LOCUS       sequence-375943-        7877 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  E. coli - Eggerthella lenta shuttle plasmid (TetR), dadR(Delta-TM) 
            complementation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7877)
  AUTHORS   Dong X, Guthrie BGH, Alexander M, Noecker C, Ramirez L, Glasser NR, 
            Turnbaugh PJ, Balskus EP
  TITLE     Genetic manipulation of the human gut bacterium Eggerthella lenta 
            reveals a widespread family of transcriptional regulators.
  JOURNAL   Nat Commun. 2022 Dec 9;13(1):7624. doi: 10.1038/s41467-022-33576-3.
  PUBMED    36494336
REFERENCE   2  (bases 1 to 7877)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-12-9"; volume: "13(1):7624. doi"; pages: " 
            10.1038/s41467-022-33576-3"
COMMENT     Sequence Label: pXD70Tet(DadRmt2)-DadR(Delta-TM)
FEATURES             Location/Qualifiers
     source          1..7877
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(4983..5010)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     misc_feature    5595..5614
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that start with ""ATG"""
     primer_bind     complement(5771..5789)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5857..5961
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5962..6030,6031..6822)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6180..6199)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6993..7581
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7482..7501
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(7762..7833)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 agacaaggag attatagcat ggcggcaaac gctggcgaga cgaaggtgaa gctctgggag
       61 ggcatctgct acccagagaa catgcgcgat gactggcagg acgagataga cgacattctt
      121 caagtgcctt tcgcttatgc ggttcacgac atagaccatg accagaagag caagcagcgc
      181 aagacgcatg cgcacgtcat tgttgtctgg ggcggcaaca cgacacgcaa ggcaatcatc
      241 aacgtcttga accgattgag cgcggacggc aagaagtgct gttcgtcagc cgagccaatc
      301 aacaacatca ggcatgcgta tgactatctg attcacgaca cggcttcctg tcgcaagaag
      361 ggcaaggagc tgtatccggt cgaagcacgc atcgagggaa acaacttcga cattggtcag
      421 ctcgaacagc tctcgactaa ggacaagcag gacatgctct tcgagttggt cggcttcatc
      481 atgtgcgaga ggttcgagac catcaacgat ttcacggctg cggcgctgag ggagtttccc
      541 gagcagtacc gcgaaatcat cgtggggtac aactcgattc tcgagcgcta ttgtcgcggg
      601 aactatctga acgccgaacg taagcgcaag tgcgtggaac agtcaggtgc caaagagtag
      661 tcgggtcaat gcagcaatgc gtgactacgg ggcggcgtgg tctgcgctcg cgcagatcta
      721 agccgactcg ttcacggatt gcggaattga cgcgacggcc aaccgaatag accagacttg
      781 attaagggtc gatggacgct ctgctccatc gaccctttcc ttgcccgtgt ctcgactgga
      841 tgagacctgc agaatcgggc ggctcattat ttttcgctaa ctacgtccga aaaataatgt
      901 gtccgaaaaa taatgtgaca aatgacaaat gacaaatgac cacagggggg ccactacgac
      961 ggccccccta atagtgccgt gtgccgtgtg ccgaaccgca ggtagattga ggggtggttc
     1021 ggaggggcga agtccctccg ttcggcgctc gcgccgatgg cccgagggcc gtgagcgaag
     1081 ccgcgaacgg gcaagggctg gggggtctcg gggggaacgc ccgagcgggt gcagggctgg
     1141 ccctgccacg tccttagcgt agctaaggtc taacgtgcta gactttagtc gattaagtct
     1201 gtacggttcg gaggtttcca tggctcacat cgcgaaatac aaggctccat cggtcgggca
     1261 catgcttgcc cactaccgcc gcgaccgcag cagcctggag cgcgacaaca tcgaccccga
     1321 gcgcatcaag aacgacttgg tggtcggcca ctacacgaac aaggacggca agctcgttgt
     1381 ggggcgcgtg gagccgcgcg atggcgagcc gaactggggc acggtggaga gccgcatcga
     1441 gcgcgtgaac gaggcccaga aggccgcagg gaagcgtgcc acgcgcaagg atgcggtcgt
     1501 tatggctgac gttgttgtga cgcttcccga caatgtgcgc aagggcgatg aggacaggtt
     1561 cttccgtctc acgtactggt acctgtcgaa gaagttcggc atcgagaaca tgatgggcgg
     1621 gtacgtccac aaggacgagg tgttgaagga tggcacgccc gcccgcgacc acatgcacgt
     1681 gccgttcacg cccatcctag atggacggtt caactacaag cagatgtgcc cccgcagctt
     1741 ctaccggagc atgcacaagg agcttgggga ttacctagag gggcgcatgg gctaccgccc
     1801 tgcaatcgag cttgacgagg agacgcgggc gcagcgtgta tacacggaca agtccgtgga
     1861 tatcgacaag gtacgctgtg cggtcgataa ggccgtggtt gaacctgcga aggacgaagc
     1921 ggcgcgaatc gtcgcggcgg ctagggaaga agccgccgct ttactgaatg atgccgaaac
     1981 gcgtaaggca gagcttgtta ccgagattgc cgacaaggag ggtgaccttg ccgagcttga
     2041 cagccagctc gaggacgtga agctcgatat cgaagatgag caagaccgat tggagtgtct
     2101 tcggcaacga gcggacggag ttgcgcgaga cattggagag cttcaaccca tcgctaccga
     2161 ggttcggggc tgggaagctg cgggaaaagc tgagcgcggc gcaatcctcg ataacatcgt
     2221 tgttaagtgc gatggactcg cgagccgcat cagagcagga gttgcgggaa tgcgaatcaa
     2281 agtggaagag cttagaagtc gaatatcgcg cccgctcgaa tatctgatgc gcagggaaca
     2341 gccaaggcaa cagagcctta atgacgtgct gcgcgatgcg cagcgggcgg cggatgcgtg
     2401 gaaccgcgac cacgctgcac cgcagaggac gtatcgcggg cgggctagat gatgtgttat
     2461 agtagtacct gtaatacacg acaaggaggt gcgacgcgaa aaaaccccgc tgaagcgggg
     2521 ttttttgcgc accacagcag acagcagaaa acattctgaa cgctaggaag ccggtatgat
     2581 tgttacatat aaggggaaga aaaatttctt ttaggtactt gctttcctaa aactgatgtg
     2641 atacaatgat ttaatccaga aaaggagtaa aaaatatgcg gcaaggtatt cttaaataaa
     2701 actataatca aatagtggga acaaaggatt atgatagctc cttttgtagg gacttagttt
     2761 tttgtaccca atttaagaat acttttgcct tatcaatttt gacatatccc caaaaacagc
     2821 aatcacaaac aggtgtatgc tgtatatgtg tatgtccgca acttataatc cccagtggta
     2881 aaagtatttt actgctgggg atttttatgc cctttggggc tgtaaaggga ggacaatcac
     2941 atgaaaataa tcaatattgg aattcttgcc catgtagacg ctggaaagac gaccttgacg
     3001 gagagcctgc tatatgccag cggagccatt tcagaaccgg ggagcgtcga aaaagggaca
     3061 acgaggacgg acaccatgtt tttggagcgg cagcgtggga ttaccattca agcggcagtc
     3121 acttccttcc agtggcacag atgtaaagtt aacattgtgg atacgcccgg ccacatggat
     3181 tttttggcgg aggtgtaccg ctctttggct gttttagatg gggccatctt ggtgatctcc
     3241 gctaaagatg gcgtgcaggc ccagacccgt attctgttcc atgccctgcg gaaaatgaac
     3301 attcccaccg ttatctttat caacaagatc gaccaggctg gcgttgattt gcagagcgtg
     3361 gttcagtctg ttcgggataa gctctccgcc gatattatca tcaagcagac ggtgtcgctg
     3421 tccccggaaa tagtcctgga ggaaaatacc gacatagaag catgggatgc ggtcatcgaa
     3481 aataacgatg aattattgga aaagtatatc gcaggagaac caatcagccg ggaaaaactt
     3541 gcgcgggagg aacagcagcg ggttcaagac gcctccctgt tcccagtcta tcatggcagc
     3601 gccaaaaatg gccttggcat tcaaccgttg atggatgcgg tgacagggct gttccaaccg
     3661 attggggaac aggggggcgc cgccctatgc ggcagcgttt tcaaggttga gtacaccgat
     3721 tgcggccagc ggcgtgtcta tctacggtta tacagcggaa cgctgcgcct gcgggatacg
     3781 gtggccctgg ccgggagaga aaagctgaaa atcacagaga tgcgtattcc atccaaaggg
     3841 gaaattgttc ggacagacac cgcttatcag ggtgaaattg ttatccttcc cagcgacagc
     3901 gtgaggttaa acgatgtatt aggggaccaa acccggctcc ctcgtaaaag gtggcgcgag
     3961 gaccccctcc ccatgctgcg gacggcgatt gcgccgaaaa cggcagcgca aagagaacgg
     4021 ctgctggacg ctcttacgca acttgcggat actgacccgc ttttgcgttg cgaagtggat
     4081 tccatcaccc atgagatcat tctttctttt ttgggccggg tgcagttgga ggttgtttcc
     4141 gctttgctgt cggaaaaata caagcttgaa acagtggtaa aggaaccctc cgtcatttat
     4201 atggagcggc cgctcaaagc agccagccac accatccata tcgaggtgcc gcccaacccg
     4261 ttttgggcat ccataggact gtctgttaca ccactctcgc ttggctccgg tgtacaatac
     4321 gagagccggg tttcgctggg atacttgaac cagagttttc aaaacgctgt cagggatggt
     4381 atccgttacg ggctggagca gggcttgttc ggctggaacg taacggactg taagatttgc
     4441 tttgaatacg ggctttatta cagtccggtc agcacgccgg cggacttccg ctcattggcc
     4501 ccgattgtat tggaacaggc attgaaggaa tcggggacgc agctgctgga accttatctc
     4561 tccttcatcc tctatgcgcc ccaggaatac ctttccaggg cttatcatga tgcaccgaaa
     4621 tactgtgcca ccatcgaaac ggcccaggta aaaaaggatg aagttgtctt tactggcgag
     4681 attcccgccc gctgtataca ggcataccgt actgatctgg ccttttacac caacgggcgg
     4741 agcgtatgcc ttacagagct gaaaggatat caggccgctg tcggtcagcc ggtcatccag
     4801 ccccgccgtc caaacagccg cctggacaag gtgcgccata tgtttcagaa ggtaatgtaa
     4861 atcgttaagg gatcaacttt gggagagagt tcaaaattga tccttttttt ataacaggaa
     4921 ttaaataata aaaaagccgg attaataatc tggcttttta tattctctct ctagtatata
     4981 aacgcagaaa ggcccacccg aaggtgagcc agtgtgagtc gactagttca gaccgcctcg
     5041 tcctgcgcgg ccgtctcgtg ggccaggtcg atgagctcct ggcgattgtc cacgcccgtc
     5101 ttctggtaga tgtggcggat atgcgtgctc accgtgcccg ccgagatgaa cagcgtctcc
     5161 tggatgcgcg agatggtgcg gccctccagc agcagcggaa gcacgtcggt ctcgcgcggc
     5221 gacagggcga agcgctccgc gatcagcgcg cacggatcca ccgggtcggc cgggtagtcg
     5281 gcggcctctt cgggcaccgt gcccatcagg ctgacctcgc cgatgccgat tttgatgagg
     5341 tcggtctccg tcatgcccca ccccctgtga gcccgtacat aacgtcgctc gcgccgaccc
     5401 ctctgtcgac gggttcgatt atacggaaaa gatgagcggc cctctttgag gaaactattc
     5461 tagtttcaga ataatttatg acctacggtt aaagtttccc cagttcaaag cactatcgaa
     5521 tttttcatga tcacaagatc ataagaattt atgatgcggt cgaacctgct ttccaacctt
     5581 tggacgatgc tcatctagaa gcggccgcga attcagaata gtaatacagg atccgaatcg
     5641 tttcagttgc cgccggatta ataatcaaat aaccgttcct aaaaaatgcc ctcttgggtt
     5701 atcaagaggg tcattatatt tcgcaaaaaa ccccgcttcg gcggggtttt ttcgccttga
     5761 agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt
     5821 tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
     5881 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
     5941 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
     6001 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
     6061 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
     6121 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
     6181 ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata
     6241 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
     6301 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc
     6361 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
     6421 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
     6481 gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact
     6541 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
     6601 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
     6661 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc
     6721 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
     6781 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
     6841 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
     6901 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     6961 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     7021 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     7081 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc
     7141 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     7201 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     7261 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     7321 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     7381 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     7441 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     7501 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     7561 ggggggcgga gcctatggaa aaacgccagc aacgcggccc gagatgcgcc gcgtgcggct
     7621 gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt
     7681 tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg
     7741 ccggcatgag ctcctctagt agagagcgtt caccgacaaa caacagataa aacgaaaggc
     7801 ccagtctttc gactgagcct ttcgttttat ttgaaaaaga aaagccccag caagcttggc
     7861 ggcagcaggg gcattcg
//
