LOCUS       sequence-375951-        6904 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  gcy-35 fluorescent neural reporter driving nuclear mNeptune2.5 
            expression (refer to NeuroPAL paper for expression).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6904)
  AUTHORS   Yemini E, Lin A, Nejatbakhsh A, Varol E, Sun R, Mena GE, Samuel ADT,
            Paninski L, Venkatachalam V, Hobert O
  TITLE     NeuroPAL: A Multicolor Atlas for Whole-Brain Neuronal Identification
            in C. elegans.
  JOURNAL   Cell. 2021 Jan 7;184(1):272-288.e11. doi: 
            10.1016/j.cell.2020.12.012. Epub 2020 Dec 29.
  PUBMED    33378642
REFERENCE   2  (bases 1 to 6904)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.12.012"; journalName: "Cell"; date: "2021-01-7-
            7"; volume: "184"; issue: "1"; pages: "272-288.e11"
COMMENT     Sequence Label: pEY45
FEATURES             Location/Qualifiers
     source          1..6904
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     6..22
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6..22
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2579..2599
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2624..2644
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2666..2686
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(4495..4517)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     4617..4636
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(4714..4732)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4800..4904
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4905..4973,4974..5765)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5123..5142)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5936..6524
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6425..6444
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    6812..6833
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6848..6853,6854..6871,6872..6878)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6886..6902
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     join(6891..6904,1..9)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 tcacacagga aacagctatg accatgatta cgccaagctg taagtttaaa catgatctta
       61 ctaactaact attctcattt aaattttcag agcttaaaaa tggctgaaat cactcacaac
      121 gatggatacg ctaacaactt ggaaatgaaa taagcttgca tgcctgcagt ttccgcatat
      181 agcttatagc attagaaaaa aaaattttga ttcttgagaa ttgaaattag gaggataaca
      241 ttttttttta taaaatgtta tttaattttc agtttttttt tggaaatctt tcaaatctga
      301 ttatctcctt agtggtttga gatacaaaaa agttgtaaac tgacaaaata tttgccataa
      361 aatttttaac attttcctag tgaacaagac atttgtagct tctacgcaaa taaagttatg
      421 aaggtttcaa cattgctagc ccttccaggt ggaggaaaat aagagaggga gtgtgaggtg
      481 aggggagagc gcagacagct agggcactct cgtttctctg tactctcccc tccagtattt
      541 gctattctag agtcttgaga aagaacaaag aaactgattt ttttacagat agaacatttg
      601 aggactacgt ttttttccaa atgtttcaac aaaaaaattc agtacttatt ttttcaaatt
      661 tttcataatg ctacaaaaat tttgccaact ataaaaatgt ccaaattgtt tgctttttcc
      721 agtaaaaaat ttccctttat tgctaaccat atccgagata tttattagag cttgaaattt
      781 ccaggctacc tacctaaatc cgcaaaatct cacacaaagc tagatttgtg ctcattgttg
      841 agctcataaa aacaaaataa agaaaataaa aacaaagtaa tttcagagag agaaaggagc
      901 acgaagaaga agccttcggg ggatgggaga gagagagggc gagtctcttc ttttcacatc
      961 acacaacaca aaaaaacgaa gcgaacacat gttagcatca attgagcatc ttttttgttg
     1021 tctagccgtc tgtctgtgtg tcggttcata gggggaccta cgcctgccta cgtgtctagg
     1081 tggttaaaaa ttgaaaatat agattttttc aatttttttg tttttttttc agacaaaaaa
     1141 tcggtaaaaa ttgaatcgca aaaattttat cttatcgaag tcacgtgatg tcaggctgtc
     1201 caattacgat ttgatctaca taaaacgcgg gaattttttg caaaaaaaac gtgacgtcag
     1261 cacgttctta accatgcgaa atcagtttag aactctgcgt ctcttctccc gcattttttg
     1321 tagatctacg tagatcaaac cgaaatgaga cactctgaca ccacatgaaa agttgaaatt
     1381 atcgattttt ttttcaagtt aattataaat tttaaaaaag gtgaaaaagt aaataaactt
     1441 ttcagttttt tttcaatttt ttccaaaaca attttaggtc catcggaata ttataaacca
     1501 tttgacaaaa aaatttgaag gagagacgca gacggagaga gtactctcgt ttctctgcgc
     1561 tctccctgcc tctcagagcc agctcgagct catcaaatag ctcattcgaa taaaaatgag
     1621 cactttttgt gctcattcag gacattgcat tttatgctcc actgaaccta aaaattttcg
     1681 aagatcaatg ggaatttcca atgaagtttt ttttttgaaa aaaaaatgat ggaaaatttg
     1741 atgatctgtg aaaatcattt tgagagaaga aaatttgtag aaatgttttc aaaaaatcgg
     1801 tttttttcca gaattcttgc aaaaaaaaat ttagaaaaaa ctttgagaaa aaatctaaaa
     1861 attattttga ggaaatgaca ggggattagc atgccgctcc atcattttct tctccaaaat
     1921 aatcctaaaa ttagtccaaa ccgcaaaaaa ccagattttt tgtcattcta aaaaattgcg
     1981 ggaggcgtgg tgtccttttc cataagccta taccccccaa acatcaaatt tccggtcgga
     2041 agagtcagcc gacatttccc caaatatata tgtgaatgaa aaaaaaagta gggagaattg
     2101 aataattcat gaggctgatt gagaaacaat ggggtgggca cagatagatt ttctgttaat
     2161 attgagaatc caaacacagg caaaaagcat aatgcgttcg gggggaatcg aaccccccag
     2221 aatcctatca tcatcataaa ttctgtgtca tctcccttct tccatccaat ttcttcatgt
     2281 tatcatgcga aaagtggttg tgacgaatgg ctaatgatca tctgacacgc aatggctccc
     2341 cgccaccccg actcgtttca aacttacacc caaagtcttc atatttggaa tttttgaaaa
     2401 atatatatta tatacgttac ttttttgcgg agagtagaat cccgggattg gccaaaggac
     2461 ccaaaggtat gtttcgaatg atactaacat aacatagaac attttcagga ggacccttgg
     2521 agggtaccgg ttttcccagt cacgacgcgt ggtaccgagc tcagaaaaaa tgaccgctcc
     2581 aaagaagaaa cgcaaagtac cgagctcaga aaaaatgacg gcacctaaaa agaaacgcaa
     2641 agtaccggtc gccgagaaga cagccccaaa aaagaagcgt aaagttccac gcgtcatggg
     2701 agtaagttta aacagttcgg tactaactaa ccatacatat ttaaattttc agcgcgctgt
     2761 ctccaaggga gaggagctca tcaaggagaa catgcacacc aagctctaca tggagggaac
     2821 cgtcaacaac caccacttca agtgcaccca cgaaggggag ggcaagccct acgagggcac
     2881 ccagaccaac aggattaagg tggtggaggg aggccccctg ccgttcgcat tcgacatcct
     2941 ggccacctgc tttatgtacg ggagcaagac cttcatcaac cacacccaag gaatcccaga
     3001 cttcttcaag caatccttcg taagtttaaa catgatttta ctaactaact aatctgattt
     3061 aaattttcag cctgagggct tcacatggga gagagtcacc acatacgaag acgggggcgt
     3121 gcttaccgtt acccaggaca ccagcctcca ggacggctgc ttgatctaca acgtcaagct
     3181 cagaggggtg aacttcccat ccaacggccc tgtgatgcag aagaaaacac tcggctggga
     3241 ggccagtacc gagaccctgt accccgctga cggcggcctg gaaggcagat gcgacatggc
     3301 cctgaagctc gtgggcgggg gccacctgca ctgcaacctg aagaccacat acagatccaa
     3361 gaaacccgct aagaacctca agatgccagg agtaagttta aacatatata tactaactaa
     3421 ccctgattat ttaaattttc aggtctactt tgtggaccgc agactggaaa gaatcaagga
     3481 ggccgacaat gagacctacg tcgagcagca cgaggtggct gtggccagat actgcgacct
     3541 ccctagcaaa ctggggcaca aacttaatgg catggacgag ctgtacaagt aagcgccggt
     3601 cgctaccatt accaacttgt ctggtgtcaa aaataatagg ggccgctgtc atcagagtaa
     3661 gtttaaactg agttctacta actaacgagt aatatttaaa ttttcagcat ctcgcgcccg
     3721 tgcctctgac ttctaagtcc aattactctt caacatccct acatgctctt tctccctgtg
     3781 ctcccacccc ctatttttgt tattatcaaa aaacttctct taatttcttt gttttttagc
     3841 ttcttttaag tcacctctaa caatgaaatt gtgtagattc aaaaatagaa ttaattcgta
     3901 ataaaaagtc gaaaaaaatt gtgctccctc cccccattaa taataattct atcccaaaat
     3961 ctacacaatg ttctgtgtac acttcttatg ttttttactt ctgataaatt tttttgaaac
     4021 atcatagaaa aaaccgcaca caaaatacct tatcatatgt tacgtttcag tttatgaccg
     4081 caatttttat ttcttcgcac gtctgggcct ctcatgacgt caaatcatgc tcatcgtgaa
     4141 aaagttttgg agtatttttg gaatttttca atcaagtgaa agtttatgaa attaattttc
     4201 ctgcttttgc tttttggggt ttcccctatt gtttgtcaag atttcgagga cggcgttttt
     4261 cttgctaaaa tcacaagtat tgatgagcac gatgcaagaa agatcggaag aaggtttggg
     4321 tttgaggctc agtggaaggt gagtagaagt tgataatttg aaagtggagt agtgtctatg
     4381 gggtttttgc cttaaatgac agaatacatt cccaatatac caaacataac tgtttcctac
     4441 tagtcggccg tacgggccct ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg
     4501 acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca
     4561 agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta actatgcggc
     4621 atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt
     4681 aaggagaaaa taccgcatca ggcggcctta agggcctcgt gatacgccta tttttatagg
     4741 ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc
     4801 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     4861 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
     4921 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
     4981 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
     5041 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
     5101 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
     5161 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
     5221 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
     5281 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
     5341 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
     5401 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
     5461 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
     5521 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
     5581 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
     5641 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
     5701 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
     5761 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
     5821 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     5881 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     5941 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     6001 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     6061 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
     6121 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     6181 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     6241 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     6301 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     6361 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     6421 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     6481 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     6541 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     6601 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
     6661 gccgaacgac cgagcgcagc gagtcagtga gcgatgaagc ggaagagcgc ccaatacgca
     6721 aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg
     6781 actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac
     6841 cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac
     6901 aatt
//
