag gagtccacta
     1501 cgtcgaccgt cgtctcgagc gtatcaagga ggccgacaac gagacctacg tcgagcaata
     1561 cgagcacgcc gtcgcccgtt actccaacct cggaggagga atggacgagc tctacaagcc
     1621 taggataccg gtgggtgaag acccaccaaa gccatctgcc aagggagcca agaaggccgc
     1681 caagaccgtt acgaagccaa aggacggaaa gaagagacgt catgcccgta aggaatcata
     1741 ctccgtctac atctaccgtg tcctcaagca agttcatcca gacactggag tttcctccaa
     1801 agccatgtct atcatgaact cttttgtcaa cgatgtcttc gagcgtattg ctgctgaagc
     1861 atcccgtctt gctcactaca acaagcgttc cacaatctca tcccgcgaaa ttcagaccgc
     1921 tgtccgtctg atccttccag gagagcttgc caagcacgcc gtgtctgagg gaaccaaggc
     1981 cgttaccaag tacacttcca gcaagtagga attccaactg agcgccggtc gctaccatta
     2041 ccaacttgtc tggtgtcaaa aataataggg gccgctgtca tcagagtaag tttaaactga
     2101 gttctactaa ctaacgagta atatttaaat tttcagcatc tcgcgcccgt gcctctgact
     2161 tctaagtcca attactcttc aacatcccta catgctcttt ctccctgtgc tcccaccccc
     2221 tatttttgtt attatcaaaa aacttctctt aatttctttg ttttttagct tcttttaagt
     2281 cacctctaac aatgaaattg tgtagattca aaaatagaat taattcgtaa taaaaagtcg
     2341 aaaaaaattg tgctccctcc ccccattaat aataattcta tcccaaaatc tacacaatgt
     2401 tctgtgtaca cttcttatgt tttttacttc tgataaattt ttttgaaaca tcatagaaaa
     2461 aaccgcacac aaaatacctt atcatatgtt acgtttcagt ttatgaccgc aatttttatt
     2521 tcttcgcacg tctgggcctc tcatgacgtc aaatcatgct catcgtgaaa aagttttgga
     2581 gtatttttgg aatttttcaa tcaagtgaaa gtttatgaaa ttaattttcc tgcttttgct
     2641 ttttggggtt tcccctattg tttgtcaaga tttcgaggac ggcgtttttc ttgctaaaat
     2701 cacaagtatt gatgagcacg atgcaagaaa gatcggaaga aggtttgggt ttgaggctca
     2761 gtggaaggtg agtagaagtt gataatttga aagtggagta gtgtctatgg ggtttttgcc
     2821 ttaaatgaca gaatacattc ccaatatacc aaacataact gtttcctact agtcggccgt
     2881 acgggccctt tcgtctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc
     2941 tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg
     3001 gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa ctatgcggca tcagagcaga
     3061 ttgtactgag agtgcaccat atgcggtgtg aaataccgca cagatgcgta aggagaaaat
     3121 accgcatcag gcggccttaa gggcctcgtg atacgcctat ttttataggt taatgtcatg
     3181 ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct
     3241 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
     3301 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
     3361 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
     3421 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
     3481 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
     3541 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc
     3601 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
     3661 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
     3721 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
     3781 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
     3841 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc
     3901 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
     3961 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
     4021 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
     4081 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
     4141 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
     4201 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     4261 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     4321 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     4381 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     4441 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     4501 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     4561 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     4621 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     4681 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     4741 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     4801 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     4861 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     4921 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     4981 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
     5041 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
     5101 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc
     5161 cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg
     5221 ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta
     5281 cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca att
//
